Climate warming reshapes seasonal flowering but stabilizes species interactions in a Tibetan alpine grassland.

Climate warming commonly drives asymmetric shifts in flowering phenology among species, potentially disrupting plant-plant interactions and threatening ecosystem stability. However, the mechanisms driving these species-specific phenological responses, and the extent to which resulting asynchrony destabilizes interspecific interactions, remain poorly understood. Using a 3-yr in situ warming experiment in a Tibetan alpine grassland, we monitored seasonal flowering patterns of 29 species and quantified interaction potentials across 812 species pairs from their flowering-time overlap.

Screening of hub genes and immunocytes related to tendon injury based on bioinformatics and machine learning models.

Tendon injury is a common and challenging clinical problem, and its healing process involves complex cellular and biological factors. Therefore, this study aims to reveal the mechanism of tendon healing and provide theoretical basis for clinical treatment. We first selected GSE26051 dataset from the GEO database and used R language to obtain 721 DEGs (459 up-regulated and 262 down-regulated).

Antibiotic Susceptibility Evaluation in Bacterial Infections from Orthopedic Inpatients with Leg Trauma.

The COVID-19 pandemic has profoundly impacted healthcare delivery systems worldwide, but its impact on orthopedic infection patterns remains unclear. This retrospective comparative study analyzed 173 patients with leg trauma-related orthopedic infections from June 2018 to May 2025, divided into two periods based on China's pandemic control policy changes in December 2022: pre-policy adjustment (n = 86) and post-policy adjustment (n = 87). Our study revealed four major pandemic-driven changes: First, patients were significantly younger (50.

Lightweight deep neural network for contour detection and extraction of wheat spikes in complex field environments.

Background: Spikelet number, a core phenotypic parameter for wheat yield composition, requires precise estimation through accurate spike contour extraction and differentiation between grain surfaces and spikelet surfaces. However, technical challenges persist in precise spike segmentation under complex field backgrounds and morphological differentiation between grain/spikelet surfaces.

Method: Building on two-year multi-angle wheat spike imagery, we propose an enhanced YOLOv9-LDS multi-scale object detection framework.

Stride-Wise Adaptive Assistance Strategy for Ankle Exoskeleton Under Varying Walking Conditions.

Efficient and effective personalized assistance strategies are crucial for enhancing exoskeleton performance under varying walking conditions. We proposed a novel real-time adaptive assistance strategy to generate personalized and stride-wise customized ankle exoskeleton assistance profiles that adjusted to diverse and varying human locomotion demands. This approach tuned the assistance magnitude and timing online, starting from a profile pre-optimized during medium constant-speed walking, based on real-time ankle momentum estimation.

miR-223 alleviates DSS-induced colitis by prompting macrophage M2 polarization through PPAR-γ/FOXO1 signaling.

Background: Macrophage polarization represents a promising therapeutic target for inflammatory bowel disease (IBD). This study investigates the role of miRNA-223 (miR-223) in dextran sodium sulfate (DSS)-induced colitis and its regulation of macrophage polarization.

Methods: Male C57BL/6 mice were assigned to four groups: Wild-type (WT) control, DSS-treated group (DSS), DSS+miR-223 agomir (DSS+A), and DSS+ miR-223 agomir negative control (DSS+NC).

Topology of Variants Linked With Islet Function and Higher Risk of Urological Symptoms in -Associated Disease.

Wolfram syndrome type 1 gene (), which encodes a transmembrane (TM) structural protein (wolframin), is essential for several biological processes. Mutations of , autosomal dominant or recessive inherited, are related to a broad clinical spectrum. Molecular genetic tests were performed, and clinical phenotypes of three WFS1-associated cases were evaluated.

Three-Dimensional-Printed Meat Products with Lycopene-Functionalized Yeast Pickering Emulsions as Fat Replacer.

