Study on the isolation of rhizosphere bacteria and the mechanism of growth promotion in winter wheat in response to drought stress.

Introduction: Wheat is one of the three major cereal crops in the world and is susceptible to the effects of drought stress. Rhizosphere microorganisms can affect plant growth by altering nutrient absorption and resistance to stress. Studying the plant-microbe interaction under drought stress to reveal the impact of soil microorganisms on plant growth in dry land has important scientific significance.

Selection of geographical populations suitable for artificial breeding of the Northeast China Brown Frog (Rana dybowskii).

Amphibians, as a group greatly disturbed by human activities, are at increased risk of extinction. Rana dybowskii is an anuran species with both ecological and economic significance. Due to environmental changes and human overexploitation, it has been classified as Near-Threatened.

Serum Pepsinogen as a Biomarker of Gastrointestinal Stromal Tumors (GIST) in Stomach.

Background: Gastric GISTs (GG) are significant mesenchymal tumors. No biomarker has been identified for GG detection. We first observed mucosal atrophy surrounding GG tumors, leading to the hypothesis that localized atrophy may alter serum pepsinogen (PG) levels.

Risk factors for severe COVID-19 and development of a predictive model.

A clinical case‒control study was conducted to identify risk factors for severe COVID-19 and to develop a predictive risk model to provide a reference for the dynamic assessment of the severity of disease in COVID-19 patients. A total of 410 patients with COVID-19 were included in the study, of whom 132 had severe or critical cases. The clinical data of the patients were collected, and the variables were subsequently screened via LASSO regression analysis and 10-fold cross-validation.

mutations promote an immunosuppressive tumor microenvironment via to confer immune evasion in head and neck cancer.

Immune checkpoint inhibitors (ICIs) have transformed cancer therapy; however, their efficacy in head and neck cancer (HNC) remains limited, with only a minority of patients achieving durable responses. Understanding the molecular mechanisms underlying ICI resistance in HNC is therefore crucial. We conducted an integrative analysis of genomic, transcriptomic, and clinical data from 139 ICI-treated HNC patients (MSKCC cohort) and 502 treatment-naïve HNC cases (TCGA cohort).

Exploring the causal relationship between bipolar disorders and sensory, motor, and behavioral disorders: A bidirectional Mendelian randomization analysis.

The interrelationships between bipolar disorder (BD) and sensory, motor, and behavioral disorders are intricate and not well defined. While observational studies indicate potential associations, causal relationships have not been established. This study applies bidirectional Mendelian randomization (MR) analysis to examine potential causal links between BD and these disorders.

Transcriptomic and microenvironment characteristics of triple-negative breast cancer under three different neoadjuvant treatment regimens.

Background: Adding immunotherapy to chemotherapy can modestly improve the pathological complete response (pCR) rate in triple-negative breast cancer (TNBC), while our previous NeoSAC study demonstrated that combining anti-angiogenic therapy can further enhance pCR. However, research on the mechanisms underlying the efficacy differences and biomarker comparisons across these treatment regimens remains insufficient.

Patients And Methods: Female TNBC patients were consecutively enrolled into three groups: chemotherapy (chemo), chemo-immunotherapy (chemo-ICI), and chemo-immunotherapy-anti-angiogenesis (chemo-ICI-AA, from our NeoSAC study, NCT04722718).

Analysis of methylation features associated with neoadjuvant efficacy in HER2-positive breast cancer.

Background: Response to trastuzumab-based neoadjuvant therapy in human epidermal growth factor receptor type 2 (HER2)-positive breast cancer is affected by multiple features of the tumor. Few studies have investigated epigenetic features in these patients. This study investigates whether changes in deoxyribonucleic acid (DNA) methylation patterns are linked to response to neoadjuvant therapy in HER2-positive breast cancer and aims to identify epigenetic markers of treatment resistance.

Mechanism of self-driven naphthol blue black degradation in Fe(III)-activated peracetic acid system: Dominant role of high-valent iron species.

The iron-activated peracetic acid (PAA) system is a novel green advanced oxidation process (AOP), but conventional Fe(III)-activated PAA systems suffer from low efficiency and require co-activator for iron cycling. This study demonstrated efficient degradation of the organic pollutant Naphthol Blue Black (NBB) in a Fe(III)/PAA system without additional co-activator, achieving 95.7 % removal within 7 min, slightly higher than that observed with Fe(II).

