Graphene oxide-based dual-color thin-film fluorescent nanolabels for enhanced immunochromatographic detection of pesticides.

Background: The excessive use of pesticide pollutants in agricultural production seriously threatens food safety. Traditional detection techniques are difficult to meet the detection requirements due to the complex sample pretreatment and high detection costs. The immunochromatography method (ICA) is simple to operate and fast, and is suitable for on-site rapid detection.

Exploration of preventive treatment for high risk patients with metachronous multiple esophageal squamous cell carcinoma: A case report.

Background: Metachronous multiple esophageal squamous cell carcinomas (ESCCs) may occur in some patients after endoscopic resection. Multiple dysplastic lesions in the esophagus increase risk of multiple squamous cell carcinomas (SCCs). Endoscopic imaging technology such as narrow band imaging (NBI), can detect early SCC.

Reclassification of Ming . 2012 as a later heterotypic synonym of (Nakamura 1987) Shida . 1997.

In this study, the taxonomic relationship between and was reassessed utilizing a polyphasic taxonomic approach. Molecular identification through 16S rRNA gene sequence analysis demonstrated that DSM 5050 exhibited the highest sequence similarities with LMG 31458 (99.57%), LMG 31460 (99.

[Clinical and histological evaluation of three-dimensional printing individualized titanium mesh for alveolar bone defect repair].

Objectives: To evaluate the osteogenic efficacy of three-dimensional printing individualized titanium mesh (3D-PITM) as a scaffold material in guided bone regeneration (GBR).

Methods: 1) Patients undergoing GBR for alveolar bone defects were enrolled as study subjects, and postoperative healing complications were recorded. 2) Postoperative cone beam computed tomography (CBCT) scans acquired at least 6 months post-surgery were used to calculate the percentage of actual bone formation volume.

Physiological and biochemical basis of flower coloration in Aquilegia oxysepala with a functional study of AoDFR08.

Background: , the most widespread columbine in northeast China, has considerable potential for future applications. It is imperative to elucidate the potential physiological and molecular mechanisms underlying flower coloration, which could facilitate the innovative breeding process of .

Results: A comprehensive analysis was conducted to elucidate the physio-biochemical basis of flower coloration in and .

Efficacy of 3D-Printed Bioactive Glass Tetrahedral Particles for Vertical Bone Regeneration: A Comparative Study.

This study was designed to systematically evaluate the osteogenic efficacy of 3D-printed tetrahedral bioactive glass particles in vertical bone regeneration and compare their performance with that of conventional bone substitute materials. In this investigation, 3D tetrahedral bioactive glass particles were fabricated using digital light processing (DLP) additive manufacturing technology. The structural integrity and chemical composition of the particles were characterized by scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), and X-ray diffraction (XRD) to confirm their conformity to design specifications.

A disproportionality analysis of adverse events caused by pexidartinib from the FDA adverse event reporting system.

Tenosynovial giant cell tumor (TGCT) is a rare neoplasm closely associated with dysregulation of the colony-stimulating factor 1(CSF1)/CSF1R signaling pathway, faces high recurrence rates despite surgical intervention, prompting exploration of CSF1R inhibitors like pexidartinib. This retrospective pharmacovigilance study analyzed pexidartinib-associated adverse events (AEs) from FDA Adverse Event Reporting System (FAERS) data (Q4 2019-Q3 2024), employing disproportionality analyses (ROR, PRR, BCPNN, EBGM) and sensitivity assessments to evaluate 844 reports. Hepatic events (46.

Protective Efficacy of Subunit Vaccine Expressing Rv0976c Against Tuberculosis.

Objectives: The construction of subunit vaccines based on antigens that can induce strong cellular immunity is a widely accepted strategy to develop new tuberculosis vaccines. This study screens immunogens with potential for subunit vaccine development from seven candidate antigens and then verifies their vaccine efficacy.

Design: C57BL/6 mice were immunized subcutaneously with purified PPE19, PPE50, FadD21, Rv1505c, Rv1506c, Rv2035, and Rv0976c proteins formulated with Freund's adjuvant to evaluate both the antigen-specific Th1 cellular immune responses and IgG level.

Genome-Wide Identification and Evolutionary Analysis of the GATA Transcription Factor Family in Nitrogen-Fixing Legumes.

