Nonlinear multi-head cross-attention network and programmable gradient information for gaze estimation.

Gaze estimation is an important indicator of human behavior that can be used for human assistance. Recent gaze estimation methods are primarily based on convolutional neural networks (CNNs) or attention Transformers. However, CNNs extract a limited local context while losing important global information, whereas attention mechanisms exhibit low utilization of multiscale hybrid features.

Pomelo Peel and Soybean Meal Fermented Compound as Feedstuff for Large Yellow Croaker (): A Study on Growth and Intestinal Health.

Pomelo peel is a main by-product of pomelo consumption, while most of the by-products are discarded as wastes with environmental threats. Indeed, it contains many nutrients and bioactive ingredients, which makes it a promising source of aquatic feedstuff. In this study, a 56-day feeding trial was set out to assess the dietary application of pomelo peel and soybean meal (SBM) (1:4 ratio) fermented compound (PSFC) in large yellow croaker ().

Dopaminergic system disruption induced by envrionmentally-realistic glyphosate leads to behavioral alteration in crayfish, Procambarus clarkii.

Glyphosate, a widely used herbicide, is frequently detected in freshwater systems, posing potential risks to non-target aquatic species. This study investigates the effects of environmentally realistic glyphosate concentrations (0.1, 1, and 10 µg/L) on the behavior, dopamine regulation, and oxidative stress in the red swamp crayfish (Procambarus clarkii), a key species in freshwater ecosystems and also an important commercial species in China.

MAPK-CncC Signaling Pathways Regulate the Antitoxic Response to Avermectin-Induced Oxidative Stress in Juvenile Chinese Mitten Crab, .

This study delves into the adverse effects of AVM, emphasizing oxidative stress induction in the Chinese mitten crab, , and the role of the MAPK-CncC signaling pathway in mediating the antioxidative response. Our findings reveal a dose-dependent impairment in growth performance, alongside occurrence of oxidative stress. The activity of CAT and superoxide dismutase increased significantly in all treatments (0.

Application of Natural Antioxidants as Feed Additives in Aquaculture: A Review.

Oxidative stress, a critical factor affecting the health and productivity of aquatic organisms, arises from the imbalance between reactive oxygen species (ROS) production and antioxidant defenses. In aquaculture, natural antioxidants have gained increasing attention as sustainable feed additives to mitigate oxidative damage, enhance immune responses, and improve overall growth performance. This review provides a comprehensive synthesis of the antioxidative mechanisms of key natural antioxidants, including carotenoids, polysaccharides, vitamins, polyphenols, and flavonoids.

Effects of glyphosate on neurotoxicity, oxidative stress and immune suppression in red swamp crayfish, Procambarus Clarkii.

Glyphosate, a prevalent herbicide, has raised concerns due to its potential ecological impact, especially on aquatic ecosystems. While it is crucial for managing agricultural productivity, its inadvertent effects on non-target aquatic species like the red swamp crayfish, Procambarus clarkii, are not fully understood. In the present study, the neurotoxicity, oxidative stress, and immune suppression of glyphosate on P.

Abamectin-induced behavioral alterations link to energy metabolism disorder and ferroptosis via oxidative stress in Chinese mitten crab, Eriocheir sinensis.

The increasing application of abamectin (ABM) in agriculture has raised concerns regarding its environmental safety and potential adverse effects on aquatic environment safety. In the present study, the toxic effects of ABM exposure on the adult Chinese mitten crab, Eriocheir sinensis were investigated, with a focus on locomotion impairment, behavioral changes, oxidative stress, energy metabolism disruption, and ferroptosis. Crabs were exposed to sublethal concentrations of ABM at 2, 20 and 200 μg/L.

Reactive Oxygen Species Signaling and Oxidative Stress: Transcriptional Regulation and Evolution.

Since the evolution of the aerobic metabolism, reactive oxygen species (ROS) have represented significant challenges to diverse life forms. In recent decades, increasing knowledge has revealed a dual role for ROS in cell physiology, showing they serve as a major source of cellular damage while also functioning as important signaling molecules in various biological processes. Our understanding of ROS homeostasis and ROS-mediated cellular signaling pathways has presumed that they are ancient and highly conserved mechanisms shared by most organisms.

