Epidemiological characteristics and environmental contamination of monkeypox in Beijing's Chaoyang district: Field evidence from 2023 to 2024.

Objectives: The mpox outbreak in Beijing's Chaoyang District after late May 2023, this study aims to evaluate the epidemiological characteristics and environmental contamination of this outbreak.

Methods: A field investigation was conducted to evaluate the epidemiological characteristics of mpox cases and their living environments in Chaoyang District, Beijing, between May 2023 and May 2024. Laboratory testing, epidemiological description, and statistical analysis were conducted subsequently.

Heat Shock Protein 70 in Cold-Stressed Farm Animals: Implications for Viral Disease Seasonality.

The seasonal patterns of viral diseases in farm animals present significant challenges to global livestock productivity, with cold stress emerging as a potential modulator of host-pathogen interactions. This review synthesizes current knowledge on the expression dynamics of heat shock protein 70 (HSP70) in farm animals under cold-stress conditions and its potential roles as (1) a viral replication facilitator and (2) an immune response regulator. This review highlights cold-induced HSP70 overexpression in essential organs, as well as its effects on significant virus life cycles, such as porcine epidemic diarrhea virus (PEDV), porcine reproductive and respiratory syndrome virus (PRRSV), and bovine viral diarrhea virus (BVDV), through processes like viral protein chaperoning, replication complex stabilization, and host defense modulation.

Redox-coupled coordination strategy for xanthate detoxification via self-catalysis over a broad pH range.

This study developed an effective strategy to construct a Cu(II)/Cu(I) redox cycle catalytic system by anchoring Cu(II)/Cu(I) onto multi-walled carbon nanotubes (MWCNTs) via wet ball milling for the efficient degradation of xanthate compounds. The catalyst exhibited dual functionality: Cu(II) coordinated with electron-rich S-CS moiety in xanthate molecules, facilitating strong adsorption of xanthate and generation of stable Cu(I), which subsequently activated HO to regenerate Cu(II) and generate reactive oxygen species (•OH, •O⁻, and O). DFT calculations verified the enhanced adsorption of xanthate.

Manipulation of conserved BnFUSCA3s gene instead of using its allelic variation increases the seed oil content of rapeseed.

FUSCA3 (FUS3) is a B3 domain transcription factor that plays a vital role in regulating embryo development. Despite its importance, the exact mechanism by which FUS3 regulates seed oil biosynthesis remains unclear. In our study, we identified four BnFUS3 members in Brassica napus, sharing closely conserved gene structure and protein sequences.

Integrated multi-omics characterization across clinically relevant subgroups of long COVID.

When SARS-CoV-2 became regional epidemics, a substantial number of patients suffered from post-acute sequelae of COVID-19 (PASC, aka long COVID). Exploring the pathogenesis and especially the heterogenicity features of long COVID subgroups is of paramount importance for understanding its etiology. In this study, through integrative multi-omics analyses encompassing transcriptomics, proteomics, and metabolomics, long COVID patients exhibited overall elevated MAPK pathway activation, while patients who have recovered from long COVID showed down-regulation of this response.

Storage of thawed cryoprecipitate for 5 days at refrigerated temperatures: A systematic review and meta-analysis.

This systematic review and meta-analysis aim to evaluate the changes in coagulation factor and bacterial contamination by extending the preservation period of thawed cryoprecipitate for 5 days at refrigerated temperatures. Literature searches were conducted from three different databases, and the literature was screened according to the inclusion criteria. The quality of the literature was assessed using the Risk of Bias in Non-randomised Studies-of Interventions (ROBINS-I) tool, and a meta-analysis was conducted using RevMan5.

Gadolinium-doped red carbon dots exert inhibitory effects on catalase activity: An integrated investigation of computational modeling and cellular responses.

Catalase, a vital antioxidant enzyme, plays a pivotal role in maintaining cellular redox homeostasis. Dysregulation of its activity in tumor cells has emerged as a promising target for cancer therapy. Given the ultra-small size and unique physicochemical properties of carbon dots, which enable effective binding to catalase, this study aimed to investigate the inhibitory mechanism of gadolinium-doped red carbon dots (Gd-CDs) on catalase activity and their resultant biological effects.

