Characterization of the Specific Binding Between Aptamers and Cytochrome c With Pressure-Assisted Capillary Electrophoresis Frontal Analysis.

Cytochrome c (cyt c) is a heme protein located in the mitochondrial intermembrane space. Because the release of cyt c is a highly specific event in apoptotic signaling, it can serve as an apoptosis-related marker. To date, three frequently used aptamers for cyt c (Apt40, Apt61, and Apt76) have been selected and applied in the field of sensing.

Danhong Injection targets CaMKII through Dihydrotanshinone I to alleviate cardiomyocyte death and inflammation in viral myocarditis.

Viral myocarditis, an inflammatory heart disorder caused by viral infections, often leads to poor outcomes due to the lack of effective prevention and treatment strategies. Despite the characteristic feature of cardiomyocyte death in this condition, the underlying mechanisms and potential therapeutic approaches remain poorly understood. Traditional Chinese medicine (TCM), despite its extensive history in treating various diseases, has not yet been approved for the treatment of viral myocarditis.

Varicella-Zoster Virus Infection and Varicella-Zoster Virus Vaccine-Related Ocular Complications.

The varicella-zoster virus is a human herpesvirus that causes varicella as the primary infection and HZ as the reactivation of a latent infection. Ten to twenty percent of cases of herpes zoster ophthalmicus (HZO) involve the ophthalmic branch of the fifth cranial nerve. Any area of the eye may be affected by the condition.

Clinical Characteristics and Influencing Factors of Postoperative Pain in Patients Undergoing Gastric Endoscopic Submucosal Dissection.

Background: To comprehensively analyze the clinical characteristics of patients who underwent gastric endoscopic submucosal dissection (ESD) and explore the incidence and influencing factors of postoperative pain.

Methods: The clinical data of patients who underwent gastric ESD at our center from 2009 to 2024 were retrospectively analyzed. Pain severity was assessed using a visual analogue scale, with a score ≥ 4 defined as postoperative pain.

Hypericin attenuates myocardial ischemia-reperfusion injury by enhancing mitochondrial energy metabolism via AMPK/PGC-1α signaling pathway.

Background: Although blood flow is restored following the treatment of acute myocardial infarction (AMI), myocardial Ischaemia /reperfusion can still cause cardiac damage, potentially leading to cardiac decompensation and, ultimately, heart failure. This study aimed to investigate the effects of Hypericin (Hyp) on the hearts of rats with myocardial ischaemia-reperfusion injury (MIRI) and its underlying mechanisms.

Methods: Male Sprague-Dawley rats and H9C2 cells underwent MIRI and hypoxia/reoxygenation (H/R) modelling after Hyp administration to assess the compound's effects on cardiac and cardiomyocyte characteristics, as well as mitochondrial energy metabolism.

Emergence, Molecular Characteristics and Resistance Mechanisms of Colistin-Resistant in Xuzhou, China.

Background: Carbapenem-resistant Enterobacterales (CRE) infections pose a significant global public health threat, with colistin as the last line of defense. Increasing colistin resistance presents a formidable clinical challenge. This study aimed to elucidate the molecular characteristics and resistance mechanisms of clinical colistin-resistant (ColR) CRE strains in Xuzhou, China.

BMP Signaling Alleviates Allergic Airway Inflammation by Controlling Group 2 Innate Lymphoid Cells Homeostasis.

Recent studies have highlighted the important role of bone morphogenetic proteins (BMPs) in immunoregulation. Our earlier work identified the expression of BMP receptors on lymphoid progenitors and group 2 innate lymphoid cells (ILC2s) in the bone marrow. However, the precise function of BMP signaling in the development and activity of ILC2s remains unclear.

EXGEP: a framework for predicting genotype-by-environment interactions using ensembles of explainable machine-learning models.

Phenotypic variation results from the combination of genotype, the environment, and their interaction. The ability to quantify the relative contributions of genetic and environmental factors to complex traits can help in breeding crops with superior adaptability for growth in varied environments. Here, we developed and extensively evaluated the performance of an explainable machine-learning framework named explainable genotype-by-environment interactions prediction (EXGEP) to accurately predict the grain yield in crops.

