Biofilms: Characteristics and Control Strategies.

The are a family of bacteria that typically cause respiratory, arthritic, and genitourinary disease in humans. spp. of animal origin are also the causative agents of porcine wheezing disease, chronic respiratory disease and arthritis in chickens and other conditions.

Development and validation of a machine learning model for prediction of cephalic dystocia.

Background: Early detection of cephalic dystocia is challenging, and current clinical assessment tools are limited. Machine learning offers unique advantages, enabling the generation of predictive models using various types of clinical data. Our model aims to integrate objective ultrasound data with psychological and sociological characteristics and obstetric treatment data to predict the individual probability of cephalic dystocia in pregnant women.

Never too late to start musical instrument training: Effects on working memory and subcortical preservation in healthy older adults across 4 years.

Studies have shown the beneficial effects of musical instrument on memory and executive function in healthy aging. However, few studies investigated these long-term benefits. In this regard, the current study tracked a cohort of older adults (n = 53) over 4 years after they have initially participated in a musical instrument training program.

Multi-omic analysis reveals the role of coagulation factor family genes and their predictive value for immune checkpoint inhibitors efficacy in gastric cancer.

Coagulation factor family genes play a pivotal role in tumor progression and immune modulation, but their relationship with tumor microenvironment and immunotherapy response in gastric cancer remains unclear. This study utilized single-cell and The Cancer Genome Atlas datasets to investigate the clinical and immune relevance of coagulation factor family genes in gastric cancer. Based on coagulation factor family genes, a risk score was constructed.

Etching-Free Dual Lift-Off for Direct Patterning of Epitaxial Oxide Thin Films.

Although monocrystalline oxide films offer broad functional capabilities, their practical use is hampered by challenges in patterning. Traditional patterning relies on etching, which can be costly and prone to issues like film or substrate damage, underetching, overetching, and lateral etching. In this study, we introduce a dual lift-off method for direct patterning of oxide films, circumventing the etching process and associated issues.

sp. nov., an actinomycete isolated from a phosphate mine.

Two Gram-staining-positive, non-motile and non-spore-forming bacterial strains, designated as 9H2 and YDN13, were isolated from a phosphate mine in Yunnan Province, China. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strains 9H2 and YDN13 belong to the genus . Results show that strains 9H2 and YDN13 occupied a distinct position within the genus and showed the highest 16S rRNA gene sequence similarity of 97.

Metabolic adaptations underlying evolution of Mycoplasma: Nucleotide metabolism and lipid modulation mechanisms driving growth and membrane fluidity in Mycoplasma bovis.

Pathogenic Mycoplasma is a critical pathogen responsible for plant, animal, and human diseases worldwide. Understanding its genetic characteristics and adaptive evolution is essential for elucidating the related transmission mechanisms and pathogenicity. However, analysing the genetic evolution of Mycoplasma solely at the genome level provides an incomplete understanding of the biological characteristics driven by genetic variation.

Component-Resolved Diagnostics for House Dust Mite Allergy: Enhancing Diagnostic Precision and Guiding Subcutaneous Immunotherapy in Children.

Introduction: House dust mite (HDM) sensitization plays a key role in allergic rhinitis (AR) and asthma, yet the clinical value of component-resolved diagnostics (CRD) and immunological markers in children undergoing subcutaneous immunotherapy (SCIT) remains unclear. This study aimed to investigate specific immunoglobulin E (sIgE) and sIgG4 profiles against HDM components to distinguish HDM-induced AR from asymptomatic sensitization and to evaluate the longitudinal changes following SCIT in symptomatic patients, exploring their potential as biomarkers for selecting candidates for SCIT.

Methods: A total of 72 HDM-sensitized participants were included: 46 symptomatic AR patients (with/without allergic asthma) and 26 asymptomatic individuals.

Exploring the effects of the anti-diarrheal formula on intestinal oxidative stress based on network pharmacology, molecular docking, and microbiomics.

The anti-diarrheal formula, a traditional Chinese remedy made from Punica granatum peel, Agrimonia eupatoria, and Gynostemma Blume, contains compounds that reduce oxidative stress. In our previous study, it was shown that the Anti-diarrheal Formula has a therapeutic effect on dysentery in livestock, so follow-up experiments are needed to test the mechanism of action.This study examines the formula's pharmacological mechanisms in treating dysentery through microbiome analysis and network pharmacology to clarify its effects on oxidative stress.