Sexual dimorphism in the immune response of Drosophila melanogaster to the entomopathogenic fungus Metarhizium anisopliae.

The immune response of insects to pathogenic micro-organisms infection usually exhibits significant sex differences. Understanding this sexual dimorphism elucidates how immunity interplays with reproductive strategy, metabolic status and environmental factors. In this study, we showed female Drosophila melanogaster (fruit fly) survived better than males when infected with Metarhizium anisopliae CQMa421.

Integrative Analysis of Novel Ferroptosis-Related Genes Signatures as Prognostic Biomarkers in Ovarian Cancer.

Background: Ferroptosis, an iron-dependent form of cell death, has been implicated in the pathogenesis of several types of cancer. Nevertheless, the exact correlation between ferroptosis-related gene mutations and their influence on ovarian cancer (OV) diagnosis and treatment strategies remains to be fully elucidated. It is crucial to identify the ferroptosis-related gene signature in OV and elucidate the impact of these mutations and their expression on the diagnosis and treatment of OV.

The Isoforms of Ral Guanine Nucleotide Dissociation Stimulator (RalGDS) in LLC-PK1 Cells.

This study investigated the isoforms of porcine-origin Ral guanine nucleotide dissociation stimulator (RalGDS) in LLC-PK1 cells using reverse transcription-polymerase chain reaction (RT-PCR) and sequencing. Through segmented amplification, sequence assembly, and comparative genomics analysis, seven RalGDS isoforms were identified, characterized by insertions, deletions, and frameshift mutations. These genetic variations may significantly alter RalGDS's protein structure and function, potentially impacting its role in Ral GTPase-mediated signaling pathways.

Molecular mechanisms underlying the inhibition of cell migration and invasion in endometriosis: Advances in pharmacological research (Review).

Endometriosis (EMS) is a prevalent gynecological disease afflicting reproductive-age women, characterized by the presence of endometrial tissue outside the uterus. The clinical manifestations of this condition include pelvic pain, dyspareunia, and, in severe cases, infertility. The pathology of EMS is similar to that of malignant tumors in terms of implantation, invasion and metastasis, which complicates diagnosis and treatment.

A novel colorimetric immunosensor based on silk cocoon membrane-integrated ELISA for treponemal antibody detection.

The global rise in syphilis incidence and the challenges of early diagnosis highlight the urgent need for convenient diagnostic tools. In this study, we present a colorimetric immunosensor for the detection of antibodies (TP-Ab) using a natural silk cocoon membrane (SCM) as the substrate, based on the principle of enzyme-linked immunosorbent assay (ELISA). The TP monoclonal antibody 3H12 and the chimera antigen TP99, comprising epitopes TP15, TP17, and TP47, were sequentially coupled to functionalized SCM to form the SCM-based TP-Ab immunosensor.

Oncogenic roles of young human de novo genes and their potential as neoantigens in cancer immunotherapy.

Young human de novo genes, recently emerging from non-coding regions, are expected to contribute to human-specific traits and diseases. However, systematic explorations of this connection have been lacking. Here, we report 37 recently originated de novo genes in humans, with their evolution and characteristics defined within an updated genomic context.

Enhancing anti-pilling performance of wool knitted fabrics synergistic treatment with dopamine and silk sericin: a sustainable approach.

Pilling of wool knitted fabrics during wearing or washing has long been a persistent and challenging issue. Conventionally, most anti-pilling treatments are either environmentally unfriendly or cause substantial damage to the internal structure of wool. This study adopts dopamine cross-linking silk sericin to modify wool knitted fabrics.

Lactate and lactylation: emerging roles in autoimmune diseases and metabolic reprogramming.

Autoimmune diseases are a set of conditions in which the immune system incorrectly identifies and attacks the body's own healthy tissue, severely compromising patient health. While current treatments can somewhat control disease progression, their long-term effectiveness remains limited, necessitating the development of more effective therapeutic approaches. Lactate and lactylation are critical links between metabolic reprogramming and epigenetics.

