An electrochemical immunosensor based on HFABP for the early detection of drug-induced cardiac toxicity from traditional Chinese medicines.

An electrochemical immunosensor was successfully developed for evaluating traditional Chinese medicine (TCM)-induced cardiac toxicity by immobilizing heart-type fatty acid-binding protein (HFABP)-specific antibodies on an electrode surface modified with chitosan (CS)-functionalized metal-organic framework ZIF-8 and gold nanoparticles (AuNPs). Leveraging the excellent biocompatibility of AuNPs, the excellent stability and high surface area of ZIF-8, along with the strong specific recognition capability of HFABP antibodies, the sensor demonstrated superior performance. Under optimized experimental conditions, it achieved an ultra-low detection limit of 7.

Stereo-cell: Spatial enhanced-resolution single-cell sequencing with high-density DNA nanoball-patterned arrays.

Single-cell sequencing technologies have advanced our understanding of cellular heterogeneity and biological complexity. However, existing methods face limitations in throughput, capture uniformity, cell size flexibility, and technical extensibility. We present Stereo-cell, a spatial enhanced-resolution single-cell sequencing platform based on high-density DNA nanoball (DNB)-patterned arrays, which enables scalable and unbiased cell capture at a wide input range and supports high-fidelity transcriptome profiling.

extracellular vesicle-driven oxygen-releasing photothermal hydrogel reprograms macrophages and promotes angiogenesis to accelerate diabetic wound healing.

Chronic wound healing remains clinically challenging due to insufficient angiogenesis coupled with persistent inflammatory microenvironments. Macrophage M2 polarization plays a pivotal role in resolving inflammation and promoting angiogenesis. Capitalizing on scalability and translational advantages, extracellular vesicles derived from (-EVs) were employed to activate this mechanism.

SIRT6 Lysine-Demyristoylates ATF2 to Ameliorate Vascular Injury via PRKCD/VE-Cadherin Pathway Regulating Vascular Endothelial Barrier.

Cardiovascular diseases (CVDs) progression is significantly modulated by epigenetic mechanisms, particularly through Sirtuin 6 (SIRT6), a key NAD⁺-dependent deacetylase in the sirtuin family. Though essential for cardiovascular homeostasis, the effects of SIRT6-mediated lysine myristoylation on CVDs progression remain largely unexplored due to detection limitations. This study developes an innovative lysine-myristoylated peptide enrichment technique, identifying mutant SIRT6 (H133Y) with high myristoyl affinity but deficient demyristoylase activity.

Small-period DUV plasmonic Al nanohole arrays fabricated by wet colloidal template/substrate and the interface and hole size-dependent E-field enhancement effect.

The fabrication of a uniform small-period Al nanohole array with pronounced deep-ultraviolet (DUV) plasmon is highly valuable in enhancing the intrinsic fluorescence of biochemical molecules for label-free detection. Since metal nanohole arrays have distinct optical transmission properties, it is often questioned whether the maximum electric field (E-field) enhancement effect coincides with the transmission peaks or transmission troughs. We efficiently fabricated uniform DUV plasmonic Al nanohole arrays with small periods by using a wet colloidal template/substrate in a facile and low-cost way.

Ruxolitinib, a novel JAK/STAT-targeted therapy through restoring mucosal homeostasis for chronic rhinosinusitis with nasal polyps.

Background: The Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway plays a crucial role in regulating immune function and inflammatory responses. Dysregulation of this pathway has been implicated in numerous disorders, including chronic rhinosinusitis with nasal polyps (CRSwNP). However, the potential therapeutic efficacy of targeted JAK inhibition in CRSwNP remains underexplored and requires further investigation.

Prevalence, correlates, functional impairment and mental health service gap of anxiety disorders among elderly population in China.

Aims: To report the prevalence and correlates of anxiety disorders among the elderly population in China; and to describe functional impairment and mental health service utilization due to anxiety disorders.

Methods: Data were derived from the Chinese Mental Health Survey. Weighted prevalence rates of various anxiety disorders were calculated.

SpaSEG: unsupervised deep learning for multi-task analysis of spatially resolved transcriptomics.

