KLF9 in cancer: a potential prognostic marker and therapeutic target.

Kruppel-like factor 9 (KLF9) is a transcription factor that has gained significant attention in recent years for its critical involvement in development and progression of various cancers. Recent research has revealed the dual nature in tumorigenesis, where KLF9 can function as either a tumor suppressor or an oncogene, depending on the cellular context. Clinically, KLF9 emerges as a potential prognostic biomarker due to its differential expression patterns across various cancer types, with lower KLF9 levels often correlating with poorer patient outcomes.

Reduced Cortical Surface Area in the Frontal Operculum as a Causal Risk Predictor for Chronic Pain.

Chronic pain is a prevalent and debilitating condition that imposes substantial personal and societal burdens. Despite its significance, the neural mechanisms underlying individual susceptibility to chronic pain remain inadequately understood. In this study, we examined the prospective associations between 1325 brain structural imaging phenotypes and the future risk of developing chronic pain in a UK Biobank cohort of 5754-5756 participants.

Multiset Variational Quantum Dynamics Algorithm for Simulating Nonadiabatic Dynamics on Quantum Computers.

Accelerating quantum dynamical simulations with quantum computing has received considerable attention but remains a significant challenge. In variational quantum algorithms for quantum dynamics, designing an expressive and shallow-depth parametrized quantum circuit (PQC) is a key difficulty. Here, we propose a multiset variational quantum dynamics algorithm (MS-VQD) tailored for nonadiabatic dynamics involving multiple electronic states.

Quantum Computation of Conical Intersections on a Programmable Superconducting Quantum Processor.

Conical intersections (CIs) are pivotal in many photochemical processes. Traditional quantum chemistry methods, such as the state-average multiconfigurational methods, face computational hurdles in solving the electronic Schrödinger equation within the active space on classical computers. While quantum computing offers a potential solution, its feasibility in studying CIs, particularly on real quantum hardware, remains largely unexplored.

KLF9 regulates osteogenic differentiation of mesenchymal stem cells.

Osteoporosis is a progressive skeletal disease which is characterized by reduced bone mass and degradation of bone microstructure. Mesenchymal stem cells (MSCs) have the potential to inhibit osteoporosis since they are multipotent stem cells that can differentiate into multiple types of cells including osteoblasts. Hence the mechanism of osteogenic differentiation of MSCs deserves comprehensive study.

Characterization of the effects of outliers on ComBat harmonization for removing inter-site data heterogeneity in multisite neuroimaging studies.

Data harmonization is a key step widely used in multisite neuroimaging studies to remove inter-site heterogeneity of data distribution. However, data harmonization may even introduce additional inter-site differences in neuroimaging data if outliers are present in the data of one or more sites. It remains unclear how the presence of outliers could affect the effectiveness of data harmonization and consequently the results of analyses using harmonized data.

A Narrative Review of Different Hemostatic Materials in Emergency Treatment of Trauma.

Hemostatic materials are very important for the treatment of a large number of bleeding trauma patients in battlefield and disaster environments. Different types of hemostatic materials need to be used for emergency hemostasis according to different injury parts and severity. At present, the first-aid hemostatic materials have been well applied to the bleeding of body surface wounds, limbs, and junctions, but there are still no ideal hemostatic materials in the early treatment of first aid for the deep and incompressible bleeding of thoracoabdominal cavity and visceral organs.

Jmjd6 regulates ES cell homeostasis and enhances reprogramming efficiency.

Jmjd6 is a conserved nuclear protein which possesses histone arginine demethylation and lysyl hydroxylase activity. Previous studies have revealed that Jmjd6 is essential for cell differentiation and embryo development. However, the role of Jmjd6 in mammalian ES cell identity and reprogramming has been unclear.

A Novel Strategy toward Thermally Activated Delayed Fluorescence from a Locally Excited State.

It is well-known that thermally activated delayed fluorescence (TADF) is always generated from charge-transfer (CT) excited states in donor-acceptor (D-A) systems, which limits its application owing to a slow radiative process and a small stimulated emission cross section. Herein, a design strategy is proposed for realizing TADF from a locally excited (LE) state without a typical donor-acceptor type structure through controlling the intersystem crossing (ISC) and reverse intersystem crossing (RISC) processes between the lowest excited singlet with LE character and higher triplet states. Using this strategy, a boron difluoride derivative is theoretically predicted and experimentally synthesized to exhibit locally excited TADF (LE-TADF) with a fairly large radiative rate of 1.