Mild hypothermia regulates neuronal inflammation and oxidative stress through HSP70 to alleviate brachial plexus injury.

Objective: To investigate the function of mild hypothermia (MH) in brachial plexus injury (BPI) by regulating the 70 kDa heat shock protein (HSP70).

Methods: A BPI model mouse was established to investigate the mechanism of MH and HSP70 on BPI through hematoxylin-eosin staining, enzyme-linked immunosorbent assay (ELISA), dichloro-dihydro-fluorescein diacetate (DCFH-DA) staining, and western blotting assays. A cellular model was established by stimulating the motor neuron-like cell line NSC-34 cells with lipopolysaccharide (LPS).

Severity-specific immune landscape of COVID-19 revealed by single-cell sequencing.

The Coronavirus disease 2019 (COVID-19) has rapidly become the worst pandemic since the 1918 influenza pandemic. Studies have shown that severe COVID-19 patients have immune dysfunction. To characterize the dysregulated immune response to SARS-CoV-2 infection, we performed a comprehensive analysis of scRNA-seq and scV(D)J-seq in peripheral blood mononuclear cells from mild, moderate, and severe patients.

sp. nov., a novel species isolated from black soil applied with herbicide atrazine in Northeast China.

The strain tm was isolated from black soil applied with the herbicide atrazine in Northeast China. Phylogenetic analyses based on 16S rRNA gene sequences and phylogenomic analysis revealed that the strain tm is classified to the genus . The DNA G+C content of strain tm was 67.

Biodegradation potential of spp. on polypropylene and polystyrene: enhanced degradation through pretreatment.

Introduction: As extensively utilized synthetic polymers, polypropylene (PP) and polystyrene (PS) have raised significant environmental concerns due to their persistent accumulation in ecosystems.

Methods: To enhance biodegradation efficiency, we implemented a dual pretreatment approach combining thermal activation and fenton's reagent oxidation prior to microbial treatment. Through a systematic 50-day incubation experiment with single-strain cultures of five strains ( B251, B253, 4.

Diagnosing Type 2 Inflammation in COPD: Comparison of Blood and Sputum Eosinophil Assessment in the University of California Los Angeles COPD Phenotyping Study.

Background: COPD phenotyping is an approach for developing tailored therapies. The eosinophilic phenotype is associated with exacerbation risk and response to specific treatments. This study evaluates the relationship between sputum and blood eosinophilia, hypothesizing that sputum eosinophil percentage (SpE%) better reflects disease severity and exacerbation risk than blood eosinophil counts (BEC).

Polarization electric field tuning interface electric field in full-spectral response double S-scheme heterojunction to drive synchronous removal of Cr(VI) and RBR X-3B.

Photocatalytic technology provides a sustainable development strategy for the synchronous removal of azo dyes and mordant leaching of Cr(VI). To mitigate the shielding effect of free carriers on the built-in electric field during the photocatalytic process, a double S-scheme piezo-photocatalyst BiWO/BiOCl/BiS (BW-C-S) with full spectral response that can continuously respond to the ultrasound-guided polarized electric field to adjust the built-in electric field to promote the separation and migration of photogenerated carriers was developed. BW-C-S effectively overcame the barriers of carrier separation and migration by utilizing an ultrasonic-optical coupling field, achieving the rapid synchronous removal of reactive brilliant red X-3B (RBR X-3B) (0.

Liposomal glytrexate formulation: improving antitumour efficacy and minimizing toxicity in breast cancer therapy.

Cancer remains a critical global health challenge with increasing incidence rates. This study reports the first successful encapsulation of glytrexate (GTX), a novel 6-substituted-pyrrolo [2,3-d] pyrimidine compound, into liposomes. This innovative formulation increases the stability, half-life, and bioavailability of GTX while significantly reducing toxicity.

Advancements in research on the anti-metabolic dysfunction-associated steatotic liver disease effects and mechanisms of action of traditional Chinese medicine polysaccharides: A review.

