Optimal stimulation site and fiber tracts in subthalamic deep brain stimulation for Meige syndrome.

Background: Deep brain stimulation (DBS) of the subthalamic nucleus (STN) has emerged as an effective therapy for Meige syndrome (MS). However, the optimal stimulation site within STN and the most effective stimulation fiber tracts have not been investigated.

Methods: Based on the discovery cohort (n = 65), we first identified the optimal stimulation site within the STN using the sweet spot mapping method.

Unraveling Novel Genetic Determinants of Thiopurine Response Via TWAS.

Acute lymphoblastic leukemia (ALL) is the most common childhood cancer. Thiopurines such as 6-mercaptopurine (6MP) are essential in ALL maintenance therapy. However, dose-limiting toxicities can significantly disrupt treatment.

A dual-encoder U-net architecture with prior knowledge embedding for acoustic source mapping.

The deconvolution approach has become a standard method for high-resolution acoustic source mapping, but it suffers from a heavy computational burden. Deep learning-based methods have shown promising progress but often rely on single-type input features and ignore the position- and frequency-dependent variabilities of the point spread function (PSF), which leads to a decline in localization accuracy. This paper proposes a supervised learning framework based on dual-encoder U-net architecture to convert beamforming maps into a high-resolution map of true source strength distribution.

Hydrogen sulfide (HS) Alleviates Diabetic Myocardial Fibrosis by Suppressing Pyroptosis via Inhibiting DNMT3a-mediated Sestrin2 CpG Promoter Hypermethylation.

Background: Diabetic cardiomyopathy (DCM) represents an important concern associated with diabetes, inhibiting pyroptosis has shown promising results in alleviating DCM symptoms. The objective of this work is to investigate the role and underlying mechanism of hydrogen sulfide (HS) in the suppression of pyroptosis in the context of diabetic myocardial fibrosis (MF).

Methods: The effect of HS on pyroptosis was detected using CCK-8, ELISA, and flow cytometry.

[Analysis of early efficacy and safety of hip arthroscopy in patients with borderline developmental dysplasia of the hip].

Objective: To explore the early efficacy and safety of hip arthroscopy in the treatment of patients with borderline developmental dysplasia of the hip(BDDH).

Methods: A total of 111 patients diagnosed with BDDH from January 2020 to December 2022 were selected and divided into two groups according to the surgical method. Among them, 63 patients who underwent arthroscopy were assigned to the arthroscopy group, including 22 males and 41 females with an average age of (35.

[Expert consensus on prognostic evaluation of cochlear implantation in hereditary hearing loss].

Hearing loss is the most prevalent disabling disease. Cochlear implantation（CI） serves as the primary intervention for severe to profound hearing loss. This consensus systematically explores the value of genetic diagnosis in the pre-operative assessment and efficacy prognosis for CI.

Manganese mediates antiviral effects by driving an ATM-TBK1 phosphorylation signaling pathway.

We previously reported that manganese (Mn) enhances innate immune responses to viral infection by inducing phosphorylation of TANK-binding kinase 1 (TBK1) in an Ataxia-telangiectasia mutated (ATM)-dependent manner. However, the underlying mechanism by which how Mn induces TBK1 phosphorylation remained unclear. Here, we show that Mn dose-dependently induced TBK1 phosphorylation in the presence of ATM across multiple cell lines, as well as in primary human macrophages and T cells.

Therapeutic Potential of Compounds with High Affinity to BAG2 in Inhibiting Keloid Disease.

Purpose: Targeting the distinct genetic and protein expression profiles of keloids necessitates the identification of novel therapeutic targets. This study was aimed to elucidate the role of Bcl-2-associated athanogene 2 (BAG2) in keloid pathology and identify compounds with high-affinity to BAG2.

Patients And Methods: Cell migration, and cell proliferation assays, along with flow cytometry, were used to evaluate the effects of BAG2 on keloid fibroblasts (KFs) derived from tissue samples of patients with abdominal or chest keloids.

Exploring the landing speed of digital society PPP projects: A continuous-time event history analysis of 300 projects.

The Public-Private Partnership (PPP) model has become a viable alternative or supplement to traditional approaches in the development of a digital society. However, PPP projects in this domain often face significant landing (the launch of project implementation, typically marked by contract signing and the commencement of operational activities) challenges. Understanding the factors that influence the speed of project landing is thus of considerable practical importance.

Skin-Inspired Ultra-Linear Flexible Iontronic Pressure Sensors for Wearable Musculoskeletal Monitoring.

The growing prevalence of exercise-induced tibial stress fractures demands wearable sensors capable of monitoring dynamic musculoskeletal loads with medical-grade precision. While flexible pressure-sensing insoles show clinical potential, their development has been hindered by the intrinsic trade-off between high sensitivity and full-range linearity (R > 0.99 up to 1 MPa) in conventional designs.

Preservative effects of chlorogenic acid on myofibrillar proteins in golden pomfret: Integrating artificial neural network and molecular simulation.

Chlorogenic acid (CGA) was applied to maintain myofibrillar protein (MP) quality in refrigerated golden pomfret fillets. The 2 g/L CGA treatment outperformed the control group, showing higher Ca-ATPase activity (0.57 μmol/h/mg protein) and salt-soluble protein content (86.

Effectiveness of collagen nerve conduit combined with PRP for incomplete peripheral nerve injury: a retrospective case-control study.

Incomplete peripheral nerve injuries with structural pathological changes in the epineurium present treatment challenges, particularly when epineurotomy is required. This study investigates whether the combination of bovine collagen artificial nerve conduit (BCANC) and platelet-rich plasma (PRP) can mitigate the adverse effects of epineurotomy and promote more effective sensory and motor nerve recovery.A total of 222 patients (ages 16-75) with incomplete peripheral nerve injuries were enrolled.