Due to the health-driven demand for fat replacers in meat products, Lycopene (Lyc)-loaded yeast protein (YP) high internal phase Pickering emulsions (HIPPEs) were explored as fat replacers for 3D-printed meat products. HIPPEs with varying Lyc concentrations were formulated, and their encapsulation efficiency and antioxidant activity (DPPH and ABTS assays) were evaluated. The encapsulation efficiency of Lyc exceeded 90% for all samples.

Plant-based diets, genetic predisposition, and the risk of adult-onset asthma A prospective study.

Background: Emerging evidence has linked diets rich in plant-based foods with a lower risk of asthma, while the prospective evidence is limited. Therefore, we aimed to explore the association of plant-based diet quality with the risk of adult-onset asthma, and whether this association was modified by genetic predisposition.

Methods: A total of 163,689 asthma-free participants were enrolled in from the UK Biobank.

Factors influencing customers' switching intentions to new energy vehicles based on the push-pull mooring model using SEM-ANN approach in Shanxi, China.

This study applied the Push-Pull Mooring (PPM) theoretical framework to investigate how push, pull, and mooring factors influence sustainable transportation choices. The main objective is to explore which push, pull, and mooring factors significantly influence the intention to switch from conventional vehicles to NEVs. The study utilized the SEM-ANN approach based on a dataset of 822 participants collected in Shanxi, China.

Super-resolution femtosecond electron diffraction reveals electronic and nuclear dynamics at conical intersections.

Conical intersections play a pivotal role in excited-state quantum dynamics. Capturing transient molecular structures near conical intersections remains challenging due to the rapid timescales and subtle structural changes involved. We overcome this by combining the enhanced temporal resolution of mega-electron-volt ultrafast electron diffraction with a super-resolution real-space inversion algorithm, enabling visualization of nuclear and electronic motions at conical intersections with sub-angstrom resolution, surpassing the diffraction limit.

Leber's hereditary optic neuropathy-associated ND1 3733G>C mutation ameliorates the mitochondrial quality control and cellular homeostasis.

Leber's hereditary optic neuropathy (LHON) is a paradigm for mitochondrial retinopathy because of mitochondrial DNA (mtDNA) mutations. However, the mechanism underlying LHON-linked mtDNA mutations, especially their impact on mitochondrial and cellular integrity, is not well understood. Recently, the ND1 3733G>C (p.

Efficacy and Safety of Endoscopic Pancreatic Stenting for Traumatic Pancreatic Fistula.

Objective: Traumatic pancreatic fistula is challenging and complicated to manage. Our aim was to assess the efficacy of endoscopic pancreatic duct stenting in the treatment of traumatic pancreatic fistula.

Methods: Patients with pancreatic trauma treated by endoscopic pancreatic duct stenting at Jinling Hospital from May 2016 to October 2022 were enrolled.

Benefits and challenges of host depletion methods in profiling the upper and lower respiratory microbiome.

Metagenomic sequencing for respiratory pathogen detection faces two challenges: efficient host DNA depletion and the representativeness of upper respiratory samples for lower tract infections. In this study, we benchmarked seven host depletion methods, including a new method (F_ase), using bronchoalveolar lavage fluid (BALF), oropharyngeal swab (OP), and mock samples. All methods significantly increased microbial reads, species richness, genes richness, and genome coverage while reduced bacterial biomass, introduced contamination, and altered microbial abundance.

Comparative Analysis of Long-Term Measles Immune Response After Natural Infection and Routine Vaccination in China.

Given the significant impact of population immunity on the measles epidemic, understanding immunity differences among populations with varying immunity backgrounds is necessary for identifying immunity gaps and informing vaccination policies. In this study, we aimed to determine the distinct dynamics of vaccine-induced and naturally acquired antibodies, with specific focus on difference in vaccine-induced antibody responses across different birth cohorts. Based on two cohorts and one cross-sectional study conducted in Anhua County, Hunan Province, China, serum samples from children who followed China's routine measles vaccination schedule (i.

