Direct Probing the Electrical Double Layer at Graphite/Nafion Interface via Depth Profiling APXPS.

Understanding the electrical double layer (EDL) is fundamental for enhancing the efficiency, capacity, and stability of electrochemical systems. The EDL at the electrode/polyelectrolyte interface exhibits significantly different properties with a more complex structure compared to liquid electrolyte systems. Characterizing this intricate interface experimentally remains a major challenge.

Chromosome-level genome assembly of the caddisfly Stenopsyche angustata (Insecta: Trichoptera).

Stenopsyche angustata, a species within the diverse order Trichoptera, is widely distributed across freshwater environments and exhibits unique ecological traits that make it an ideal subject for studying adaptive evolution. In this study, we employed Illumina second-generation sequencing, PacBio third-generation sequencing, along with high-throughput chromosome conformation capture (Hi-C) technologies to generate raw sequencing data and construct chromosome-level genome assemblies of S. angustata.

A high-sensitivity and clogging-free microfluidic impedance flow cytometer based on three-dimensional hydrodynamic focusing.

Microfluidic impedance flow cytometry has functioned as an enabling instrument in single-cell analysis, which, however, suffers from the limiting tradeoff between high sensitivity and clogging-free operation. In order to address this issue, this study presented a microfluidic impedance flow cytometer based on three-dimensional (3D) hydrodynamic focusing, in which the crossflow of conductive sample fluids and insulating sheath fluids was leveraged to centralize and restrict electric field lines to the sample fluid, thereby achieving high impedance sensitivity of single cells without the concern of channel blockage. Different from conventional impedance flow cytometry, in this study, impedance amplitude dips (rather than pulse singles) generated by single microparticles traveling through the 3D hydrodynamic focusing region were experimentally validated using microbeads.

Transcriptomic profiling reveals tetrabromobisphenol A-induced dysregulation of hepatic lipid homeostasis in common carp (Cyprinus carpio).

TBBPA, a ubiquitously distributed brominated flame retardant, poses significant ecological risks due to its bioaccumulative potential and toxicity. Previous investigations have predominantly focused on untargeted metabolomics and lipidomics to characterize the effects of TBBPA on metabolic end products. However, limited data are available regarding the mechanisms underlying TBBPA-induced disruption of lipid metabolism in aquatic organisms.

Inhibitory leukocyte immunoglobulin-like receptors, subfamily B (LILRBs) in human diseases: structure, roles, mechanisms, and clinical applications.

Leukocyte immunoglobulin-like receptors, subfamily B (LILRBs), are a class of critical immunosuppressive receptors that contribute to immune homeostasis by transmitting suppressive signals upon binding to ligands such as major histocompatibility complex class I molecules. They play key roles in modulating both innate and adaptive immune responses. This review summarizes the structural features, ligand interactions, signaling pathways, and expression regulation of LILRBs, and discusses their roles in immune cell function and disease progression, particularly in the tumor microenvironment.

Microbial dysbiosis induced in Cyprinus carpio by tetrabromobisphenol A exposure: Mediation through gut barrier impairment and oxidative stress.

The frequent detection of Tetrabromobisphenol A (TBBPA) in environmental media has elicited considerable scientific concern. The ecotoxicological impacts of TBBPA on intestinal health in the common carp (Cyprinus carpio) were systematically investigated through integrated histopathological, biochemical, and metagenomic analyses. Fish were exposed to environmentally relevant TBBPA concentrations (0, 0.

Urinary elementomic analysis indicates aluminum as a potential urinary biomarker of sarcopenia in the older adults.

Sarcopenia is characterized by aging-related progressive loss of muscle mass and function; however, the specific and sensitive biomarkers are still limited. Biometals and trace elements provide a potential connection linking the environment and lifestyle to pathological processes of sarcopenia. The aim of the present study was to investigate the relationship between urinary trace elements levels and the presence of sarcopenia.

16S rDNA sequencing and targeted metabolomics reveal rhizosphere microbial diversity and flavonoid dynamics in Scutellaria baicalensis Georgi.

Aims: Scutellaria baicalensis Georgi is a commonly utilized bulk Chinese medicinal material in China. In clinical practice, it is often divided into ZiQin and KuQin according to their growth years and different medicinal purposes. Extensive herbal research and pharmacological studies have demonstrated distinct effects of ZiQin and KuQin.

Myrtenol ameliorates ulcerative colitis by modulating ANXA1/PINK1/Parkin-mediated mitophagy.

