SEE-phAST: Spatially encapsulated emulsions for phenotypic antibiotic susceptibility testing via sequential digital RAA-CRISPR.

The escalating threat of antimicrobial resistance is exacerbated by delayed diagnostics and improper antibiotics use, underscoring an urgent demand for rapid, versatile AST tools to support evidence-based prescribing. In this study, we present an innovative, generalizable phenotypic AST approach by quantifying bacterial gDNA copy number variations (CNVs) following 0.5-h-brief culturing with antibiotic exposure, termed spatially encapsulated emulsions (SEE)-phAST.

Long-term outcomes of vitrectomy for proliferative diabetic retinopathy: a real world study in China.

Purpose: To investigate long-term outcomes and their predictive factors in Chinese patients with proliferative diabetic retinopathy (PDR) after vitrectomy.

Study Design: Retrospective and observational.

Methods: PDR patients who underwent vitrectomy in a tertiary eye hospital from 2010 to 2020 were enrolled.

Near-Infrared-Responsive Digital PCR-Assisted Renal Cancer Exosomal miRNAs Insights and Regulation of Macrophage Polarization.

Exosomes derived from tumors are critical agents in intercellular communication and the tumor microenvironment, offering a rich source of signatures for renal cell carcinoma (RCC) diagnosis. Conventional diagnostic techniques often suffer from limited sensitivity and can be invasive. This study presents an innovative approach using near-infrared (NIR) digital PCR (dPCR) with black phosphorus-embedded gelatin microcarriers for profiling exosomal miRNAs and modulating STAT3 signaling and macrophage polarization.

Contrast-enhanced ultrasound for diagnosing subtypes of intrahepatic cholangiocarcinoma: a comparative study with poorly differentiated hepatocellular carcinoma.

Background: Pathologically, intrahepatic cholangiocarcinoma (ICC) is classified into small-duct (SD) type and large-duct (LD) type, each with distinct clinicopathological characteristics. The contrast-enhanced ultrasound (CEUS) features of the two ICC types remain insufficiently explored.

Purpose: To evaluate liver CEUS imaging for differentiating the SD and LD types of ICC and further compare them with poorly differentiated hepatocellular carcinoma (pHCC).

Class Effect of SGLT2 Inhibitors Against Doxorubicin-Induced Cardiotoxicity via Regulating Adenosine Kinase Mediated-Cardiac Oxidative Stress.

Purpose: Doxorubicin-induced cardiotoxicity (DIC) limits its clinical application. While individual SGLT2 inhibitors have shown cardioprotective effects, it remains unclear whether this is a class effect and whether the underlying mechanisms are shared.

Methods: A mouse model of DIC was established through the administration of six weekly intraperitoneal injections of doxorubicin at a dose of 2.

Integrated multi-omics characterization across clinically relevant subgroups of long COVID.

When SARS-CoV-2 became regional epidemics, a substantial number of patients suffered from post-acute sequelae of COVID-19 (PASC, aka long COVID). Exploring the pathogenesis and especially the heterogenicity features of long COVID subgroups is of paramount importance for understanding its etiology. In this study, through integrative multi-omics analyses encompassing transcriptomics, proteomics, and metabolomics, long COVID patients exhibited overall elevated MAPK pathway activation, while patients who have recovered from long COVID showed down-regulation of this response.

Mild hypothermia regulates neuronal inflammation and oxidative stress through HSP70 to alleviate brachial plexus injury.

Objective: To investigate the function of mild hypothermia (MH) in brachial plexus injury (BPI) by regulating the 70 kDa heat shock protein (HSP70).

Methods: A BPI model mouse was established to investigate the mechanism of MH and HSP70 on BPI through hematoxylin-eosin staining, enzyme-linked immunosorbent assay (ELISA), dichloro-dihydro-fluorescein diacetate (DCFH-DA) staining, and western blotting assays. A cellular model was established by stimulating the motor neuron-like cell line NSC-34 cells with lipopolysaccharide (LPS).

Evaluating the implementation of a longitudinal cocurricular experiential quality improvement training programme for undergraduate medical students.

