Energy burden and asthma prevalence in U.S. cities: An emerging social determinant of health.

Background: Energy burden, defined as the inability to afford residential energy consumption, is a pressing public health issue globally and in the U.S. However, its impact on asthma remains largely unknown.

Exploration of zileuton protective mechanisms against vancomycin-associated nephrotoxicity.

Vancomycin is one of the most commonly used parenteral antibiotics for treating drug-resistant bacterial infections, however, it is hindered by nephrotoxicity. We previously demonstrated that zileuton could delay the onset of vancomycin-associated nephrotoxicity in rats. Here, we sought to understand the mechanism(s) of zileuton renal protection.

In Situ Probing of Polymer Residence on the Nanoparticle Surface by Combining Deep Learning and Single-Molecule Fluorescence Tracking.

This study presents a method for in situ analysis of the adsorption and desorption of polymers on the nanoparticle surface within entangled polymer solutions. This method is based on the principle that when a polymer adsorbs onto the nanoparticle surface, the diffusion coefficient of the polymer becomes equivalent to that of the nanoparticle. Consequently, adsorption events can be identified by properly detecting diffusion-state transitions in trajectories acquired through single-molecule fluorescence tracking experiments.

The safety and feasibility of telerobotic cholecystectomy via a 5G network: a prospective controlled clinical trial.

Background: Since advances in the fifth-generation (5G) communication technology have effectively facilitated the development of robotic telesurgery, the popularity of telerobotic operations has increased. The purpose of this study was to evaluate the safety and feasibility of robotic telecholecystectomy with a 5G wireless network using a new domestic robotic surgical system.

Methods: This study is a prospective controlled clinical trial.

The post-synthetic modification of a Cd-MOF and its fluorescence sensing properties.

In this work, a three-dimensional metal-organic framework, [CdL(HO)] (Cd-MOF) was synthesized by using 2-(6-carboxypyridin-2-yl)terephthalic acid ligand (HL); and its post-synthetic modification composite, Eu@Cd-MOF was obtained by one-pot reaction of Euions with Cd-MOF. The FT-IR, PXRD, XPS, EDS, and fluorescence spectra were employed to verify the successful encapsulation of Euions into Cd-MOF. The luminescence measurements showed that Eu@Cd-MOF had a fluorescence quenching response with minocycline (MC), a widely used drug of broad-spectrum tetracycline antibiotics; and its limit of detection (LOD) for MC was 6.

Application of percutaneous lymphatic contrast-enhanced ultrasound in lymphovenous anastomosis microsurgery.

Objective: This study evaluated percutaneous lymphatic contrast-enhanced ultrasound (CEUS) for preoperative localization in preparation for lymphovenous anastomosis (LVA) microsurgery.

Methods: Fourteen healthy volunteers and 14 patients with lower limb lymphoedema were studied. SonoVue® (Bracco, Milan, Italy) was used to measure lymphatic vessel diameters and depths in the dorsal foot, ankle, and lower leg of the subjects.

Labdane-type diterpenoids from Hypoestes phyllostachya inhibit pancreatic cancer stem cells.

Ten previously undescribed labdane diterpenoids, hypochins A-J (1-10), were isolated from the aerial parts of Hypoestes phyllostachya with white spots. Their structures and absolute configurations were elucidated through comprehensive spectroscopic characterization, including IR, HRMS, NMR, electronic circular dichroism analysis, single-crystal X-ray diffraction, and biosynthetic considerations. Notably, compound 10 represents a rare labdane diterpenoid incorporating 16,15;19,6-dilactone units.

[Expression of Concern] Lysine demethylase 2A promotes the progression of ovarian cancer by regulating the PI3K pathway and reversing epithelial‑mesenchymal transition.

Following the publication of this paper, it was drawn to the Editor's attention by a concerned reader that one of the colony‑formation assay images shown in Fig. 2D for the SKOV3 experiments was strikingly similar to the 'SKOV3/0 µM LY294002' image shown in Fig. 5E, if one of the dishes were to be rotated 90°.

Geographical and temporal variations in availability of national price negotiated novel anticancer drugs: a spatial statistical study based on two cross-sectional datasets in China.

Objective: The National Drug Price Negotiation (NDPN) has significantly reduced the prices and improved the nationwide availability of novel anticancer drugs (NADs) in China. However, geographical disparities in their availability remain concerning. This study aims to assess these spatial variations and temporal changes, and the determinants using geographic information system (GIS) and spatial statistical methods.

Machine Learning-Driven Optimization of Therapeutic Substance Composition for High-Hardness, Fast-Dissolving Microneedles for Androgenetic Alopecia Treatment.

Treating androgenetic alopecia (AGA) with platelet-rich plasma (PRP) holds great promise; however, effective and comfortable delivery remains a challenge. Direct injection causes pain, and PRP-incorporated microneedles (MNs) have low hardness and slow dissolution. To tackle this problem, we propose a machine-learning (ML)-driven strategy, which involves integrating the selection of therapeutic substances, orthogonal experiment designs, ML prediction, and Pareto front identification.

Accuracy and reliability of a three-dimensional superimposition method for maxillary jaws and dentition.

Objective: This study aims to develop a method for three-dimensional (3D) superimposition of the maxillary jaws and dentition using Cone Beam Computed Tomography (CBCT) and intraoral scan data, and to evaluate its accuracy and reliability.

Materials And Methods: This study included 32 adult patients with Angle Class II malocclusion who underwent clear aligner therapy (CAT). The patients were categorized into two groups: Group 1 (TMEM: Third Molar Extraction Management group), including 16 individuals who had undergone maxillary third molar extraction prior to treatment.