MACML: Marrying attention and convolution-based meta-learning method for few-shot IoT intrusion detection.

The widespread deployment of Internet of Things (IoT) devices has made them prime targets for cyberattacks. Existing intrusion detection systems (IDSs) heavily rely on large-scale labeled datasets, which limits their effectiveness in detecting novel attacks under few-shot scenarios. To address this challenge, we propose a meta-learning-based intrusion detection method called MACML (Marrying Attention and Convolution-based Meta-Learning).

OTUB1 antagonizes TRIM21 to induce deubiquitination of SPHK1 and promote the progression of hepatocellular carcinoma.

SPHK1 is critical for maintaining cellular lipid balance. Aberrant expression of SPHK1 aggravates malignancy of tumor through multiple signaling pathways. Here, we report a novel regulatory mechanism in ubiquitination of SPHK1.

Silence as a Quiet Strategy: Understanding the Consequences of Workplace Ostracism Through the Lens of Sociometer Theory.

Existing research has predominantly framed defensive silence as an avoidance response to interpersonal mistreatments. Moving beyond this view, this study theorizes defensive silence as a proactive strategy for managing interpersonal relationships through the lens of sociometer theory. We posit that workplace ostracism will reduce employees' organization-based self-esteem (OBSE), which in turn increases their subsequent defensive silence to avert further damage to relationships.

Modular RCA-CRISPR/Cas12a amplification on a multi-volume SlipChip for ultrafast, single-copy quantification of circRNA and miRNA in ovarian cancer.

The aberrant expression of RNAs in ovarian cancer (OC) progression highlights their potential as clinical biomarkers. However, rapid and accurate quantification of these RNAs in biosamples remains a significant challenge. In this study, we develop a modular isothermal rolling circle amplification (RCA)-activated Cas12a loop-enhanced (MIRACLE) amplification method for circRNA and miRNA quantification without the need of reverse transcription.

D-S-Net: an efficient dual-stage strategy for high-precision segmentation of gross tumor volumes in lung cancer CT images.

Background: Accurate delineation of Gross Tumor Volume (GTV) in lung cancer is critical for effective radiotherapy and surgical planning. However, segmentation of GTV in high-resolution CT images remains challenging, particularly when tumors are small or have indistinct boundaries.

Methods: We propose D-S-Net, a novel dual-stage strategy to enhance both the accuracy and efficiency of lung cancer GTV segmentation.

Ultrasound Localization Microscopy for the Assessment of Microvascular Abnormalities in Transplant Renal Artery Stenosis.

Objective: To evaluate the clinical validity of ultrasound localization microscopy (ULM)-based microvasculomics for quantitatively detecting microcirculatory dysfunction in transplant renal artery stenosis (TRAS).

Methods: In this study, 54 renal transplant recipients underwent digital subtraction angiography (DSA) and ultrasound examination. They were divided into TRAS (n = 15) and control (n = 39) groups.

Aldosterone and Cardiovascular Risk Across the Lifespan.

Aldosterone excess, particularly in the context of primary aldosteronism, is associated with adverse cardiovascular outcomes. Historically considered a condition of resistant hypertension with hypokalaemia, patients with primary aldosteronism often experienced prolonged diagnostic delay with significant end-organ damage involving the renal, cardiovascular, and central nervous systems at diagnosis. Emerging research has revealed a wide spectrum of renin-independent aldosteronism, ranging from subclinical disease with normal or mildly elevated BP to overt disease marked by resistant hypertension and cardiovascular complications.

A Stepwise Management Strategy for Treatment of Iatrogenic Upper Eyelid Retraction in Eastern Asian Eyelids.

Background: Incidence of iatrogenic upper eyelid retraction (UER) caused by blepharoptosis overcorrection and improper blepharoplasty is increasing fast among Eastern Asians. The aim of this study is to present our experience using techniques of pretarsal cicatrix release, recession of levator-Müller's muscle complex, and lengthening of levator-Müller's muscle complex to correct mild to severe UER, and evaluate their anesthetic surgical outcomes.

Methods: Patients with UER who underwent surgical repair using the stepwise management strategy from December 2018 to June 2022 were retrospectively reviewed.

Single brand tire wear particles promote toxin-producing of an invasive cyanobacterium.

Tire wear particles (TWPs), as newly emerging pollutants, frequently co-occur with potentially toxic cyanobacteria in eutrophic waters. However, it is unknown how these new pollutants affect ecology of mass bloom-forming cyanobacteria. Here, we compared single brand and mixed brand TWPs how to affect the invasive and toxic cyanobacterium Raphidiopsis raciborskii.

Efficient induction of tetraploids via adventitious bud regeneration and subsequent phenotypic variation in Acacia melanoxylon.

BACKGROUND ACACIA MELANOXYLON: is an important species for establishing pulpwood plantations due to its high application value in engineered wood products. However, the lack of a well-established in vitro regeneration system has severely constrained its industrial-scale propagation and the induction of tetraploids. RESULTS: In this study, using the superior A.

Development of Thymic Organoids Heterotopically to Educate and Induce T Lymphocytes.

Background: Vascularized composite allotransplantation (VCA) is a potential treatment for extensive injuries that replaces defects like-with-like, however allografts are immune-rejectable.

Methods: This study developed in vitro thymic organoids and examined whether donor-derived HSCs could be educated in vivo into T lymphocytes via central tolerance. TECs, TMCs, and HSCs from C57BL/7 (CD45.