Investigating the Potential Effects of 6PPDQ on Clear Cell Renal Cell Carcinoma via Network Toxicology and Molecular Docking Analysis With In Vitro Experimental Validation.

N-(1,3-Dimethylbutyl)-N'-phenyl-p-phenylenediamine-quinone (6PPDQ), as a recently identified environmental toxicant, has garnered significant attention because of its widespread detection in ecosystems and human habitats. Emerging evidence highlights its potential detrimental effects on various organs. However, its carcinogenic potential remains poorly understood, particularly its role in clear cell renal cell carcinoma (ccRCC).

FoxO3a: capture the bond between magnesium and diabetic kidney disease.

Hyperglycemia in Diabetic Kidney Disease (DKD) induces excessive accumulation of reactive oxygen species (ROS) through various pathways, leading to oxidative stress, ferroptosis, and mitochondrial dysfunction, which collectively contribute to kidney damage. Currently, the treatment of DKD remains a significant challenge. Magnesium, an essential mineral, has emerged as a promising therapeutic agent for DKD due to its anti-inflammatory and antioxidant properties.

Exploring potential associations and biomarkers linked polycystic ovarian syndrome with atherosclerosis via comprehensive bioinformatics analysis, machine learning, and animal experiments.

Polycystic ovary syndrome (PCOS), a common endocrine condition affecting multiple systems, is tied to atherosclerosis (AS) progression among reproductive-aged women. The present study aimed to explore the underlying associations and uncover potential biological indicators for PCOS complicated with AS. Gene expression datasets for PCOS and AS were obtained from Gene Expression Omnibus (GEO).

Post-PKS Tailoring of Phoslactomycins Involving Two Cytochrome P450s.

Combining and approaches, we elucidated the phoslactomycin post-PKS tailoring pathway in . Gene inactivation of - revealed seven phoslactomycin derivatives (four novel). Two cytochrome P450s were identified: PnT3 (mediating dual-site, multistep C-8/C-25 oxidation) and PnT7 (catalyzing C-18 hydroxylation).

Modulation of senescence-related genes by the transcription factor PlPLATZ5 prolongs the lifespan of Paeonia lactiflora petals.

Four PLATZ family members were identified in the petals of herbaceous peony (Paeonia lactiflora), all of which contained two typical domains, BBOX and PLATZ. Notably, PlPLATZ5 was up-regulated during the transition from flowering to senescence, exhibited high expression in floral organs, and was localized to the nucleus, as revealed by subcellular localization analysis. Transient overexpression of PlPLATZ5 in P.

Pre-silencing of TNF-α by targeted siRNA delivery mitigates glucocorticoid resistance of dexamethasone in rheumatoid arthritis.

Rheumatoid arthritis (RA) is a chronic, progressive inflammatory autoimmune disease marked by relentless synovial inflammation and joint destruction, for which long-term remission remains challenging. Although dexamethasone (DEX) is commonly employed to rapidly control disease activity, its therapeutic effectiveness is often undermined by the development of glucocorticoid resistance (GCR) and cumulative systemic toxicities. Recent insights suggest that TNF-α-driven inflammation not only perpetuates joint pathology but also sustains a molecular landscape that favors GCR, underscoring an urgent need for therapeutic strategies that jointly target inflammatory signaling and steroid sensitivity.

Synthesis and Preclinical Evaluation of Dual-Targeted Molecular Tracer for Positron Emission Tomography Imaging of Programmed Cell Death Ligand‑1 and Integrin αβ.

Dual-targeting strategies have shown considerable potential in improving the imaging contrast and specificity toward tumor tissues. In this study, we synthesized a dual-targeted tracer, , which synergistically targeted programmed cell death ligand-1 (PD-L1) and integrin αβ. The tracer was produced with high radiochemical purity (>95%) and radiochemical yield (>95%).

Systematic identification of heat-responsive VQ genes and functional characterization of the TaVQ27-5A-TaWRKY51L-3A module in thermotolerance regulation in wheat (Triticum aestivum L.).

Valine-glutamine (VQ) proteins are plant-specific transcriptional cofactors that play crucial roles in plant growth, development, and stress responses. Heat stress (HS), one of the most detrimental abiotic stresses, severely impairs wheat growth, development, quality, and yield. Despite their importance in other plants, the role of VQ proteins in wheat's response to HS remains unexplored.

