The relationship between occupational stress and burnout among primary and secondary school physical education teachers: evidence from multiverse-style analysis and diary method.

Background: To investigate the relationship between occupational stress and burnout among primary and secondary school physical education (PE) teachers and its underlying mechanisms.

Method: Study 1 utilized a large cross-sectional sample of 4,397 teachers and employed multiverse-style analysis to examine the robustness of the relationship between occupational stress and burnout. Study 2 applied a 21-day diary method to a sample of 324 teachers to explore the intrinsic mechanisms through which occupational stress affects burnout.

Grape Seed Proanthocyanidins Mitigate Acute High-Altitude Hypoxia-Induced Brain Injury by Inhibiting NLRP3 Inflammasome via Autophagy Pathway.

Grape seed proanthocyanidins (GSPs) have been receiving extensive attention due to their outstanding brain protection role in various disease models. This study aimed to explore the therapeutic effects of GSPs against acute high-altitude hypoxia-induced brain injury (AHHBI) and elucidate the underlying mechanism. SD rats and PC12 cells were employed in this study to establish in vivo and in vitro models of acute high-altitude hypoxia, respectively.

Risk factors for complications after infantile enterostomy: development of a clinical prediction model.

Background: Enterostomy is a common surgical procedure for treating acute abdomen in infants. However, the associated complication incidence is high, which significantly impacts infants' recovery. This study aimed to identify risk factors of short-term complications and develop a prediction model in infants with enterostomy.

Mechanism study on the attenuation of cerebral ischemia-reperfusion injury by LBP extract through regulation of SIRT1/PGC-1α axis.

Objective: This study aims to determine if polysaccharides (LBP) extract attenuate oxidative stress by regulating the SIRT1/PGC-1α axis, potentially ameliorating oxygen-glucose deprivation/reperfusion (OGD/R)-induced neuronal damage.

Methods: A cellular hypoxia/reoxygenation model (OGD/R) using HT22 cells was established to simulate cerebral ischemia-reperfusion injury. Cells were allocated into four groups: normal (Control), hypoxia (OGD/R), LBP extract-treated (OGD/R + LBP at 25, 50, 100 μg/mL), and SIRT1-inhibited (OGD/R + S100).

Factors Associated With Physical Activity in Home-Based Rehabilitation Patients With Ankylosing Spondylitis: A Prospective Longitudinal Study.

Background: To identify factors associated with physical activity levels among patients with ankylosing spondylitis (AS) who are undergoing home-based rehabilitation.

Methods: A 6-month follow-up study of 225 AS patients in remission was conducted in a hospital. Data were collected at patient discharge (T0) and at 1 month (T1), 3 months (T2) and 6 months (T3) postdischarge via questionnaires that assessed a range of physiological, psychological, social and cognitive variables.

Development and implementation of a TaqMan triplex real-time PCR assay for concurrent detection of pseudorabies virus, porcine teschovirus 1, and .

Introduction: Porcine neurological disorders represent a prevalent clinical condition that leads to significant mortality and economic losses within the swine industry. Pseudorabies virus (PRV), porcine teschovirus 1 (PTV1), and (SS2) are key viral and bacterial pathogens implicated in the manifestation of neurological symptoms in pig populations. The overlapping clinical presentations and pathological alterations associated with these pathogens pose challenges in their clinical differentiation.

Effects of Paeoniae Radix Rubra on lowering lipid via bioinformatics and gut microbiome.

Hyperlipidemia, a metabolic disorder characterized by abnormal lipid levels, is closely linked to an increased risk of cardiovascular disease. In this study, we investigated the hypolipidemic properties of Paeoniae Radix Rubra and its regulatory effects on gut microbiota composition in a high-fat diet model. Using UHPLC-QE-MS/MS, we identified its chemical constituents and applied bioinformatics, network pharmacology, and molecular docking to virtually screen for bioactive compounds and molecular targets.

Application of Oral Mucosal Epithelial Cells in Noninvasive Pathological Diagnosis of Neuronal Intranuclear Inclusion Disease.

