Application of engineered CRISPR/Cas12a variants with altered protospacer adjacent motif specificities for the detection of isoniazid resistance mutations in .

Unlabelled: Drug-resistant tuberculosis (TB) is a major global public health concern. Although isoniazid is currently considered one of the most effective first-line drugs for TB treatment, its efficacy is limited by the emergence of resistance. Therefore, it is imperative to develop new methods for detecting drug-resistant TB.

Heterogeneity-optimized method for predicting immune checkpoint blockade response.

Interpatient tumor heterogeneity manifests as multimodal distributions across genomic, transcriptomic, and microenvironmental profiles. This fundamentally violates the unimodal assumption of conventional machine learning models, impairing immune checkpoint blockade (ICB) response prediction. To resolve this limitation, we propose a heterogeneity-optimized framework that applies K-means clustering to stratify patients into biologically distinct hot-tumor and cold-tumor subgroups, demonstrating superiority over hierarchical/DBSCAN clustering.

Prediction Model and Assessment of Work-Related Musculoskeletal Disorders Among Nurses in Tertiary Hospitals in China: A Cross-Sectional Study.

Work-related musculoskeletal disorders (WMSDs) significantly impact the health and work efficiency of nurses. This study aimed to investigate factors influencing WMSD risk among nurses, including protective measures, physical activity, and biomechanical factors. This cross-sectional study surveyed 3500 nurses from tertiary hospitals to assess musculoskeletal disorder risk factors, yielding 3380 valid responses.

Transition Metal Tunes Molecular Self-Assembly in Living Cells for Controlling Cell-Cell Crosstalk.

This work reports a robust and versatile strategy for harnessing cell-compatible transition metal catalysts to tune peptide self-assembly precisely in living cells. The engineered cell-compatible targets cell membranes by chemically tagging membrane-affinity groups and functionalizing the surfaces of living mammalian cells. The anchored on the cell surface can then recognize the designed substrate and induce the self-assembly of peptides through olefin metathesis.

Efficacy and Safety of Femtosecond Laser-Assisted Cataract Surgery with the Catalys Platform in Patients with Type 2 Diabetes and Cataract: A Prospective Cohort Study.

Introduction: In recent years, the continuous rise in diabetes prevalence has led to a significant increase in the number of patients with diabetic cataracts. Femtosecond laser-assisted cataract surgery (FLACS), as an innovative technology in the field of cataract treatment, has demonstrated remarkable advantages over conventional phacoemulsification (CPS) in terms of surgical precision and efficiency. However, the unique ocular pathological changes in patients with diabetes (such as fragile lens capsules, posterior synechiae of the iris, and increased risk of postoperative inflammatory reactions) pose distinct challenges for cataract surgery.

The impact of game character identification on otome game players' mate selection criteria.

As a type of simulation love game, otome games provide women players with an immersive and sweet romantic experience, which may have a subtle impact on their mate selection criteria. In order to explore the impact of game character identification on the mate selection criteria of women gamers, a survey was conducted with 351 women otome game players. The study found that there was a pairwise positive correlation between the game character identification, self-presence, character attachment, and mate selection criteria of women players.

Incidence, Severity, and Pain Self-Efficacy of Low Back Pain in the Female Healthcare Professionals and Their Impact on Overall Quality of Life: A Multicenter Retrospective Study.

Female healthcare professionals are prone to experiencing high rates of non-specific low back pain (LBP), significantly impacting their performance and overall well-being. This multi-center, retrospective study aims to investigate the frequency and severity of non-specific LBP among female healthcare professionals. Additionally, it seeks to assess the relationship among patients' physical condition, self-belief attributes, and quality of life, providing a comprehensive understanding of this crucial occupational health concern.

Multi-courses of blinatumomab combined with reduced dose chemotherapy has been successfully used in chemo-intolerant middle to high-risk pediatric B-ALL patients with invasive fungal disease.

Invasive fungal disease (IFD) remains a challenging complication and a leading cause of death in the treatment of childhood acute leukemia (AL). Blinatumomab is a novel bispecific antibody targeting CD19, with excellent anti-tumor effects against B cell malignancies, including B cell acute lymphoblastic leukemia (B-ALL). Compared with standard chemotherapy, blinatumomab causes less immunosuppression.