Metabolic dysfunction-associated steatotic liver disease (MASLD) has become the most common chronic liver disease for which there are no approved treatments. Polysaccharides are natural products with a wide range of pharmacological activities. A large number of preclinical studies have confirmed that polysaccharides can intervene in the occurrence and development of MASLD at several interrelated levels, including improvement of glycolipid metabolism, antioxidant, anti-inflammatory, and intestinal flora, showing great potential as novel anti-MASLD drugs.

Single-cell expression profiling of bat wing development.

Bats are the only true-flight mammals, with wings formed by elongated digits and wing membranes. Despite the uniqueness, the cellular and molecular aspects of bat wing development remain largely unknown. Here, we use single-cell transcriptomic sequencing to map ~39,000 cells from the limbs of bats (Rhinolophus sinicus) at developmental stages Carnegie stages (CS) 16, 18, and 20.

Impacts of polyethylene microplastics on the performance and mechanism of di-2-ethylhexyl phthalate (DEHP) degradation by two ecotype earthworms.

Di-(2-ethylhexyl) phthalate (DEHP), a ubiquitous agricultural plasticizer, readily leaches into soils. Earthworm-mediated DEHP biodegradation and microplastics (MPs) co-contamination effects on vermicomposting remain uncharacterized. This study evaluated DEHP biodegradation and metabolic pathways in polyethylene microplastic-added soil under earthworm activity.

Changing Burden, Risk Factors, and Projections of Low Back Pain in Adolescents and Young Adults: Insights from the Global Burden of Disease Study 2021.

Study Design: Ecological study.

Objective: To illustrate the spatiotemporal trends, attributable risk factors, and the projected burden of LBP among adolescents and young adults (AYAs) aged 15-39 years.

Summary Of Background Data: Low back pain (LBP) poses a considerable challenge to individuals and society, and current epidemiological trends in AYAs experiencing LBP remain to be explored.

Integrating biocatalysis with continuous flow: current status, challenges, and future perspectives.

Background: Continuous-flow biocatalysis is gaining attention for the synthesis of highly valuable pharmaceuticals and fine chemicals. The integrated approach combines the high activity and selectivity of biocatalysts with the superior efficiency in mass and heat transfer of continuous-flow systems. Considerable progress has been made in the continuous-flow biocatalysis, but there are only a few reviews on the specific challenges and strategies encountered in continuous-flow reactions involving different biocatalysts.

Predictive value of preoperative frailty combined with Controlling Nutritional Status score for postoperative lung infection in breast cancer patients.

Breast cancer, the most common malignancy in women, often requires modified radical mastectomy, which can lead to complications like postoperative pulmonary infections. These infections, with an incidence of 11.26 to 20.

BHLHA15 promotes cervical cancer cholesterol synthesis and tumor progression.

Research suggested that increased cholesterol synthesis is an effect factor in the malignant progression and unfavorable outcome of cervical cancer (CC). Unfortunately, the molecular mechanisms underlying the increased cholesterol synthesis remain elusive. Here, our comprehensive bioinformatics analysis, along with in vitro and in vivo experimental validations, suggests that basic helix-loop-helix family member a15 (BHLHA15) is a pivotal transcription factor (TF) regulating cholesterol synthesis and malignant progression in CC.

[Research on bimodal emotion recognition algorithm based on multi-branch bidirectional multi-scale time perception].

Emotion can reflect the psychological and physiological health of human beings, and the main expression of human emotion is voice and facial expression. How to extract and effectively integrate the two modes of emotion information is one of the main challenges faced by emotion recognition. In this paper, a multi-branch bidirectional multi-scale time perception model is proposed, which can detect the forward and reverse speech Mel-frequency spectrum coefficients in the time dimension.

Synthesis and Mechanistic Study of a Copolymer Demulsifier for Dehydration of Water-in-Oil Emulsion of Crude Oil.

This study presents the synthesis and characterization of a novel polymeric demulsifier, P(AM-EHMA-VBS-VP), through emulsion polymerization for efficient separation of water-in-crude oil emulsions. The synthesis parameters are systematically optimized using orthogonal array design complemented by single-factor experiments. The demulsification performance is evaluated under simulated field conditions, with particular emphasis on dosage optimization and temperature effects.