Predicting ESWL success for ureteral stones: a radiomics-based machine learning approach.

Objectives: This study aimed to develop and validate a machine learning (ML) model that integrates radiomics and conventional radiological features to predict the success of single-session extracorporeal shock wave lithotripsy (ESWL) for ureteral stones.

Methods: This retrospective study included 329 patients with ureteral stones who underwent ESWL between October 2022 and June 2024. Patients were randomly divided into a training set (n = 230) and a test set (n = 99) in a 7:3 ratio.

A quantitative analysis of the factors that influence clinical blood demand in the Suzhou area.

Maintaining an adequate blood supply is critical for health care systems, but clinical blood demand is influenced by dynamic, multilevel factors. The aim of this study was to identify and quantify the relationships among the factors that affect clinical blood demand in Suzhou, China, to increase predictive accuracy and supply chain resilience. From March to April 2023, 422 experts in transfusion medicine were surveyed using the Delphi method, and the data were analyzed via exploratory and confirmatory factor analyses (EFA/CFA) to construct a hierarchical structural model.

Exploring the Molecular Mechanisms of Autophagy-related Genes in Hepatic Ischemia/Reperfusion Injury Using Bioinformatics.

Background: Autophagy is a highly conserved cellular process. In the context of hepatic ischemia/reperfusion injury (HIRI), dysregulation of autophagy may lead to hepatocyte dysfunction. Therefore, we conducted a comprehensive transcriptomics analysis to investigate the biomolecular mechanisms underlying autophagy in HIRI.

Scutebarbatine B Exerts Anti-Breast Cancer Activity by Inducing Cell Cycle Arrest and Apoptosis Through Multiple Pathways.

Breast cancer is the most commonly occurring cancer among women with high mortality. Identifying effective anticancer compounds to improve the overall survival is imperative. The present study was designed to evaluate the effects and underlying mechanisms of Scutebarbatine B (SBT-B), a diterpenoid alkaloid extracted from Scutellaria barbata D.

Case report of concurrent ulcerative colitis and bilateral breast cancer: a literature review of the bidirectional association between autoimmune diseases and breast cancer.

A 58-year-old female patient with bilateral breast cancer developed unexpected hematochezia at a frequency of approximately 10 episodes per day following adjuvant chemotherapy, with the emergency endoscopy reporting superficial ulcers throughout the entire colon, suggesting a diagnosis of ulcerative colitis (UC). Given the absence of preexisting autoimmune history or detectable autoantibodies, we supposed that the onset of UC was closely related to the chemotherapy. The complex bidirectional relationship between autoimmune rheumatic diseases and cancer continues to be elucidated.

Early repetitive transcranial magnetic stimulation in the spinal cord region for the treatment of spinal cord injury: A case report.

Rationale: Currently, the global incidence of spinal cord injury (SCI) ranges from approximately 10.4 to 83 cases per million individuals, with an estimated 500,000 new cases diagnosed annually. Current clinical treatments for SCI primarily include early surgical intervention, pharmacological therapy, and personalized rehabilitation programs.

Arthroscopic Management of Massive Irreparable Rotator Cuff Tears: Whole Rotator Cable Reconstruction Using Proximal Biceps Tendon Autograft.

Massive irreparable rotator cuff tears (MIRCTs) are not uncommon in clinical practice, significantly impacting shoulder function and daily activities. Extensive tear size, tendon contracture, and fat infiltration within the rotator cuff pose significant challenges for both patients and clinicians. This type of tear is a key area of interest and a challenge in research and treatments.

Reprocessing status of flexible bronchoscopes in 202 intensive care units in Hubei province, China: a multicentre cross-sectional study.

Background: Unstandardised reprocessing procedures for flexible endoscopes can lead to infection outbreaks and threaten the lives of ICU patients. Despite recent updates to technical specification, there was a paucity of studies on the current status of flexible endoscopic reprocessing, particularly concerning flexible bronchoscopes (FB). This study aimed to assess the current practices of reprocessing FBs in ICUs in Hubei Province, China.