Observation of Giardia duodenalis infection in Mongolian gerbils: Cysts isolated from bovine feces.

Assemblage E of Giardia duodenalis, primarily infecting ruminants, has been relatively understudied both in vivo and in vitro. Due to unsuccessful attempts at in vitro cultivation, this study focused on establishing an economical, stable, and clinically relevant experimental animal model for Assemblage E infections. Cysts were purified from bovine feces via 33 % zinc sulfate flotation, with Assemblage E identity confirmed by gdh gene sequencing.

Constructing Energetic Ionic Frameworks of Tetrazole with Enhanced Performance through Isomerism.

Ionic framework materials have attracted considerable attention in chemistry and materials science. However, their synthesis predominantly relies on the precise selection of ionic components, often requiring a trial-and-error approach. This limitation has significantly impeded the efficient development of such materials.

Towards an Asian paradigm of inflammatory bowel disease management: A comparative review of China and Japan.

This systematic review compares inflammatory bowel disease (IBD) management between China and Japan across epidemiology, clinical strategies, health insurance, and social security policies. Epidemiologically, the incidence of IBD is rapidly increasing in China, contributing to a growing disease burden. In contrast, Japan has a stabilized incidence but a rising prevalence, driven by an aging patient population.

Metabolite Profiling of Guipi Pill Using UHPLC-Q-Orbitrap-HRMS and Determination of the Pharmacokinetics of its Major Prototype Components.

Guipi Pill (GPP), a classic traditional formula used to treat anxiety disorders, demonstrates significant pharmacological activity. However, research on its pharmacodynamic material basis remains limited. UHPLC-Q-Orbitrap-HRMS was used to systematically analyze the metabolites of GPP in the blood, urine, bile, and feces of rats after oral administration and elucidate its in vivo metabolic processes.

Alginate-encapsulated ZIF-67 composite gel spheres for enhanced tetracycline removal and fixed-bed application.

The pervasive accumulation of tetracycline (TC) in aquatic ecosystems poses severe ecological and health threats, yet conventional technologies rarely achieve reliable removal under continuous-flow conditions. To overcome the intrinsic brittleness, aggregation and leaching of powdered ZIF-67, this study developed novel alginate-encapsulated MOF composite gel spheres (ALG/ZIF-67) via in-situ crystallization. This simple strategy generates hierarchical pores, anchors Co-N active sites and imparts mechanical robustness without sacrificing adsorption kinetics.

Correlation between radiologic feature and spread through air space in lung solid adenocarcinoma 30.0 mm or less in maximum diameter.

Purpose: Tumor spread through air space (STAS) is recognized as an important prognostic indicator for lung cancer patients. However, few studies have focused on radiologic features for predicting STAS in patients with small solid lung adenocarcinomas 30.0 mm or less in maximum diameter.

Effect Evaluation and Mechanism Study of Polyether Surfactant-Assisted Supercritical Carbon Dioxide-Oil Miscibility Based on the Visible Vanish of Interface Measurement Method.

Research on reducing the minimum miscible pressure (MMP) of CO and oil is imperative for the advancement of CO flooding, which can effectively enhance the recovery of low-permeability reservoirs and enable CO geological storage. However, the conventional methods of determining the MMP (e.g.

Research Progress of Platinum-Based Complexes in Lung Cancer Treatment: Mechanisms, Applications, and Challenges.

Platinum-based complexes, as one of the mainstay chemotherapeutic agents in oncology, have evolved from first-generation Cisplatin to third-generation Oxaliplatin, with numerous platinum complexes currently under clinical investigation. This review systematically evaluates all existing platinum complexes for lung cancer treatment, including three generations of approved agents and investigational candidates, with comprehensive analysis of therapeutic efficacy and toxicity profiles. Our assessment aims to provide novel insights for developing next-generation platinum complexes with optimal therapeutic potential.

Retinal proteomic analysis reveals ON/OFF visual stimulation-specific changes in a Guinea pig myopia model.