Spatially resolved transcriptomics (SRT) for characterizing spatial cellular heterogeneities in tissue environments requires systematic analytical approaches to elucidate gene expression variations within their physiological context. Here, we introduce SpaSEG, an unsupervised deep learning model utilizing convolutional neural networks for multiple SRT analysis tasks. Extensive evaluations across diverse SRT datasets generated by various platforms demonstrate SpaSEG's superior robustness and efficiency compared to existing methods.

Multi-Omics Analysis of Gut Microbiota and Sperm Quality in Tibetan Breeding Boars.

Background/objectives: Reproductive efficiency in breeding boars critically impacts swine industry productivity, with sperm quality being multifactorially regulated by gut microbiota. This study aimed to elucidate the microbiota-metabolite interactions underlying sperm quality differences in Tibetan boars.

Methods: Integrated 16S rRNA sequencing and untargeted metabolomics were performed on fecal and semen samples from eight healthy Tibetan boars (31-33 months old), stratified into low-semen (CJ) and high-semen utilization (HJ) groups.

Enhanced marine biological pump as a trigger for the onset of the late Paleozoic ice age.

The mid-Tournaisian carbon isotope excursion (TICE) represents the largest positive carbon isotope excursion in the late Paleozoic, coinciding with the onset of the late Paleozoic ice age (LPIA). Here, to investigate changes in the marine biological pump during the TICE, we measured barium isotopes (δBa) in two marine limestone sections in the Antler foreland basin (USA). We found the largest positive δBa shifts recorded in geological history, indicating increased marine export productivity in the Antler foreland basin, followed by the productivity-driven expansion of anoxia.

BDNF as a Key Mediator in Eosinophilic Inflammation in CRSwNP: Insights From Transcriptomic and Functional Analysis.

Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a heterogeneous inflammatory disorder characterized by eosinophil-driven pathology. While neurotrophins, particularly brain-derived neurotrophic factor (BDNF), have been implicated in eosinophilic inflammation in allergic conditions, their involvement in CRSwNP remains undefined.

Methods: Bulk-RNA sequencing of 23 nasal mucosa samples and ELISA quantification of 95 nasal secretions were conducted to evaluate BDNF expression across CRSwNP endotypes and its correlation with eosinophil infiltration and clinical severity.

Deciphering gene regulatory programs in mouse embryonic skin through single-cell multiomics analysis.

Background: Cell type-specific transcriptional heterogeneity in embryonic mouse skin is well-documented, but few studies have investigated the regulatory mechanisms. Here, we present high-throughput single-cell chromatin accessibility and transcriptome sequencing (HT-scCAT-seq), a method that simultaneously profiles transcriptome and chromatin accessibility. We utilized HT-scCAT-seq to dissect the gene regulatory mechanism governing epidermal stratification, periderm terminal differentiation, and fibroblast specification.

Construction of rigid polylactic acid/soft polytetrafluoroethylene dual nanofibers in poly(butylene adipate-co-terephthalate) to fabrication of PBAT microcellular foams with superior compression strength, anti-shrinkage and resilience.

Polybutylene adipate-co-terephthalate (PBAT) is attractive as a biodegradable polymer foam owing to its light weight, resilience, high flexibility and low price. However, modifying PBAT foam to improve its strength and reduce its shrinkage while maintaining its resilience has been a challenge. In this study, we used in situ fibrillation to incorporate a dual nanofiber network of rigid polylactic acid (PLA) and soft polytetrafluoroethylene (PTFE) in a branched PBAT (CEPBAT) foam.

Repeated occurrences of marine anoxia under high atmospheric O and icehouse conditions.

The Late Paleozoic Ice Age (~340 to 260 Ma) occurred under peak atmospheric O (1.2 to 1.7 PIAL, pre-industrial atmospheric levels) for Earth history and CO concentrations comparable to those of the preindustrial to that anticipated for our near future.

Global cooling drove diversification and warming caused extinction among Carboniferous-Permian fusuline foraminifera.

The fossil record provides the only direct evidence of changes in biodiversity over time. Patterns in more inclusive taxonomic levels (e.g.

Self-assembled enzyme biosensors based on xanthine oxidase (XO) and CS@GO for screening natural products for anti-gout active ingredients.