Histone modifications in neurodifferentiation of embryonic stem cells.

Post-translational modifications of histone proteins regulate a long cascade of downstream cellular activities, including transcription and replication. Cellular lineage differentiation involves large-scale intracellular signaling and extracellular context. In particular, histone modifications play instructive and programmatic roles in central nervous system development.

Regulation of Thermally Activated Delayed Fluorescence to Room-Temperature Phosphorescent Emission Channels by Controlling the Excited-States Dynamics via J- and H-Aggregation.

Control of excited-state dynamics is key in tuning room-temperature phosphorescence (RTP) and thermally activated delayed fluorescence (TADF) emissions but is challenging for organic luminescent materials (OLMs). We show the regulation of TADF and RTP emissions of a boron difluoride β-acetylnaphthalene chelate (βCBF ) by controlling the excited-state dynamics via its J- and H-aggregation states. Two crystalline polymorphs emitting green and red light have been controllably obtained.

Differentiation of Human Parthenogenetic Embryonic Stem Cells into Functional Hepatocyte-like Cells.

Stem cell and tissue engineering-based therapies for acute liver failure (ALF) have been limited by the lack of an optimal cell source. We aimed to determine the suitability of human parthenogenetic embryonic stem cells (hPESCs) for the development of strategies to treat ALF. We studied the ability of human parthenogenetic embryonic stem cells (hPESCs) with high whole-genome SNP homozygosity, which were obtained by natural activation during in vitro fertilization (IVF), to differentiate into functional hepatocyte-like cells in vitro by monolayer plane orientation.

Breaking Kasha's Rule as a Mechanism for Solution-Phase Room-Temperature Phosphorescence from High-Lying Triplet Excited State.

Organic room-temperature phosphorescence (ORTP) has been demonstrated successfully in solids. In contrast, solution-phase ORTP is rarely achieved, because the T → S phosphorescence is too slow to compete against nonradiative decay and the oxygen-quenching effect. Here, we reported that suppression of Kasha's rule is a strategy to achieve solution-phase ORTP from the high-lying T state by spatially separating T and T on different parts of the molecule (CzCbDBT) composed of carbonyl (Cb), dibenzothiophene (DBT), and carbazole moiety (Cz).

Efficient Definitive Endoderm Differentiation from Human Parthenogenetic Embryonic Stem Cells Induced by Activin A and Wnt3a.

Objective: This study aimed to investigate the effects of combined activin A and Wnt3a treatment on definitive endoderm (DE) differentiation from human parthenogenetic embryonic stem cells (hPESCs).

Methods: hPESCs on human foreskin fibroblast feeder layers were induced to differentiate into DE using a combination of 50 ng/ml activin A and 25 ng/ml Wnt3a. Expression of the DE markers CXCR4, E-cadherin (ECD), Sox17, and Goosecoid (Gsc) were examined using flow cytometry and real-time quantitative PCR.

Remotely Photocontrolled Microrobots based on Photomechanical Molecular Crystals.

Creating nano-to-macroscopic-sized artificial actuators in response to light has been a challenging issue. Herein, we describe the design, synthesis, and operation of a photomechanical molecular crystal (PMMC) that exhibits well-controlled multiple photo-driven motions, including translation, rotation, and jumping, by adjusting the irradiation sites. Theoretical calculation discloses that conversion of light energy into macroscopic motion occurs through a molecular conformation change between the excited and ground states mediated by ultrafast conical internal conversion, making the photomechanical/recovery responses a rapid cycle.

Microfluidic-Based Mechanical Phenotyping of Androgen-Sensitive and Non-sensitive Prostate Cancer Cells Lines.

Cell mechanical properties have been identified to characterize cells pathologic states. Here, we report our work on high-throughput mechanical phenotyping of androgen-sensitive and non-sensitive human prostate cancer cell lines based on a morphological rheological microfluidic method. The theory for extracting cells' elastic modulus from their deformation and area, and the used experimental parameters were analyzed.

The comparison between force volume and peakforce quantitative nanomechanical mode of atomic force microscope in detecting cell's mechanical properties.

Atomic force microscope (AFM) has been widely used in the biological field owing to its high sensitivity (subnanonewton), high spatial resolution (nanometer), and adaptability to physiological environments. Nowadays, force volume (FV) and peakforce quantitative nanomechanical (QNM) are two distinct modes of AFM used in biomechanical research. However, numerous studies have revealed an extremely confusing phenomenon that FV mode has a significant difference with QNM in determining the mechanical properties of the same samples.