Dual-Mode Method for the Sensitive Detection of β-Secretase (BACE1) Based on Surface-Enhanced Raman Scattering and Dark-Field Microscopy.

β-Secretase (BACE1), a key enzyme to producing neurotoxic β-amyloid, is a potential biomarker of Alzheimer's disease (AD). Developing a sensitive and efficient detection method for BACE1 activity is significant for AD progression evaluation. Due to the poor cleavage efficiency and acidic working conditions of BACE1, developing probes with high stability and strong signals is challenging for its detection.

The effects of formula milk on development, nutrient absorption, and immune characteristics of piglets.

Background: Breast milk is widely regarded as the optimal nourishment for infants. However, due to physiological or psychosocial factors, many infants do not receive adequate breastfeeding. So, the formula milk is proposed as a substitute of breast milk, and an increasing number of infants are being formula-fed.

The Association of Proteinuria Target Achievement Timing and Stability with Adverse Kidney Outcomes Among Patients with IgA Nephropathy: A Cohort Study.

Introduction: Current KDIGO guidelines for IgA nephropathy (IgAN) recommend proteinuria should be maintained at <0.5 g/day. In this study, we aim to evaluate the association of proteinuria target achievement timing and stability with adverse kidney outcomes.

Targeted Delivery of Exosome-Derived miRNA-185-5p Inhibitor via Liposomes Alleviates Apoptosis and Cuproptosis in Dilated Cardiomyopathy.

Purpose: Dilated cardiomyopathy (DCM) is a prevalent form of heart failure with limited therapeutic options. This study explores a novel treatment strategy involving the delivery of exosome-derived miRNA-185-5p inhibitors encapsulated in liposomes, aiming to target cardiac tissue and alleviate myocardial apoptosis and cuproptosis in DCM.

Methods: The miRNA-185-5p inhibitor, identified in our previous study and extracted from exosomes, was encapsulated in liposomes functionalized with a cardiac-targeting peptide.

1,3-Dichloro-2-propanol Causes Lipid Droplet Accumulation by Inhibiting Autophagy via REV-ERBα in Hepatocytes.

1,3-Dichloro-2-propanol (1,3-DCP), a food processing contaminant, induces hepatic lipid accumulation through unclear mechanisms. The circadian clock system plays a central role in regulating cellular metabolism. This study investigated the exact mechanisms underlying 1,3-DCP-induced lipid droplet (LD) accumulation and the potential regulatory role of the circadian clock.

Accelerating the exploration of material space for hydride double perovskite superconductors under ambient pressure through machine learning.

In the field of hydride superconductors, a great challenge is to achieve superconducting states under ambient pressure conditions rather than the extreme high-pressure environments that have been required in experiments. Achieving this goal is crucial for advancing the practical applications of high-temperature superconducting materials. We discover a family of compounds (hydride double perovskite superconductors with space group Fm3̄m and chemical formula A2MM'H6) to achieves this goal.

Clinical Characteristics and Molecular Aetiology of Cytochrome P450 Oxidoreductase Deficiency Diagnosed in 46,XX Patients.

P450 oxidoreductase deficiency (PORD) affects cytochrome enzyme activities, causing various symptoms, such as adrenal insufficiency, disorders of sex development and skeletal malformations. This study aims to elucidate the clinical manifestations, genotype characteristics, diagnosis and management of 46,XX karyotype patients with PORD in China. A retrospective study included twelve 46,XX PORD patients in a Chinese tertiary medical center from 2004 to 2024.

Polysaccharide from Aloe vera gel improves intestinal stem cells dysfunction to alleviate intestinal barrier damage via 5-HT.

Metabolites of dietary fibers which consist of non-digestible carbohydrates (usually as plant-based polysaccharides) exert many benefits on gastrointestinal health. Previous studies proved that polysaccharides can change intestinal metabolic pathways, increase metabolite levels and alleviate intestinal inflammation. Our previous studies have shown that polysaccharide from Aloe vera gel (AGP) alleviated intestinal barrier dysfunction.

Regulating triacylglycerol cycling for high-efficiency production of polyunsaturated fatty acids and derivatives.

Lipid degradation is generally considered an antagonistic pathway to lipid synthesis, so this pathway is often removed to improve lipid production. In this study, triacylglycerol (TAG) cycling formed by lipid degradation is found to be crucial for long-chain polyunsaturated fatty acid (PUFA) biosynthesis; this result contradicts the notion that lipid degradation is a useless process. Specifically, we demonstrate that TAG cycling promoting PUFA biosynthesis occurred in Yarrowia lipolytica and Mortierella alpina via the desaturase/elongase pathway but not in Schizochytrium sp.

Mechanism of interaction between key active ingredients and myosin in tea-stewed beef: Improvement of myosin's structural and functional characteristics.

This study analyzed the tea polyphenol components in four types of tea-stewed beef, identifying five key active compounds: epicatechin (EC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), and caffeine (CAF). Oxidative stability, structural changes, and antioxidant activity of complexes formed between these compounds and bovine myosin were assessed. Molecular docking and dynamic simulations were employed to investigate the interaction mechanisms.

The clinical diversity and molecular etiology in 46, XY disorders of sex development patients without uterus.

Background: Disorders of sexual development (DSDs) are a group of rare conditions with a discordance of chromosomal, gonadal, or phenotypic features of the internal and/or external genitalia, which accounts for 0.5% of the population. The precise diagnosis of 46, XY DSDs without uterus is often obscure because of the similar clinical manifestations.

