Pancreatic Elastase Affects Liver Injury by Activating Pro-Inflammatory Cytokines in Kupffer Cells via the JAK2/STAT3 Signaling Pathway.

Introduction: This study aimed to investigate the role of JAK2 (Janus kinase 2)/STAT3 (signal transducer and activator of transcription 3) signaling in liver injury during severe acute pancreatitis (SAP), focusing on pancreatic elastase- and lipopolysaccharide (LPS)-induced Kupffer cell (KC) activation.

Methods: A rat SAP model was established via retrograde taurocholic acid infusion into the biliopancreatic duct. Inflammatory cytokine levels and JAK2/STAT3 pathway activity were quantified in liver tissues.

Predicting the risk of postoperative gastrointestinal bleeding in patients with Type A aortic dissection based on an interpretable machine learning model.

Background: Gastrointestinal bleeding (GIB) is a common complication following Type A aortic dissection (TAAD) surgery, significantly impacting prognosis and increasing mortality risk. This study developed and validated a predictive model based on machine learning (ML) algorithms to enable early and precise assessment of postoperative GIB risk in TAAD patients.

Methods: Medical records of patients who underwent TAAD surgery at Shanxi Bethune Hospital from January 2019 to September 2024 were retrospectively collected.

MAGL targeted PROTAC degrader simultaneously enhances P53 for synergistic treatment of glioblastoma stem cell.

Glioblastoma (GBM) stands as the most fatal brain tumor due to limited therapeutic options and high rates of drug resistance. Current surgical and pharmacological interventions usually fail to eradicate the aggressive GBM stem cells (GSCs), which leads to the deadly GBM occurrence. Although proteolysis-targeting chimeras (PROTACs) are prosperous in drug development for tumors, their application in GBM, particularly for GSC-sensitive drug candidates remains in its nascent stages.

CD97 maintains tumorigenicity of glioblastoma stem cells via mTORC2 signaling and is targeted by CAR Th9 cells.

Glioblastoma (GBM) stem cells (GSCs) contribute to poor prognosis in patients with GBM. Identifying molecular markers is crucial for developing targeted therapies. Here, we identify cluster of differentiation 97 (CD97) as an optimal GSC surface antigen for potential targeting by chimeric antigen receptor (CAR) T cell therapy through in vitro antibody screening.

Maternal Vitamin A deficiency during pregnancy and lactation induced damaged intestinal structure and intestinal flora homeostasis in offspring mice.

The small intestine serves as the first channel of dietary Vitamin A (VA) and the unique organ of VA absorption and metabolism. However, there have not been extensive investigations on the exact mechanisms within VA-related changes in intestinal metabolic disorders. This research is designed to analyze whether and how VA affects intestinal metabolic phenotypes.

2-Methoxy-1,4-naphthoquinone regulated molecular alternation of revealed by high-dimensional biological data.

Fungi and the toxins it produces are hazardous to agricultural plants, animals, and human health. However, there is a lack of more effective and environment-friendly natural anti- agents. In the search for natural anti-fungal agents, we found that naturally originated 2-methoxy-1,4-naphthoquinone (MNQ) with a minimal inhibitory dose of 8.

Biochar enhanced polycyclic aromatic hydrocarbons degradation in soil planted with ryegrass: Bacterial community and degradation gene expression mechanisms.

Biochar and ryegrass have been used in the remediation of polycyclic aromatic hydrocarbons (PAHs)-contaminated soils; however, the effects of different biochar application levels on the dissipation of PAHs, bacterial communities, and PAH-ring hydroxylating dioxygenase (PAH-RHDα) genes in rhizosphere soil remain unclear. In this study, enzyme activity tests, real-time quantitative polymerase chain reaction (PCR), and high-throughput sequencing were performed to investigate the effects of different proportions of rape straw biochar (1%, 2%, and 4% (w/w)) on the degradation of PAHs, as well as the associated changes in the soil bacterial community and PAH-RHDα gene expression. The results revealed that biochar enhanced the rhizoremediation of PAH-contaminated soil and that 2% biochar-treated rhizosphere soil was the most effective in removing PAHs.

Efficacy and economics evaluation of seed rhizome treatment combined with preplant soil fumigation on ginger soilborne disease, plant growth, and yield promotion.

Background: Ginger (Zingiber officinale Roscoe) is widely planted around the world. Owing to continuous planting, ginger is seriously affected by soilborne fungi, bacteria, and nematodes. Although preplant soil fumigation is an effective prevention strategy of soilborne diseases, individual fumigant and technology could not provide effective control of ginger soilborne disease.

Isoacteoside attenuates acute kidney injury induced by severe acute pancreatitis.

Severe acute pancreatitis (SAP) is a common acute abdominal disease accompanied by systemic inflammatory response syndrome, which may be complicated by acute kidney injury (AKI). Isoacteoside (ISO) is the active ingredient of Franch. ex Maxim and has been reported to have anti‑inflammatory activities.

Investigation of Physicochemical Indices and Microbial Communities in Termite Fungus-Combs.

species are wild edible mushrooms that possess high nutritional value and a wide range of medicinal properties. However, the cultivation of these mushrooms is very difficult because of their symbiotic association with termites. In this study, we aimed to examine the differences in physicochemical indices and microbial communities between combs with basidiomes (CF) and combs without basidiomes (CNF).

