Homogeneous Electrochemical Enzyme-Linked Immunosorbent Assay Strategy Based On pH-Mediated Redox Potential Regulation.

Enzyme-linked immunosorbent assay (ELISA) is widely recognized as the gold standard for protein detection. However, its reliance on expensive and bulky optical instruments limits its use in point-of-care and resource-limited settings. Electrochemical technique emerges as a promising alternative due to its low cost, portability, and simple instrumentation.

Impact assessment of the ophthalmology discipline construction project at Tianjin Medical University Eye Hospital.

Objectives: Research impact is an emerging measure of research achievement alongside traditional academic outputs such as publications. We present the results of applying the Framework to Assess the Impact from Translational Health Research (FAIT) to the ophthalmology discipline construction project at Tianjin Medical University Eye Hospital.

Methods: Data were gathered via in-depth interviews and document analysis to report on the three major FAIT methods: the Payback Framework, an assessment of costs against monetized outcomes and a narrative account of the impact generated from ophthalmology discipline construction project.

Influence of Soil Depth and Land Use Type on the Diversity of and Metabolic Restriction in the Soil Microbial Community of a Forest-Grass Ecotone.

Revealing soil microbial diversity and metabolic limitations in different land uses and soil depths is essential to understanding the regulation processes of soil nutrients. Here, bacterial and fungal microbial diversity and metabolic restriction in the 0-50 cm soil layers of four land uses, namely farmland, grassland, secondary forest, and -planted forest in the mountainous forest-grass ecotone of northern China, were determined. The results showed that soil microbial diversity in farmland was the lowest.

Benzonase-Based Closed-Tube DNA Degradation Strategy for Elimination of Nucleic Acid Contamination.

Ineffective contamination prevention strategies significantly limit the deployment of nucleic acid testing in point-of-care (POC) settings. We herein propose a benzonase-based closed-tube strategy that degrades amplification products of nucleic acid. During the amplification reaction in the tube, Benzonase is kept on the inner surface of the tube cap.

The Role of Multiparametric MRI Radiomics for Preoperative Prediction of Axillary Lymph Node Metastasis in Patients With Invasive Breast Cancer: A Comparative Study.

Background: The predictive value of different MRI sequences for axillary lymph node metastasis (ALNM) in patients with invasive breast cancer remains unclear. This study compared the performance of radiomics models based on individual and combined MRI sequences for the preoperative prediction of ALNM and evaluated the clinical application value of the optimal model.

Methods: This retrospective study included 454 patients (mean ± SD age 50.

The impact of hyperventilation on seizure duration following electroconvulsive therapy: a systematic review and meta-analysis of randomized trials.

Background: Electroconvulsive therapy (ECT) is a treatment used for severe psychiatric conditions that involves the induction of seizures under general anesthesia. It has been proposed that hyperventilation, which is the rapid breathing either voluntary or induced to lower carbon dioxide levels, might have an impact on the effectiveness of ECT. This systematic review and meta-analysis aimed to consolidate the current evidence on how hyperventilation may alter the course and outcomes of ECT by potentially affecting seizure duration and the resultant therapeutic outcomes, and assess its implications for both safety and potential therapeutic outcomes for patients.

Cell Surface-Confined DNAzyme Coordination-Facilitated Logical Engineering for Precise Cell Identification.

DNA logical processing, which employs DNA as a building block to perform logic operations, attracts considerable attention in biomedical applications. Herein, a new DNA logical processing strategy is explored for selective cell engineering and to develop a feasible technology for precise cell identification. Specifically, this cell identification technology accomplishes logical engineering through the employment of cell surface-confined DNAzyme coordination, which not only enables the labeling of versatile DNA probes at specific cells but also avoids false-positive outputs caused by the neighboring non-target cells.

Sequential Labeling-Assisted Precise and Multitarget Analysis of Surface Proteins on Extracellular Vesicles.

Analysis of multiple surface proteins on extracellular vesicles (EVs) can reveal biological characteristics and potential therapeutic targets of cancer, particularly in highly heterogeneous breast cancer. However, due to the limited surface area of EVs, spatial hindrance remains a challenge for multiprotein assessment. Here, we present a sequential labeling-assisted electrochemical method for the precise and multitarget analysis of surface proteins on EVs, using breast cancer-related epidermal growth factor receptor and programmed death ligand-1 as examples.