GATA transcription factors are crucial for plant development and environmental responses, yet their roles in plant evolution and root nodule symbiosis are still not well understood. This study identified GATA genes across the genomes of 77 representative plant species, revealing that this gene family originated in Charophyta and significantly expanded in both gymnosperms and angiosperms. Phylogenetic analyses, along with examinations of conserved motifs and cis-regulatory elements in and , clearly demonstrated structural and functional divergence within the GATA family.

Ethacrynic acid regulates gentamicin ototoxicity via the blood-labyrinth barrier.

Gentamicin (GM), a widely used aminoglycoside antibiotic, has its clinical utility significantly limited by ototoxicity, which may be further exacerbated by co-administered drugs. This study systematically investigated the ototoxic mechanisms of GM combined with ethacrynic acid (EA) and the protective effects of N-acetylcysteine (NAC) using C57BL/6 J mice. Results revealed dose-dependent GM-induced ototoxicity.

Cepharanthine Enhances MHC-I Antigen Presentation and Anti-Tumor Immunity in Melanoma via Autophagy Inhibition.

Major histocompatibility complex class I (MHC-I)-mediated antigen presentation plays a pivotal role in anti-tumor immunity by enabling CD8 T cells to recognize and eliminate malignant cells. In melanoma, modulation of this pathway is critical for improving the efficacy of immunotherapies. Our study demonstrates that the natural compound Cepharanthine (CEP) exhibits notable antitumor activity by enhancing MHC-I-mediated antigen presentation.

The Class III peroxidase OsPrx20 is a key regulator of stress response and growth in rice.

In plants, reactive oxygen species (ROS) are maintained at strictly low intracellular levels, a prerequisite for their function as signals. Class III peroxidases serve as core regulators of intracellular ROS homeostasis, if environmental stimuli trigger long-term responses causing ROS accumulation. Here, we found a rice Class III peroxidase OsPrx20, which is a target of Ca/calmodulin-dependent protein kinase (OsDMI3) and is phosphorylated at its threonine (Thr)-244 site by OsDMI3, thereby positively regulating osmotic stress tolerance while negatively regulating blast resistance in rice.

Genetic basis of transmission of bla among foodborne Vibrio parahaemolyticus strains in China.

Carbapenem-resistant Vibrio parahaemolyticus has emerged and spread extensively in China, posing a substantial threat to food safety and human health. This study investigated the prevalence of carbapenem resistance in V. parahaemolyticus strains recovered from various types of food samples in China, and the underlying mechanisms of transmission of the bla gene harbored by such strains.

Biobased carbon quantum dots: An emerging nano-enabled paradigm for pesticide removal and sustainable plant protection.

Owing to their tunable surface chemistry, high biocompatibility and unique optical properties, carbon quantum dots (CQDs), nanoscale carbon particles, are emerging as multifunctional agents for plant protection. This review critically explores how the physicochemical properties of CQDs, especially those synthesized from green precursors, modulate plant responses to diverse stressors. CQDs help plants handle stress better by balancing reactive oxygen species (ROS), increasing the expression of defense-related genes, and improving cellular functions.

Advances in stem cell-based therapeutics for acute high-altitude illness: research progress and prospects.

Acute high-altitude illness (AHAI), triggered by hypobaric hypoxia following rapid ascent to high elevations, induces complex pathophysiological responses that may be life-threatening. Recent advances in regenerative medicine have highlighted the therapeutic potential of stem cells in mitigating hypoxia-induced damage. Among them, Mesenchymal stem cells (MSCs), the most extensively investigated, exert therapeutic efficacy through immunomodulation, attenuation of oxidative stress, and enhancement of tissue repair mechanisms.

GALNT18-Mediated O-Glycosylation Promotes Development of Nonalcoholic Steatohepatitis-Associated Hepatocellular Carcinoma Through Activation of EGR1/TGF-β1/Smad Signaling.

Nonalcoholic steatohepatitis-associated hepatocellular carcinoma (NASH-HCC) is an increasing global health issue. Research indicates that amyloid precursor protein (APP) might be a diagnostic marker and therapeutic target for HCC. However, the specific role of O-glycosylation of APP in NASH-HCC remains unclear.

Large-area graphene thermal emitter based on all-dielectric Fabry-Perot cavity in the near-infrared.

The exceptional saturation current density and ultrafast Joule heating modulation properties of graphene present significant potential for active thermal emission regulation. However, its practical implementation in near-infrared regimes has been constrained by inherent limitations in the low emissivity (absorptivity) and atmospheric oxidation susceptibility. In this study, we propose a large-area thermal emitter through the integration of graphene with an all-dielectric Fabry-Perot cavity.