Ulcerative colitis (UC) is a chronic inflammatory disorder characterized by recurrent and intermittent episodes of inflammation. (-)-Myrtenol (MYR) has been shown to exhibit anti-inflammatory, antioxidant, and gastroprotective properties; however, its therapeutic potential in UC remains unexplored. In this study, we established in vitro UC models using lipopolysaccharide (LPS)-induced Caco-2 cells and in vivo models using dextran sulfate sodium (DSS)-induced mice to investigate the effects of MYR on UC.

Use of extracorporeal membrane oxygenation in adult trauma patients with refractory acute cardiopulmonary failure: guideline from the Chinese society of extracorporeal life support 2025.

Background: Adult trauma patients with refractory acute cardiopulmonary failure suffer from high morbidity and mortality. In the past decade, a growing body of researches has shown survival benefits of extracorporeal membrane oxygenation (ECMO) in trauma patients who fail to respond to optimal damage control resuscitation (DCR), and there is an opportunity to formulate clinical practice guidelines to guide clinicians in implementing trauma ECMO at the bedside.

Methods: The Chinese Society of Extracorporeal Life Support (CSECLS) convened a domestic panel of interdisciplinary experts to develop this guideline, adhering to the principles of the World Health Organization (WHO) Manual for Guideline Development and the policy of conflict of interest.

Multi-Omics Analysis and Real-World Data Validation of Serine Metabolism-Related Genes in Colorectal Cancer.

Serine metabolism plays a pivotal role in cancer progression by supporting essential biosynthetic pathways and energy production. Exploring the intricacies of serine metabolism in cancer may uncover novel therapeutic opportunities. This study presents a comprehensive pan-cancer analysis of serine metabolism-related genes (SMGs), with a particular emphasis on colorectal cancer (CRC), to elucidate their expression patterns, genetic alterations and clinical significance.

Column-free purification of functional HIV-1 capsid protein and its application in assembly and inhibitor assays.

The HIV-1 capsid protein (CA) is essential for viral replication and serves as a validated antiviral drug target. Traditional purification of CA relies on multi-step chromatographic protocols, which are time-consuming and labor-intensive. In this study, we established a rapid, column-free purification strategy using a cleavable self-aggregating tag (cSAT) to produce functional wild-type CA protein from E.

Targeted suppression of glioma by ultralow-dose x-ray-induced photodynamic therapy and gold-based nanoclusters in preclinical models.

The diffuse and infiltrative nature of high-grade gliomas poses ongoing challenges in treatment and management. Radiotherapy is an important glioma treatment, with a standard radiotherapy dose of 60 gray. However, high-dose radiotherapy is associated with radiation-induced side effects on normal tissue.

Occurrence, distribution, and potential risk of 61 glucocorticoids in soils in Shandong Province, China.

Glucocorticoids (GCs) in the environment are of increasing concern due to the potent endocrine disrupting effects. However, most studies only explored their occurrences and behaviors in the aqueous phase, and few studies have investigated their concentrations and risks in soil. In this study, 61 natural and synthetic GCs were simultaneous analyzed, and their occurrences, spatial distribution and ecological risks in soil from 13 cities in Shandong Province, China were further discussed.

Exploring the role of inflammation and proinflammatory proteins in coagulopathy during venovenous extracorporeal membrane oxygenation.

Background: Coagulopathy frequently complicates venovenous extracorporeal membrane oxygenation (VV ECMO) procedures, yet its underlying mechanism remains elusive. This retrospective study aimed to identify factors contributing to coagulopathy during VV ECMO by analyzing clinical data and employing proteomic approaches. A prospective cohort was also examined for validation.

Thoracic paravertebral block versus local infiltration anesthesia for percutaneous kyphoplasty to treat osteoporotic vertebral compression fractures combined with intercostal neuralgia: a randomized controlled trial.

Background: Percutaneous kyphoplasty (PKP) is an effective treatment for osteoporotic vertebral compression fractures (OVCFs) and provides effective pain relief; however, its efficacy is questionable in patients with thoracic OVCFs combined with intercostal neuralgia (IN). This study aims to compare the efficacy and safety of thoracic paravertebral nerve block (TPVB) and local infiltration (LI) anesthesia for PKP to treat thoracic OVCFs combined with IN.

Methods: Patients with OVCFs combined with IN scheduled to undergo PKP between January 2021 and June 2022 were randomized into the following groups: TPVB and LI.

