The public's perceptions and attitudes of male nursing postgraduate professional identity on Chinese social media: Qualitative study based on machine learning.

Background: Professional identity plays a critical role in the career development of male postgraduate nursing students, particularly in contexts where gender imbalance and social stereotypes persist.

Objective: This study explores how the clinical professional identity of male nursing postgraduates is perceived and constructed through social media discourse in China.

Design: A qualitative study using content analysis of social media discourse, supported by sentiment classification and clustering algorithms.

Heterogeneity in Phenotype and Early Metabolic Response to Lifestyle Interventions in Type 2 Diabetes in China Using a Tree-Like Representation.

Unlabelled: Deciphering the heterogeneity of type 2 diabetes in prognosis and treatment effect is essential. We used a novel dimensionality reduction approach to describe the type 2 diabetes phenotype continuum and visualize the difference in lifestyle intervention efficacy in Chinese patients. Based on 17,816 participants with newly diagnosed type 2 diabetes (aged ≥40 years) from a nationwide cohort, 12 key phenotypes were residualized for age and sex to construct a two-dimensional tree structure.

Stage-Resolved Phosphoproteomic Landscape of Mouse Spermiogenesis Reveals Key Kinase Signaling in Sperm Morphogenesis.

Spermiogenesis is the committed step of sperm production, during which spermatid cells undergo dramatic morphological transformations and transcriptional silencing. Post-translational modifications (PTMs), including phosphorylation, provide a level of protein function flexibility and play important roles in spermiogenesis. Dynamic protein phosphorylation profiles of spermatids are characterized across four different developing steps, and identified phosphorylation regulation of key proteins in spermiogenesis.

Targeting RAS-mutant cancer cells using a synthetic RAS-activated cancer killing system.

Despite being the most commonly mutated proteins in cancer, oncogenic RAS proteins remain largely untapped as pharmacological targets. Here, we report a synthetic cancer-killing platform, termed 'RAS-activated cancer killing (RACK)' system. Leveraging a transcriptional sensor designed to detect oncogenic RAS signals with high specificity, RACK achieves targeted identification and elimination of RAS-mutant cancer cells.

FoxO3a: capture the bond between magnesium and diabetic kidney disease.

Hyperglycemia in Diabetic Kidney Disease (DKD) induces excessive accumulation of reactive oxygen species (ROS) through various pathways, leading to oxidative stress, ferroptosis, and mitochondrial dysfunction, which collectively contribute to kidney damage. Currently, the treatment of DKD remains a significant challenge. Magnesium, an essential mineral, has emerged as a promising therapeutic agent for DKD due to its anti-inflammatory and antioxidant properties.

Multi-Omics and Molecular Docking Studies on Caffeine for Its Skin Rejuvenating Potentials.

: Caffeine (CA) exhibits promising reparative effects against UV-induced skin aging, but the specific mechanisms, including differences in gene and metabolite regulation and the involvement of signaling pathways, are still insufficiently elucidated. : This study is on the repairing capability of CA to ultraviolet (UV)-induced skin aging and explores the ferroptosis pathway through in vitro cell experiments, a UV-aged mouse skin model, and molecular docking. : CA enhanced the vitality and proliferation of HaCaT cells, delayed cell aging, reduced reactive oxygen species levels, increased mitochondrial membrane potential, and activated the peroxisome proliferator-activated receptor pathway, as well as repaired UVB-induced cytoskeletal disorders.

An Activation Likelihood Estimation Meta-Analysis of How Language Balance Impacts the Neural Basis of Bilingual Language Control.

Neurological networks involved in bilingual language control have been extensively investigated. Among the factors that influence bilingual language control, language balance has recently been proposed as a critical one. Nevertheless, it remains understudied how the neural basis of bilingual language control is affected by language balance.

Mechanism of RCD and the Role of Different Death Signaling Pathways in Cancer.

Cancer remains a significant global health challenge, with China being particularly affected because of its large population. Regulated cell death (RCD) mechanisms, including autophagy, apoptosis, necroptosis, pyroptosis, and ferroptosis, play complex roles in cancer development and progression. This review explores the dual roles of autophagy and apoptosis in cancer, highlighting their tumor-suppressive and tumor-promoting functions.

Spherical and fibrillar ovalbumin aggregates: Tailoring oleogel-based Pickering emulsions for improved curcumin bioavailability and anti-inflammatory effects.

