Lactic acid bacteria and yeast co-culture system: Mechanism of quorum-sensing regulation on the high yield of exopolysaccharides in Weissella confusa.

The low yield of exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) restricts their industrial application. To overcome this limitation, a single-factor optimization strategy was applied to develop co-culture system involving Weissella confusa P2 and Candida shehatae. This approach resulted in 48.

Noncollinear harmonic spectroscopy reveals crossover of strong-field effects.

The ability to control electron motion with light fields represents a transformative frontier in modern physics, enabling dynamic manipulation of material properties at ultrafast timescales. Yet, the complex interplay between light and excited carriers-via mechanisms such as the AC Stark effect, field-induced coupling of excitonic and Bloch states, the dynamical Franz-Keldysh effect, and the ponderomotive effect-continues to challenge our understanding of quantum systems driven far from equilibrium. Here, we establish non-collinear harmonic spectroscopy as a powerful technique for initiating, tracking, and steering femtosecond carrier dynamics across the energy landscape in the dielectric SiO crystal.

Genetic mapping of lifespan and mitochondrialstress response in C. elegans.

The mitochondrial unfolded protein response (UPRmt) is one of the mito-nuclear regulatory circuits that restores mitochondrial function upon stress conditions, promoting metabolic health and longevity. However, the complex gene interactions that govern this pathway and its role in aging and healthspan remain to be fully elucidated. Here, we activated the UPRmt using doxycycline (Dox) in a genetically diverse C.

Serum uric acid levels and the risk of hemorrhagic stroke: Insights from a two-sample Mendelian randomization study.

Background And Aims: Observational studies have indicated a potential association between serum uric acid levels and the risk of intracerebral hemorrhage and subarachnoid hemorrhage. To explore whether this relationship is causal, the authors performed a two-sample Mendelian randomization analysis.

Methods And Results: The authors sourced data on single-nucleotide polymorphisms related to serum uric acid levels from the European Bioinformatics Institute ‒ Association database, along with information on intracerebral hemorrhage and subarachnoid hemorrhage from the same repository.

Research progress on genes and regulatory mechanisms of drought resistance in rice.

Global warming and uneven distribution of rainfall have led to frequent drought events. Drought is a major obstacle affecting rice production; therefore, it is crucial to elucidate the drought regulatory mechanisms and breed drought-resistant and water-saving rice varieties. With the rapid development of molecular biology tools, numerous drought-related genes have been identified and cloned.

Research progress of epidermal growth factor receptor in metabolic dysfunction-associated steatotic liver disease and related diseases.

Epidermal growth factor receptor (EGFR), a key receptor tyrosine kinase, regulates cell proliferation, differentiation, metabolism and inflammation. It is implicated in the progression of MASLD, steatohepatitis, liver fibrosis, hepatocellular carcinoma, type 2 diabetes and other metabolic diseases. This review summarised the structure and function of EGFR, its molecular mechanisms in different pathological stages of MASLD (steatosis, inflammation, fibrosis), its association with hepatocellular carcinoma and type 2 diabetes, cardiovascular disease and chronic kidney disease, as well as the research progress of EGFR-targeted therapy and the challenges faced in clinical application.

Nonlinear multi-head cross-attention network and programmable gradient information for gaze estimation.

Gaze estimation is an important indicator of human behavior that can be used for human assistance. Recent gaze estimation methods are primarily based on convolutional neural networks (CNNs) or attention Transformers. However, CNNs extract a limited local context while losing important global information, whereas attention mechanisms exhibit low utilization of multiscale hybrid features.

Dual regulation of FZD1/7 by IGF2BP3 enhances stem-like properties and carboplatin resistance in triple-negative breast cancer.

Triple-negative breast cancer (TNBC) progression is driven by cancer stem cells (CSCs), contributing to chemoresistance and tumor recurrence. N6-methyladenosine (m6A) modification plays a critical role in regulating CSC plasticity; however, the key m6A regulators in TNBC-CSCs remain poorly defined. Here, we identified IGF2BP3 as a dominant m6A reader enriched in TNBC-CSCs through transcriptomic analysis of the TCGA-BRCA dataset and validation by fluorescence-activated cell sorting (FACS).

Boron Cluster Functionalization: From Vertex-Specific Modifications to Boron Cage Extension.

