Functional divergence of G protein γ subunits drives plant vigor and improvement through the CPK28-PIP1;2 pathway in tomato.

Maintaining robust plant vigor is essential for sustaining crop productivity, yet the precise roles and molecular underpinnings of G protein γ subunits in this process remain elusive. This study reveals that GGC1 is under selection during tomato domestication, and its mutants exhibit enhanced plant vigor, characterized by superior growth, increased yield, and improved fruit quality. In contrast, triple mutants gga1/ggb1/ggb2 display severely compromised vigor resembling slgb1 mutants lacking the Gβ subunit.

Dendritic Mesoporous SiO-Based SERS-Nanozyme-Mediated Immunochromatographic Detection of Foodborne Viruses with Enhanced Sensitivity across Multiple Scenarios.

Detecting low-concentration foodborne viruses in complex samples has long posed a great challenge. Here, we propose a colorimetric enhancement-surface-enhanced Raman scattering (SERS) quantitative dual-mode immunochromatographic assay (ICA), characterized by high flexibility, sensitivity, and stability, which can rapidly and accurately detect viruses in various environments, including field, home, and clinical laboratory settings. A multifunctional SERS nanozyme tag (DSAIA) is customized using dendritic mesoporous SiO as the core, which is densely loaded with AuIr catalytic particles and coated with a layer of highly active 35 nm Au nanoparticles on the exterior, thereby simultaneously achieving monodispersity, strong peroxidase activity, and a high density of efficient SERS hotspots.

Electroacupuncture Pretreatment Alleviates Myocardial Ischemia-Reperfusion Injury by Inhibiting Engulfment by Microglia in the Lateral Hypothalamus.

Aim: The occurrence of myocardial ischemia-reperfusion injury (MIRI) is accompanied by neuroinflammatory reactions and is closely related to the overactivation of microglia. Currently, effective clinical strategies to prevent MIRI are unclear. This study investigated potential therapeutic targets and the mechanisms of electroacupuncture pretreatment (EA-pre) for MIRI.

Selection of geographical populations suitable for artificial breeding of the Northeast China Brown Frog (Rana dybowskii).

Amphibians, as a group greatly disturbed by human activities, are at increased risk of extinction. Rana dybowskii is an anuran species with both ecological and economic significance. Due to environmental changes and human overexploitation, it has been classified as Near-Threatened.

A Two-Sample Mendelian Randomization Research Examining the Causal Association Between Ischemic Stroke and Obstructive Sleep Apnea.

Background: Although prior observational research has indicated a potential association between ischemic stroke (IS) and obstructive sleep apnea (OSA), it is still unclear how the two conditions are causally related. The purpose of this study is to evaluate the causal association between IS, including large artery stroke (LAS), cardioembolic stroke (CES), small vessel stroke (SVS), and OSA by the Mendelian randomization (MR) approach.

Methods: To avoid bias caused by heterogeneity among populations with different genetic backgrounds, this study selected only databases related to the European population for analysis.

Galla Chinensis as an antiviral agent: Targeting Micropterus salmoides rhabdovirus infection.

Being a major pathogen affecting the health of largemouth bass (Micropterus salmoides) aquaculture, Micropterus salmoides rhabdovirus (MSRV) causes significant economic losses. Galla chinensis, a traditional Chinese medicinal herb, possesses diverse biological activities, including antiviral properties. This study evaluated the role of Galla chinensis in MSRV infection in vitro and investigated its mechanism of action.

Maximal inspiratory diaphragmatic ultrasound predicts postoperative pulmonary complications after upper abdominal surgery.

Background: Postoperative pulmonary complications (PPCs) after major upper abdominal surgery are an important cause of morbidity and mortality. However, existing preoperative risk models inadequately address perioperative factors. Although diaphragmatic ultrasonography offers real-time assessment of respiratory muscle function, its predictive utility for PPCs remains underexplored.

Updated insights on memory disorder associated risk of medication: A real-world pharmacovigilance analysis.

Objective: This study aims to use the Food and Drug Administration Adverse Event Reporting System (FAERS) to identify medications linked to memory disorders and to offer updated insights into medication-induced memory disorders.

