A barcode-specific immobilization interface for microfluidics-assisted uniform spatially barcoded microarray analysis.

Microfluidics-assisted spatially barcoded microarray technology offers a high-throughput, low-cost approach towards spatial transcriptomic profiling. A uniform barcoded microarray is crucial for spatially unbiased mRNA analysis. However, non-specific adsorption of barcoding reagents in microchannels occurs during liquid transport, causing non-uniform barcoding in the chip's functional regions.

Viscosity-sensitive fluorescent probes based on the hemicyanine for the organelle-specific visualization during autophagy and ferroptosis.

The dynamic monitoring of cell death processes remains a significant challenge due to the scarcity of highly sensitive molecular tools. In this study, two hemicyanine-based probes (5a-5b) with D-π-A structures were developed for organelle-specific viscosity monitoring. Both probes exhibited correlation with the Förster-Hoffmann viscosity-dependent relationship (R > 0.

Fluorescence/Colorimetric Dual-Mode Probe Based on Nitrogen, Sulfur, and Boron Codoped MXene Quantum Dots for Detection of Glutathione and Imaging in Mitochondria.

Quantifying and monitoring glutathione (GSH) in living cells is of great significance for understanding GSH-related oxidative stress disorders and neoplastic conditions. In this work, we developed nitrogen/sulfur/boron codoped MXene quantum dots (NSB-MQDs) through a facile single-pot hydrothermal synthesis strategy for fluorescence/colorimetric detection and imaging of GSH. The fluorescence "Turn-On" sensing signal was generated by the electron or energy transfer process between GSH and NSB-MQDs, which exhibited a fluorescence quenching phenomenon with the increase of GSH.

Natural quercetin-derived carbon nanodots for dual antibacterial and anti-inflammatory therapy against bacterial infections.

Bacterial infections and the associated inflammatory responses present significant challenges to public health, underscoring the need for innovative therapeutic strategies. In this study, novel carbon dots (QA-CDs) derived from quercetin (QU) and 4-aminophenol (4-AP) were synthesized using a one-step hydrothermal method. This approach merges the antimicrobial properties of phenolic compounds with the multifunctional advantages of carbon-based nanomaterials.

Homogeneous Electrochemical Enzyme-Linked Immunosorbent Assay Strategy Based On pH-Mediated Redox Potential Regulation.

Enzyme-linked immunosorbent assay (ELISA) is widely recognized as the gold standard for protein detection. However, its reliance on expensive and bulky optical instruments limits its use in point-of-care and resource-limited settings. Electrochemical technique emerges as a promising alternative due to its low cost, portability, and simple instrumentation.

Multimodal dynamic hierarchical clustering model for post-stroke cognitive impairment prediction.

Post-stroke cognitive impairment (PSCI) is a common and debilitating consequence of stroke that often arises from complex interactions between diverse brain alterations. The accurate early prediction of PSCI is critical for guiding personalized interventions. However, existing methods often struggle to capture complex structural disruptions and integrate multimodal information effectively.

Profiling of lipid mediators and oxylipins in SARS-CoV-2 infection associated thrombosis.

Thrombosis is a life-threatening complication in Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection. This study aims to conduct a statistical analysis of the incidence of blood clots and lipid concentrations, and to examine the networks of oxylipins in hospitalised patients with SARS-CoV-2. Serum samples of 1731 hospitalised patients with SARS-COV-2 were used to measure six lipid parameters: total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A (apoA), and apolipoprotein B (apoB).

Pulse counting of nanobubbles inside DNA-functionalized nanopores and the application for highly sensitive electroanalysis.

In conventional electrochemical biosensors, nanobubbles generated by electrochemical reactions typically adhere to the electrode interface, which affect the accuracy of measurements. In this study, we take good use of nanobubbles as the signal source and effectively address this limitation. An ultrasensitive approach for the detection of miRNA is further developed coupling catalytic hairpin assembly and DNA-functionalized nanopores to generate nanobubbles.

High scattering sensitivity entropy imaging for breast tumor characterization and classification.