Effects of chlorantraniliprole-based pesticide on transcriptional response and gut microbiota of the crucian carp, Carassius carassius.

Chlorantraniliprole (CAP) is a presentative diamide pesticide utilized in agricultural area and as well as rice-fish co-culture system for pest control. However, the understanding of toxic effects of CAP on fish species is still incomplete. In the present study, we performed an integrated study of the acute toxicity and bioaccumulation of CAP on the crucian carp, Carassius carassius, a fish species widely distributed in freshwater area in China and commonly farmed in the rice-fish co-culture systems.

Cytotoxicity induced by abamectin in hepatopancreas cells of Chinese mitten crab, Eriocheir sinensis: An in vitro assay.

Toxic effects of abamectin on non-target aquatic organisms have been well documented due to its extensive use in both agricultural and aquacultural areas. However, knowledge of the abamectin induced cytotoxicity in crustacean hepatopancreas is still incomplete. In this study, we investigated the cytotoxic effects of abamectin on hepatopancreas cells of Chinese mitten crab, Eriocheir sinensis by an in vitro assay.

Prolonged darkness attenuates imidacloprid toxicity through the brain-gut-microbiome axis in zebrafish, Danio rerio.

The present study investigated the toxic effects of IMI on brain and gut of zebrafish (Danio rerio) by a combination of transcriptome and microbiome analysis. In addition, the involvement of light/dark period was also evaluated. An acute toxic test was conducted on adult zebrafish weighing 0.

Development and application of an indirect ELISA to detect antibodies to in cattle based on a chimeric protein rSRS2-SAG1-GRA7.

is an important apicomplexan parasite causing neosporosis in cattle. The disease is recognized as one of the most important cause of reproductive problems and abortion in cattle worldwide. In this context, we developed an indirect enzyme-linked immunosorbent assays (ELISA) with chimeric protein rSRS2-SAG1-GRA7 to diagnose antibodies to -infection.

A New Perspective on the Antimicrobial Mechanism of Berberine Hydrochloride Against Revealed by Untargeted Metabolomic Studies.

Berberine hydrochloride (BBR) is a natural product widely used in clinical medicine and animal production. It has a variety of antimicrobial effects, but its complex antimicrobial mechanism has not been clarified. This study aimed to discover the metabolic markers and gain a new perspective on the antibacterial mechanism of BBR.

Revitalizing mouse diphyodontic dentition formation by inhibiting the sonic hedgehog signaling pathway.

Background: Tooth regeneration depends on the longevity of the dental epithelial lamina. However, the exact mechanism of dental lamina regression has not yet been clarified. To explore the role of the Sonic hedgehog (Shh) signaling pathway in regression process of the rudimentary successional dental lamina (RSDL) in mice, we orally administered a single dose of a Shh signaling pathway inhibitor to pregnant mice between embryonic day 13.

Osteogenic and angiogenic profiles of the palatal process of the maxilla and the palatal process of the palatine bone.

Hard palate consists anteriorly of the palatal process of the maxilla (ppmx) and posteriorly of the palatal process of the palatine (ppp). Currently, palatal osteogenesis is receiving increasing attention. This is the first study to provide an overview of the osteogenesis process of the mouse hard palate.

Imidacloprid induces locomotion impairment of the freshwater crayfish, Procambarus clarkii via neurotoxicity and oxidative stress in digestive system.

Imidacloprid (IMI) is used in integrated farming like the rice-crayfish co-culture system to prevent water weevil. However, the toxic effect of IMI on the freshwater crayfish Procambarus clarkii is unknown. In the current study, the effects of IMI on the locomotion, antioxidative status, digestion and intestinal microbiota of P.

Defining the critical period of hedgehog pathway inhibitor-induced cranial base dysplasia in mice.