Pea protein isolate-wheat bran arabinoxylan glycated complex improves the physical stability and bioaccessibility of O/W emulsion.

The developing of green plant based protein as excellent emulsifier to prepare stable O/W emulsion has become crucial in food processing. This study investigated the emulsification property enhancing of pea protein isolate (PPI) through glycation with wheat bran arabinoxylan (WBAX) to form a covalent complex (PPI-WBAX), the stability and the application in encapsulating β-carotene of the prepared PPI-WBAX emulsion. Results showed that glycation improved the emulsification property of PPI as evidenced by the increased emulsifying activity index (6.

Ratiometric fluorescence sensor for Escherichia coli detection using fluorescein isothiocyanate-labeled metal-organic frameworks.

A ratiometric fluorescence sensor for detecting Escherichia coli (E. coli) was fabricated based on the fluorescein isothiocyanate (FITC)-labeled zirconium (Zr)-tetraphenylporphyrin tetrasulfonic acid (TPPS) hydrate metal-organic frameworks (ZTMs@FITC). The ZTMs have strong red fluorescence emission at 683 nm, which can be quenched by Cu.

Enhanced room temperature ammonia gas sensing based on a multichannel PSS-functionalized graphene/PANI network.

Disordered polymerization of polymers widens the polymerization degree distribution, which leads to uncontrollable thickness and significantly weakens their sensing performance. Herein, poly(sodium -styrenesulfonate)-functionalized reduced graphene oxide (PSS-rGO) with multichannel chain structures coated with thin polyaniline layer (PSS-rGO/PANI) nanocomposites was synthesized a facile interfacial polymerization route. The morphology and microstructure of the PSS-rGO/PANI nanocomposites were characterized using Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM).

Polysaccharides as natural enhancers for meat quality, preservation, and protein functionality: A comprehensive review.

Recent research focuses on developing meat products with health-promoting properties to reduce disease risk, particularly using natural polysaccharides due to their antioxidant and antibacterial effects. These polysaccharides, sourced from various materials, act through diverse structural mechanisms, inhibiting pathogen growth, enhancing oxidative stability, and improving meat flavor. This study highlights the role of meat proteins in achieving the Sustainable Development Goals (SDGs) and their importance in enhancing processed meat quality.

To Post or not to Post? That is the Question. Relationship between Selfie Behavior and Cosmetic Surgery Attitude among Chinese Young Adult Women: Mediation of Negative Physical Self.

Background: Recently, young women have become the primary drivers of the increasing demand for cosmetic surgery. Previous studies have indicated a significant positive correlation between selfies and considering cosmetic surgery. However, limited research has explored the relationship between different types of selfies (i.

Identification of novel variants and candidate genes in women with 46,XX complete gonadal dysgenesis.

Background: 46,XX complete gonadal dysgenesis (46,XX-CGD) is a rare disorder of sexual development (DSD) characterized by primary amenorrhea and a lack of spontaneous pubertal development in individuals with a 46,XX karyotype despite the presence of female internal and external genitalia due to failure of bilateral ovarian development. The condition is genetically heterogeneous, and in most cases, its etiology is unknown. Determining the genetic cause would provide insights into potential targets for genetic diagnosis and counseling.

Synergistic bactericidal effect of antimicrobial peptides and copper sulfide-loaded zeolitic imidazolate framework-8 nanoparticles with photothermal therapy.

Antimicrobial resistance (AMR) has emerged as a significant threat to human health. Antimicrobial peptides (AMPs) have proven to be an effective strategy against antibiotic-resistant bacteria, given their capacity to swiftly disrupt microorganism membranes and alter cell morphology. A common limitation, however, lies in the inherent toxicity of many AMPs and their vulnerability to protease degradation within the body.

Reproductive and transcriptome characteristics of endometrium at the window of im-plantation in patients after fertility-sparing treatment of endometrial intraepithelial neoplasm or cancer.

Background: Progestin therapy is an option for patients with endometrial carcinoma (EC) or endometrial intraepithelial neoplasm (EIN) who fit specific criteria of fertility-sparing treatment. However, the implantation rate remains low among females receiving in vitro fertilization (IVF) even after the complete reversal of endometrial lesions.

Methods: Here, ten patients with EC/EIN achieved complete regression (CR) in histology.

Sensitive colorimetric detection of Escherichia coli in milk using Au@Ag core-shell nanoparticles.

Escherichia coli (E. coli) is a prevalent pathogen that is frequently associated with the foodborne illness. It causes various infections and poses a significant threat to human health.

β-Glucan Relieves Symptoms of Colitis via PPARα-Mediated Intestinal Stem Cell Proliferation.

Intestinal stem cells (ISCs) are necessary to maintain intestinal renewal. Here, we found that the β-glucan (HBG) alleviated pathological symptoms and promoted the proliferation of intestinal stem cells in colitis mice. Notably, metabolomics studies showed that docosahexaenoic acid (DHA) was significantly increased by the HBG treatment.

Optimizing multicopy chromosomal integration for stable high-performing strains.

The copy number of genes in chromosomes can be modified by chromosomal integration to construct efficient microbial cell factories but the resulting genetic systems are prone to failure or instability from triggering homologous recombination in repetitive DNA sequences. Finding the optimal copy number of each gene in a pathway is also time and labor intensive. To overcome these challenges, we applied a multiple nonrepetitive coding sequence calculator that generates sets of coding DNA sequence (CDS) variants.