Development and validation of a machine learning model for prediction of cephalic dystocia.

Background: Early detection of cephalic dystocia is challenging, and current clinical assessment tools are limited. Machine learning offers unique advantages, enabling the generation of predictive models using various types of clinical data. Our model aims to integrate objective ultrasound data with psychological and sociological characteristics and obstetric treatment data to predict the individual probability of cephalic dystocia in pregnant women.

Combined metabolome and transcriptome to analyze the regulatory network of key enzymes in the synthesis of senkirkine in Emilia sonchifolia.

Emilia sonchifolia (L.) DC. serves as a well-known folk medicinal and edible plant, yet its toxic component senkirkine exhibits hepatotoxicity.

Pan-cancer methylation analysis of circulating cell-free DNA.

Background: Universal cancer screening based on methylation analysis of circulating cell-free DNA (cfDNA) enables multi-organ cancer detection, thereby reducing all-cause mortality and preventing cancer misdiagnosed by guideline-based cancer-specific screening. This study aims to establish a gene methylation panel for blood-based multi-cancer early detection.

Materials And Methods: Bioinformatics analysis and in-house DNA sequencing of various human cancer cell lines and blood from healthy persons were carried out to identify candidate pan-cancer methylation sites.

Rapid, multiplex, one-pot CRISPR/Dx system for detecting cancer fusion genes.

Targeted therapies directed at fusion genes have proven remarkably effective against cancers. Therefore, the rapid and reliable identification of cancer fusion genes can guide subsequent therapeutic treatment and predict prognosis. By integrating the RT-RPA and CRISPR/Cas12a approaches, we developed a one-pot CRISPR/Dx system for the rapid and multiplex detection of cancer fusion genes.

CRISPR/Dx-based colorimetric and electrochemical detection systems for POCT applications.

To realize point-of-care testing (POCT) for human diseases, CRISPR/Cas technology has been integrated with commercial analytical devices and chromogenic substrates such as personal glucose meter (PGM), pregnancy test strips (PTS), and horseradish peroxidase (HRP). These integrated detection platforms enable portable diagnostics, economical, universal, rapid, and high throughput detection. The change in color and electrochemical signal enables visible signal readout and intuitive results without specialized equipment and skillful operators.

Outdoor light spending time, genetic predisposition and incident Parkinson's disease: the mediating effect of lifestyle and vitamin D.

Background: Previous studies suggest that outdoor sunlight exposure was associated with a lower risk of Parkinson’s disease (PD). However, the interaction of genetic predisposition and the potential role of lifestyle risk factors in mediating this association remains unclear from prospective evidence.

Methods: A cohort study based on the UK Biobank enrolled participants between 2006 and 2010, with the latest follow-up in November 2022.

Exploring the combined toxic effects of tri-n-butyl phosphate and polystyrene micro/nano-plastics on Daphnia magna under environmentally relevant concentrations.

As emerging pollutants prevalent in environments and biota, tri-n-butyl phosphate (TnBP) and microplastics (MPs) are harmful to aquatic organisms. Nevertheless, the combined toxicity of TnBP and MPs to aquatic organisms at environmental concentrations is still unknown. In this study, the co-toxic effects of both TnBP and micro/nano-polystyrene (MNPS) in Daphnia magna (D.

Elucidating the Therapeutic Mechanisms of Dendrobium loddigesii in Gastric Mucosal Injury: An Integrated Approach Combining Serum Metabolomics and Network Pharmacology.

Dendrobium loddigesii Hook is a traditional Chinese herbal medicine widely used in China. Modern pharmacological studies have shown that Dendrobium has immune regulation, anti-tumor, anti-oxidation, and anti-aging, hypoglycemic effect and is often used in the treatment of chronic gastritis. However, as an important source of Dendrobium, D.

Branched-chain amino acid and cancer: metabolism, immune microenvironment and therapeutic targets.

