Mitochondria-targeted NIR fluorescent probe for detection of hydrogen sulfide and its application in monitoring food freshness and bioimaging.

Anal Chim Acta

Jiangxi Provincial Key Laboratory of Organic Functional Molecules, Institute of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, 330013, PR China; Department of Ecology and Environment, Yuzhang Normal University, Nanchang, 330103, PR China. Electronic address: pushouzhi

Published: November 2025


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Article Abstract

Background: The hydrogen sulfide (HS) in spoilage of raw meat poses significant food safety risks to human health. Meanwhile, as a signaling molecule, HS is crucial for maintaining human physiological homeostasis. Thus, the establishment of an efficient method for HS detection is essential for safeguarding human health.

Results: In this work, a NIR fluorescent probe TPAW (emission at 690 nm) based on ICT effect for detection of HS was constructed. TPAW exhibited fast response (<10 s), high selectivity, high sensitivity, good biocompatibility, and mitochondrial targeting ability. The testing mechanism was thoroughly validated by H NMR and ESI-MS. In addition, application studies demonstrated that TPAW can effectively monitor HS produced during food spoilage under 365 nm UV light via portable test strips based on smartphone analyze. Furthermore, TPAW was successfully employed for imaging HS in A549 cells and zebrafish, demonstrating its potential utility in biomedical research.

Significance: This study provides a promising and effective tool for applications and research in biomedical application and food safety.

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http://dx.doi.org/10.1016/j.aca.2025.344467DOI Listing

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