DNA Triangular Pyramid Frustum Supported miR-21 Assay with Duplex-Specific Nuclease-Assisted Target Recycling.

MicroRNAs (miRNAs) have the potential to be applied as effective biomarkers for early diagnosis of cancers. Electrochemical techniques exhibit advantages such as high sensitivity and ease of miniaturization. However, electrode interface perturbations may hinder electrochemical responses.

Polydopamine Powered Droplet Electricity Generator for Protein Assay with CRISPR/Cas Enabled Amplification.

Protein function investigation and clinical assay are fundamental to modern biology and medical diagnostics. Flap endonuclease 1 (FEN1), a key enzyme in DNA replication and repair, plays a critical role in the progression of many diseases. Taking FEN1 as an example, we present a novel protein detection platform combining triboelectric nanogenerator (TENG) and CRISPR/Cas technologies.

Intramolecular DNA Wheel Construction for Highly Sensitive Electrochemical Detection of miRNA.

Accurate and reliable quantification of disease-related biomolecules is essential for clinical diagnosis. In this study, a novel electrochemical approach is developed based on a target triggered DNA nanostructural switch from a hairpin dimer to a double-stranded wheel. During the process, electrochemical species get closer to the electrode interface, and the multiple intramolecular strand displacements are beneficial to low abundant target analysis.

Electrochemical Quantification of miRNA Based on Strain-Promoted Azide-Alkyne Cycloaddition Ligated Tetrahedral DNA Nanotags.

Highly sensitive and accurate detection of disease biomarkers is of great importance for diagnosis, staging, and treatment of certain diseases. Herein, we report a novel electrochemical method for the quantification of miRNA biomarkers with DNA tetrahedrons as the signal reporters. Upon the initiation of DNA hairpin opening by miRNA at the electrode interface, the hidden click reaction group is exposed for the bioconjugation with a tetrahedral DNA nanostructure, which carries multiple electrochemical species.

Liquid-Solid Triboelectric Nanogenerator-Based DNA Barcode Detection Biosensor for Species Identification.

DNA barcode detection method is widely applied for species identification, which is imperative to evaluate the effect of human economic activities on the biodiversity of ecosystem. However, the wide utilization of existing detection biosensors is limited by bulky and expensive instruments, such as Raman spectroscopy and electrochemical station. Herein, a liquid-solid triboelectric nanogenerator (TENG)-based DNA barcode detection biosensor is proposed, which consists of water flow, fluid channel, and PDMS film attached by specifically designed capture probe.

Co-toxicity and co-contamination remediation of polycyclic aromatic hydrocarbons and heavy metals: Research progress and future perspectives.

The combined pollution of polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs) has attracted wide attention due to their high toxicity, mutagenicity, carcinogenicity and teratogenicity. A thorough understanding of the progress of the relevant studies about their co-toxicity and co-contamination remediation is of great importance to prevent environmental risk and develop new efficient remediation methods. This paper summarized the factors resulting in different co-toxic effects, the interaction mechanism influencing co-toxicity and the development of remediation technologies for the co-contamination.

CD36 deletion prevents white matter injury by modulating microglia polarization through the Traf5-MAPK signal pathway.

Background: White matter injury (WMI) represents a significant etiological factor contributing to neurological impairment subsequent to Traumatic Brain Injury (TBI). CD36 receptors are recognized as pivotal participants in the pathogenesis of neurological disorders, including stroke and spinal cord injury. Furthermore, dynamic fluctuations in the phenotypic polarization of microglial cells have been intimately associated with the regenerative processes within the injured tissue following TBI.

Berberine and chlorogenic acid-assembled nanoparticles for highly efficient inhibition of multidrug-resistant Staphylococcus aureus.

Due to the bacteria resistant to various first-line antibiotics, it is urgent to develop efficient antibiotic alternatives and formulate multidimensional strategies. Herein, supramolecular Chinese medicine nanoparticles are synthesized by self-assembly of berberine (BBR) and chlorogenic acid (CGA), which exhibit higher inhibitory effect against Staphylococcus aureus and multidrug-resistant Staphylococcus aureus (MRSA) than ampicillin, oxacillin, BBR, CGA, as well as mixture of BBR and CGA (minimum inhibitory concentration, MIC = 1.5 µM).

Simultaneous adsorption and biodegradation of oxytetracycline in wastewater by Mycolicibacterium sp. immobilized on magnetic biochar.

Due to the adverse effects of long-term oxytetracycline (OTC) residues in aquatic environments, an effective treatment is urgently needed. Immobilized microbial technology has been widely explored in the treatment of various organic pollutants in aquatic environments with its excellent environmental adaptability. Nevertheless, studies on its application in the removal of antibiotics are relatively scarce and not in sufficient depth.

Neutrophil extracellular traps facilitate sympathetic hyperactivity by polarizing microglia toward M1 phenotype after traumatic brain injury.