Non-Random Distribution of EMS-Induced Mutations Reveals Preference for Open Chromatin and Expressed Genes in Rice.

Mutagenesis is an effective strategy to generate genetic resources for functional genomics and crop improvement. It is widely accepted that chemical-induced mutations are randomly distributed across the genome. However, the randomness of such mutations remains a matter of scientific debate due to a lack of systematic investigation.

Real-World Pharmacological Treatment Pattern of Neuropathic Pain in China: A Retrospective, Database, Multicenter Study (ReTARdant) Protocol.

Introduction: Neuropathic pain (NP), including diabetic peripheral neuropathic pain (DPNP) and chemotherapy-induced peripheral neuropathy (CIPN) as common subtypes, imposes a significant clinical burden on patients, severely affecting their quality of life. The real-world evidence on demographic characteristics, treatment patterns, and adherence data of DPNP and CIPN is still limited.

Methods: A multicenter, retrospective, observational study using electronic medical record (EMR) systems involving approximately 600 patients with DPNP and 400 with CIPN from nine hospitals was planned.

hsa_circ_0002872 functions in lung cancer prognostic and tumorigenesis through regulating ZBTB46 via sponging hsa-miR-29b-1-5p.

Background: Lung cancer is a leading cause of cancer deaths worldwide, underscoring the need for new biomarkers. Circular RNAs (circRNAs) exhibit potential as biomarkers for lung cancer. ZBTB46 is linked to lung cancer prognosis.

SIRT4-Mediated Deacetylation of PRDX3 Attenuates Liver Ischemia Reperfusion Injury by Suppressing Ferroptosis.

Liver ischemia-reperfusion injury (LIRI) is an important cause of the clinical prognosis of liver transplantation. Despite Sirtuin 4 (SIRT4) is involved in various post-translational modifications, its role in LIRI is unclear. This research aimed to investigate the influence of SIRT4 on the pathogenesis of LIRI.

Robust calibration method for polarization cameras integrated with chromatic retarders.

Polarization cameras provide a compact and efficient solution for real-time polarization imaging but suffer from chromatic calibration errors when used in combination with wavelength-dependent optical components such as linear retarders. In this work, we introduce an analytical calibration method specifically designed for polarization cameras integrating chromatic retarders, which can be used both in monochromatic and color sensors. This method algebraically accounts for spectral averaging and depolarization effects arising from wavelength-dependent retardance, enabling robust and accurate polarization calibration across different spectral channels.

SLC39A13 Regulates Heart Function via Mitochondrial Iron Homeostasis Maintenance.

Background: Iron is a necessary trace element for multiple reactions but is toxic in excess. Its intracellular balance is delicately maintained. We previously found that the loss of SLC39A13 (solute carrier family 39 member 13)/ZIP13 (zinc-iron permease 13), a newly identified endoplasmic reticulum/Golgi-resident iron transporter, impacted iron homeostasis in multiple tissues.

A Novel Polysaccharide from Blackened Jujube: Structural Characterization and Immunoactivity.

Previously, research adopted an ultrasound-assisted extraction method to isolate crude polysaccharide from blackened jujube, followed by preliminary structural identification of the purified polysaccharide (BJP). This manuscript analyzed the accurate structure and immunomodulatory activity of BJP. Further structural identification indicated that BJP was mainly composed of →3)-α-L-Ara-(1→, →3,5)-α-L-Ara-(1→, →3)-β-D-GalA-(1→, →2,4)-β-D-Gal-(1→, →4)-β-D-GalA-(1→, →3)-α-L-Rha-(1→ and →3,4)-α-L-Rha-(1→.

Plasma Soluble ST2 as a Prognostic Biomarker for Cardiovascular Events and Mortality in COVID-19 Patients.

Background: Coronavirus disease 2019 (COVID-19) is frequently complicated by cardiovascular involvement. Soluble growth stimulation-expressed gene 2 (sST2) is a promising cardiovascular biomarker, but its prognostic value in COVID-19 remains unclear.