Cancer is one of the major diseases threatening human health in the world. According to the latest global cancer statistics from the International Agency for Research on Cancer (IARC), there were approximately 20 million new cancer cases and 10 million cancer deaths worldwide. Amidst this global health concern, branched chain amino acids have emerged as key players, playing an important role in the occurrence and development of cancer.

The Prevalence of Pretreatment Drug Resistance and Transmission Networks Among Newly Diagnosed HIV-1-Infected Individuals in Nanning, Guangxi, China.

The scale-up of antiretroviral therapy (ART) has markedly increased pretreatment drug resistance (PDR) among newly diagnosed HIV-infected individuals. This study aims to assess the prevalence and characteristics of PDR, infer the genetic transmission network, and evaluate the effect of PDR on ART in Nanning City, Guangxi. Methods: This study was conducted in the Fourth People's Hospital of Nanning from 2019 to 2023.

Phillyrin counters β2 integrin-mediated neutrophil adhesion and chemotaxis to alleviate endotoxin-induced acute lung injury in neonatal rats.

Acute lung injury (ALI) in neonates presents a grave threat to infant health, characterized by a heightened risk of mortality. Phillyrin, an extraordinary constituent derived from a traditional Chinese medicinal herb Forsythia suspensa, has garnered considerable attention for its pronounced anti-inflammatory properties. However, its therapeutic potential for acute inflammatory diseases in neonates remains unclear.

Two doses of Qβ virus like particle vaccines elicit protective antibodies against heroin and fentanyl.

Opioid overdoses and opioid use disorder (OUD) are major public health concerns. Current treatment approaches for OUD have failed to slow the growth of the opioid crisis. Opioid vaccines have shown pre-clinical success in targeting multiple different opioid drugs.

Artificial base mismatches-mediated PCR (ABM-PCR) for detecting clinically relevant single-base mutations.

Objectives: Detecting point mutations with high sensitivity and specificity can be technically very challenging, but it is crucial for early diagnosis and effective drug treatment of cancers. To enable ultrasensitive and ultraspecific detection of single-base mutations in simple and economical ways, we have developed an artificial base mismatches-mediated PCR (ABM-PCR) detection approach.

Methods: ABM-PCR was applied to quantitative PCR (qPCR) and droplet digital PCR (ddPCR) detection platforms.

Near-natural conversion of plantations primarily enhances the abundance and functionality of nematodes within medium-sized soil aggregates.

Adopting "close-to-nature" forestry practices to rejuvenate struggling tree plantations has shown promise in restoring soil fauna. However, the micro-scale mechanisms behind these restoration efforts and their impact on soil fauna functions remain poorly understood. We replanted coniferous (Pinus massoniana) plantations with native broadleaf trees (Cinnamomum longipaniculatum) and studied the effects on soil nematodes within soil aggregates after eight years.

USP28-mediated deubiquitination of FOXK1 activates the Hippo signaling pathway to regulate cell proliferation and radiosensitivity in lung cancer.

Aims: Radioresistance remains a significant challenge for lung cancer therapeutics. Forkhead box K1 (FOXK1) plays a role in regulating various biological processes and the progression of multiple cancers. However, the role of FOXK1 in lung cancer progression and radioresistance are not fully understood.

Prevalence of pulmonary dysfunction and its risk factors in patients with transfusion-dependent thalassaemia: protocol for a systematic review and meta-analysis.

Introduction: Pulmonary complications frequently occur as comorbidities in individuals with transfusion-dependent thalassaemia (TDT). Despite their clinical significance, the existing literature lacks a systematic review and meta-analysis examining the prevalence and risk factors for pulmonary dysfunction in this patient cohort. Therefore, we propose to conduct a systematic review and meta-analysis to report the prevalence and associated risk factors of pulmonary dysfunction in TDT patients.

RSK2-mediated phosphorylation and degradation of UBE2O inhibits hepatocellular carcinoma growth and resistance to radiotherapy.

