Buyang huanwu decoction protects against cerebral ischemia-reperfusion injury by inhibiting complement and coagulation cascades: A proteomics and experimental study.

Objective: Proteomics Approach to Investigate the Mechanism of Buyang Huanwu Decoction(BYHWD) in Ameliorating Cerebral Ischemia-Reperfusion Injury through Inhibition of Complement and Coagulation Cascade Activation.

Methods: A Rat Model of Qi Deficiency and Blood Stasis Induced Cerebral Ischemia-Reperfusion Injury Was Established Using Exhaustive Swimming Combined with Middle Cerebral Artery Occlusion Model(MCAO). The therapeutic effects were evaluated through neurological scoring, TTC staining, and HE staining. Proteomics analysis revealed that Buyang Huanwu Decoction alleviates brain tissue damage by inhibiting the activation of complement and coagulation cascade pathways.

Results: In vivo and in vitro experiments demonstrated that Buyang Huanwu Decoction significantly improves neurological function, reduces infarct size and brain edema, inhibits the activation of complement and coagulation cascades, increases the release of anti-inflammatory factors, suppresses the release of pro-inflammatory factors, improves the blood-brain barrier, and inhibits abnormal synaptic cleavage.

Conclusion: In summary, BYHWD can improve brain tissue injury in cerebral ischemia-reperfusion injury with Qi deficiency and blood stasis syndrome. The mechanism may involve the inhibition of complement and coagulation cascade activation by BYHWD, thereby suppressing the inflammatory response, alleviating blood-brain barrier damage, and abnormal synaptic pruning, ultimately improving brain tissue injury after cerebral ischemia.