The structural stability, rheological properties, and delivery potential of oleogel-based Pickering emulsions (OPEs) stabilized by ovalbumin (OVA) aggregates with different morphologies were systematically investigated. The OVA aggregates with spherical and fibrillar morphologies were prepared under controlled pH and thermal conditions. It was found that OVA aggregates with fibrillar morphology (OFA) exhibited higher surface hydrophobicity (1545 ± 6.

The pivotal regulatory role of the FEV-SLC7A11 axis in ferroptosis elucidates the anti-aging mechanism of β-sitosterol in a cross-species study.

Traditional Chinese medicine is a valuable source of bioactive compounds for combating aging. Among these, β-sitosterol (BS), a sterol extracted from , has attracted attention for its potent antioxidant, anti-inflammatory, and free radical scavenging properties. However, its precise anti-aging mechanism remains unclear.

CaOSC5-CaOSC7 module governs metabolic partitioning between asiaticoside and asiaticoside B in Centella asiatica.

Asiaticoside and asiaticoside B, bioactive triterpenoid saponins from Centella asiatica (C. asiatica), exhibit distinct structural, pharmacological, and accumulation profiles. Asiaticoside (ursane-type, α-amyrin-derived) possesses skin-lightening and wound-healing properties, whereas asiaticoside B (oleanane-type, β-amyrin-derived) displays antitumor activity.

Formation of neutrophil extracellular traps in the early stages exacerbate the healing process by regulating macrophage polarization in Achilles tendon-bone injury.

The influence of neutrophils and of neutrophil extracellular traps (NETs) on post-traumatic tendon-to-bone healing was studied in a murine model. The impact of neutrophil infiltration on macrophage polarization and peritendinous fibrosis in early-stage Achilles tendon injury is reported. Mice underwent Achilles tendon-bone injury and divided into four groups: sham operation, tendon injury (TI) treated with acetylcellulose (vehicle control), TI treated with a Protein arginine deiminase-4 (PAD4) inhibitor GSK484, and TI treated with a neutrophil elastase inhibitor Sivelestat.

Machine learning-based quantification of overall and internal ultrasound characteristics for diagnosing malignant partially cystic thyroid nodules.

Introduction: Partially cystic thyroid nodules (PCTNs) with malignant potential are frequently underestimated due to limited recognition of their sonographic characteristics.

Methods: This retrospective analysis included 486 PCTNs identified between March 2021 and September 2022. Machine learning (ML) was employed to quantitatively evaluate the overall ultrasound characteristics of the whole nodule as well as the internal ultrasound characteristics of its solid part.

Progress in the study of the emission characteristics of intermediate and semivolatile organic compounds from motor vehicles.

Previous studies have demonstrated that intermediate-volatility and semivolatile organic compounds (I/SVOCs) are important precursors of secondary organic aerosols. Motor vehicles are important sources of atmospheric I/SVOC emissions. In this paper, existing test methods for motor vehicle I/SVOCs are summarized, the advantages and disadvantages of various sampling methods and analytical techniques are compared, and the main factors influencing motor vehicle I/SVOC emissions are analyzed.

Dynamic changes of Ct values of N gene and ORF1ab genes and laboratory parameters in patients with COVID-19 caused by SARS-CoV-2 B.1, BA.2, and BA.5 variants and their correlation with clinical characteristics.

Objective: To characterize the dynamic patterns of ORF1ab and N gene Ct values in oropharyngeal swabs from COVID-19 patients infected with different SARS-CoV-2 variants and assess their clinical and laboratory correlations.

Methods: We conducted a retrospective cohort study of 259 COVID-19 patients hospitalized in Jilin Province between 2021-2023. Comparative analyses were performed on: (1) variant-specific Ct value trajectories for ORF1ab and N genes, (2) nucleic acid conversion times, and (3) longitudinal hematological and biochemical parameters.

Gene traffic mediated by transposable elements shaped the dynamic evolution of ancient sex chromosomes of varanid lizard.

Lizards usually exhibit frequent turnovers and a much greater diversity of sex determination mechanisms compared to birds and mammals, with the conserved ZW sex chromosomes of anguimorph lizards originating over 115 million years ago a seeming exception. We previously discovered in an anguimorph lizard Varanus acanthurus (Vac) whose entire chrW, but not chrZ is homologous to part of the chr2 by cytogenetic mapping, suggesting its complex history of sex chromosome evolution yet to be elucidated. To address this, we assemble a chromosome-level genome, and provide evidence that the Vac sex chromosome pair has undergone at least two times of recombination loss, producing a pattern of evolutionary strata like that of birds and mammals.