This study aimed to explore the effect of artificial dynamic ON/OFF stimulation on the development of myopia and associated retinal proteomic changes in guinea pigs, and elucidate the underlying mechanisms. Myopia was induced in one eye of guinea pigs using -4 diopters lenses, with the other eye used as the untreated control. 53 guinea pigs were randomly given balanced ON/OFF (natural control, NC), ON, or OFF stimulation for 7 days.

Mindfulness and anxiety among medicine college students: a moderated mediation model of intolerance of uncertainty and neuroticism.

Anxiety is commonly present in medical students. Mindfulness can effectively treat anxiety, but the mechanisms are poorly understood. This study aimed to investigate the association between mindfulness and anxiety, and the mediation roles of intolerance of uncertainty (IU) and neuroticism.

Dual-Targeting Mn@CeO Nanozyme-Modified Probiotic Hydrogel Microspheres Reshape Gut Homeostasis in Inflammatory Bowel Disease.

Oral microecological agents show potential in reshaping intestinal microbiota and treating inflammatory bowel disease (IBD), but their clinical application is hindered by gastrointestinal challenges, antioxidant instability, and ineffective targeted delivery. In this study, we proposed a protective modification strategy utilizing a nanozyme coating and an alginate microsphere system to enhance the delivery efficiency, effectiveness, and precision of probiotics. By incorporating Mn into CeO, Mn@CeO nanozyme was synthesized, significantly boosting ROS scavenging activity both and at safe dosages.

Post-COVID-19 multimorbidity incidence by prior vaccination status in people with a pre-existing comorbidity: A population-based cohort study.

Background: Long-term health consequences of COVID-19, particularly among individuals with pre-existing chronic diseases, are not fully understood. This study investigates whether SARS-CoV-2 infection increases the risk of developing multimorbidity (≥2 chronic conditions) and evaluates protective effects of vaccination.

Methods: We analyzed territory-wide electronic health records from Hong Kong, linking Hospital Authority data with COVID-19 infection and vaccination records from the Department of Health.

Lessons from the coronavirus disease 2019 pandemic: Investigating the bidirectional relationship between coronavirus disease 2019 and migraine based on the MECH-HK cohort study.

Background: Although the coronavirus disease 2019 (COVID-19) pandemic has ended, its impact on patients with migraine has revealed critical gaps in understanding how migraine interacts with respiratory infections. Exploring this bidirectional relationship is essential for improving migraine management and protecting vulnerable populations during future outbreaks.

Objectives: To examine (1) the impact of migraine on COVID-19 manifestations and the risk of post-acute sequelae of COVID-19 (PASC), and (2) the effect of COVID-19 infection on migraine features.

Ipriflavone ameliorates intervertebral disc degeneration by inhibiting osteoporosis of vertebral body and pyroptosis of the nucleus pulposus in instability of lumbar spine and diabetic mice.

Background: Diabetes mellitus (DM) is a chronic metabolic disease, which can not only induce osteoporosis but also accelerate intervertebral disc degeneration (IVDD). Ipriflavone (IP), as a derivative of isoflavones, can not only control the level of blood glucose, but also improve the regulation of osteoporosis and cartilage extracellular matrix metabolism. However, there is no study on whether IP can effectively improve DM with IVDD.

Fluorescent Tetraphenylethylene-Based Europium Metal-Organic Framework for White-Light-Emitting Diodes.

A luminescent europium-based metal-organic framework (Eu-MOF), designated as , has been synthesized. This MOF employs a tetraphenylethylene derivative ligand that exhibits aggregation-induced emission (AIE) characteristics. Comprehensive structural analyses reveal that crystallizes in the orthorhombic system and boasts remarkable thermal stability.

The across-trial accumulation of cardinal illusion in orientation estimation attenuates the significance of serial dependence.

The human visual system employs two complementary mechanisms-feature contrast enhancement and continuity maintenance-to effectively process extensive visual input. However, how these mechanisms interact to shape visual perception remains poorly understood. In this study, participants were asked to estimate the orientations of a serial of Gabor patches.

Ultra-processed Foods and Diet Quality in Association With Long-term Weight Change and Progression to Type 2 Diabetes Among Individuals With a History of Gestational Diabetes Mellitus-A Prospective Study.