Background: The hedgehog signaling pathway is critical for developmental patterning of the limb, craniofacial and axial skeleton. Disruption of this pathway in mice leads to a series of structural malformations, but the exact role and critical period of the Hh pathway in the early development of the cranial base have been rarely described.

Results: Embryos exposed to vismodegib from E7.

DNA damage, immunotoxicity, and neurotoxicity induced by deltamethrin on the freshwater crayfish, Procambarus clarkii.

Pyrethroid pesticides are applied to both agricultural and aquacultural industries for pest control. However, information of their impact on the commercial important freshwater crayfish, Procambarus clarkii is scarce. Therefore, the present study aimed to characterize to effects of a commonly used pyrethroid pesticide, deltamethrin on DNA damage, immune response, and neurotoxicity in P.

Abamectin at environmentally-realistic concentrations cause oxidative stress and genotoxic damage in juvenile fish (Schizothorax prenanti).

Abamectin (ABM) has been extensively used in Chinese aquaculture systems for parasite control, but no information is available regarding its effects on the important freshwater commercial fish species Schizothorax prenanti. We performed an acute toxicity test to determine the effects of ABM on S. prenanti, and the 48- and 96-h median lethal concentration values were 33.

Effects of imidacloprid on the oxidative stress, detoxification and gut microbiota of Chinese mitten crab, Eriocheir sinensis.

Imidacloprid (IMI) is used in integrated aquaculture systems for pest control and the toxicity of IMI to non-target aquatic animals such as fish and microcrustaceans has been recognised. However, knowledge about the toxic effect of IMI on commercial crabs is still scarce. In the present study, effects of IMI on the acute toxicity, antioxidative status, detoxification systems and gut microbiota in Chinese mitten crab, Erocheir sinensis were investigated.

Cytotoxicity induced by abamectin exposure in haemocytes of Chinese mitten crab, Eriocheir sinensis.

Abamectin is widely utilized in both agricultural and aquaculture areas in China for pest control. However, information about toxic effects of abamectin on non-target aquatic organisms is still incomplete. The Chinese mitten crab, Erocheir sinensis has been extensively bred in the rice-crab co-culture system for years, resulting in the frequent exposure to pesticides including abamectin.

Avermectin induces the oxidative stress, genotoxicity, and immunological responses in the Chinese Mitten Crab, Eriocheir sinensis.

Avermectin is commonly used in aquaculture systems for pest control in recent decades in China. However, no information is provided for the toxic effect to the important commercial species, Chinese mitten crab, Eriocheir sinensis. To investigate the aquatic toxicity of avermectin, an acute toxic test was performed in this study.

Immune response to abamectin-induced oxidative stress in Chinese mitten crab, Eriocheir sinensis.

It is known that abamectin (ABM) inflicts oxidative damage on aquatic animals; however, knowledge about the immune response under pesticide-induced oxidative stress is incomplete. In the present study, several cellular and humoral immune parameters, including total haemocyte counts (THC), lysosomal membrane stability (LMS), activities of acid phosphatase (ACP), alkaline phosphatase (AKP) and lysozyme (LZM) were investigated to reveal the effects of ABM exposure on the immune defence mechanisms of the important freshwater crab, Erocheir sinensis. According to the results, a significant increase of THC was found in low concentration groups (0.

Oxidative stress, immunological response, and heat shock proteins induction in the Chinese Mitten Crab, Eriocheir sinensis following avermectin exposure.

In this study, the Chinese mitten crabs, Eriocheir sinensis were exposed to avermectin at 0.03, 0.06, 0.

Effects of deltamethrin on the antioxidant defense and heat shock protein expression in Chinese mitten crab, Eriocheir sinensis.

In the present study, the status of antioxidant response and molecular regulation in Chinese mitten crab, Eriocheir sinensis under the exposure of synthetic pyrethroid deltamethrin were investigated by means of measuring the antioxidative enzyme activity and relative mRNA expression of heat shock proteins (HSPs) in hepatopancreas. The results showed that activity of superoxide dismutase (SOD) and catalase (CAT) decreased remarkably in all treatments except the SOD activity at concentration of 0.073 μg/L.