Background: Diagnosing and characterizing breast lesions and tumors remains a common challenge in clinical practice. Ultrasound imaging stands out for its safety, real-time capability, and affordability. However, the image quality of conventional ultrasound examination is limited, and the diagnosis of ultrasonographic images depends heavily on the experience of the sonographer.

Design of a low-power gradient amplifier for benchtop nuclear magnetic resonance spectrometer.

Benchtop Nuclear Magnetic Resonance (BNMR) technology has gained increasing attention in chemical and pharmaceutical applications due to its compact configuration and operational flexibility. However, the implementation of conventional commercial gradient amplifiers in BNMR systems remains challenging because of their excessive power consumption and bulky dimensions. To address these limitations, this study presents a novel gradient amplifier design optimized for BNMR applications, characterized by a satisfactory linearity in 0-2.

Precision Engineering of Bioceramics.

Precision medicine aims to provide specific and personalized treatment strategies for patients, the realization of which depends highly on the integration of materials science and advanced biofabrication technologies. Bioceramics, as one of the typical biomaterials, have been widely used in biomedical fields due to their excellent bioactivity and biocompatibility. However, traditional "one-size-fits-all" fabrication of bioceramics falls short of meeting the demands of precision medicine.

CMVFT: A Multiscale Attention-Guided Framework for Enhanced Keratoconus Suspect Classification in Multiview Corneal Topography.

Objective: To develop a multiview fusion framework that effectively identifies suspect keratoconus cases and facilitates the possibility of early clinical intervention.

Design: Retrospective cross-sectional study.

Subjects: A total of 573 corneal topography maps representing eyes classified as normal, suspect, or keratoconus.

ACSL3/GABARAPL2 Ameliorates Vascular Endothelial Cell Aging and Injury Through Protective Autophagy to Alleviate Ferroptosis.

Vascular endothelial cell (VEC) dysfunction is a key contributor to vascular diseases. Oxidative stress-induced ferroptosis accelerates VEC aging and injury, while protective autophagy mitigates this damage by clearing damaged organelles and reducing oxidative stress. GABARAPL2, a critical autophagy-related protein, and ACSL3, a regulator of lipid metabolism and ferroptosis, are emerging as interconnected mediators of cellular protection.

Advances in engineered organoid models of skin for biomedical research.

In recent years, significant progress has been made in the development of organoids, which offer promising opportunities for developmental and translational research. With advances in cell biology and bioengineering techniques, skin models are evolving from conventional multilayered structures to appendage-bearing spheroids or 3D biomimetic models. This comprehensive review aims to provide an in-depth understanding of organoid models of the skin, covering topics such as skin development, construction strategies and key elements, types of organoid models, biomedical applications, and challenges.

Spacing Controlled Quantum Resonance in Colloidal CdSe Quantum Dot Superlattices.

Understanding the correlation between interdot spacing and their interactions is crucial for controlling the properties of quantum dot superlattices. In this study, the interdot spacing of the colloidal quantum dot superlattice is tuned from 1.4 to 0.

Promoting implant osseointegration via the osteoblast-selective β-amino acid polymer strategy.

Osseointegration for implants, especially bioinert implants, poses significant clinical challenges. Overcoming fibrotic encapsulation and promoting osseointegration at the implant interface are critical for successful bone repair, which highly expected biomaterials with osteoblast over fibroblast selectivity. However, few materials possess the function.

Evaluating the effectiveness of flight simulator training on developing perceptual-motor skills among flight cadets: a pilot study.

This study aimed to evaluate the impact of flight simulator training on the enhancement of perceptual-motor skills among flight cadets. Perceptual-motor skills act as a crucial link through which pilots translate their environmental perceptions into precise maneuvers, a capability that is particularly vital in dynamic and unpredictable flight environments. A total of forty cadets participated in the experiment and were randomly assigned to either the Traditional Training Group (TTG) or the Efficient Training Group (ETG).

DNA Triangular Pyramid Frustum Supported miR-21 Assay with Duplex-Specific Nuclease-Assisted Target Recycling.