Traumatic brain injury (TBI), particularly diffuse axonal injury (DAI), often results in sympathetic hyperactivity, which can exacerbate the prognosis of TBI patients. A key component of this process is the role of neutrophils in causing neuroinflammation after TBI by forming neutrophil extracellular traps (NETs), but the connection between NETs and sympathetic excitation following TBI remains unclear. Utilizing a DAI rat model, the current investigation examined the role of NETs and the HMGB1/JNK/AP1 signaling pathway in this process.

Structure-performance correlation guided cerium-based metal-organic frameworks: Superior adsorbents for fluoride removal in water.

Fluoride in the hydrosphere exceeds the standard, which could be critically hazardous to human health and the natural environment. The adsorption method is a mature and effective way to remove pollutants in water, including fluoride. In this study, we synthesized three kinds of cerium-based metal-organic frameworks (Ce-MOFs) with different structures and properties by modulating the organic ligands (i.

Trends in Adherence to Recommended Physical Activity and Its Association with Mortality and Disease Progression among US Adults with Chronic Kidney Disease.

Introduction: This study aimed to examine the trends in adherence to the Physical Activity Guidelines (PAG) for aerobic activity and sedentary time and their effects on mortality and disease progression among US adults with chronic kidney disease (CKD).

Methods: We studied individuals from the National Health and Nutrition Examination Survey 2007-08 to 2017-18 with a mortality file in 2015. Multivariate regression models were used to evaluate the association between adherence to PAG and sedentary time with mortality, estimated glomerular filtration rate (eGFR), and urine albumin-to-creatinine ratio.

Trends in adherence to recommended physical activity and its effects on cardiometabolic markers in US adults with pre-diabetes.

Introduction: This study aimed to examine the trends in adherence to Physical Activity Guidelines for Americans (PAG) as well as the association between them and cardiometabolic risk factors among US adults with pre-diabetes.

Research Design And Methods: This study included 6734 participants who were diagnosed with pre-diabetes from the National Health and Nutrition Examination Survey 2007-2008 to 2017-2018. The logistic regression model and linear regression model were used to test the trends in adherence to PAG.

Methyladenosine Modification in RNAs: From Regulatory Roles to Therapeutic Implications in Cancer.

Methyladenosine modifications are the most abundant RNA modifications, including N6-methyladenosine (mA), N1-methyladenosine (mA), and 2'-O-methyladenosine (mA). As reversible epigenetic modifications, methyladenosine modifications in eukaryotic RNAs are not invariable. Drastic alterations of mA are found in a variety of diseases, including cancers.

NETs Lead to Sympathetic Hyperactivity After Traumatic Brain Injury Through the LL37-Hippo/MST1 Pathway.

Paroxysmal sympathetic hyperactivity (PSH) is one of the important reasons for the high mortality and morbidity of traumatic brain injury (TBI). We aim to explore the role of the neutrophil extracellular traps (NETs) in the pathogenesis of sympathetic hyperexcitability after TBI and the underlying mechanisms, providing evidence for clinical treatment. Enzyme-linked immunosorbent assay was used to assess the plasma metanephrine and normetanephrine levels which represented the variation of the sympathetic system after TBI with rat diffuse axonal injury (DAI) model.

The Alteration of M6A-Tagged Transcript Profiles in the Retina of Rats After Traumatic Optic Neuropathy.

Messager RNA (mRNA) can be modified in a variety of ways, among which the modification of N6-methyladenosine (m6A) is one of the most common ones. Recent studies have found that the m6A modification in mRNA could functionally regulate the splicing, localization, translation, and stability of mRNA, which might be closely related to multiple diseases. However, the roles of m6A modification in traumatic optic neuropathy (TON) are unknown.

The emerging roles of circular RNAs in CNS injuries.

With high morbidity and mortality worldwide, injuries to the central nervous system (CNS) usually result in devastating consequences. However, the underlying mechanisms have not been fully understood and current therapies are still limited. Circular RNA (circRNA) is a novel type of endogenous noncoding RNAs, characterized by covalently closed annular structure.

Management of a Steel Bar Injury Penetrating the Head and Neck: A Case Report and Review of the Literature.

Background: Nonmissile penetrating injuries to the head and neck caused by a steel bar are rare, and a standard management strategy is lacking.

Case Description: A 42-year-old woman sustained a steel bar injury with penetration of the head and neck. Computed tomography and three-dimensional reconstruction were performed for preoperative evaluation.

Peak Neutrophil-to-Lymphocyte Ratio Correlates with Clinical Outcomes in Patients with Severe Traumatic Brain Injury.

Background: Studies suggested that the neutrophil-to-lymphocyte ratio (NLR) was associated with unfavorable outcomes in different diseases such as intracerebral hemorrhage, cardiovascular problem, cancer, and severe traumatic brain injury (sTBI). We aimed to evaluate the relationship between peak NLR and 1-year outcomes in patients with sTBI.

Methods: We retrospectively reviewed the clinical data of patients with sTBI who were treated in our department between January 2013 and January 2017.