2-Methoxy-1,4-naphthoquinone Induces Metabolic Shifts in Revealed by High-Dimensional Biological Data.

is the primary pathogen causing the green mold of citrus. The need for the development of higher effective and lower toxic natural antifungal agents is urgent, owing to the lack of antifungal agents that can successfully combat . Herein, the effects and mechanisms of 2-methoxy-1,4-naphthoquinone (MNQ) as a potential inhibitor of were studied.

Termitomyces heimii Associated with Fungus-Growing Termite Produces Volatile Organic Compounds (VOCs) and Lignocellulose-Degrading Enzymes.

Termitomyces fungi associated with fungus-growing termites are the edible mushrooms and can produce useful chemicals, enzymes, and volatile organic compounds (VOCs) that have both fuel and biological potentials. To this purpose, we examined the Termitomyces mycelial growth performance on various substrates, clarified lignocellulose-degrading enzyme activity, and also identified the VOCs produced by Termitomyces. Our results indicated that the optimal nutrition and condition requirements for mycelial growth are D-sorbitol, D-(+)-glucose, and D-(-)-fructose as carbon sources; peptone as well as yeast extract and ammonium tartrate as nitrogen sources; and Mn, Na, and Mg as metal ions with pH range from 7.

Dimethyl disulfide (DMDS) as an effective soil fumigant against nematodes in China.

Root-knot nematode is an important soil pest in horticulture crops and constrains the protected cultivation development after methyl bromide (MB) was phased out in China. Dimethyl disulfide (DMDS) exhibits excellent efficacy against nematodes. Laboratory experiments and field trials were set up to clarify DMDS dose, efficacy, and yield.

Label-Free Proteomic Analysis of Molecular Effects of 2-Methoxy-1,4-naphthoquinone on .

is the principal pathogen causing blue mold of citrus. Searching for novel antifungal agents is an important aspect of the post-harvest citrus industry because of the lack of higher effective and low toxic antifungal agents. Herein, the effects of 2-methoxy-1,4-naphthoquinone (MNQ) on and its mechanism were carried out by a series of methods.

Effect of JAK2/STAT3 signaling pathway on liver injury associated with severe acute pancreatitis in rats.

Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling constitutes one of the major pathways for cytokine signal transduction. However, the role of the JAK2/STAT3 pathway in liver injury during severe acute pancreatitis (SAP) remains unclear. The aim of this study was to investigate the role of the JAK2/STAT3 signaling pathway in liver injury after SAP.

Enhanced polycyclic aromatic hydrocarbons degradation in rhizosphere soil planted with tall fescue: Bacterial community and functional gene expression mechanisms.

To investigate the bacterial mechanisms of polycyclic aromatic hydrocarbons (PAHs) degradation in an aged-contaminated agricultural soil planted with tall fescue (Festuca arundinacea), a rhizo-box experiment was carried out for 60 d. Shifts in bacterial community structure in the soils during the experiment were performed using denaturing gradient gel electrophoresis. The abundance and activity of total bacteria and PAH-degraders were measured by quantification of 16S rDNA, PAH-ring hydroxylating dioxygenase (PAH-RHDα) genes and their transcripts, respectively.

A highly selective fluorescent probe for the detection of hypochlorous acid in tap water and living cells.

A turn-on fluorescent probe (DAME) for sensing hypochlorous acid (HClO) with excellent selectivity was presented. The fluorescent probe was composed of coumarin derivative as the fluorophore and dimethylcarbamothioic chloride group with a sulfide moiety as modulator. Additionally, the sulfide moiety would be oxidized by HClO, and then free dye of coumarin derivate was released and exhibited significant fluorescence.

Effect of soil fumigants on degradation of abamectin and their combination synergistic effect to root-knot nematode.

Background: Root-knot nematode (Meloidogyne spp., RKN) causes a disease that significantly reduces the yield of greenhouse cucumber crops year after year. Chemical control based on a single pesticide is now unreliable mainly due to pest resistance.

Evaluation of allyl isothiocyanate as a soil fumigant against soil-borne diseases in commercial tomato (Lycopersicon esculentum Mill.) production in China.

Background: Root-knot nematodes (Meloidogyne spp.), soil-borne diseases and weeds seriously reduce the commercial yield of tomatoes grown under protected cultivation in China. Allyl isothiocyanate (AITC), a natural product obtained from damaged Brassica tissues, was evaluated as a potential replacement for the fumigant methyl bromide (MB) for use in the greenhouse production of tomatoes in China.

Effects of ω-3 Fatty Acids on Toll-like Receptor 4 and Nuclear Factor κB p56 in the Pancreas of Rats With Severe Acute Pancreatitis.

Objectives: The aims of this study were to determine the effects of ω-3 fatty acids (ω-3FAs) on the Toll-like receptor 4 (TLR4)/nuclear factor κB p56 (NF-κBp56) signaling pathway in the pancreas of rats with severe acute pancreatitis (SAP).

Methods: Sixty-four Sprague-Dawley rats were randomly divided into 4 groups: the control, SAP-saline, SAP-soybean oil, and SAP-ω-FA groups. Severe acute pancreatitis was induced by the retrograde infusion of sodium taurocholate into the pancreatic duct.