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A turn-on fluorescent probe (DAME) for sensing hypochlorous acid (HClO) with excellent selectivity was presented. The fluorescent probe was composed of coumarin derivative as the fluorophore and dimethylcarbamothioic chloride group with a sulfide moiety as modulator. Additionally, the sulfide moiety would be oxidized by HClO, and then free dye of coumarin derivate was released and exhibited significant fluorescence. In addition, the probe could respond to HClO in solutions within 60 s and the limit of detection was down to 34.75 nM. Moreover, the probe was used for the detection of HClO in tap water through the home-made test paper. And confocal images confirmed that probe DAME could be used for recognizing HClO in living cells.
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http://dx.doi.org/10.1016/j.saa.2018.06.012 | DOI Listing |
Photochem Photobiol Sci
September 2025
Faculity of Engineering, Yokohama National University, 79-5, Tokiwadai, Hodogaya, Yokohama, Kanagawa, 240-8501, Japan.
In recent years, fluorescence-switchable molecules have garnered significant attention as fluorescent dyes for super-resolution fluorescence microscopy, which is increasingly demanded in the field of biochemical imaging. Among such molecules, diarylethene-S,S,S',S'-tetraoxide derivatives have proven particularly promising due to their ability to achieve high contrast fluorescence switching. Diarylethenes incorporating perfluorocyclopentene as the ethene bridge have become the standard scaffold due to their excellent fatigue resistance and thermal stability.
View Article and Find Full Text PDFChem Pharm Bull (Tokyo)
September 2025
Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Antigen-binding proteins, such as nanobodies, modified with functional small molecules hold great potential for applications including imaging probes, drug conjugates, and localized catalysts. However, traditional chemical labeling methods that randomly target lysine or cysteine residues often produce heterogeneous conjugates with limited reproducibility. Conventional site-specific conjugation approaches, which typically modify only the N- or C-terminus, may also be insufficient to achieve the desired functionalities.
View Article and Find Full Text PDFAnal Chim Acta
November 2025
Jiangxi Provincial Key Laboratory of Organic Functional Molecules, Institute of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, 330013, PR China; Department of Ecology and Environment, Yuzhang Normal University, Nanchang, 330103, PR China. Electronic address: pushouzhi
Background: The hydrogen sulfide (HS) in spoilage of raw meat poses significant food safety risks to human health. Meanwhile, as a signaling molecule, HS is crucial for maintaining human physiological homeostasis. Thus, the establishment of an efficient method for HS detection is essential for safeguarding human health.
View Article and Find Full Text PDFAnal Chim Acta
November 2025
State Key Laboratory of Materials-oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, Nanjing, 211816, PR China; Zhangjiagang Institute of Nanjing Tech University, Suzhou, 215600, PR China. Electronic address:
Background: Zinc (Zn) and cadmium (Cd) ions are ubiquitous in industrial and daily life. Despite their critical impact on food safety and human health, current probes face significant limitations in simultaneously detecting both ions in complex food matrices.
Results: Herein, we successfully developed a pyrene-based FRET ratiometric fluorescent probe QP for the highly selective detection of Zn and Cd.
Anal Chim Acta
November 2025
The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou, 215123, PR China; Center of Self-Propelled Nanotechnologies, Suzhou Industrial Park Institute of Services Outsourcing, Suzhou, 215123, PR China
Background: Of the mycotoxins, aflatoxin is the most significant. The detection of aflatoxin B1 (AFB1) is crucial for ensuring food safety, as this highly carcinogenic toxin readily contaminates crops such as grains and nuts, and timely detection can effectively prevent associated health risks. The selection of luminophores is of paramount importance in the detection of ECL (electrochemiluminescence).
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