Integrative analysis of scRNA-seq and bulk RNA-seq to identify lactylation-related gene signatures in lung ischemia-reperfusion injury after lung transplantation.

Background: Protein lactylation has been implicated in stress-responsive cellular mechanisms, yet its role in lung transplantation-associated ischemia-reperfusion injury (IRI) remains undefined.

Methods: Transcriptomic profiles from GSE145989 were analyzed through differential expression analysis (limma) and weighted gene co-expression network analysis (WGCNA). Integrating the identified genes with lactylation-related signatures uncovered key lactylation-related genes (LRGs) as potential targets.

Stability testing of HPCs and MNCs from apheresis products.

Background: Hematopoietic progenitor cells (HPCs) and mononuclear cells (MNCs) are critical components of cell-based therapies, including bone marrow transplantation and regenerative treatments. Evaluation of the characteristics of these products during collection, storage, and transport is essential for maintaining cell viability and functionality. In this study, we evaluated the functional and molecular stability of samples collected for the evaluation of fresh HPC and MNC products.

Percutaneous nephrolithotomy under total X-ray free technique in horseshoe kidney stones: a 10-year experience from a large stone center.

Objectives: To report outcomes of complete ultrasound-guided percutaneous nephrolithotomy (PCNL) for horseshoe kidney (HSK) stones at a high-volume center and evaluate a novel technique (Needle-perc-assisted endoscopic surgery, NAES) for these patients.

Patients And Methods: We retrospectively reviewed all HSK stone patients who underwent PCNL at our institution over a 10-year period. The NAES technique was utilized during the most recent 4 years.

Effect of pressure biofeedback training combined with Liuzijue Qigong training on respiratory function improvement in post-stroke patients with tracheotomy and decannulation: study protocol for a randomised controlled trial.

Introduction: Stroke causes neurological deficits and respiratory dysfunction, with prolonged bed rest exacerbating secondary pulmonary injury. This study evaluated the efficacy of pressure biofeedback training combined with Liuzijue Qigong (LQG) in improving functional outcomes and respiratory function in patients with tracheostomised stroke.

Methods And Analysis: This will be a parallel, single-centre randomised controlled trial involving 66 patients.

Size-Dependent Restructuring of Supported Platinum Cluster Catalysts.

Supported metal clusters have reactivities that depend on their nuclearity and structure. Herein, we present a strategy for precisely controlling the nuclearity of platinum clusters and demonstrate their size-dependent restructuring behavior and catalytic properties. The clusters are located on isolated CeO nanoislands on high-area SiO, and the isolation facilitates control of the migration of the platinum.

Chromatin-associated circRNA ciCRLF3(2) regulates cell differentiation blockage via activating non-homologous end joining-based DNA repair.

DNA damage response (DDR) is a complicated network that responds to DNA lesions to prevent their accumulation; a defective DDR is one hallmark of cancer. Although targeting DDR pathways has been considered as a therapeutic approach, DDR inhibitors have also been reported ineffective for treating some low mutation burden cancers, such as Mixed-lineage leukemia (MLL)-rearranged (MLL-r) leukemia, a clinically fatal and refractory malignancy. Exploring the roles and mechanisms of DDR pathways in these low mutation burden cancers may help understand the chromatin biology and develop therapeutic strategies.

Esophageal pericardial fistula following radiation therapy after placement of an esophageal stent: A case report and literature review.

Esophageal pericardial fistula is a rare complication of esophageal cancer. This article reports the first documented case of an esophageal pericardial fistula following radiotherapy in the presence of an esophageal stent. A 62-year-old man with advanced esophageal and cardiac cancer underwent radiotherapy after esophageal stent placement and subsequently developed an esophageal pericardial fistula.

Mechanistic insights into the dimethylsulfoniopropionate synthesis enzyme BurB.

Unlabelled: Dimethylsulfoniopropionate (DMSP) is one of the most abundant organosulfur molecules on Earth. It possesses various physiological functions in microorganisms and plays key roles in the global climate regulation. BurB, a SET (Suppressor of variegation, Enhancer of zeste and Trithorax) domain-containing enzyme identified from , initiates DMSP synthesis by methylating methionine (Met) to -methyl-methionine (SMM), with -adenosyl methionine (SAM) as a methyl donor.

Sulfated Fucooligosaccharides Ameliorated Neuroinflammation in D-Galactose-Induced Aging Model Mice via the Gut-Brain Axis.

