[Characterization of hippocampal components of Danzhi Xiaoyao Formula based on HPLC-Q-TOF-MS/MS and network pharmacology and assessment of its therapeutic potential for nervous system diseases].

In this study, the pharmacodynamic components and potential pharmacological functions of Danzhi Xiaoyao Formula in treating nervous system diseases were investigated by hippocampal component characterization and network pharmacology. After rats were administrated with Danzhi Xiaoyao Formula by gavage, high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(HPLC-Q-TOF-MS/MS) was employed to explore the components in the hippocampus of rats. Fifty-seven components were identified in the hippocampus of rats by comparing the extract of Danzhi Xiaoyao Formula, herbal components in the hippocampus after administration, and blank samples.

Investigation on Reactivity and Selectivity of Electrocatalytic CO Reduction in Photochemically Synthesized Ag and Alloyed AgCu and AgCu Nanoclusters.

Atomically precise nanoclusters are desirable for understanding the structure-property relationships in the electrocatalytic CO reduction reaction (eCORR), but suitable related models are lacking, especially those of low- or zerovalent noble metal nanoclusters and their alloyed analogues. We first developed a photochemical method toward silver nanocluster Ag(4- BuPhC≡C)(Dpppe)(SbF) ( -) and then related copper-doped alloyed nanocluster AgCu(4- BuPhC≡C)(Dpppe)Cl(SbF) ( -). Herein, we present a larger alloyed nanocluster, AgCu(4- BuPhC≡C)(Dpppe)(SbF) ( -) and investigate the relationship between the structures and the eCORR performance of those related nanoclusters.

The basic leucine zipper transcription factor TabZIP156 interacts with jasmonate ZIM-domain protein TaJAZ3-2A to regulate drought response in wheat.

Drought represents a major abiotic factor that restricts crop yields worldwide. Previous studies have demonstrated that the wheat bZIP transcription factor TabZIP156 plays a vital role in enhancing drought tolerance. However, the drought tolerance mechanism of TabZIP156 in wheat remains unclear.

MYOD represses gene expression from non-E-box motifs.

We report here on the identification of a previously unrecognized property of MYOD as a repressor of gene expression via E-box-independent chromatin binding during the process of somatic cell -differentiation into skeletal muscle. When ectopically expressed in proliferating human fibroblasts or endogenously induced in activated muscle stem cells (MuSCs), MYOD was detected at accessible regulatory elements of expressed genes, invariably leading to reduced chromatin accessibility and gene repression. At variance with conventional E-box-driven increased chromatin accessibility and H3K27 acetylation at previously silent loci of MYOD-activated genes, MYOD-mediated chromatin compaction and repression of transcription was associated with high occurrence of non-E-box motifs and did not lead to reduced levels of H3K27ac but coincided with reduced levels of H4 acetyl-methyl lysine modification (Kacme).

Fabrication of thermo-sensitive hydrogels based on the Diels-Alder reaction and study on their biosafety.

The goal of this study is to develop a novel injectable hydrogel, referred to as PPMF, and evaluate its biosafety profile. The PPMF polymer, which serves as the gelation precursor, was synthesized through a redox radical polymerization and amidation process. The molecular structures of the synthesized polymers were thoroughly characterized using H nuclear magnetic resonance (H NMR) and Fourier transform infrared spectroscopy (FTIR).

Dual regulation of FZD1/7 by IGF2BP3 enhances stem-like properties and carboplatin resistance in triple-negative breast cancer.

Triple-negative breast cancer (TNBC) progression is driven by cancer stem cells (CSCs), contributing to chemoresistance and tumor recurrence. N6-methyladenosine (m6A) modification plays a critical role in regulating CSC plasticity; however, the key m6A regulators in TNBC-CSCs remain poorly defined. Here, we identified IGF2BP3 as a dominant m6A reader enriched in TNBC-CSCs through transcriptomic analysis of the TCGA-BRCA dataset and validation by fluorescence-activated cell sorting (FACS).

National evaluations of pharmaceutical and personal care products in Chinese landfill leachate: non-negligible socio-economic and rainfall status.

