Ocular Surface Alteration on Corneal Endothelial Decompensation and Descemet's Membrane Endothelial Keratoplasty.

Objective: To explore alterations in tears and ocular surface throughout Descemet's Membrane Endothelial Keratoplasty (DMEK), thereby offering valuable insights for clinical intervention.

Methods: This study was conducted on 84 patients (84 eyes) with CED and received DMEK who visited the Zhongshan Ophthalmic Center of Sun Yat-sen University from April 2022 to January 2024. All patients were evaluated preoperatively and 1, 3, 6, and 12 months postoperatively, with 31 patients (31 eyes) post-24 months above.

A nut-and-bolt assembly of the bimodular large progenitor botulinum neurotoxin complex.

Botulinum neurotoxin serotype A (BoNT/A) is naturally produced by bacteria along with four nontoxic neurotoxin-associated proteins (NTNH, HA70, HA33, and HA17), forming a bimodular large progenitor toxin complex (L-PTC). The BoNT/A-NTNH complex protects the toxin from adverse environment, while the complex consisting of HA proteins facilitates toxin absorption during oral intoxication. How these two independent modules assemble into the L-PTC remains unclear.

Characterization of Muscle Tissue Cell Diversity and Clinical Implications in Idiopathic Inflammatory Myopathy.

Background: Idiopathic inflammatory myopathies (IIMs) exhibit diverse cellular microenvironments in muscle tissues, yet the full spectrum of cell populations and changes remains unclear. This study aimed to characterize cellular heterogeneity, explore cell-cell interactions and assess the prognostic value of cell subtype abundances across IIM subtypes in Han Chinese.

Methods: Muscle samples from six IIMs and three normal controls (NC) underwent single-cell RNA sequencing (scRNA-seq), whereas bulk RNA sequencing was performed on 203 IIMs and 19 NC.

Bioinformatic Analysis of the Value of Mitophagy and Immune Responses in Corneal Endothelial Dysfunction.

This study was conducted to elucidate the mitophagy-related differentially expressed genes (MRDEGs) in corneal endothelial dysfunction (CED) and to identify key hub genes that could provide insights into the disease pathogenesis and potential targeted therapies. To achieve this, CED models were established in female SD rats, and RNA sequencing of coronal endothelium samples was conducted to generate a self-testing dataset. Comprehensive bioinformatics analyses were executed, which included the identification of differentially expressed genes (DEGs), GO and KEGG enrichment analyses, GSEA, and GSVA.

Wastewater-based epidemiology of influenza A virus in Shenzhen: baseline values and implications for multi-pathogen surveillance.

Influenza A virus (IAV) has become increasingly unpredictable in its seasonal patterns following the COVID-19 pandemic. A key challenge remains identifying optimal moments for public health interventions to inform evidence-based decisions. Real-time PCR was employed to quantify IAV concentrations in wastewater samples from 38 treatment plants in Shenzhen, China (n = 2,764), collected weekly from March 2023 to March 2024.

The current situation and factors influencing the use of traditional Chinese medicine therapies among patients with chronic disease in china: a cross-sectional study.

Chronic noncommunicable diseases, which can be referred to as chronic diseases, have become a significant challenge to global public health. Various studies have shown that traditional Chinese medicine (TCM) therapies are effective means of preventing and treating chronic diseases. The purpose of our study was to analyze the present circumstances of the use of TCM therapies among Chinese patients who have chronic diseases and explore the related influencing factors.

Proteomic subtypes enrich current acute myeloid leukemia nomenclature and reflect intrinsic pathogenesis alongside aging.

Acute myeloid leukemia (AML) is a highly heterogeneous hematological malignancy that increasingly affects the elderly population, with its post-transcriptional landscape remaining largely elusive. Establishing a stable proteomics-based classification system and systematically screening age-related proteins and regulatory networks are crucial for understanding the pathogenesis and outcomes of AML. In this study, we leveraged a multi-omics cohort of 374 newly diagnosed AML patients, integrating proteome, phosphoproteome, genome, transcriptome, and drug screening data.

A novel prognostic risk score associated with resistance to docetaxel chemotherapy for predicting biochemical recurrence-free survival in patients with prostate cancer.

Objective: To identify molecular clusters and develop a novel prognostic risk score associated with resistance to docetaxel chemotherapy for predicting biochemical recurrence free survival (BCRFS) and tumor immune microenvironment of prostate cancer (PCa).

Materials And Methods: We obtained the clinical and transcriptome data of PCa from TCGA and GEO database. Univariate and multivariate Cox regression analysis were used to establish a novel prognostic risk score associated with resistance to docetaxel chemotherapy for predicting BCRFS.