Correlation between changes in haemorheology and the HIF-1/EPO pathway in children with pneumonia.

ObjectiveTo investigate the possible correlation between changes in haemorheology and the HIF-1/EPO pathway in children with pneumonia (MPP) to provide new ideas for the early clinical identification of hypercoagulability and reduce the incidence of thrombosis.MethodsA total of 205 children with newly diagnosed MPP were selected from our department and divided into a general MPP group (100 patients) and a severe MPP group (105 patients) according to diagnostic criteria. In addition, 30 healthy children were selected as the control group.

68 Ga-DOTA-IBA Uptake in Meningioma and Liver Metastasis of Breast Cancer.

A 41-year-old woman with bone and liver metastasis of breast cancer was enrolled in our 68 Ga-labeled DOTA-ibandronic acid ( 68 Ga-DOTA-IBA) PET/CT tumor clinical trial. The examination revealed increased uptake of 68 Ga-DOTA-IBA in the multiple bone metastases. Unexpectedly, 68 Ga-DOTA-IBA uptake in meningioma and liver metastasis were also noted.

Vγ9Vδ2 T cells expanded with vitamin C combined with HMBPP inhibit intracellular growth.

Background: presents a significant global health threat, and the existing treatments have notable limitations. Vγ9Vδ2 T cells activated by HMBPP can inhibit the growth of intracellular Mtb. Additionally, vitamin C (VC) promotes the differentiation and proliferation of related T cells.

Programming two-component peptide self-assembly by tuning the hydrophobic linker.

Molecular self-assembly enables the formation of intricate networks through non-covalent interactions, serving as a key strategy for constructing structures ranging from molecules to macroscopic forms. While zero-dimensional and one-dimensional nanostructures have been widely achieved, two-dimensional nanostrip structures present unique advantages in biomedical and other applications due to their high surface area and potential for functionalization. However, their efficient design and precise regulation remain challenging.

Comparison of Ga-DOTATATE and F-FDG PET/CT for tumor staging and primary tumor volume delineation in patients with nasopharyngeal carcinoma.

This study evaluated the clinical utility of Ga-DOTATATE PET/CT compared to F-FDG PET/CT for tumor staging and the delineation of primary tumor volume in patients with non-keratinizing nasopharyngeal carcinoma (NPC). Forty-two individuals with pathologically confirmed non-keratinizing NPC were recruited. This study compared the detection rates of primary and metastatic tumors and the accuracy of tumor staging using two PET/CT modalities.

Abnormally high expression of CHI3L1 in peripheral blood mononuclear cells and serum and their potential diagnosis and prediction from lymphoma patients.

Aim: This study aimed to investigate the expression of CHI3L1 in aggressive lymphomas and assess its potential as a diagnostic and prognostic biomarker.

Methodology: This study investigates the expression of CHI3L1 protein in the peripheral blood of patients with aggressive lymphoma and healthy controls using enzyme-linked immunosorbent assay (ELISA). The prognostic significance of CHI3L1 was assessed through Cox regression and Kaplan-Meier survival analyses.

Cecropin A-Derived Peptide for the Treatment of Osteomyelitis by Inhibiting the Growth of Multidrug-Resistant Bacteria and Eliminating Inflammation.

Osteomyelitis poses substantial therapeutic challenges due to the prevalence of multidrug-resistant bacterial infections and associated inflammation. Current treatment regimens often rely on a combination of corticosteroids and antibiotics, which can lead to complications and impede effective bacterial clearance. In this study, we present CADP-10, a Cecropin A-derived peptide, designed to target methicillin-resistant (MRSA) and multidrug-resistant (MRE), while simultaneously addressing inflammatory responses.

Photothermal-enhanced ROS storm by hyaluronic acid-conjugated nanocatalysts to amplify tumor-specific photo-chemodynamic therapy and immune response.