Pharmaceuticals and personal care products (PPCPs) have received widespread attention owing to their negative effects on the health of ecosystems. While numerous studies monitored the usage of PPCPs, and ignored the systematical investigation of the discarded PPCPs. Here, we collected leachate from 73 landfills across China and detected 52 target PPCPs.

Spatio-temporal variations and trade-offs/synergies of typical ecosystem services in Ordos City.

Ordos City is located within the Yellow River 'Ji Zi Bend'. Clarifying the spatio-temporal variations and trade-offs/synergies of ecosystem services is of great significance for ecological protection and high-quality development of the Yellow River Basin. Based on the InVEST model and remote sensing data, we evaluated the ecosystem services of soil conservation, carbon storage, water conservation, and net primary productivity (NPP) in Ordos City from 2000 to 2020, and analyzed their spatio-temporal variations.

PGC-1α Expands Neural Precursor Pool and Facilitates Cognitive Recovery Within AD Hippocampus Through the Regulation of Mitochondrial Dynamics.

The dysfunction in learning and memory observed in Alzheimer's disease (AD) is strongly associated with impaired neurogenesis in the hippocampal region. As research on adult neurogenesis advances, it becomes increasingly crucial to identify potential targets for interventions aimed at enhancing endogenous neurogenesis and promoting functional recovery in AD patients. Our previous studies have demonstrated the potential of peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) in mitigating the pathological abnormalities associated with AD.

The effect of non-antibiotic pharmaceuticals and personal care products on antibiotic resistance genes in landfill refuse should not be underestimated.

Municipal landfills serve as significant reservoirs for pharmaceuticals and personal care products (PPCPs) as well as antibiotic resistance genes (ARGs). While most previous studies have focused on antibiotics, there is limited information regarding the occurrence of non-antibiotic PPCPs in landfill refuse and their impact on ARGs. This study systematically investigated the occurrence and evolutionary characteristics of 35 PPCPs, 15 ARGs, and 3 mobile genetic elements (MGEs) in landfill refuse of varying ages.

Hepatitis B virus core protein promotes liver cancer progression by stabilizing CANX and suppressing IRF7 transcription.

Hepatitis B virus (HBV) infection is a vital risk factor for the development of liver cancer. HBV core protein (HBC) contributes to the tumorigenesis induced by the virus. How HBC regulates liver cancer progress has not been well elucidated yet.

Multiomic profiling reveals that prostaglandin E2 reverses aged muscle stem cell dysfunction, leading to increased regeneration and strength.

Repair of muscle damage declines with age due to the accumulation of dysfunctional muscle stem cells (MuSCs). Here, we uncover that aged MuSCs have blunted prostaglandin E2 (PGE2)-EP4 receptor signaling, which causes precocious commitment and mitotic catastrophe. Treatment with PGE2 alters chromatin accessibility and overcomes the dysfunctional aged MuSC fate trajectory, increasing viability and triggering cell cycle re-entry.

Disabling PSGL-1 abrogates immune suppression and resistance to PD-1 blockade in pancreatic cancer.

Unlabelled: Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer for which there is a critical need to identify novel therapeutic targets. Herein we define PSGL-1 as a checkpoint inhibitor using a syngeneic orthotopic model of PDAC. As with PDAC patients, CD8 T cells within murine PDAC tumors expressed high levels of PSGL-1.

Collective Optical Properties of Moiré Excitons.

We propose that excitons in moiré transition metal dichalcogenide bilayers offer a promising platform for investigating collective radiative properties. While some of these optical properties resemble those of cold atom arrays, moiré excitons extend to the deep subwavelength limit, beyond the reach of current optical lattice experiments. Remarkably, we show that the collective optical properties can be exploited to probe certain correlated electron states without requiring subwavelength spatial resolution.

Landfill depths alter microbial community structure and ecological assembly by affecting δC and organic matter.

Microorganisms are crucial for the degradation of organic matter during landfill. However, the processes of microbial community assembly and ecological niche in landfill are poorly understood. Here, the mechanisms underlying microbial assembly in landfill were investigated based on neutral theory, niche distribution and network analysis.

FOXG1 Improves Cognitive Function in Alzheimer's Disease by Promoting Endogenous Neurogenesis.