Methods: We analyzed adverse event (AE) reports related to memory disorders in the FAERS database from 2004 to 2023 and compiled a list of potential medications along with their associated AEs. The disproportionality analysis was employed to assess the potential associations between medications and memory disorder events.

Soy Isoflavone Supplementation in Sow Diet Enhances Antioxidant Status and Promotes Intestinal Health of Newborn Piglets.

This study aimed to explore the effects of dietary supplementation with soy isoflavones (SI) in the later stages of pregnancy on the antioxidant capacity of sows and intestinal health of newborn piglets. Forty sows with similar body weights and parity (average of 1-2 parity) were randomly divided into two groups ( = 20): the control group and SI group (dose: 100 mg/kg of feed). Feeding was started on day 85 of gestation and continued until farrowing.

Age-Related Mitochondrial Alterations Contribute to Myocardial Responses During Sepsis.

Sepsis-induced myocardial injury is age-related and leads to increased mortality. Considering the importance of mitochondrial dysfunction in cardiac impairment, we aimed to investigate whether aging exacerbates the cardiac mitochondrial metabolic response to inflammation, thus leading to increased cardiac dysfunction in the elderly. Cecal ligation and puncture (CLP) was conducted in young adult (12-18 weeks) and aged (19-21 months) male C57BL/6 mice.

Phyhip-Targeted Delivery of bFGF Platform with Dual Modulation for Enhancing Neurovascular Repair in Traumatic Brain Injury.

Traumatic brain injury (TBI) remains a critical neurosurgical challenge with limited therapeutic options. While basic fibroblast growth factor (bFGF) demonstrates neuroprotective and angiogenic potential, its clinical translation is hindered by nonspecific biodistribution and poor blood-brain barrier (BBB) penetration. In the present study, a brain-targeted recombinant protein (AcuP-bFGF) is developed by fusing bFGF with an acute peptide (SLYGSSRHTAPISF, named as AcuP), which enables Phyhip-mediated active transport across the compromised BBB while preserving the bioactivity of bFGF and prolonging its half-life in vivo.

Baseline expression of c-Myc defines the tissue specificity of oncogenic K-Ras.

is among the most frequently mutated oncogenes in cancer. Yet, mutations in are common only in tumors originating from a subset of tissues. It is critical to understand the molecular mechanisms underlying this oncogene tissue specificity.

Association between serum corin in the second trimester and hypertensive disorders of pregnancy: a mendelian randomization study.

Background: Serum corin has been associated with hypertensive disorders of pregnancy (HDP) in clinical studies but lacks causal evidence. This study aimed to systemically examine the associations of serum corin and its coding gene polymorphisms with HDP, as well as the underlying causality among Chinese pregnant women.

Methods: A nested case-control study comprising 403 cases of HDP and 402 age- and parity-matched healthy controls was designed.

Genome-wide identification and characterization of family gene in cotton and their potential role in salt stress tolerance.

Heat shock proteins 90 (HSP90s) are conserved molecular chaperones essential for cellular homeostasis under abiotic stress. While several studies have been reported to elucidate the role of HSP90s in heat stress responses in cotton, their involvement in salt stress remains underexplored. L.

Effect of electroacupuncture on anxiety in patients with obesity: a protocol for a randomised, blinded, sham-controlled trial.

Introduction: Obesity is a growing public health issue worldwide, and anxiety is a major psychological disorder associated with obesity. Electroacupuncture (EA) has been proven to be a feasible treatment modality for obesity and anxiety in clinical practice. However, data on the effectiveness of EA for anxiety patients with obesity are lacking.

Sex-Specific Differences in LPS-Induced Rapid Myocardial Dysfunction.

Cardiac dysfunction is a severe complication of sepsis that significantly increases mortality in affected patients. Previous studies have shown better myocardial responses with preserved cardiac function in female animals compared to males following lipopolysaccharide (LPS)-induced sepsis. Our published findings have revealed that females exhibited less cardiac dysfunction than males when exposed to equivalent doses of tumor necrosis factor (TNF)α, which is markedly elevated in both heart tissue and serum following LPS.

Prevalence of foodborne parasitic infections in market-sold aquatic products and high-risk populations in Shanghai, China: surveillance between 2022 and 2024.