Clinicopathological and molecular characteristics of paediatric-type follicular lymphoma: an analysis of 39 cases.

Our aim was to investigate the clinicopathological characteristics of paediatric-type follicular lymphoma (PTFL). The clinicopathological data were collected from 39 patients with PTFL diagnosed at the Department of Pathology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, China, from January 2017 to September 2024, including the morphology, immunohistochemical (IHC) analysis, and Ig gene rearrangements detected by polymerase chain reaction (PCR). Fluorescence in situ hybridisation (FISH) was performed to detect breakages in BCL2, BCL6, MYC, and IRF4 genes.

Natural product sennoside B disrupts liquid-liquid phase separation of SARS-CoV-2 nucleocapsid protein by inhibiting its RNA-binding activity.

The nucleocapsid protein (NP) of SARS-CoV-2, an RNA-binding protein, is capable of undergoing liquid-liquid phase separation (LLPS) during viral infection, which plays a crucial role in virus assembly, replication, and immune regulation. In this study, we developed a homogeneous time-resolved fluorescence (HTRF) method for identifying inhibitors of the SARS-CoV-2 NP-RNA interaction. Using this HTRF-based approach, we identified two natural products, sennoside A and sennoside B, as effective blockers of this interaction.

Trials of a drug release platform in near-spherical porous NiTi alloys containing a thermosensitive hydrogel as the inner coating.

This study presents a preliminary investigation of porous nickel-titanium (NiTi) materials with controllable porosity fabricated through metal injection molding combined with the powder space-holder method (MIM-PSH). The thermosensitive hydrogel Pluronic F-127 was utilized as a drug carrier to load the anti-proliferative drug rapamycin, resulting in porous NiTi-hydrogel composite materials for controlled drug release. By tuning the NiTi matrix porosity (0%, 20%, and 40%), the system achieved precise modulation of drug loading capacity and release kinetics.

Cloning, Expression, and Functional Characterization of three 6-hydroxyalizarin Glycosyltransferases from Rheum palmatum.

Glycosyltransferases are key enzymes responsible for the glycosylation of natural products in plants. The roots and rhizomes of Rheum palmatum Diels, commonly known as rhubarb, are well-established in traditional Chinese medicine and are rich in diverse glycoside natural products. Despite this, no glycosyltransferase from R.

Exosome biomarkers in breast cancer: Systematic review and meta-analysis.

Background: Breast cancer (BC) has become the primary cancer that threatens women's health and life expectancy. Early diagnosis is crucial for effective treatment and favourable prognosis. As a non-invasive and valuable liquid biopsy method, exosomes are promising for the diagnosis and prognosis of BC.

A Cytotoxic T Lymphocyte-Inspiring Microscale System for Cancer Immunotherapy.

Adoptive T cell therapy (ACT) is an emerging cancer immunotherapy undergoing clinical evaluation, showing significant promise in the treatment of solid tumors. However, the clinical translation of ACT is hindered by its time-, labor-, and financial-consuming procedures, heterogeneity of cytotoxic T lymphocytes (CTLs), and immunosuppressive tumor microenvironment. Herein, we have developed a bionic cytotoxic T lymphocyte-inspiring microscale system (CTLiMS) composed of mesoporous silica dioxide microspheres containing membrane-disrupting boron clusters (BICs) and proapoptotic monomethyl auristatin E (MMAE) peptides.

Adjusted glycated albumin is a novel indicator of glycemic control in patients with macroalbuminuria in diabetic kidney disease.

Glycated albumin (GA), a blood glucose monitoring biomarker, is impacted by variables such as albumin turnover and is not entirely relevant throughout diabetic kidney disease (DKD). There is insufficient data to routinely adjust GA measurements. We examined how albuminuria affected clinically measured GA (mGA) and adjusted GA (adjGA).

Effect of enhanced recovery after radiotherapy (ERAR) on the quality of life in patients with nasopharyngeal carcinoma after radiotherapy: A randomized controlled trial.

Nasopharyngeal carcinoma is prevalent in Southern China and Southeast Asia, which is effectively managed through chemoradiotherapy for locoregionally advanced cases (LA-NPC). Despite current guidelines endorsing various rehabilitation regimens for surgery, evidence supporting the benefits of rehabilitation during systemic radiotherapy is scarce. Thus we introduce the Enhanced Recovery After Radiotherapy (ERAR) concept.