Characteristics and Changes of Cingulate Gyrus Function and Perfusion in Patients With Anti-N-Methyl-D-Aspartate Receptor Encephalitis.

Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is an autoimmune condition associated with neuropsychiatric and cognitive deficits. Changes in the cingulate cortex may be central to this disorder. This study investigates subregional alterations in the cingulate cortex of patients with anti-NMDAR encephalitis and their relationship to cognitive deficits using functional and perfusion imaging.

Photolysis of methyl nitrate (CH3ONO2) through the prism of ab initio simulations of transient-absorption pump-probe spectra.

The photolysis of methyl nitrate (CH3ONO2) UV-excited to the optically bright state is scrutinized by on-the-fly trajectory surface hopping simulations of dynamic observables and transient-absorption pump-probe (TA-PP) spectra. It is found that two major dissociation channels, CH3O + NO2 and CH3O + NO + O, are characterized by the two branches of the stimulated emission signal, which are clearly seen in the total experimentally detectable TA-PP signal. Correlations between the photolysis channels and their TA-PP signatures are established.

Mobile phone use characteristics, genetic predisposition and the risk of Parkinson's disease: a prospective cohort study.

We investigated the association between mobile phone use characteristics and Parkinson's disease (PD) risk and whether genetic predisposition modified these associations, utilising data from the UK Biobank. Participants using mobile phones for at least one hour weekly had a lower risk of PD compared to those using less than five minutes weekly (HR: 0.75, 95% CI: 0.

Relapses during treatment with monoclonal antibodies targeting B-cells in NMOSD.

Introduction: While sustained B-cell depletion has proven effective in maintaining a relapse-free state in patients with neuromyelitis optica spectrum disorder (NMOSD), B-cell-depleting therapies do not demonstrate efficacy in all cases. This study aims to investigate the risk factors associated with relapses during B-cell-targeting monoclonal antibody (mAb) treatment in patients with NMOSD.

Methods: We retrospectively analyzed baseline clinical characteristics, B-cell test results, and relapse data from NMOSD patients who received B-cell-targeting mAb treatment at four medical centers from July 2014 to September 2024.

[Mechanism of BNIP3-mediated mitophagy in m.3635G>A related Leber hereditary optic neuropathy].

Objective: To explore the mechanism of BNIP3-mediated mitophagy in m.3635G>A related Leber's hereditary optic neuropathy (LHON).

Methods: A trans-mitochondrial cybrid cell line derived from a Chinese LHON patient carrying the m.

Association between the quality of plant-based diets and risk of thyroid dysfunction.

: Plant-based diets are generally considered to be associated with a decreased risk of several chronic diseases. However, there is limited prospective evidence on the association between plant-based diet quality and thyroid dysfunction (TD) risk. Therefore, we aimed to explore the longitudinal associations between plant-based diets and TD (encompassing hypothyroidism and hyperthyroidism) risk.

Measles Population Immunity in Hunan, China: A Serological Assessment.

Background: Assessing the measles immunity profile is critical for developing effective nationwide or regionwide supplementary immunization activities (SIAs). This study aims to assess measles population immunity levels in China and investigate factors contributing to age-specific heterogeneities.

Methods: We conducted a cross-sectional population-based serological study in southern China between June and October 2021.

Roles of Serum NLRP3 Inflammasome and Associated Cytokines in Gout-Induced Kidney Injury.

Introduction: Gout, characterized by hyperuricemia and urate crystal deposition, is associated with systemic inflammatory complications, including kidney injury. This study aimed to investigate the role of serum nucleotide-binding oligomerization domain-like receptor 3 (NLRP3) inflammasome and associated cytokines (IL-1β and IL-18) in gout-related kidney injury (GRI).

Methods: A total of 279 gout patients (96 with renal injury and 183 without renal injury) and 100 healthy controls were included.