Polycyclic molecular systems, which play a crucial role in the fields of materials science and pharmacology, typically consist of 2D extended organic aromatic rings. Extensive research has been conducted on conjugates formed through π-extension, leading to substantial advancements in the field. However, further exploration of novel conjugate systems is essential.

Experiences in practicing physical examination skills among undergraduate nursing students in clinical rotation based on the COM-B system: a descriptive qualitative study.

Purposes: To explore the experiences of Chinese undergraduate nursing students in practicing physical examination (PE) skills during clinical rotation.

Methods: A descriptive-qualitative approach was used. A total of 18 undergraduate nursing students from eight universities were interviewed.

[Effect of electroacupuncture on intestinal flora in COPD rats based on gut-lung axis theory].

Objective: To observe the effect of electroacupuncture (EA) on the intestinal flora in rats with chronic obstructive pulmonary disease (COPD) and explore its possible mechanism based on the gut-lung axis theory.

Methods: A total of 30 male SD rats of SPF grade were randomly divided into a normal control (NC) group, a model group and an EA group, 10 rats in each one. In the model group and the EA group, COPD model was established by intratracheal instillation of lipopolysaccharide combined with cigarette fumigation.

Genetic mapping of lifespan and mitochondrial stress response in .

The mitochondrial unfolded protein response (UPR) is one of the mito-nuclear regulatory circuits that restores mitochondrial function upon stress conditions, promoting metabolic health and longevity. However, the complex gene interactions that govern this pathway and its role in aging and healthspan remain to be fully elucidated. Here, we activated the UPR using doxycycline (Dox) in a genetically diverse population comprising 85 strains and observed large variation in Dox-induced lifespan extension across these strains.

A fish-specific antimicrobial peptide Piscidin2 inactivates MSRV and confers protection in largemouth bass.

Antimicrobial peptides (AMPs) represent an evolutionarily conserved component of innate immunity with broad-spectrum antimicrobial and antiviral activities. However, the antiviral potential of fish-specific piscidins against emerging aquatic viruses largely remains to be explored. In this study, we evaluated the antiviral properties of three piscidins (designated here as Piscidin1, Piscidin2 and Piscidin3) identified from largemouth bass () against rhabdovirus (MSRV), a major pathogen causing high mortality in farmed largemouth bass.

IGF2BP3 promotes autophagy-mediated TNBC metastasis via m6A-dependent, cap-independent c-Met translation.

Background: Metastatic tumors pose clinical treatment challenges due to their high adaptability to diverse environments. The cooperation of epigenetic modifications and metabolic adaptations enables tumor cells to dynamically adjust for survival in variable environments, which is crucial for tumor metastasis and worth exploring in depth.

Methods: RNA immunoprecipitation sequencing, transmission electron microscopy photograph and GFP-mCherry-LC3 fluorescence imaging were employed to reveal the role of insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) in triple-negative breast cancer (TNBC) cells.

Effects and Neuroimaging Findings of Acupuncture for Amnestic Mild Cognitive Impairment: A Randomized Controlled Trial.

Aim: Due to the limited evidence, the effects and neuroimaging mechanisms of acupuncture for amnestic mild cognitive impairment (aMCI) are still needed to investigate. Our objectives were to assess the effects and investigate its therapy-driven modification in functional neural response.

Methods: Right-handed aMCI patients were enrolled and randomly assigned to an acupuncture group (AG) or a waitlist control group (WG) in the randomized clinical trial using computer-generated randomization sequence method.

Sex-specific neurotoxicity induced by long-term bisphenol A exposure: Single-cell sequencing reveals dual mechanisms involving hippocampal gliosis and neuronal apoptosis.

Bisphenol A (BPA) is one of the most widely produced industrial chemicals globally. Exposure to BPA has been associated with neurobehavioral disorders and various other diseases. As an endocrine disruptor, BPA primarily binds to oestrogen receptors, thereby interfering with multiple neurological functions.

Instance-Based Transfer Learning with Similarity-Aware Subject Selection for Cross-Subject SSVEP-Based BCIs.

Steady-state visual evoked potential (SSVEP)-based brain-computer interfaces (BCIs) can achieve high recognition accuracy with sufficient training data. Transfer learning presents a promising solution to alleviate data requirements for the target subject by leveraging data from source subjects; however, effectively addressing individual variability among both target and source subjects remains a challenge. This paper proposes a novel transfer learning framework, termed instance-based task-related component analysis (iTRCA), which leverages knowledge from source subjects while considering their individual contributions.