MicroRNAs (miRNAs) have the potential to be applied as effective biomarkers for early diagnosis of cancers. Electrochemical techniques exhibit advantages such as high sensitivity and ease of miniaturization. However, electrode interface perturbations may hinder electrochemical responses.

Diabetes-Associated Changes in the Toe Photoplethysmogram During Local Heating Test.

We investigated changes in the pulse waveform recorded by photoplethysmography (PPG) during a local heating test in patients with diabetes. The following groups of subjects were studied: healthy individuals (n = 15), patients with Type 2 diabetes accompanied by diabetic retinopathy (n = 14), patients with Type 2 diabetes and diabetic foot syndrome (n = 16). Measurements were taken from the big toes of both limbs while the skin was heated from 32°C to 42°C.

Facile fabrication of antioxidative and antibacterial hydrogel films to accelerate infected diabetic wound healing.

Diabetic wounds are accompanied with hemorrhage, excessive reactive oxygen species accumulation, microbial infection, and poor exudate management. Current dressings are insufficient to modulate the complex pathophysiological microenvironment of infected diabetic wounds. Here, we develop a facile-fabricated hydrogel film with potent antibacterial and antioxidative properties to significantly accelerate infected diabetic wounds healing.

Benzonase-Based Closed-Tube DNA Degradation Strategy for Elimination of Nucleic Acid Contamination.

Ineffective contamination prevention strategies significantly limit the deployment of nucleic acid testing in point-of-care (POC) settings. We herein propose a benzonase-based closed-tube strategy that degrades amplification products of nucleic acid. During the amplification reaction in the tube, Benzonase is kept on the inner surface of the tube cap.

SegR3D: A Multi-Target 3D Visualization System for Realistic Volume Rendering of Meningiomas.

Meningiomas are the most common primary intracranial tumors in adults. For most cases, surgical resection is effective in mitigating recurrence risk. Accurate visualization of meningiomas helps radiologists assess the distribution and volume of the tumor within the brain while assisting neurosurgeons in preoperative planning.

CAP-Net: Carotid Artery Plaque Segmentation System Based on Computed Tomography Angiography.

Rationale And Objectives: Diagnosis of carotid plaques from head and neck CT angiography (CTA) scans is typically time-consuming and labor-intensive, leading to limited studies and unpleasant results in this area. The objective of this study is to develop a deep-learning-based model for detection and segmentation of carotid plaques using CTA images.

Materials And Methods: CTA images from 1061 patients (765 male; 296 female) with 4048 carotid plaques were included and split into a 75% training-validation set and a 25% independent test set.

Oriented Antibody Functionalized Plasmonic Yolk-Shell-Satellite Nanostructures for Ultrasensitive SERS-Based Lateral Flow Immunoassay.

Conventional gold nanoparticle (AuNP)-based lateral flow immunoassays (LFIAs) for detecting matrix metalloproteinase-9 (MMP-9), a biomarker associated with dry eye disease and cancer, often suffer from limited sensitivity. To address this, we developed an ultrasensitive surface-enhanced Raman scattering (SERS)-based LFIA using plasmonic yolk-shell-satellite nanostructures (YSSNs) functionalized with bifunctional 4-mercaptophenylboronic acid (MPBA) as SERS tags. The YSSNs, composed of a gold nanorod core enclosed by a gold nanocage and decorated with satellite nanoparticles, provided strong SERS enhancement.

A novel colorimetric immunosensor based on silk cocoon membrane-integrated ELISA for treponemal antibody detection.

The global rise in syphilis incidence and the challenges of early diagnosis highlight the urgent need for convenient diagnostic tools. In this study, we present a colorimetric immunosensor for the detection of antibodies (TP-Ab) using a natural silk cocoon membrane (SCM) as the substrate, based on the principle of enzyme-linked immunosorbent assay (ELISA). The TP monoclonal antibody 3H12 and the chimera antigen TP99, comprising epitopes TP15, TP17, and TP47, were sequentially coupled to functionalized SCM to form the SCM-based TP-Ab immunosensor.