Quality improvement and patient safety (QIPS) is a core competency in undergraduate medical education. While didactic and experiential learning enhance QIPS knowledge and skills, there are limited experiential opportunities. This study aims to evaluate the feasibility and effectiveness of a longitudinal didactic and experiential student-led programme, Quality Improvement Experiential Student Training (QuEST).

Myeloid MyD88 Mediates Macrophage Infiltration and Activation in Ang II-Induced Cardiac Hypertrophy.

Hypertension is the leading cause of cardiac remodelling and heart failure. Recent evidence has highlighted the role of the non-hemodynamic function of angiotensin II in hypertension. MyD88 is the canonical adaptor of TLRs and IL-1Rs, which serves as the central node in regulating inflammatory responses.

Ultra-Stretchable Helical Fiber Sensor Based on Electromagnetic Induction for Real-Time Human-Machine Interaction.

Fiber-based sensors, owing to their high flexibility, excellent breathability, and ease of integration into everyday clothing, have the potential to serve as a novel type of wearable electronic device. This paper presents a helical strain-sensing fiber using electromagnetic induction, made from Ecoflex and magnetic nanoparticles. It achieves up to 2485% strain and maintains stable voltage output (max 90 μV) over 2000 cycles.

Coral growth records 20 Century sea-level acceleration and climatic variability in the Indian Ocean.

Empirical observations of the rate of sea-level rise (SLR) and the timing of its recent acceleration are critical for validating ensemble methods used to determine global mean sea level trends. Such records are critically important at far-field locations where instrumental datasets are scarce. Here we construct a continuous 90-year sea level record (1930-2019) from the central tropical Indian Ocean derived from the incremental growth of a coral microatoll, which is demonstrated to reflect changes in sea level at annual timescales.

Swt21p Is Required for Nam8p-U1 snRNP Association and Efficient Pre-mRNA Splicing in .

While the U1 small nuclear ribonucleoprotein (snRNP) plays a crucial role in early spliceosome assembly, the mechanisms by which it coordinates with other splicing factors for efficient assembly remain elusive. This study aimed to examine the role of the Swt21 protein in regulating U1 snRNP in . Swt21p was required for efficient pre-mRNA splicing both in vivo and in vitro.

Identification of lipid metabolism-associated biomarkers in lupus nephritis by SVM model and therapeutic potential of Alisol B 23-acetate.

Systemic lupus erythematosus (SLE), a multifaceted autoimmune disorder, has lupus nephritis (LN) as one of its grave complications and is strongly associated with dyslipidemia. This investigation sought to delineate renal-specific lipid metabolism-related genes (LMRGs) signatures in lupus nephritis (LN) through integrated bioinformatics analysis and explore the therapeutic effect of Alisol B 23-acetate (ABA). Analysis of LN datasets revealed multiple dysregulated pathways and identified 11 key LMRGs, including CD36, associated with LN.

The relationship between person-vocation fit and burnout among resident physicians: A single-center cross-sectional survey.

Background: Burnout among resident physicians affects their physical and mental health as well as the quality of healthcare. Person-Vocation Fit (P-V Fit) may play a key role in preventing burnout. This study aims to explore the relationship between P-V Fit and burnout among resident physicians.

A critical need to control thallium contamination in aquatic ecosystem for the age of low carbon.

Thallium (Tl) is a typical toxic metals whose contamination in aquatic ecosystems may increase in the coming decades and become a critical environmental concern. However, our understanding towards the transfer-transformation-mitigation pathways and related mechanism of Tl stemming from different pollution sources remains inadequate, potentially hindering effective pollution prevention, management, and monitoring strategies in the face of escalating Tl contamination in aquatic environments. This review seeks to offer a thorough analysis of the primary sources of Tl pollution, and clarify its environmental impacts in water bodies.

Harnessing PD-1-overexpressing macrophage membrane for preparation of lenvatinib-loaded vesicles to boost immunotherapy against HCC recurrence after radiofrequency ablation.

Hepatocellular carcinoma (HCC) is characterized by high malignancy, high recurrence rate and poor prognosis. Radiofrequency ablation (RFA) is the first-line curative treatment for early-stage HCC. Yet, effective inhibition of local recurrent HCC is still challenging because of immunosuppressive tumor microenvironment (TME) and upregulation of multiple tyrosine kinase receptors in the post-RFA residual tumor.