This study was aimed to reveal the neuroprotective effect of sulfated fucooligosaccharides (FOS) in an aging mouse model induced by d-galactose. The results showed that FOS treatment ameliorated inflammation, improved behavioral decline in memory and cognition, and exerted neuroprotective effects. FOS reduced microglia activation by decreasing the expression of P38 mitogen-activated protein kinase (P38 MAPK), cyclic-AMP response binding protein (CREB), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2).

High Energy Storage Performance under Moderate Electric Field in Fine-Grain BiNaTiO-Based Ceramics.

Ceramic-based dielectric capacitors are one of the core components in high-/pulsed-power devices due to the giant power density and ultrafast discharge time. However, the current high recoverable energy density () is widely obtained under a huge electric breakdown strength ( > 500 kV/cm). Herein, a relaxor ferroelectric ceramic based on BiNaTiO-NaNbO-BaZrO is prepared and exhibits a high of 7.

Prevalence of cardiovascular disease risk factors in ethnic minority adults in plateau areas: a cross-sectional study in Yunnan Province.

Background: The aim of this study was to assess the prevalence and clustering of cardiovascular disease (CVD) risk factors among ethnic minorities in plateau areas and their association with sociodemographic characteristics.

Methods: From September 2023 to January 2024, data were collected among ethnic minorities in plateau areas of Yunnan Province through face-to-face interviews. Ordered logistic regression and restricted cubic splines were used to assess the association between sociodemographic characteristics and the risk of clustering of risk factors.

Cost-utility of endoscopic screening strategies for upper gastrointestinal cancer across China: a modeling study.

Introduction: Endoscopic screening for upper gastrointestinal cancer (UGC) is effective, but it's cost-utility across comprehensive strategies remains unclear. We aimed to assess the cost-utility of various endoscopic screening strategies for UGC within the Chinese health care system.

Methods: This study assessed the cost-utility of 40 endoscopic screening strategies using a Markov model.

Regioselective Synthesis of Multiple Thioether-Bridged Peptides via a One-Pot Reaction.

In this work, the previously reported SeODR strategy was used successfully for the synthesis of bicyclic peptides (). with different rings bearing various thioether linkages were prepared through a one-pot reaction; 15 with satisfactory yields were achieved. Cross-linkers including xylylene dibromide (, 2,6-bis(bromomethyl)pyridine (, 4,4'-bis(bromomethyl)biphenyl (), 1,3-dichloroacetone (, and dichloro--tetrazine ( are all compatible with the SeODR approach used for deprotection of the S-acetamidomethyl (Acm) group in peptides.

Hydrogen-Bond Regulated Water Confinement Strategy Enables High-Temperature-Resistant Hydrogel Film for Flexible Rechargeable Batteries.

The poor high-temperature adaptability of the hydrogel electrolyte film limits the performance of hydrogel-based rechargeable batteries. Especially, the deterioration of electrochemical windows at high temperatures leads to a fast decay of cyclic stability. Herein, a hydrogel film containing 6.

Cooperative Interactions Between Bacillus and Lysobacter Enhance Consortium Stability and Fusarium Wilt Suppression in Cucumber.

The rhizosphere microbiome plays a pivotal role in plant health by mediating interactions between hosts, beneficial microbes, and pathogens. However, the ecological mechanisms underlying microbial consortia that suppress soil-borne diseases remain largely unexplored. In this study, we investigated how the biocontrol bacterium Bacillus velezensis SQR9 influences the assembly of the cucumber rhizosphere bacterial community in the presence of the pathogenic fungus Fusarium oxysporum f.

Generation of donor-derived spermatozoa by cross-subfamily transplantation of purple loach (Leptobotia taeniops) spermatogonia into triploid loach (Misgurnus anguillicaudatus).

Germ cell transplantation plays an important role in the conservation of endangered fish species. As one of the most promising and valuable techniques, there are only few successful cases of cross-subfamily germ cell transplantation in fish. The purple loach Leptobotia taeniops, belonging to the subfamily Botiinae, genus Leptobotia, has been classified as "vulnerable" on the "China Biodiversity Red List.

Biofilms: Characteristics and Control Strategies.

The are a family of bacteria that typically cause respiratory, arthritic, and genitourinary disease in humans. spp. of animal origin are also the causative agents of porcine wheezing disease, chronic respiratory disease and arthritis in chickens and other conditions.