Objective: We examined the associations of overall and group-specific ultra-processed food (UPF) consumption with long-term weight change and type 2 diabetes (T2D) progression following gestational diabetes mellitus (GDM)-complicated pregnancies.

Research Design And Methods: We included 4,207 women with a history of GDM from the Nurses' Health Study II (1991-2021). UPF intake (servings/day) was assessed via food frequency questionnaires every 4 years and quantified per the NOVA classification.

A neonatal rat model of progressive left ventricular pressure overload induced by abdominal aortic banding microsurgery.

Objective: Left ventricular pressure overload models using adult mice or rats were developed 60 years ago; however, a neonatal mouse model of left ventricular pressure overload was reported only 5 years ago. Moreover, how left ventricular pressure overload reshapes the neonatal left ventricle and how it affects cardiomyocyte proliferation remain largely unexplored. The aim of this study is to develop a simple neonatal rat model with clinical features matched to those of left ventricular pressure overload.

Integrated Transcriptome and Metabolome Analyses Uncover Cholesterol-Responsive Gene Networks.

Cholesterol stress profoundly modulates cellular processes, but its underlying mechanisms remain incompletely understood. To investigate cholesterol-responsive networks, we performed integrated transcriptome (RNA-seq) and metabolome (LC-MS) analyses on HeLa cells treated with cholesterol for 6 and 24 h. Through transcriptomic analysis of cholesterol-stressed HeLa cells, we identified stage-specific responses characterized by early-phase stress responses and late-phase immune-metabolic coordination.

Plasma Metabolome, Long-term Body Weight Change, and Type 2 Diabetes in U.S. Individuals.

Objective: Body weight influences the blood metabolome, but whether specific plasma metabolomic profiles predict long-term weight change remains unclear. We aimed to investigate associations among plasma metabolites, current weight, weight change trajectories, and incident type 2 diabetes (T2D) in adults.

Research Design And Methods: We profiled 260 plasma metabolites in 7,499 U.

A case report of hereditary spherocytosis complicated by massive splenomegaly and cholelithiasis.

Here, we report the case of a 53-year-old male patient with hereditary spherocytosis (HS) and a 10-year course of disease, presenting with fatigue, jaundice, abdominal discomfort, massive splenomegaly (spleen size: 35 × 20 × 10 cm, weight: 10 kg), and cholelithiasis. The patient had a positive family history, with his father and daughter exhibiting similar symptoms. Combined splenectomy and cholecystectomy significantly alleviated hemolytic jaundice, anemia, and gallstone-related symptoms.

Bidirectional Relationship Between Dental Diseases and Sleep Disturbances in Pediatric Patients.

Background: Certain dental diseases in pediatric patients may disturb their sleep, affect their oral health-related quality of life, and result in a negative influence on cognition and behavior. On the other hand, sleep disturbances may also increase the risk or participate in development and progression of dental diseases.

Objective: This narrative review aimed to overview of the bidirectional relationship between common dental diseases and sleep disturbances, as well as the potential mechanisms behind.

TC2N promotes the proliferation and invasion of head and neck cancer cells with the p53-R175H.

Background: Tandem C2 domains, nuclear (TC2N) is a recently identified tumor-associated gene that has been confirmed to play important roles in various malignancies, including lung cancer, breast cancer, gastric cancer, glioma, and liver cancer. However, its biological functions and mechanisms in head and neck squamous cell carcinoma (HNSCC) remain unclear. In this study, we aimed to analyze the expression and methylation status of TC2N using public databases and preliminarily investigate the effects of TC2N on the malignant phenotypes of HNSCC cells and its potential mechanisms.

A nine-gene signature with potential targets for predicting the prognosis of patients with esophageal cancer.

Background: The incidence of esophageal squamous cell carcinoma (ESCC) is high and the prognosis is poor. It has become one of the important factors threatening the economic development and social stability of the region. The lack of effective early diagnostic biological indicators is the main reason for the late visit time and high mortality rate of clinical patients with ESCC.