A Parent-Metabolite Middle-Out PBPK Model for Genistein and Its Glucuronide Metabolite in Rats: Integrating Liver and Enteric Metabolism with Hepatobiliary and Enteroluminal Transport to Assess Glucuronide Recycling.

: Glucuronide recycling in the gut and liver profoundly affects the systemic and/or local exposure of drugs and their glucuronide metabolites, impacting both clinical efficacy and toxicity. This recycling also alters drug exposure in the colon, making it critical to establish local concentration for drugs targeting colon (e.g.

Overexpression of the Gene from the Desert Pioneer Plant Enhances the Drought Tolerance in .

This research centers on the sand-fixing plant known as , from which the β-glucosidase gene was successfully cloned using the molecular cloning method. encodes a hydrophilic and stable protein made up of 193 amino acids, located in the cell membrane. qRT-PCR analysis indicated that the expression of the is closely linked to the drought stress tolerance of .

Accurate, Scalable Structural Variant Genotyping in Complex Genomes at Population Scales.

Comparisons of complete genome assemblies offer a direct procedure for characterizing all genetic differences among them. However, existing tools are often limited to specific aligners or optimized for specific organisms, narrowing their applicability, particularly for large and repetitive plant genomes. Here, we introduce Structural Variants Genotyping of Assemblies on Population scales (SVGAP), a pipeline for structural variant (SV) discovery, genotyping, and annotation from high-quality genome assemblies at the population level.

Cassane diterpenoids with hepatic gluconeogenesis inhibitory activity from the seeds of Caesalpinia decapetala (Roth) Alston.

A chemical investigation of the seeds of Caesalpinia decapetala (Roth) Alston led to the isolation of fifteen cassane diterpenoids, including ten previously undescribed compounds (1-10) and five known analogues (11-15). The structures of 1-10 were determined using H, C, and 2D NMR, HRESIMS, and IR data. Their absolute configurations were confirmed by comparing experimental electronic circular dichroism data with calculated values, coupled with single-crystal X-ray diffraction analysis.

Factors associated with survival and recurrence of temporal bone squamous cell carcinoma: a single-center clinical study.

Background: Temporal bone squamous cell carcinoma (TBSCC) is a clinically rare, yet highly aggressive, malignant tumor of the temporal bone, characterized by a poor prognosis and a high mortality rate.

Aims: The aim of this study was to assess the survival and recurrence rates of TBSCC patients treated at our institution and to identify factors related to postoperative recurrence and prognosis.

Material And Methods: This retrospective study evaluated 81 histologically confirmed TBSCC cases (2000-2020) with follow-up through August 2022.

High-intensity circuit training improves sleep parameters more than cycling-based high-intensity interval training in sedentary young women: A randomized controlled trial.

Objectives: This randomized controlled trial evaluated the effects of high-intensity circuit training vs. cycling-based high-intensity interval training on actigraphic sleep parameters, subjective sleep quality, and psychological health in sedentary young women.

Methods: Forty-two sedentary women were randomly assigned to high-intensity circuit training (n = 14), high-intensity interval training (n = 14), or control (CON; n = 14).

The Application of Six Sigma to Assess the Analytical Performance of Plasma Proteins and Design a Risk-Based Statistical Quality Control Strategy: A Multicenter Study.

Background: This study applied the six sigma model to evaluate plasma protein testing performance in six laboratories, with customized quality control programs and targeted improvements introduced where necessary.

Methods: Internal quality control (IQC) and external quality assessment (EQA) data for plasma proteins were gathered from six laboratories. Sigma values for each analyte were determined based on the coefficient of variation (CV), bias, and total allowable error (TEa).

The role of ELK4 up-regulation in macrophage polarization and its mechanism in connective tissue disease-associated interstitial lung disease.

Objective: To examine the role of ETS domain containing protein ELK4 in macrophage polarization and pulmonary fibrosis in connective tissue disease-related interstitial lung disease(CTD-ILD).

Methods: This study analyzed gene expression datasets (SSc-ILD, ALI, COVID-19) to identify target genes in CTD-ILD. We performed RT-qPCR, Western blot, and immunohistochemistry to assess macrophage polarization and epithelial-mesenchymal transition (EMT) both in vitro and in a mouse model of pulmonary fibrosis.

Identification and Expression Pattern Analysis of Gene Family in .

Sugars Will Eventually Be Exported Transporters (SWEETs) are involved in plant growth and development, particularly in resistance to adverse environments. () exhibits rhizosheath formation and demonstrates notable salt and drought tolerance. We identified 31 sugar transporter family genes () from the genome in the NCBI database and performed bioinformatics analyses, including gene structure, subcellular localization, conserved sequences, promoter cis-acting elements, phylogenetic relationships, and chromosomal localization.

Predictive value of the serum homocysteine-to-albumin ratio for major adverse cardiovascular events after acute myocardial infarction: a retrospective cohort study.

Background: Acute myocardial infarction (AMI) remains a major global health burden, with major adverse cardiovascular events (MACE) significantly affecting long-term prognosis. Although the Global Registry of Acute Coronary Events (GRACE) score is widely used for risk stratification, its complexity limits its clinical utility, especially in resource-limited settings. The serum homocysteine-to-albumin ratio (HAR) is a novel biomarker that combines oxidative stress and inflammatory processes, both of which play key roles in cardiovascular pathophysiology.

Predicting ESWL success for ureteral stones: a radiomics-based machine learning approach.

Objectives: This study aimed to develop and validate a machine learning (ML) model that integrates radiomics and conventional radiological features to predict the success of single-session extracorporeal shock wave lithotripsy (ESWL) for ureteral stones.

Methods: This retrospective study included 329 patients with ureteral stones who underwent ESWL between October 2022 and June 2024. Patients were randomly divided into a training set (n = 230) and a test set (n = 99) in a 7:3 ratio.