Background: Substantial achievements have been made in preventing and treating human parasitic diseases in China over the past six decades. However, with the recent progression of economic globalization and food diversification, foodborne parasitic diseases have become a significant public health challenge. Here, we investigated and analyzed the characteristics of foodborne parasitic infections arising from market-sold aquatic food products and in key populations.

Orphan Nuclear Receptors TR2 and TR4 in Erythropoiesis: From Mechanisms to Therapies.

Testicular orphan receptors TR2 and TR4 serve as central regulators of erythropoiesis, orchestrating the entire continuum of erythroid progenitor cell proliferation, differentiation, and maturation. As core components of the direct repeat erythroid determinant (DRED) complex, they activate erythroid-specific transcriptional programs to dynamically control the spatiotemporal expression of globin genes. These nuclear receptors not only engage in functional interactions with key erythroid transcription factors GATA1 and KLF1 to coregulate erythroid differentiation and maturation but also recruit epigenetic modifier complexes such as DNMT1 and LSD1 to modulate chromatin states dynamically.

Efficacy and safety of PEGylated uricase in healthy adults and patients with hyperuricaemia: A phase I, randomized, double-blind, dose-escalation, placebo-controlled trial.

Aims: This Phase I trial aimed to evaluate the tolerability, pharmacokinetics, pharmacodynamics (PK/PD), and immunogenicity of PEGylated uricase (PEG-UOX) in healthy adults and patients with hyperuricaemia.

Methods: A total of 48 subjects-24 healthy adults and 24 patients with hyperuricaemia-were enrolled in a randomized, double-blind, placebo-controlled, dose-escalation study. Participants received a single intravenous dose of PEG-UOX ranging from 0.

Hyperglycemia induces an immunosuppressive microenvironment in colorectal cancer liver metastases by recruiting peripheral blood monocytes through the CCL3-CCR1 axis.

Background: The treatment of colorectal cancer liver metastasis (CRLM), a leading cause of mortality in patients with colorectal cancer, is complicated by type 2 diabetes (T2D). This study aimed to investigate the CRLM immune microenvironment in the context of T2D and identify potential therapeutic targets.

Methods: A hyperglycemic CRLM mouse model was established.

Comet Fragment-Ion Indexing for Enhanced Peptide Sequencing.

Fragment ion indexing has significantly improved the efficiency of proteomics database search tools. This work implements fragment ion indexing in Comet, a widely used, open-source search engine. We demonstrate that this enhancement maintains scoring and identification accuracy while substantially increasing peptide spectral matching speeds across multiple applications, including open modification searches, immunopeptidomics, and real-time searches.

From virtual to reality: application of a novel 3D printing hollow model for early-stage lung cancer in the clinical teaching of thoracoscopic sublobar resection.

Background: The integration of medical-engineering interdisciplinary technology has transformed clinical skills and anatomical knowledge teaching. Three-dimensional printing (3DP), an innovative tool, shows promise in enhancing surgical training and anatomical understanding. This study evaluates the educational efficacy of a 3DP lung cancer model optimized for surgery in teaching thoracoscopic sublobar resection.

Expert consensus on management of instrument separation in root canal therapy.

Instrument separation is a critical complication during root canal therapy, impacting treatment success and long-term tooth preservation. The etiology of instrument separation is multifactorial, involving the intricate anatomy of the root canal system, instrument-related factors, and instrumentation techniques. Instrument separation can hinder thorough cleaning, shaping, and obturation of the root canal, posing challenges to successful treatment outcomes.

The association between smoking and the occurrence of hyperuricemia: A retrospective cohort study.

Introduction: A retrospective cohort study was conducted to study the association between smoking and hyperuricemia (HUA).

Methods: By collecting and analyzing clinical data of 3196 patients with undiagnosed HUA at baseline in Dalian Municipal Central Hospital of China between 1 January 2010 and 1 January 2021, patients were grouped according to baseline smoking status and smoking index (the number of cigarettes smoked per day × number of years of smoking). Cox regression analysis was used to perform univariable and multivariable analyses of factors that may influence the occurrence of HUA.