Exploration of the varieties differences on the volatile and non-volatile metabolites of and utilizing electronic sensing evaluation and untargeted metabolomics analysis.

There are differences in flavor, efficacy, and price between the cardamom varieties (H) and (R). It is crucial to distinguish their bioactivities and metabolites, given their high economic value and dietary frequency. This study determined total phenolic content (TPC), total flavonoid content (TFC), and in vitro/cellular antioxidant activities of R and H.

Structural and functional characterization of human SLFN14.

The Schlafen (SLFN) family of proteins are a group of DNA/RNA processing enzymes with emerging importance in human health and disease, where their functions are implicated in a variety of immunological and anti-tumor processes. Here, we present the cryo-electron microscopy structure of full-length human SLFN14, a member with antiviral activity and linked to an inherited bleeding disorder. SLFN14 is composed of an RNase domain, a SWADL domain, and a two-lobe helicase domain.

Quinazoline Alkaloids for Plant Protection: Design, Synthesis, and Biological Evaluation of Novel Deoxyvasicinone Derivatives.

For many years, plant diseases brought about by viruses plus fungi have led to significant declines in crop yield as well as quality, highlighting the urgent need for the development of effective antiviral as well as fungicidal agents. Quinazolinone alkaloids have an important role in drug research and development (R&D). Herein, alkaloid deoxyvasicinone was chosen as the mother structure; deoxyvasicinone derivatives were designed, prepared, and evaluated for their antiviral and antifungal activities.

ASB7 is a negative regulator of H3K9me3 homeostasis.

The maintenance of histone H3 lysine 9 trimethylation (H3K9me3) involves the recognition of preexisting modifications by heterochromatin protein 1 (HP1), which recruits the methyltransferase suppressor of variegation 3-9 homolog 1 (SUV39H1) to methylate the adjacent newly incorporated histones, establishing a positive feedback loop. However, how this positive feedback is restricted to maintain H3K9me3 homeostasis remains largely unknown. We performed an unbiased genome-scale CRISPR-Cas9 screen and identified CUL5 E3 ubiquitin ligase as a negative regulator of H3K9me3.

Mental health service utilization among pregnant and postpartum women: status, determinants, and insights from a mixed-methods study.

Background: There is an increased risk of mental health disorders during pregnancy and postpartum period. However, care-seeking behaviors following positive screenings for maternal mental health disorders are poorly understood. Our study aimed to understand the rate of service utilization and determinants impacting the use of maternal mental health services.

Deep learning radiomics of left atrial appendage features for predicting atrial fibrillation recurrence.

Background: Structural remodeling of the left atrial appendage (LAA) is characteristic of atrial fibrillation (AF), and LAA morphology impacts radiofrequency catheter ablation (RFCA) outcomes. In this study, we aimed to develop and validate a predictive model for AF ablation outcomes using LAA morphological features, deep learning (DL) radiomics, and clinical variables.

Methods: In this multicenter retrospective study, 480 consecutive patients who underwent RFCA for AF at three tertiary hospitals between January 2016 and December 2022 were analyzed, with follow-up through December 2023.

Exploring the Role of Circadian Rhythm-Related Genes in the Identification of Sepsis Subtypes and the Construction of Diagnostic Models Based on RNA-seq and scRNA-seq.

Sepsis is a severe systemic response to infection that may lead to the dysfunction of multiple organ systems and may even be life-threatening. Circadian rhythm-related genes (CRDRGs) regulate the circadian clock and affect many physiological processes, including immune responses. In patients with sepsis, circadian rhythms may be disrupted, thus leading to problems such as immune responses.

Acupuncture Modulates Spatiotemporal Neuronal Dynamics in Mild Cognitive Impairment: A Protocol for Simultaneous EEG-fMRI Study.

Background: Mild cognitive impairment (MCI) is characterized by abnormal changes in spatiotemporal neuronal specificity responses. Simultaneous electroencephalogram (EEG)-functional magnetic resonance imaging (fMRI) offers a novel approach to measure these changes. Emerging evidence suggests that acupuncture may enhance cognitive function by modulating spatial or temporal central activity in individuals with MCI.