Activating Organic Electrode for Zinc Batteries via Adjusting Solvation Structure of Zn Ions.

Zinc-organic batteries, combining the low cost and high capacity of Zn anodes with the tunable and sustainable properties of organic cathodes, have garnered significant attention. Herein, we present a zinc-organic battery featuring a poly(benzoquinonyl sulfide) (PBQS) cathode, a Zn anode, and an N,N-dimethylformamide (DMF)-based electrolyte, which delivers a high capacity (200 mAh g), excellent rate capability, and an ultra-long cycle life (10,000 cycles) when tested with a low PBQS loading (2 mg cm). The charge storage mechanism in the PBQS cathode involves solvated Zn adsorption and consequent Zn coordination with PBQS companied by de-solvation process, as confirmed by in situ FT-IR analysis.

Strategies and Prospects for Engineering a Stable Zn Metal Battery: Cathode, Anode, and Electrolyte Perspectives.

ConspectusZinc metal batteries (ZMBs) appear to be promising candidates to replace lithium-ion batteries owing to their higher safety and lower cost. Moreover, natural reserves of Zn are abundant, being approximately 300 times greater than those of Li. However, there are some typical issues impeding the wide application of ZMBs.

Anti-Müllerian hormone regulates ovarian granulosa cell growth in PCOS rats through SMAD4.

Objective: Polycystic ovary syndrome (PCOS) is a diverse condition with an unknown cause. The precise mechanism underlying ovulatory abnormalities in PCOS remains unclear. It is widely believed that malfunction of granulosa cells is the primary factor contributing to aberrant follicular formation in PCOS.

[Changes of soil microbial phospholipid fatty acid in soil profile under different land use types].

We elucidated the changes of soil microbial biomass and community structure in soil profiles under four typical land use types (farmland, grassland, secondary forest and plantation)and across five soil layers (0-10, 10-20, 20-30, 30-40, 40-50 cm) in the northern mountainous region of Hebei Province. We measured soil microbial biomass by phospholipid fatty acid (PLFA) method, and investigated the effects of land use and soil depth on soil microbial biomass and community structure by variance analysis, correlation analysis and redundancy analysis. The results showed that soil water content, bulk density, and organic carbon content of farmland differed significantly from other land use types.

Degradable Radical Polymer Cathode for Lithium Battery with Long-Term Cycling Capability.

Polymer-based organic electrodes for rechargeable batteries are attractive due to their design flexibility, sustainability, and environmental compatibility. Unfortunately, waste management of conventional polymer materials typically involves incineration, which emits greenhouse gases. Consequently, degradable polymers should be ideal candidates for future green batteries.

The Impact of Exposure to Iodine and Fluorine in Drinking Water on Thyroid Health and Intelligence in School-Age Children: A Cross-Sectional Investigation.

Iodine and fluorine, as halogen elements, are often coexisting in water environments, with nearly 200 million people suffering from fluorosis globally, and, in 11 countries and territories, adolescents have iodine intakes higher than that required for the prevention of iodine deficiency disorders. It has been suggested that excess iodine and/or fluorine can affect thyroid health and intellectual development, especially in children, but their combined effect has been less studied in this population. This study investigated 399 school-age children in Tianjin, China, collected drinking water samples from areas where the school-age children lived, and grouped the respondents according to iodine and fluorine levels.

Nonflammable Fluorinated Electrolyte Realizing High Voltage Anode-Free Zn Dual-Ion Batteries.

Due to the low decomposition potential of HO and its corrosive effect to Zn foil, the Zn metal battery with aqueous electrolytes operates within a narrow electrochemical window and exhibits low anode utilization ratio. Fluorinated carbonate ester, exhibiting low highest occupied molecular orbital (HOMO) energy level, is suitable for constructing high-voltage batteries, yet its application in Zn metal battery has been scarcely explored. Herein, we propose an electrolyte based on fluorinated solvents and ethoxy (pentafluoro) cyclotriphosphazene (PFPN) additive, which exhibits a high decomposition voltage of 2.

Triglyceride-glucose index-A novel metabolism disorder biomarker as a promising indicator for predicting postoperative 30-day infections in elderly patients undergoing gastrointestinal-related abdominal and pelvic surgery.