Integrating photodynamic therapy (PDT) and chemodynamic therapy (CDT) shows promising potential in tumor treatment. Nevertheless, the lack of specific tumor targeting, serious photobleaching, and poor photothermal effect of photosensitizers, the intracellular low Fenton reaction efficiency, and glutathione (GSH)-elicited reactive oxygen species (ROS) depletion profoundly restrict ROS-mediated cancer therapy. To enhance ROS generation with the assistance of photothermal therapy (PTT), the hyaluronic acid (HA)-decorated Fe-MIL-88B (MIL) nanocatalysts were fabricated for tumor-targeted delivery of photosensitizer IR820.

[Protein C activator derived from snake venom protects human umbilical vein endothelial cells against hypoxia-reoxygenation injury by suppressing ROS upregulating HIF-1α and BNIP3].

Objectives: To investigate the antioxidative mechanism of snake venom-derived protein C activator (PCA) in mitigating vascular endothelial cell injury.

Methods: Human umbilical vein endothelial cells (HUVECs) were cultured in DMEM containing 1.0 g/L D-glucose and exposed to hypoxia (1% O) for 6 h followed by reoxygenation for 2 h to establish a cell model of oxygen-glucose deprivation/reoxygenation (OGD/R).

The mouse resource at National Resource Center for Mutant Mice of China.

Mouse models serve as the most important laboratory resource for both biomedical research and preclinical study of drug development. National Resource Center of Mutant Mice (NRCMM) of China was initiated in 2001 and became one of the 31 members of National Science and Technology Resource Sharing Platform in 2019. Currently, NRCMM is co-managed by Model Animal Research Center of Nanjing University and Gempharmatech (GPT, a Shanghai Exchange enlisted public company).

An analysis of the clinical value of CHI3L1 as a biomarker of multiple myeloma progression.

Background: The aim of this study was to systematically assess the relationship between Chitinase 3-like protein-1 (CHI3L1) protein and disease progression in multiple myeloma (MM) and to explore its potential clinical value as a biomarker to provide a basis for optimizing treatment strategies.

Methods: 136 patients with MM were divided into two groups according to the efficacy: group 1-95 patients with non-progressing MM; group 2-41 patients with progressing MM. The concentration of CHI3L1 in the serum of the patients was determined by enzyme-linked immunosorbent assay (ELISA).

N-Acetyltransferase 10 Promotes Gastric Cancer Progression by Mediating the N4-Acetylcytidine Modification of Lactate Dehydrogenase A.

The N4-acetylcytidine (ac4C) modification, which is catalyzed by NAT10, represents a significant posttranscriptional modification of mRNA in multiple cancers. However, the significance of this modification in gastric cancer (GC) progression remains unclear. To evaluate the potential of differential NAT10 expression in GC, RT-qPCR and western blot were employed.

Carotid artery hemodynamics and geometry as predictors of cerebral small vessel disease: insights from 4D flow imaging.

Background And Purpose: Cerebral small vessel disease (cSVD) is a common condition with complex pathophysiology, resulting in poor clinical outcomes. This study uses four-dimensional flow (4D flow) imaging to examine how carotid artery hemodynamics and geometry impact cSVD development.

Methods: Combining ultrasound, 4D flow, and three-dimensional time-of-flight magnetic resonance angiography (3D-TOF MRA), we analyzed and measured internal carotid artery (ICA) hemodynamics and geometric parameters.

SIRT5: a potential target for discovering bioactive natural products.

Silent information regulator 5 (SIRT5) is the fifth member of the sirtuin family, which is mainly expressed in mitochondrial matrix. SIRT5 plays a key role in metabolism and antioxidant responses, and is an important regulator for maintaining intracellular homeostasis. Given its involvement in multiple cellular processes, dysregulation of SIRT5 activity is associated with a variety of diseases.

Comparison of the effect of ranibizumab in retinal vein occlusion and macular edema with different optical coherence tomographic patterns.

Aim: To explore the morphological and functional parameters to evaluate the effectiveness of intravitreal injections of ranibizumab (IVR) in treating macular edema (ME) secondary to retinal vein occlusion (RVO).

Methods: This retrospective study involved 65 RVO patients (65 eyes) who received IVR and were followed-up for more than 3mo. ME was categorized into cystoid macular edema (CME), diffuse retinal thickening (DRT), and serous retinal detachment (SRD) according to optical coherence tomography (OCT) images.