Methods: This retrospective cohort study included 314 hospitalized COVID-19 patients classified into mild/moderate ( = 168) and severe/critical ( = 146).

Selective ratiometric fluorescent detection of chlortetracycline in environmental water based on hydrolysis-induced emission.

For specific determination of chlortetracycline (CTC) among tetracycline antibiotics (TCs), the current study focused on the hydrolysis effect of TCs. In water, CTC was hydrolyzed into isochlortetracycline (iso-CTC) along with blue fluorescence, but other TCs remained stable without blue fluorescence. CTC was thus identified within TCs based on hydrolysis-induced emission.

The association of essential dietary trace elements and their mixture with cognition: a prospective study.

Background: The association of dietary essential trace elements (ETEs) and their mixture with cognition remains unclear.

Methods: Prospective cohort data on the association between dietary ETEs [e.g.

The study on the molecular characteristics and variation patterns of the recombinant Muscovy duck parvovirus strain GD-23.

As the virus continues to spread, the frequency of contact between different parvovirus strains has increased. In recent years, recombinant Muscovy duck parvovirus (rMDPV) has been widely prevalent in China. In 2023, a strain of rMDPV, designated GD-23, was isolated from Guangdong.

Bidirectional Mediation of Cognition by DNA Methylation and Lean Body Mass in Chinese Monozygotic Twins.

This study explores whether DNA methylation (DNAm) mediates the association between lean body mass (LBM) and cognition, as well as whether LBM mediates the association between DNAm and cognition. Based on the data of 59 monozygotic twin pairs, mediation analyses were performed using causal inference test method and mediation analyses. Average causal mediation effect (ACME), average direct effect (ADE), and total effect (TE) were calculated.

Proanthocyanidin-Driven Multifunctional Injectable Hydrogel as an Effective Skin Dressing for Atopic Dermatitis.

Patients with atopic dermatitis (AD) often experience symptoms of skin dryness, coinfection, oxidative damage, immune dysfunction, and inflammatory response. Clinical recommendations typically include antibiotics, glucocorticoids, and immunosuppressants, but frequent use of these medications can lead to drug resistance and adverse side effects. Herein, a pH/ROS-responsive injectable dual network hydrogel (HCFP) was developed by cross-linking oxidized hyaluronic acid, quaternized chitosan, and proanthocyanidins (PA) for the treatment of atopic dermatitis (AD).

Methyl Viologen@β-Zeolite with Absorption/Fluorescence Dual-Mode and Photo/Chemical Synergistic Stimuli-Responsive Chromism.

In this work, methyl viologen (MV) was adsorbed into the nanopores of Si/Al H-β-zeolite via cation exchange. The resulting MV@β-zeolite possessed absorption/fluorescence dual-mode and photo/chemical synergistic stimuli-responsive chromism. Owing to the acidic surrounding provided by β-zeolite, the chromism of MV required the synergistic stimuli of UV irradiation and a chemical reductant (such as NaSO).

Phenylpropanoids metabolism: recent insight into stress tolerance and plant development cues.

The phenylpropanoid pathway remains a key target for most climate-resilient crop development, owing to it being a precursor to over 8000 metabolites, including flavonoids and lignin compounds, including their derivatives. These metabolites are involved in biotic and abiotic stress tolerance, inviting several studies into their roles in plant defense, drought, temperature, UV, and nutrient stress tolerance. Literature is currently inundated with cutting-edge reports on the phenylpropanoid pathways and their functions.

Prosthesis survival situation and complications following total hip arthroplasty in hemophilic patients: a systematic review.

Background: Hemophilic arthropathy, resulting from recurrent joint bleeding in patients with hemophilia, often necessitates total hip arthroplasty (THA) to alleviate pain and improve joint function. However, the unique challenges posed by hemophilia, including coagulopathy and compromised bone health, result in increased risks of complications such as bleeding, infection, and prosthesis loosening.

Methods: A systematic search of PubMed, Cochrane Library, EMBASE, and Web of Science was conducted up to September 2024.