We employed a novel method for collecting oral mucosal epithelial cells, an anagar-paraffin double-embedding technique for cell fixation in cytopathological processing. OMEC biopsy demonstrated a high diagnostic efficacy for NIID: H&E staining revealed inclusions in 60% (12/20) of patients, TEM confirmed them in 40% (8/20) of cases and immunofluorescence detected p62-positive aggregates in 9/20 (45%) and uN2CpolyG in 8/20 (40%) of cases, respectively. OMEC biopsy is a simple, noninvasive technique for detecting intranuclear inclusions in NIID, avoiding the need for invasive surgical procedures such as skin or labial gland biopsies.

Unleashing Electrocatalytic Oxygen Evolution Activity: Engineering Spin States in Strained Correlated Oxides for Enhanced Performance.

Perovskite oxides have emerged as compelling contenders for catalyzing the oxygen evolution reaction (OER) due to their low cost, high efficiency, and structural flexibility. Nevertheless, unraveling the intricate structure-activity relationships within correlated oxides remains challenging, impeding the rational design of efficient catalysts. Here, using LaCoO epitaxial thin films as a model system, we illustrate a direct correlation between the spin state and OER activity.

NAT10 inhibition alleviates astrocyte autophagy by impeding ac4C acetylation of mRNA in ischemic stroke.

Although a single nucleotide polymorphism for -acetyltransferase 10 (NAT10) has been identified in patients with early-onset stroke, the role of NAT10 in ischemic injury and the related underlying mechanisms remains elusive. Here, we provide evidence that NAT10, the only known RNA 4-acetylcytidine (ac4C) modification "writer", is increased in the damaged cortex of patients with acute ischemic stroke and the peri-infarct cortex of mice subjected to photothrombotic (PT) stroke. Pharmacological inhibition of NAT10 with remodelin on Days 3-7 post-stroke or astrocytic depletion of NAT10 targeted virus attenuates ischemia-induced infarction and improves functional recovery in PT mice.

Isolation and possibility of vertical transmission of G9P[23] and G12P[7] group A rotavirus strains in pigs.

Background: Porcine group A rotavirus (RVA) is a significant causative agent of diarrhea in piglets, leading to substantial economic losses in pig farms worldwide. While horizontal transmission of RVA among pig populations is well documented, the possibility of vertical transmission from sows to newborn piglets has not been definitively confirmed.

Results: In this study, piglet testicles, umbilical cord blood, and colostrum were collected from porcine RVA (PoRVA)-active farms for analysis.

Operational parameters regulate in-situ emissions of volatile organic compounds (VOCs) and greenhouse gases (GHGs) during kitchen waste composting: Linking functional microbial dynamics to emission mitigation.

Emission of volatile organic compounds (VOCs) and greenhouse gases (GHGs) from kitchen waste composting plants causes significant environmental issues. This paper characterized the emission profiles of VOCs and GHGs from KW composting with different operating parameters. Quantitative results showed that emission intensities spanned 7.

BHLHE40 contributes to allergic asthma progression in mice through NRTN downregulation in macrophages.

Alternatively activated M2-like macrophages have a profound impact on asthma pathogenesis. Basic Helix-Loop-Helix Family Member E40 (BHLHE40), a dimeric transcriptional factor, plays a key regulation in macrophage functions. Here we show that ovalbumin (OVA)-challenged mice exhibited greater expression of BHLHE40 in lung tissues.

RPA-CRISPR/Cas12a detection based on HCMV-UL123 gene: A way with higher detection rate than clinical detection methods.

Objective: Human cytomegalovirus (HCMV), a prevalent double-stranded DNA enveloped virus, poses a threat to immunocompromised individuals. The current clinical detection methods are insufficient in sensitivity, highlighting the need for more effective approaches.

Methods: We designed and screened RPA primers and crRNA based on the UL123 gene of HCMV.

Integrative transcriptomic and metabolomic analysis reveals the flavor formation mechanism of green bamboo () shoots.

Green bamboo () represents a crucial Asian grass with medical and edible value. However, the unpalatable flavor of emerging bamboo shoots has reduced consumer preference. The distinct flavor of bamboo shoots could potentially be attributed to the accumulation of differential metabolites linked to variations in bamboo shoots maturation, leading to a characteristic taste.