Transfer dynamics of extracellular antibiotic resistance genes during antibiotic and municipal wastewater treatment: contribution analysis and host identification.

The occurrence, transfer contribution, and potential hosts of intracellular and extracellular antibiotic resistance genes (ARGs) in an antibiotic and a municipal wastewater treatment plant (WTP) were comprehensively evaluated. Free-extracellular ARGs (eARGs) were dominant in effluents (59.4-79.

ALKBH1 drives tumorigenesis and drug resistance via tRNA decoding reprogramming and codon-biased translation.

Cancer cells utilize codon-biased translation to fuel tumorigenesis and drug resistance, but underlying mechanisms remain poorly understood. Here, we show ALKBH1 is overexpressed in acute myeloid leukemia (AML) and essential for leukemia stem/initiating cell (LSC/LIC) self-renewal and AML development/maintenance, whereas dispensable for normal hematopoiesis. ALKBH1 enhances mitochondrial assembly/function and oxidative phosphorylation (OXPHOS), crucial for AML survival/proliferation and resistance to venetoclax, a potent BCL2 inhibitor and widely-used first-line targeted therapy for AML in clinic.

[Corrigendum] Cerebral ischemic post‑conditioning induces autophagy inhibition and a HMGB1 secretion attenuation feedback loop to protect against ischemia reperfusion injury in an oxygen glucose deprivation cellular model.

Subsequently to the publication of the above paper, the authors drew to the Editor's attention that matching HMGB1 bands featured in Figs. 4 and 5 had inadvertently been included in these figures twice; moreover, the GAPDH western blot controls featured in Fig. 3 had also been selected incorrectly.

Bioink design for organ-scale projection-based 3D bioprinting.

Projection-based three-dimensional bioprinting offers an approach for manufacturing biomimetic tissues with complex spatial structures and bioactivity, presenting potential for creating implantable organs or organoids to test drug response. Nevertheless, the extended printing times required for organ-scale manufacturing represents a challenge. Here we provide step-by-step instructions to manufacture organ-scale structures using bioinks while preserving high bioactivity.

L-shaped nonlinear relationship between magnesium intake from diet and supplements and the risk of diabetic nephropathy: a cross-sectional study.

Background And Objectives: Given the ongoing controversy regarding the relationship between magnesium and diabetic nephropathy (DN), this study systematically evaluate the association between total magnesium intake from both dietary and supplemental sources and the risk of DN, and further explore its potential nonlinear dose-response pattern and threshold effect.

Methods: Data were from the National Health and Nutrition Examination Survey 2007-2018. A multi-step analytical strategy was adopted: (1) confounders were selected using variance inflation factor and Boruta feature selection algorithm; (2) weighted multivariable logistic regression assessed the association between magnesium intake and DN; (3) restricted cubic splines (RCS), generalized additive models (GAM), and curve fitting were used to evaluate nonlinear dose-response trends; (4) piecewise regression identified potential thresholds; (5) subgroup analyses examined interactions across age, gender, BMI, hypertension, and cardiovascular disease.

Targeting STING alleviates pyroptosis of bladder epithelial cells and ameliorates bladder fibrosis in neurogenic bladder.

Neurogenic bladder (NB) is a common urinary system disease in children and often accompanied by bladder inflammation and fibrosis. Currently, NB has very few treatment options. Stimulator of interferon genes (STING) links immune response with inflammatory processes and is associated with the development of diverse fibrotic diseases.

Identification of candidate genes for grain size in barley through combined GWAS and transcriptome analysis.

Barley (Hordeum vulgare L.) is an important cereal crop with diverse uses, including animal feed, beer brewing, and food production. Grain size plays a crucial role in determining grain weight and quality, which is one of the key breeding objectives in response to market and industry demands.

Nuclear phylogenomics provide evidence to clarify key morphological evolution and whole-genome duplication across rosids.

Rosids, comprising 90,000-120,000 species, form a large clade of angiosperms, including extensively studied families with many economically and scientifically important plants. They are also ecologically important, dominating many temperate and tropical ecosystems. Great progress in understanding rosid phylogenetic relationships has facilitated evolutionary studies, but phylogenetic uncertainties remain.

Engineering Safety-Oriented Blasting-Induced Seismic Wave Signal Processing: An EMD Endpoint Suppression Method Based on Multi-Scale Feature.