Mechanism of CaCO3 efficiently promoting hydrogen production by Clostridium butyricum BJ-10.

Aims: This study aims to explore the effect of adding CaCO3 on the hydrogen production by the fermentation of Clostridium butyricum (C. butyricum) BJ-10 and the mechanism of promoting hydrogen production by C. butyricum.

Matrix-assisted Laser Desorption Ionization Mass Spectrometry for Detection of α/β-Chain Changes From Human Hemoglobin: An Exploratory Study.

In this work, we developed a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) method to directly analyze human whole blood samples. 1.0 µL of whole blood samples and 1.

A computed tomography-based deep learning model for non-invasively predicting World Health Organization (WHO)/International Society of Urological Pathology (ISUP) pathological grades of clear cell renal cell carcinoma (ccRCC): a multicenter cohort study.

Background: Clear cell renal cell carcinoma (ccRCC) is the most common and aggressive subtype of kidney cancer, commonly exhibiting significant morphological heterogeneity in its pathological characteristics. The objective of this study is to develop a deep learning (DL) model for predicting pathological grades of ccRCC based on contrast-enhanced computed tomography (CECT).

Methods: Retrospective data were collected from 483 ccRCC patients across three medical centers.

Genetic analysis the causal associations between metal elements and thyroid cancer: a bidirectional Mendelian randomization.

Background: Thyroid cancer is the most common endocrine malignancy worldwide, and recent studies suggest that environmental metal exposure may influence its development. However, existing evidence is mostly observational and lacks causal inference.

Methods: We performed a two-sample Mendelian randomization (MR) analysis to assess the causal relationships between 21 metal elements and thyroid cancer risk in East Asian populations.

Establishment of the early gut microbiota in vaginally delivered infants: the influence of maternal gut microbiota outweighs vaginal microbiota.

Unlabelled: Gut microbiota is one of the largest microbial communities within the human body, yet its origins and early developmental processes remain not fully understood. This study collected samples from 26 mother-infant pairs from the third trimester to 14 days postpartum, using 16S rDNA sequencing and Source Tracker analysis to investigate the dynamic changes and sources of early infant gut microbiota. Results showed that maternal gut microbiota at 32 weeks of gestation was the primary source of meconium microbiota in vaginally delivered infants and continued to influence day-14 gut microbiota, while maternal vaginal microbiota contributed minimally (<1%).

Transparent Degradable Networks from Catalyst-Free Acetal Vitrimers from Peach Gum Polysaccharide for Sustainable Fruit Preservation.

Degradable and recyclable biobased materials offer a sustainable paradigm shift from petrochemical plastics. However, their industrial viability often depends on developing a simple, material-efficient synthetic approach. Here, we report a series of peach gum polysaccharide (PGP)-based vitrimers containing dynamic acetal bonds.

The Value of Lp-PLA2 as a Biomarker for the Diagnosis of Plaque Stability in Atherosclerosis: A Meta-Analysis.

Lipoprotein-associated phospholipase A2 (Lp-PLA2), secreted by inflammatory cells within atherosclerosis lesions, has emerged as a promising biomarker linked to plaque stability. This meta-analysis aims to synthesize current evidence on the diagnostic value of Lp-PLA2 as a biomarker of atherosclerotic plaque stability. Literature were searched from PubMed, Cochrane, Embase, ScienceDirect, SinoMed, Wanfang, and CNKI databases.

Usefulness of Blood-Urea-Nitrogen to Serum Albumin Ratio for In-hospital Mortality Predictions in Atrial Fibrillation Patients Admitted to the Intensive Care Unit: A Retrospective Analysis From MIMIC-IV Database.

Background: Despite prior research showing that elevated BAR levels were linked to poor prognoses in several cardiovascular disease conditions, the predictive role of the blood-urea-nitrogen to serum albumin ratio (BAR) in atrial fibrillation (AF) patients admitted to the intensive care unit (ICU) remains largely unknown.

Methods: Patients diagnosed with AF were gathered from the Medical Information Mart for Intensive Care-IV (MIMIC-IV) database, and the X-tile software was used to determine the best cut-off values for BAR. The Kaplan-Meier curves and receiver operating characteristics (ROC) analyses were used to evaluate the prognostic value of the BAR.

METTL5 regulates SEPHS2-mediated selenoprotein synthesis to promote multiple myeloma survival and progression.