Synovial organoids: From fundamental construction to groundbreaking applications in arthritic disorders.

As an emerging three-dimensional (3D) cell culture model, synovial organoids can highly mimic the structure and function of synovial tissue in vivo, providing a new and powerful tool for the research of synovial-related diseases. This article elaborated in detail on the construction of synovial organoids from the cell sources, culture systems, and construction techniques. Meanwhile, it comprehensively reviewed the application progress of synovial organoids in arthritic diseases including rheumatoid arthritis and osteoarthritis such as disease pathogenesis, drug development, and personalized therapy.

Anomalous metallic state underdoped infinite-layer nickelate superconducting thin films.

To date, the nature of the anomalous metallic state in two-dimensional systems remains unclear. In this study, we present the observation of a quantum superconductor-to-metal transition in underdoped NdSrNiO infinite-layer superconducting thin films, induced by a magnetic field. When a magnetic field perpendicular to the film is applied, we observed a robust intervening anomalous metallic state, characterized by a saturating resistance at low temperatures.

Transcriptome-wide decoding the roles of aberrant splicing in melanoma MAPK-targeted resistance evolution.

Drug resistance critically limits the long-term efficacy of MAPK-targeted therapy in melanoma. While resistance mechanisms at genetic, epigenetic, and transcriptional scales are well-documented, post-transcriptional splicing regulation remains poorly understood. By analyzing patient-matched pre-treatment and resistant melanoma biopsies, we uncover widespread alternative splicing alterations during therapy resistance.

A Prospective Evaluation of the Diagnostic Utility for Low-Coverage Genome Sequencing in Prenatal Samples: A Comparison With Chromosomal Microarray Analysis.

Objective: The present study aimed to evaluate the efficacy of LC-GS in detecting clinically relevant chromosomal abnormalities in comparison with conventional CMA within a prenatal context.

Methods: We conducted a prospective study involving 200 amniotic fluid samples. All specimens were analyzed via LC-GS and traditional tests, including CMA and karyotyping.

Knowledge, attitudes, and practices regarding preconception or early pregnancy single-gene genetic disease screening among reproductive-aged individuals.

Background: This study aimed to assess the knowledge, attitudes, and practices (KAP) regarding preconception or early pregnancy single-gene genetic disease screening among individuals in early pregnancy or preparing for pregnancy.

Methods: A cross-sectional survey was conducted from April 1, 2024, to June 30, 2024, using a validated, self-designed questionnaire which was distributed online to collect data on participants' demographic information and KAP scores.

Results: A total of 562 valid questionnaires were obtained, with 397 (70.

Improved compliance of midwives with stillbirth prevention and control practices after creating and implementing an educational program in a Chinese obstetric clinic.

Background: This study evaluated midwives' compliance with evidence-based stillbirth prevention practices and the impact of a targeted educational intervention. Five audit criteria were established based on the Safer Baby Bundle and expert consensus: (1) smoking cessation support, (2) fetal growth restriction (FGR) screening, (3) fetal movement education for decreased fetal movement, (4) promotion of safe late-pregnancy sleep positions, and (5) risk-based birth timing decisions.

Methods: Using the Joanna Briggs Institute's Practical Application of Clinical Evidence System (PACES) framework, data from 61 midwives in a Chinese obstetric clinic were collected via baseline and follow-up audits.

Integrative Single-Cell Transcriptomics and Network Modeling Reveal Modular Regulators of Sheep Zygotic Genome Activation.

Zygotic genome activation (ZGA) marks the critical transition from reliance on maternal transcripts to the initiation of embryonic transcription early in development. Despite extensive characterization in model species, the regulatory framework of ZGA in sheep remains poorly defined. Here, we applied single-cell RNA sequencing (Smart-seq2) to in vivo- and in vitro-derived sheep embryos at the 8-, 16-, and 32-cell stages.

Comparison of Superovulated Embryo Quality in Simmental Cattle Inseminated with 0 °C-Refrigerated and Liquid Nitrogen-Frozen Semen.