Strategies aimed at enhancing the capacity of neural stem cells (NSCs) to generate multipotential, proliferative, and migratory cell populations capable of efficient neuronal differentiation are crucial for structural repair following neurodegenerative damage. The role of Forkhead-box gene 1 (FOXG1) in pattern formation, cell proliferation, and specification has been established. However, its involvement in Alzheimer's disease (AD) remains largely unknown.

Electrosynthesis of Atomically Precise Au Nanoclusters.

Innovation in synthesis methodologies is crucial for advancing the discovery of new materials. This work reports the electrosynthesis of a [Au(4-BuPhC≡C)(Dppe)]Cl nanocluster (Au NC) protected by alkynyl and phosphine ligands. From simple precursor, HAuCl and ligands, the whole synthesis is driven by a constant potential in single electrolytic cell.

The community succession mechanisms and interactive dynamics of microorganisms under high salinity and alkalinity conditions during composting.

Microorganisms drive organic matter degradation and humification during composting. However, the mechanisms underlying microbial community succession and their interactions under saline-alkali stress are poorly understood. In this study, we investigated the microbial community assembly processes and microbial niche dynamics during composting in the high-saline-alkaline region.

[Efficacy and Safety of DCAG Regimen in Patients with Relapsed/Refractory Acute Myeloid Leukemia].

Objective: To evaluate the efficacy and safety of DCAG (decitabine, cytarabine, anthracyclines, and granulocyte colony-stimulating factor) regimen in the treatment of patients with relapsed/refractory (R/R) acute myeloid leukemia (AML).

Methods: The clinical data of 64 R/R AML patients received treatment at Chinese PLA General Hospital from January 1st, 2012 to December 31st, 2022 were retrospectively analyzed. Primary endpoints included efficacy measured by overall response rate (ORR) and safety.

Comparative study of gut microbiota reveals the adaptive strategies of gibbons living in suboptimal habitats.

Wild animals face numerous challenges in less ideal habitats, including the lack of food as well as changes in diet. Understanding how the gut microbiomes of wild animals adapt to changes in food resources within suboptimal habitats is critical for their survival. Therefore, we conducted a longitudinal sampling of three gibbon species living in high-quality (Nomascus hainanus) and suboptimal (Nomascus concolor and Hoolock tianxing) habitats to address the dynamics of gut microbiome assembly over one year.

Construction of a prognostic model for gastric cancer based on immune infiltration and microenvironment, and exploration of MEF2C gene function.

Background: Advanced gastric cancer (GC) exhibits a high recurrence rate and a dismal prognosis. Myocyte enhancer factor 2c (MEF2C) was found to contribute to the development of various types of cancer. Therefore, our aim is to develop a prognostic model that predicts the prognosis of GC patients and initially explore the role of MEF2C in immunotherapy for GC.

Effect of BSSRO on disc-condyle relationship of the temporomandibular joint in skeletal Class III malocclusion patients.

Purpose: To analyze dynamic and static changes in the disc-condyle relationship in patients with skeletal Class III malocclusion after orthognathic surgery.

Methods: The surgical group comprised 30 patients with skeletal Class III malocclusion, and the magnetic resonance imaging and mandibular movement data were obtained at T0 (preoperatively), T1 (3 months postoperatively), and T2 (at the end of orthodontic treatment). The control group included 20 patients with normal occlusion, and the mandibular movement data were recorded.

[Electroacupuncture of "Jiaji" (EX-B2) inhibits inflammatory response by regulating HMGB1/TLR4/NF-κB signaling pathway in rats with spinal cord injury].

Objectives: To observe the effect of electroacupuncture (EA) of "Jiaji" (EX-B2) on expression of high mobility group box 1 (HMGB1)/Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) related proteins and inflammation-related factors in spinal cord injury (SCI) rats, so as to explore the dynamic process of inhibiting inflammatory response in rats with SCI, and to provide a new idea and theoretical basis of molecular biology for the treatment of SCI.

Methods: Fifty-four SD rats were randomly divided into sham operation, model, and EA groups (=18 in each group), which were further divided into 3 d, 7 d and 14 d subgroups, with 6 rats at each time point. The SCI model was established according to the Allen's method.