The abnormal expression of mA methyltransferase is a significant factor in the occurrence and progression of tumors. The 18S ribosomal RNA (rRNA) mA methyltransferase Methyltransferase-like 5 (METTL5) is upregulated in various cancers, leading to adverse prognosis by abnormally regulating protein translation in tumor cells. However, the functionality and molecular mechanisms of METTL5 in the progression of multiple myeloma (MM) remain unknown.

Complete mitochondrial genome assembly and comparative analysis of Fagopyrum dibotrys (Golden Buckwheat).

Background: Mitochondria and chloroplasts are crucial plant organelles that harbor valuable genetic information. Fagopyrum dibotrys, commonly known as golden buckwheat, is an economically significant plant with diverse pharmacological properties, including antioxidant, antitumor, and anti-inflammatory activities. However, the mitochondrial genome (mt genome) for golden buckwheat has not yet been sequenced, limiting our understanding of its evolutionary characteristics.

Association of magnetic resonance imaging glymphatic function with gray matter volume loss and cognitive impairment in Alzheimer's disease: a diffusion tensor image analysis along the perivascular space (DTI-ALPS) study.

Background: Diffusion tensor image analysis along the perivascular space (DTI-ALPS) has been used for diagnosing Alzheimer's disease (AD); however, few studies have examined the relationship between the DTI-ALPS index and cortical metrics, and the differentiation between AD severity levels remains unclear. This study aimed to explore the differences in DTI-ALPS index and cortex among AD patients with varying severities and to analyze the interactions between DTI-ALPS index, cortical metrics, and cognitive function.

Methods: A total of 19 individuals with mild cognitive impairment (MCI), 17 individuals exhibiting mild AD, 25 individuals with moderate AD, and 28 healthy controls (HC) who were matched for age, sex, and education level were recruited.

UCP2 inhibition eliminates pancreatic β cell autoinflammation in T2DM with islet-mitochondrial sequential targeting nanomedicines.

Pancreatic β-cell dysfunction and mass loss are core pathologies of type 2 diabetes mellitus (T2DM), which are closely related to intense autoinflammation. However, the molecular mechanisms regulating β-cell autoinflammation remain unclear. Here, we show that STING is significantly elevated in T2DM β cells.

Complement C5aR blockade attenuates LPS-induced acute kidney injury by regulating ferroptosis via nuclear factor-erythroid 2-related factor 2 signaling in mice.

Acute kidney injury (AKI) is a prevalent and severe condition characterized by sudden renal dysfunction. In this investigation, we explored the therapeutic potential of C5a receptor (C5aR) blockade in a murine model of lipopolysaccharide (LPS)-induced AKI. Our findings reveal that LPS administration disrupts renal morphology, while treatment with the C5aR antagonist W54011 effectively reverses these changes.

Efficacy and safety of cross-linked acellular porcine corneal stroma for infectious corneal disease in China: a multicenter prospective clinical trial.

Background: Acellular porcine corneal stroma (APCS) is a promising alternative to allografts, however its potential requires verification. To evaluate the efficacy and safety of novel cross-linked APCS transplantation for the treatment of infectious corneal disease.

Methods: This prospective, multicenter, single-arm clinical trial was conducted from 2015-2019.

Identification and Validation of a Prognostic Model Based on Tumour Necrosis Factor-Related mRNAs for Kidney Renal Clear Cell Carcinoma.

Tumour necrosis factor (TNF) plays a critical role in tumour progression, but the specific involvement of mRNA in this process, particularly in kidney renal clear cell carcinoma (KIRC) remains insufficiently understood. Our study aims to develop a TNF-related mRNA (TRmRNA) model to predict prognosis and inform treatment strategies in KIRC. KIRC expression data from The Cancer Genome Atlas (TCGA) and TNF-related genes (TRGs) from the Genecards database were used to construct and validate a TRmRNA prognostic model.

Analysis of factors influencing tumor volume doubling time in hepatocellular carcinoma and its predictive value for progression-free survival.

This study aims to identify and analyze the factors that affect tumor volume doubling time (TVDT) in hepatocellular carcinoma (HCC) and evaluate how these factors impact progression-free survival. We performed a retrospective study on 183 confirmed cases of HCC at the General Hospital of Ningxia Medical University from January 1, 2018, to December 31, 2023. We utilized 3D Slicer 5.

GSH promote renal cadmium efflux through ABCC1 of ABC family.

Accumulation of cadmium in the kidneys can lead to toxicity; therefore, it is crucial to identify methods that promote cadmium excretion to mitigate nephrotoxicity. However, the mechanisms underlying cadmium excretion from the kidneys remain unclear. The role of the ATP-binding cassette (ABC) transporter family in the renal excretion of cadmium was investigated in this study.