Weighted balanced truncation method for approximating kernel functions by exponentials.

Kernel approximation with exponentials is useful in many problems with convolution quadrature and particle interactions such as integral-differential equations, molecular dynamics, and machine learning. In this paper, we introduce a weighted balanced truncation method that significantly reduces the number of exponential terms required for an accurate representation of the kernel. This method shows great promise in approximating long-range kernels, achieving more than four digits of accuracy improvement for the Ewald splitting and inverse power kernels compared to classical balanced truncation.

Ultrasound-Assisted Targeted Therapy for Liver Fibrosis Using Rosiglitazone-Loaded Lipid Nanoparticles Functionalized with RGD Peptides.

Hepatic fibrosis is a progressive liver disease characterized by excessive accumulation of extracellular matrix (ECM) proteins, primarily collagen, in response to chronic liver injury. Despite the availability of treatment options, current therapies face significant challenges, including poor drug targeting and systemic toxicity. In this study, we developed a nanodrug delivery system, Rosiglitazone (RGZ)-loaded perfluoropropane (PFP)-based lipid nanoparticles (LNPs) functionalized with Arg-Gly-Asp (RGD) peptides (RGZ/PFP@LNP-RGD), for ultrasound-assisted targeted therapy of liver fibrosis.

Synthesis of Pyrrole-based Porous Organic Polymers for High Temperature Proton Conduction.

Porous organic polymers (POPs) with nitrogen-enriched structures are the type of materials with the advantages of porosity, excellent stability, and wide synthetic pathways, which possesses the great potential for application in the proton conduction. Herein, we report the synthesis of a novel type of pyrrole-based POPs that exhibit high surface areas and nitrogen-rich skeletons. The fabricated pyrrole-linked POPs render the high potential for the proton conducting application.

A Novel Peculiarity of TXNIP Reversing the Radioresistance of NPC and Inducing Ferroptosis by xCT-GSH-GPX4-ROS Axis.

Background: Radioresistance is a major problem in clinical treatment of nasopharyngeal carcinoma (NPC), and molecular mechanisms of radioresistance related to the antioxidant system are unknown. TXNIP (thioredoxin-interacting protein) is an endogenous inhibitor of thioredoxin. The system xCT/GSH/GPX4 axis is an important antioxidant system of ferroptosis in drug-resistant solid tumor therapy.

Cortical activation and functional connectivity in patients with chronic insomnia based on working memory tasks: a multi-channel near-infrared spectroscopy study.

Background: Working memory (WM) impairment has been found in patients with insomnia, and the frontotemporal region plays a central role in working memory. Therefore, changes in functional connectivity in the frontotemporal region may play an important role in WM impairment in patients of chronic insomnia, but little is known about the neural processes associated with WM dysfunction in patients with chronic insomnia. This study used functional near-infrared spectroscopy (fNIRS) to investigate differences in cortical activation and functional connectivity during a WM task between patients with chronic insomnia and healthy controls (HCs).

Synthesis of a Cage-like High Energy Density Compound with the 2-Azaadamantane Skeleton Containing Seven Nitro Groups at All-Bridge Positions.

The synthesis of azaadamantane-based energetic compounds with symmetrical structures and numerous explosophoric groups has always been a major challenge in the field of energetic materials. Herein, we report the synthesis of a fully bridged carbon-substituted 2-azaadamantane energetic compound with seven explosophoric groups. It exhibits a high crystal density (1.

MiR-195-5p regulates oxidative stress and aerobic metabolism by directly downregulating GLS2 in high glucose-induced human lens epithelial cells.

Objective: To investigated how miR-195-5p affects oxidative stress and modulates aerobic metabolism.

Materials And Methods: MiR-195-5p plus GLS2 mRNA was identified by conducting real-time quantitative polymerase chain reaction. Western blotting was conducted to determine GLS2 protein expression.

Association between living alone and frailty, and its gender disparity: a multicenter cross-sectional study in Chinese older adults undergoing hospitalisation.

Background: The association between living alone and frailty has not been systematically investigated in previous studies, among which inconsistent results were yielded. This study aimed to understand the association and its gender disparity in Chinese older adults undergoing hospitalisation.

Methods: This multicenter cross-sectional study included 1037 older adults aged 65 years or older.