Semen quality plays a crucial role in bovine in vivo embryo production. This study aimed to compare the effects of 0 °C-refrigerated semen and liquid nitrogen-frozen semen on embryo quality in Simmental cattle. Semen collected from five bulls was equally divided into two groups: one diluted with a 0 °C refrigeration solution and stored at 0 °C, and the other diluted with a cryopreservation solution and stored in liquid nitrogen for 24 h.

Estrogen-Regulated Proline-Rich Acidic Protein 1 in Endometrial Epithelial Cells Affects Embryo Implantation by Regulating Mucin 1 in Mice.

This study explores the regulatory role of proline-rich acidic protein 1 () effects on embryo implantation. A high estrogen model and ovariectomized mice were employed to demonstrate that estrogen regulates expression. Uterine tissues were collected from E 1.

LP-003, a novel high-affinity anti-IgE antibody for inadequately controlled seasonal allergic rhinitis: A multicenter, randomized, double-blind, placebo-controlled phase 2 clinical trial.

Background: Anti-IgE therapy can serve as an option for inadequately controlled seasonal allergic rhinitis (SAR) patients. LP-003, a novel anti-IgE antibody, is being tested as an add-on treatment for SAR. This trial aimed to evaluate whether LP-003 is effective and safe for SAR.

Correlation between the use of dried Toad skin-radix clematidis and the development of intestinal dysbiosis and colorectal cancer.

Objective: To examine the impact of different intestinal microbiota conditions on the absorption of couplet medicines (dried toad skin and radix clematidis) into the bloodstream, and to evaluate the therapeutic effects of drug-containing plasma, produced under different intestinal microbiota conditions, on colorectal cancer HT-29 cells.

Methods: In Experiment I, after the pseudo-sterile rat model was established, intragastric administration was performed. Explore the influence of different states of intestinal bacteria on rat organ coefficients, intestine bacteria, plasma metabolites, and so on.

Single-cell transcriptional dissection illuminates an evolution of immunosuppressive microenvironment during pancreatic ductal adenocarcinoma metastasis.

How the host immune system loses its surveillance function during the evolution from normal cell to malignancy is still largely unknown. Here, we investigate the dynamics changes of the pancreatic ductal adenocarcinoma (PDAC) tumor microenvironment by profiling 132,115 single-cell transcriptomes derived from 51 tissues, including healthy pancreatic tissue, non-metastatic PDAC primary tumors, metastatic primary tumors, and patient-matched liver metastases. The cellular proportion, bio-functional, and interaction between each cell type are carefully characterized.

A Novel Variant (c.4354G>A; p. Gly1452Ser) in a Chinese Patient with Developmental Delay, Neurodevelopmental Delay, and Hypotonia.

Introduction: Neurodevelopmental disorders (NDDs) due to the (MIM:617245), the pathogenic variant, are extremely rare. HECW2-related disorder has been established through the identification of de novo variants in gene in patients with NDDs with hypotonia, seizures, and absent language.

Case Presentation: In this study, the clinical and genetic features of a Chinese girl with neurodevelopmental delay, developmental language disorder, and hypotonia are described.

Spin Manipulation of Single Nitroxide Radical on Au(111) by Selective Coordination.

Organic radicals are promising candidates for constructing molecule-based magnetic materials. Despite the achievements of various radical ligand-containing complex materials, tuning the spin state of organic radicals at the single-molecule level on surfaces remains a challenge. In this study, the spin state of the DPBIN molecule, a derivative of 1,4-di(pyridine-3-yl)benzene bearing two imino-nitroxide radicals in para positions, is tuned by selective coordination with Au, Ni, and Fe centers on the Au(111) surface.

Predicting the risk of postoperative gastrointestinal bleeding in patients with Type A aortic dissection based on an interpretable machine learning model.

Background: Gastrointestinal bleeding (GIB) is a common complication following Type A aortic dissection (TAAD) surgery, significantly impacting prognosis and increasing mortality risk. This study developed and validated a predictive model based on machine learning (ML) algorithms to enable early and precise assessment of postoperative GIB risk in TAAD patients.

Methods: Medical records of patients who underwent TAAD surgery at Shanxi Bethune Hospital from January 2019 to September 2024 were retrospectively collected.