Asb10 accelerates pathological cardiac remodeling by stabilizing HSP70.

Cardiac hypertrophy is a pivotal risk factor for heart failure. Hypertension-induced pressure overload triggers left ventricular hypertrophy and leads to heart failure. Although the precise mechanisms remain incompletely elucidated, recent studies highlighted the role of ubiquitin-proteasome system in this process.

Changes of diabetic macular edema post vitrectomy in patients with proliferative diabetic retinopathy.

Aim: To investigate the change of diabetic macular edema (DME) post vitrectomy and its risk factors.

Methods: This retrospective study included 365 eyes of 330 patients who underwent vitrectomy for proliferative diabetic retinopathy (PDR) with gradable optical coherence tomography (OCT) imaging from January 2018 to March 2022. The incidence of post vitrectomy DME (PV-DME) was defined as patients with a central retinal thickness (CRT) >300 µm by OCT among patients without preoperative DME.

Potential of CETP inhibition in treating dyslipidemia in systemic lupus erythematosus: Novel and comprehensive evidence from clinical studies and Mendelian randomization.

Objective: Cardiovascular diseases from abnormal lipid metabolism significantly increase mortality in systemic lupus erythematosus (SLE). The causal link between dyslipidemia and SLE is unclear.

Methods: Lipid metabolism in patients with SLE was evaluated based on clinical data from 511 patients with SLE and 706 healthy individuals.

Device closure versus surgical repair for doubly committed subarterial ventricular septal defect.

Objectives: Device closure for doubly committed subarterial ventricular septal defect has been applied in the past decade. However, studies on this issue are largely small-sample, single-arm studies, with inconsistent outcomes. This study aimed to demonstrate that device closure and surgical repair could achieve similar midterm results for patients with doubly committed subarterial ventricular septal defect.

Local Interactions in Aqueous Ethanol Solution Revealed by the C=O Stretching Probe.

Accurately identifying local interactions such as hydrophilicity and hydrophobicity is of critical importance in regulating the functions of amphiphilic biomolecules, but in situ identification methods for such interactions are still lacking. This study proposes a probe based on carbonyl (C=O) stretching vibration to study the hydrophilic and hydrophobic interactions in amphiphilic alcohol-water systems. A combination of theoretical calculations and Raman spectroscopy experiments is employed to investigate the molecular interactions of ethyl acetate C=O in an ethanol aqueous solution, as well as the reasons behind the splitting of spectral peaks.

Explainable machine learning model and nomogram for predicting the efficacy of Traditional Chinese Medicine in treating Long COVID: a retrospective study.

Introduction: Long COVID significantly affects patients' quality of life, yet no standardized treatment has been established. Traditional Chinese Medicine (TCM) presents a promising potential approach with targeted therapeutic strategies. This study aims to develop an explainable machine learning (ML) model and nomogram to identify Long COVID patients who may benefit from TCM, enhancing clinical decision-making.

GLP-1RAs attenuated obesity and reversed leptin resistance partly activating the microbiome-derived inosine/A2A pathway.

Extensive evidence has demonstrated that glucagon-like peptide-1 receptor agonists (GLP-1RAs) can ameliorate obesity. Our previous studies revealed that (Ex-4)-Fc, a long-acting GLP-1RA we developed, depends on the leptin pathway to treat obesity. However, the mechanisms linking (Ex-4)-Fc and leptin resistance remain largely unclear.

Plasma Fetuin-B Levels are Associated with Nervous Symptoms and Conduction Velocity in Patients with Painful DPN.

Aim: To assess the plasma concentrations of the novel hepatokine fetuin-B in individuals with type 2 diabetes with or without diabetic peripheral neuropathy (DPN), and to evaluate the relationship among fetuin-B levels, nervous function, and metabolic parameters.

Methods: A total of 333 participants were recruited and divided into three groups: DPN, painful DPN (pDPN), and non-DPN. Metabolic parameters, peripheral neuropathy-associated indices, and general biochemical parameters were also measured.