Background: The triglyceride-glucose index, a reliable surrogate biomarker of insulin resistance, has been reported to be associated with cardiovascular events and atherosclerosis. However, few studies have investigated the association of the triglyceride-glucose index with postoperative infections. This study aimed to study the clinical risk values of the preoperative triglyceride-glucose index in postoperative infection complications in elderly patients undergoing gastrointestinal-related abdominal and pelvic surgery.

Colorimetric detection of furin based on enhanced catalytic activity of G-quadruplex/hemin DNAzyme.

Background: Rapid and sensitive colorimetric detection methods are crucial for diseases diagnosis, particularly those involving proteases like furin, which are implicated in various conditions, including cancer. Traditional detection methods for furin suffer from limitations in sensitivity and practicality for on-site detection, motivating the development of novel detection strategies. Therefore, developing a simple, enzyme-free, and rapid colorimetric analysis method with high sensitivity for furin detection is imperative.

Harnessing chemical functionality of xylan hemicellulose towards carbohydrate polymer-based pH/magnetic dual-responsive nanocomposite hydrogel for drug delivery.

This study reports a pH/magnetic dual-responsive hemicellulose-based nanocomposite hydrogel with nearly 100 % carbohydrate polymer-based and biodegradable polymer compositions for drug delivery. We synthesized pure FeO magnetic nanoparticles (FeO MNPs) using a co-precipitation method, then engineering xylan hemicellulose (XH), acrylic acid, poly(ethylene glycol) diacrylate, and FeO to synthesize the pH/magnetic dual-responsive hydrogel (FeO@XH-Gel), through graft polymerization on XH with in-situ doping FeO MNPs initiated by the ammonium persulfate/tetramethylethylenediamine redox system. Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (H NMR), X-ray diffractometry (XRD), scanning electron microscopy and energy dispersive spectrometer (SEM-EDS), high-resolution transmission electron microscopy (HRTEM), Brunauer-Emmett-Teller (BET), swelling gravimetric analysis, vibrating sample magnetometer (VSM) were employed to analyze the hydrogel's chemical structures, morphologies, pH-responsive behaviors, and magnetic responsiveness characteristics, mechanical and rheological properties, as well as cytotoxicity and biodegradability.

Coeliac disease and postpartum depression: are they linked? A two-sample Mendelian randomization study.

Background: To explore the potential causal associations between coeliac disease(CD) and postpartum depression(PPD) by using two-sample Mendelian randomization(MR) analysis.

Methods: The IEU OPEN GWAS project was utilized to identify genetic loci strongly associated with CD as instrumental variables (IVs), and MR analysis was performed using inverse variance weighting(IVW), weighted median, weighted model, and MR-Egger. MR analyses were used to examine whether there was a link between CD and PPD, with an OR and 95% CI.

Endothelial lincRNA-p21 alleviates cerebral ischemia/reperfusion injury by maintaining blood-brain barrier integrity.

Blood-brain barrier (BBB) disruption is increasingly recognized as an early contributor to the pathophysiology of cerebral ischemia/reperfusion (I/R) injury, and is also a key event in triggering secondary damage to the central nervous system. Recently, long non-coding RNA (lncRNA) have been found to be associated with ischemic stroke. However, the roles of lncRNA in BBB homeostasis remain largely unknown.

Synthesis of a COF-on-MOF hybrid nanomaterial for enhanced colorimetric biosensing.

The construction of hybrid materials is significant for the exploration of functionalities in colorimetric biosensing due to its structural designability and synergy effects. In this work, a COF-on-MOF hybrid nanomaterial has been newly synthesized for colorimetric biosensing. Experimental results reveal that on-surface synthesis of COF on MOF brings nanoscale proximity between COF and MOF, which exhibits more than two folds of peroxidase-like activity as compared to single Fe-MOF.

Carbonate Ester-Based Electrolyte Enabling Rechargeable Zn Battery to Achieve High Voltage and High Zn Utilization.

Owing to the high HO activity, the aqueous electrolyte in the Zn battery exhibits a narrow electrochemical window and inevitable hydrogen evolution reaction, limiting the anode utilization ratio and performance at high voltage. Carbonate ester, the well-developed electrolyte solvent in Li-ion batteries, exhibits aprotic properties and high anodic stability. However, its use in Zn metal batteries is limited due to the low solubility of Zn salts in carbonate esters.