Blasting-induced seismic waves are typically nonlinear and non-stationary signals. The EMD-Hilbert transform is commonly used for time-frequency analysis of such signals. However, during the empirical mode decomposition (EMD) processing of blasting-induced seismic waves, endpoint effects occur, resulting in varying degrees of divergence in the obtained intrinsic mode function (IMF) components at both ends.

Profiling of extracellular antibiotic resistance genes during antibiotic production wastewater treatment processes: Occurrence, transformation dynamics, and potential hosts.

The propagation of antibiotic resistance genes (ARGs) in both intracellular and extracellular forms during wastewater treatment processes has garnered increasing attention. Antibiotic production wastewater (APW) contains high concentrations of antibiotics and organics known to induce the transformation of extracellular ARGs (eARGs) and potentially facilitate antibiotic resistance transmission. In this study, the presence, transformation dynamics, and potential hosts of eARGs in a full-scale APW treatment plant (APWTP) were investigated.

Electroencephalography-based psychological and physiological effects of winter virtual forest trail landscapes on youth.

In the context of escalating urbanization and modernization, urban residents are facing a progressive rise in stress levels, particularly during winter when severe cold and limited daylight hours intensify psychological strain and physical fatigue. Despite the acknowledged health benefits of brief exposure to natural settings, research on the restorative effects of virtual winter forest settings remains limited. This study undertook the creation of immersive winter forest trail landscapes using virtual technology, generating six distinct audiovisual configurations by manipulating variables such as Evergreen Tree Density (ETD) and Event Ambient Sound (EAS).

PRMT6 Inhibits Malignant Progression of Melanoma by Antagonizing Trimethylation of Histone H3K4 to Downregulate ALDH1A1 Levels.

Protein arginine methyltransferase 6 (PRMT6), a member of the PRMT family capable of self-arginine methylation, is down-regulated in melanoma, but the specific mechanism is still unclear. Our work demonstrated that PRMT6 overexpression significantly inhibited the ability of melanoma cells to proliferate, tumorigenicity, migration, and invasion, whereas PRMT6 knockdown rescued these malignant behaviors of melanoma cells. Mechanistically, we identified aldehyde dehydrogenase 1A1 (ALDH1A1) as a critical downstream target of PRMT6.

Attention-Deficit Hyperactivity Disorder and Childhood Allergy: Evidence From Mendelian Randomization.

Background: The association between attention-deficit hyperactivity disorder (ADHD) and allergic diseases in children has been reported in observational studies, yet whether the relationship reflects causality remains unclear.

Methods: To address this, we performed a two-sample bidirectional Mendelian randomization (MR) analysis using genetic instrumental variables associated with ADHD (N = 38 691) and childhood allergy (N = 5337) from the largest available genome-wide association studies. Inverse-variance weighted was used as the primary estimate, whereas Cochran's Q test and MR-Egger regression were used to detect heterogeneity and pleiotropy.

Microglial insulin resistance drives neurodegeneration.

Brain insulin resistance (BIR) contributes to neurodegenerative diseases such as Alzheimer's disease (AD). Recently, Chen et al. revealed that microglial insulin signaling loss drives neuroinflammation and amyloid-β (Aβ) accumulation, promoting AD progression.

Effectiveness of Acupotomy Subcision and Nanofat Grafting in Depressed Scars Treatment.

Purpose: The purpose of this study was to investigate the effectiveness of the combined treatment of acupotomy subcision and Nanofat grafting in depressed scars.

Methods: A single-center retrospective analysis was performed on 32 patients with moderate to severe depressed facial scars treated between November 2020 and April 2023. The treatment involved acupotomy subcutaneous scrape, followed by Nanofat injection.

Insights into the molecular mechanisms of browning tolerance in luffa: a transcriptome and metabolome analysis.

Introduction: Enzymatic browning significantly affects the edible, nutritional, and commercial value of luffa. Investigating the expression and regulation of key enzyme genes involved in the browning process is crucial for understanding the molecular mechanisms underlying luffa browning.

Methods: Fruit samples were collected at 15 (S1), 20 (S2), and 45 days (S3) after flowering from two contrasting luffa varieties: the browning-sensitive Long-quan-yi (LQY) and the browning-tolerant Jiang-du (JD).