Astrocytic connexin43 in the medial prefrontal cortex regulates depressive- and anxiety-like behaviors via ATP release.

Major depressive disorder (MDD) affects 17 % of the global population and is highly comorbid with anxiety disorders. Emerging evidence indicates that dysregulation of astrocytic ATP contributes to the pathophysiology of depression. However, the molecular substrates underlying the stress-induced reduction in ATP release remain poorly understood, and the basis for the comorbidity of depression and anxiety disorders is still unknown.

Unraveling the role of adjuvant chemotherapy in elderly triple-negative breast cancer: Insights from competing risk analysis using SEER data.

Background: Triple-negative breast cancer (TNBC) is an aggressive subtype with poor outcomes, especially in elderly patients. While chemotherapy is the main systemic treatment, its survival benefits for Patients with TNBC aged ≥ 70 years remain unclear due to comorbidities and treatment intolerance. This study assessed the impact of adjuvant chemotherapy on survival outcomes using competing risk analysis.

Detection Rate of Porcine Circoviruses in Different Ages and Production Herds of Intensive Pig Farms in China.

Porcine circoviruses (PCVs), encompassing porcine circovirus type 1 (PCV1), porcine circovirus type 2 (PCV2), porcine circovirus type 3 (PCV3), and porcine circovirus type 4 (PCV4), have been documented in China and represent a significant threat to the swine industry. Nevertheless, there is a paucity of data regarding the infection characteristics of PCVs across different age groups within intensive pig farming operations. In this investigation, a systematic cross-sectional methodology was employed to collect 415 testicular processing fluid samples and 1583 serum samples from 30 breeding farms and 27 fattening farms in China.

Biochar-loaded iron oxide as a novel electrode for the electro-Fenton degradation of sulfaquinoxaline: Performance evaluation, mechanistic insights, and toxicity transformation.

In this work, carboxymethyl cellulose (CMC)-modified biochar (BC)-supported FeO was prepared for the degradation of sulfaquinoxaline (SQX) in a heterogeneous electro-Fenton process. The degradation rate of 10 mg/L SQX reached 94.2 % after 180 min of FeO(CMC)/BC treatment, compared to 61.

Cross-sectional analysis of posterior reversible encephalopathy syndrome in pregnant women with and without HELLP syndrome.

Aims: This study aims to examine clinical, laboratory, neuroimaging and perinatal differences between pregnant women with isolated PRES and those with concurrent HELLP syndrome, thus improving clinicians' understanding of these conditions.

Methods: A cross-sectional analysis was performed on 60 pregnant and postpartum women diagnosed with PRES at the Affiliated Hospital of Xuzhou Medical University, spanning from January 2014 to March 2024. These patients were divided into two groups based on laboratory findings: the isolated PRES group and the PRES-HELLP group.

Urban-rural difference and scale effect of ecosystem services of blue-green infrastructure.

Blue-green infrastructure (BGI) constitutes the mainstay of urban ecological infrastructure and is a vital element in shaping urban ecological security patterns. Current research on urban BGI primarily focuses on spatio-temporal variations, driving mechanisms, connectivity, and planning management. Less attention has been paid to the spatio-temporal dynamics, coordination, and trade-off mechanisms of BGI ecosystem services (ES) under the background of rapid urbanization.

The relationship between implicit absenteeism and grit and compassion fatigue in female neonatal nurses.

Introduction: Implicit absenteeism is a growing concern among nurses, as it may reduce nursing productivity and affect the quality of nursing services and patients' health. This study aimed to investigate the status and influencing factors of implicit absenteeism among female nurses in neonatal units and to explore the correlations between compassion fatigue, grit, and implicit absenteeism.

Method: An anonymous online questionnaire was administered to female nurses in a neonatal unit from August to October 2024 using the General Information Questionnaire, Stanford Implicit Absenteeism Scale, Compassion Fatigue Short Scale, and self-reported short Grit Scale (Grit-S).