Gout is a joint disease caused by the deposition of monosodium urate, the incidence of which shows an escalating trend annually while demonstrating a propensity toward younger populations, whereas available pharmacological interventions frequently induce severe adverse effects. In this investigation, a highly sensitive electrochemical enzyme biosensor was constructed based on the pivotal role of xanthine oxidase (XO); the enzymatic activity was enhanced through chitosan - functionalized graphene oxide (CS@GO)-modified electrodes coupled with electrodeposited gold nanoparticles (AuNPs), which, when integrated with their superior electrical conductivity along with exceptional biocompatibility, facilitated the highly efficient screening of anti-gout components within natural products. Following optimization of critical parameters, evaluation of 14 compounds revealed five plant extracts (accounting for 28 % of samples) exhibiting XO inhibition exceeding 83 %, demonstrating superior efficacy compared to allopurinol (83.

Effects of varying longitudinal bending stiffness in running shoes on lower limb biomechanics of elite marathon runners.

Objective: This study examined the effect of three levels of longitudinal bending stiffness (LBS) in carbon-plated shoes on lower limb biomechanics.

Methods: Fifteen elite male marathon runners, each with a personal best under 3 h, participated in the study. They were tested wearing shoes with three LBS levels: low (LLBS, 0.

Lipidomics reveals effect of EHHADH in lung squamous cell.

Lung squamous cell carcinoma (LUSC) and lung adenocarcinoma (LUAD) are two major pathological types of non-small cell lung cancer (NSCLC), characterized by distinct patterns of lipid metabolism. However, the molecular mechanisms underlying lipid metabolism reprogramming specific to LUSC remain poorly understood. This study aims to fill this gap by identifying and characterizing EHHADH (enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase) as a key regulator of medium-chain fatty acid metabolism in LUSC.

Association between brominated flame retardants and obesity: a mediation analysis through markers of oxidative stress and inflammation.

Background: Recent studies have provided compelling evidence that exposure to brominated flame retardants (BFRs) can adversely affect human health. We aim to explore the potential impact of BFRs on adiposity and central obesity.

Methods: Data from the National Health and Nutrition Examination Surveys (NHANES) cycles conducted between 2009 and 2014 was used to study the connections between variables.

Turning Waste into Treasure: Radiation Byproduct-Induced Fe(III)/Fe(II) Conversion for Efficient Ferroptosis to Improve Iodine-131-Based Transarterial Radioembolization for Liver Tumors.

Transarterial radioembolization (TARE) is a primary palliative treatment for advanced liver cancer. Nonetheless, its therapeutic efficacy is frequently hindered by resistance to tumor cell apoptosis induced by inter-radiotherapy. Induction of multiple cell death modalities provides a potential solution to this challenge.

Clinical marine biomedicine: An emerging area in clinical and translational medicine.

Marine biomedicine is an important field in oceanology and bio-ecosystem and has evolved significantly alongside advances in biotechnology and growing understanding of marine life. In this perspective, we propose a refined concept of clinical marine biomedicine, with a clear mission to establish an emerging discipline that bridges marine biomedicine and clinical practice. The exploration of marine-origin sources should be emphasised, with a strong focus on the identification, validation and development of human disease-specific diagnostics and target-oriented pharmaceutics.

An important step to translate single-cell measurement into clinical practice: Stereoscopic cells.

30 April 2025: This article was published in error. The final version of record will be made fully available at a later date.

Chinese Expert Consensus on the Impact of Ambient Air Pollution on Allergic Rhinitis and Recommendations for Mitigation Strategies.

Ambient air pollution poses a significant yet manageable threat to human health. The growing consensus on the impact of ambient air pollutants on allergic rhinitis (AR) emphasizes the importance of prevention, control, and treatment strategies. A multidisciplinary consensus development group was established to further standardize management strategies for AR in the presence of exposure to ambient air pollutants.

The mediating mechanism of Oct4 in the process of cleft palate induced by 2, 3, 7, 8-tetrachlorodibenz-p-dioxin (TCDD) and all-trans retinoic acid.

2, 3, 7, 8-tetrachlorodiphenyl-p-dioxin (TCDD) and all-trans retinoic acid (atRA) have been shown to be inducers of cleft palate (CP). Relevant studies have shown that Oct transcription factor 4 (Oct4) can regulate the expression of the aromatic hydrocarbon receptor (AhR), a target gene of TCDD, as well as influence the retinoic acid signaling pathway. However, the mechanism of Oct4 regulating the interaction between TCDD and atRA in CP pathogenesis remains unclear.