Radioresistance poses the main challenge in radiation therapy (RT) for liver cancer, with the DNA Damage response (DDR) being a crucial component of this resistance. Ubiquitin-conjugating enzyme E2O (UBE2O) has been implicated in regulating tumor proliferation, cholesterol metabolism, and drug resistance. However, the role of the ubiquitin-conjugating enzyme E2O (UBE2O) in DDR of liver cancer remains to be fully explored.

Transcriptome analysis unveils the mechanisms of oxidative stress, immunotoxicity and neurotoxicity induced by benzotriazole UV stabilizer-328 in zebrafish embryos.

As an emerging pollutant, ultraviolet stabilizer-328 (UV-328) has been frequently detected in aquatic environments and attracted great attention. Nevertheless, the toxicity and mechanisms of UV-328 to aquatic organisms are still not fully understood. In particular, the immunotoxicity and neurotoxicity of UV-328 to aquatic organisms and their mechanisms have not been reported yet.

One-pot isothermal CRISPR/Dx system for specific and sensitive detection of microRNA.

MicroRNA (miRNA) is a promising biomarker for the early diagnosis of pancreatic cancer. To enable sensitive and reliable miRNA detection, we have developed a one-pot isothermal CRISPR/Dx detection system by combining rolling circle amplification (RCA) and CRISPR/Cas12a. RCA and CRISPR/Cas12a reactions are carried out in a single closed tube, bypassing the transferring step.

Associations between plasma caspase-1 levels and cardiovascular disease, with the mediating role of metabolic syndrome.

Aims: This study aimed to explore the association between plasma caspase-1 levels and cardiovascular disease (CVD), as well as the potential mediating role of metabolic syndrome (Mets) in the association.

Methods: This study analyzed the UK Biobank Precision Proteomics Project (UKB-PPP), which detected plasma caspase-1 levels in participants. CVD was defined by ICD-9/ICD-10 codes.

Lycorine ameliorates liver steatosis, oxidative stress, ferroptosis and intestinal homeostasis imbalance in MASLD mice.

Background: Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common liver disease worldwide and few drugs are available for its treatment. Lycorine has effective anti-inflammatory and lipid-lowering effects, but the impact on MASLD is not fully understood. In this study, we intend to test the intervention effect of lycorine on MASLD.

Causal relationship between major depressive disorder, anxiety disorder and constipation: a two-sample Mendelian randomization study.

Background: Epidemiological and other studies have shown correlations among major depressive disorder (MDD), anxiety disorder (AXD) and constipation. However, no consensus has been reached regarding their interdependence and pathogenesis. Herein, we sought to further explore the causal associations between them.

SynNotch-Programmed Macrophages for Cancerous Cell Detection and Sensing.

Synthetic Notch (synNotch) receptors have enabled mammalian cells to sense extracellular ligands and respond by activating user-prescribed transcriptional programs. Based on the synNotch system, we describe a cell-based in vivo sensor for cancerous cell detection. We attempted to engineer synNotch-programmed macrophages to sense cancer cells via urinary analysis of human chorionic gonadotropin (HCGB5).

Serum ferritin is a risk factor for pulmonary dysfunction in young patients with transfusion-dependent thalassemia.

Research into the pulmonary function of patients with transfusion-dependent thalassemia (TDT) is limited, with existing studies presenting conflicting results. We carried out a retrospective study involving 140 patients with TDT. The mean patient age was 8.

The Dynamics of Allelochemicals and Phytotoxicity in during the Decomposition of Litter.

Allelopathy is an underlying and controversial mechanism for detrimental environmental effects in the management of plantations. However, little attention has been paid to the dynamics of allelochemicals and phytotoxicity in soil fauna during litter decomposition. To explore the relationship between the dynamics of phytotoxicity and allelochemicals, a decomposition experiment was conducted using 4-year-old and 8-year-old litter (0, 10, 20, 30, and 45 days).