Contrast-enhanced ultrasound for diagnosing subtypes of intrahepatic cholangiocarcinoma: a comparative study with poorly differentiated hepatocellular carcinoma.

Background: Pathologically, intrahepatic cholangiocarcinoma (ICC) is classified into small-duct (SD) type and large-duct (LD) type, each with distinct clinicopathological characteristics. The contrast-enhanced ultrasound (CEUS) features of the two ICC types remain insufficiently explored.

Purpose: To evaluate liver CEUS imaging for differentiating the SD and LD types of ICC and further compare them with poorly differentiated hepatocellular carcinoma (pHCC).

A protective role of SRC-1 against aging associated cognitive decline.

Introduction: Research indicates a strong correlation between obesity and the risk of dementia, both are linked to steroid receptor coactivator-1 (SRC-1), a transcriptional coactivator.

Methods: We used RNA sequencing analysis (RNA-Seq) to investigate the transcriptome of SRC-1-KO mice, and identified S100 calcium-binding protein A6 (S100A6), an AD associated gene, as one target of SRC-1. We tested cognitive behaviors in SRC-1-KO mice and mice with a humanized SRC-1 mutation (SRC-1), and performed promoter luciferase assays on S100A6.

ECM-based molecular subtypes define prognostic, EMT status, and therapeutic diversity in IDH-mutant gliomas.

IDH-mutant gliomas show prognostic heterogeneity despite favorable overall outcomes, necessitating refined molecular classification. While the extracellular matrix (ECM) critically regulates tumor progression, immunity, and EMT, its prognostic significance in IDH-mutant gliomas remains largely unexplored. Here, we employed unsupervised clustering of ECM-related genes across multiple glioma cohorts, identifying two distinct molecular subtypes: ECM1 and ECM2.

Comparison of the diagnostic performance of narrow-band imaging endocytoscopy and staining-based endocytoscopy for colorectal lesions.

Background And Aims: Endocytoscopy (EC) enables real-time histopathological evaluation through two modes: narrow-band imaging endocytoscopy (ECNBI) and staining-based endocytoscopy (EC-SB). However, the comparative diagnostic accuracy between ECNBI and EC-SB, and their performance of endoscopists with varying levels of experience remains unclear.

Methods: This retrospective study analyzed 565 colorectal lesions from 433 patients.

Systematic Study of Preparing Porous CaCO Vaterite Particles for Controlled Drug Release.

Porous CaCO vaterite particles have been widely used as drug carriers for biomedical applications due to their high biocompatibility and low production costs. However, controlling the particle size and porosity of CaCO nanoparticles with the desired crystalline phase is still challenging. In this study, we have systematically investigated the preparation of CaCO nanoparticles under various conditions including precursor types/ratios/concentrations, additive concentrations (ethylene glycol), and temperatures.

Urinary 3-methylhistidine as a potential biomarker for sepsis-associated acute kidney injury: multidimensional metabolomics analysis in mice and human.

Background: Sepsis-associated acute kidney injury (SA-AKI) is strongly associated with increased mortality in critical patients. The early detection of SA-AKI is crucial for clinical intervention. This study aims to integrate multiple metabolomics data related to SA-AKI to identify and validate novel metabolic markers.

Ferroptosis and gastric cancer: from molecular mechanisms to clinical implications.

Gastric cancer, one of the leading causes of cancer-related mortality globally, faces challenges in treatment due to limitations in surgery, chemotherapy resistance, and high recurrence rates. Ferroptosis, an iron-dependent form of cell death, induces cell membrane rupture through dysregulated iron metabolism, lipid peroxidation, and the accumulation of reactive oxygen species (ROS), offering a promising therapeutic avenue for gastric cancer treatment. This article systematically explores the core mechanisms of ferroptosis, including iron overload catalyzing lipid peroxidation via the Fenton reaction, dysregulation of antioxidant systems (such as GPX4 and FSP1), and their associations with gastric cancer cell proliferation, metastasis, and resistance.

Bacterial social interactions in synthetic consortia enhance plant growth.

Plant growth-promoting rhizobacteria (PGPR) represent a sustainable method to improve crop productivity. Synthetic microbial consortia have emerged as a powerful tool for engineering rhizosphere microbiomes. However, designing functionally stable consortia remains challenging due to an insufficient understanding of bacterial social interactions.