Bioinformatic screen with clinical validation for the identification of novel stool based mRNA biomarkers for the detection of colorectal lesions including advanced adenoma.

Messenger RNA (mRNA) stool based biomarkers represent a promising approach for the diagnosis of colorectal cancer (CRC) and advanced adenoma (AA). But it is unclear which mRNA biomarkers have the most clinical utility. This study aims to partially fill this gap by performing an analysis which first ranks genes based on their expression profile in publicly available RNA-seq tissue datasets.

SIRPA, BTN3A1, and TDO2 in osteosarcoma: a prognostic triad with therapeutic implications from integrated genomic and pharmacogenomic data.

The limited understanding of the prognostic implications of immune checkpoint molecules in osteosarcoma (OS) poses significant challenges for improving patient outcomes. There is a gap in the identification of reliable biomarkers that can predict treatment response and prognosis in OS patients. This study focused on investigating the prognostic value of immune checkpoints, specifically BTN3A1, SIRPA, and TDO2, using data from the TARGET database and clinical follow-up data from our hospitals.

Real-world pharmacovigilance assessment of hepatotoxicity risk with HER2-Targeted drugs using FAERS database analysis.

Human epidermal growth factor receptor 2 (HER2)-targeted therapies, including monoclonal antibodies (mAbs), small-molecule tyrosine kinase inhibitors (TKIs), and antibody-drug conjugates (ADCs), have significantly improved clinical outcomes in HER2-positive cancers. However, hepatotoxicity remains a major concern with these therapies, and a comprehensive analysis of the hepatotoxicity signals across different HER2-targeted drugs is lacking. We aim to conduct a comprehensive comparative analysis of hepatotoxicity risk related to various HER2-targeted agents.

The global burden of lead exposure-related ischemic stroke: based on Bayesian age-period-cohort analysis.

Introduction: The global burden of lead exposure-related ischemic stroke poses a significant concern for public health, and this study aimed to comprehensively analyze the current situation, the change patterns, and forecast potential future trends associated with this critical issue.

Methods: The Global Burden of Disease (GBD) 2019 dataset was analyzed to assess the burden of lead exposure-related ischemic stroke. Comparison of the 2019 disease burden was conducted across age, gender, Sociodemographic Index (SDI) regions, and GBD regions.

Shifts in Fungal Communities and Potential Functions Under Masson Pine Forest-to-Tea Plantation Conversion in Subtropical China.

The land-use conversion of Masson pine forests to tea fields is extensively practiced across subtropical China, primarily driven by its economic benefit. However, the effects of this conversion on soil fungal communities and functional guilds are poorly understood. Herein, a field experiment was conducted in a Masson pine forest (F), a 5-year-old tea plantation without (FT-CK) fertilization or with (FT-N), and a 30-year-old tea plantation (FT-O) to assess the impact of Masson pine forest-to-tea conversion on soil fungal abundance, community structure, and functional guilds by using qPCR and high-throughput sequencing.

A bidirectional association between psychotic-like experiences and problematic Internet use: A large-sample longitudinal study among Chinese adolescents.

Purpose: Psychotic-like experiences (PLEs) are associated with problematic Internet use (PIU), but empirical studies on the causal relationships between both are lacking. This study aimed to investigate the bidirectional associations between PLEs and PIU through a large prospective study involving Chinese adolescents.

Methods: A total of 27,260 adolescents (M = 14.

Triple stem cell infusion alleviated graft-versus-host disease and improves outcomes in unmanipulated haploidentical hematopoietic stem cell transplantation.

Haploidentical hematopoietic stem cell transplantation (Haplo-HSCT) provides cure opportunity for patients requiring prompt allogeneic HSCT but failing to identify well-matched donor, but its outcomes are potentially impaired by increased transplant-related mortality (TRM). We performed haplo-HSCT using granulocyte colony-stimulating factor (G-CSF)-primed peripheral blood stem cells (PBSCs), umbilical cord mesenchymal stem cells (UC-MSCs) and third-party unrelated umbilical cord blood (UCB) stem cells. Modified "Beijing protocol" were performed in this study.

Decoding key aroma components of three cigar tobacco leaves based on molecular sensory science.

Cigar is a flavor-dependent cash crop. However, the key aroma compounds of tobacco leaves are less studied. In this study, we used molecular sensory science to explore the key aroma compounds of cigar tobacco leaves from three different origins in Yunnan, China.