Extramedullary relapse of acute lymphoblastic leukemia treated with a CAR-T cell therapy bridge to unrelated cord blood transplantation: a case report and review of the literature.

Extramedullary relapse of acute lymphoblastic leukemia (ALL) is usually associated with poor prognosis. Chimeric antigen receptor T cell (CAR-T cell) therapy followed by allogeneic hematopoietic stem cell transplantation is beneficial for relapsed/refractory (r/r) B cell acute lymphoblastic leukemia (B-ALL). Here, we report a B-ALL patient with extramedullary relapse involving several organs, including multiple lymph nodes and the breast, kidney, uterus and pancreas.

The Construction of a Mutant Library of Linseed and the Molecular Mechanism of High Oleic Acid Mutants from a Multi-Omics Perspective.

Flax ( L.) is a globally important oilseed crop, valued for its edible and industrial uses. Flax seeds are rich in unsaturated fatty acids.

2.5D Deep Learning and Machine Learning for Discriminative DLBCL and IDC with Radiomics on PET/CT.

We aimed to establish non-invasive diagnostic models comparable to pathology testing and explore reliable digital imaging biomarkers to classify diffuse large B-cell lymphoma (DLBCL) and invasive ductal carcinoma (IDC). Our study enrolled 386 breast nodules from 279 patients with DLBCL and IDC, which were pathologically confirmed and underwent F-fluorodeoxyglucose (F-FDG) positron emission tomography/computed tomography (PET/CT) examination. Patients from two centers were separated into internal and external cohorts.

Biomembrane-inspired lipid nanoparticles enhance CRISPR-Cas9 editing for hemophilia A.

Lipid nanoparticles (LNPs) have substantially advanced RNA-based therapies; however, their use for CRISPR-Cas9 remains limited by sub-optimal endosomal escape, innate immune activation, transient nuclease expression, and restricted tissue specificity. Here, we engineered biomembrane-inspired LNPs containing sphingomyelin and C18-galactosyl ceramide (C18-GalCer) to improve liver-targeted CRISPR delivery. The optimized formulation increased in vivo editing efficiency 2.

Patterns of Serum Prolactin Elevation Associated with Nine Second-Generation Antipsychotics in a Large Cohort of Patients with Schizophrenia.

Background: Prolactin elevation associated with antipsychotic use significantly affects medication adherence and long-term treatment outcomes in patients with schizophrenia. The currently available data are insufficient to guide the monitoring and management of elevated prolactin levels in patients on antipsychotic medications. This study aimed to explore the patterns of prolactin level elevation associated with nine second-generation antipsychotics (SGAs) in a real-world setting and compare the associated risks.

Good performance of monoclonal antibody-based stool H. pylori antigen tests in acute nonvariceal upper gastrointestinal bleeding patients.

Background: Acute nonvariceal upper gastrointestinal bleeding (ANVUGIB) is a common clinical emergency disease with high morbidity and mortality. H. pylori (HP) testing during hospitalization increases the eradication rate of HP and improves the prognosis of patients.

Asparaginase enhances CAR-T cell antitumor immunity by asparagine metabolic reprogramming and central memory induction in ALL.

High levels of asparagine synthetase (ASNS) in acute lymphoblastic leukemia (ALL) lead to immunotherapy resistance. Our study showed that ASNS overexpression (OE) in NALM6-GL cancer cells attenuated chimeric antigen receptor (CAR)-T cell-mediated cancer cell lysis. Asparaginase (ASPG) is an approved drug that breaks down circulating asparagine in leukemia cells, thereby depriving cancer cells of asparagine and inhibiting cancer growth.

The Application of IoT-Based Information Collection Platform in Intelligent Management of Cardiovascular Diseases.

Effective monitoring of the daily lives of patients with cardiovascular chronic diseases through smart healthcare technologies can help improve their quality of life, while also reducing the mortality and readmission rates associated with these diseases. Based on this, the present study aims to utilize the Internet of Things (IoT)-enabled wearable devices and machine learning techniques to collect health data from cardiovascular patients in their home environments. The goal is to enhance the accuracy and comprehensiveness of data collection and to construct a standardized health dataset.

Effectiveness of Transitional Care Intervention on Health Outcomes in Patients with Pci: A Systematic Review and Meta-Analysis.

Unlabelled: Transitional care interventions have been proposed to improve outcomes in chronic patients transitioning from hospital to home. This systematic review and meta-analysis aimed to evaluate the impact of transitional care interventions on readmission rates, quality of life, anxiety, and depression in post-PCI patients.

Methods: A comprehensive search was conducted across PubMed, Embase, Web of Science, CINAHL, and Cochrane Library for studies published up to November 26, 2024.

Chronic stress in mice: how gut bacteria influence gene activity in key brain neurons.

Major depressive disorder (MDD) is a serious mental disorder. Increasing evidence suggests that changes of gut microbiota are involved in pathogenesis of depression, yet the underlying mechanisms remains unknown. Here, chronic unpredictable mild stress (CUMS) mice model was constructed to mimic depression.

Association study of ADORA2A gene polymorphisms with adverse drug reactions to valproic acid and oxcarbazepine in the treatment of children with epilepsy.

Objective: Adenosine, a purine nucleotide, is implicated in various brain disorders. Our previous study identified a correlation between ADORA2A variants and epilepsy susceptibility. However, it remains unclear whether ADORA2A genetic polymorphisms affect the outcomes of antiseizure medication (ASM) therapy.

Exploring wastewater treatment plant operation and performance using k-means and Gaussian mixture clustering.

Efficient wastewater treatment is critical to protecting the environment and minimizing human impacts on natural watercourses. Optimizing wastewater treatment to meet compliance objectives while avoiding excessive energy use can be challenging, particularly for plants relying on exclusively manual operation. Even when large volumes of data are available, it can be challenging to derive useful insight from this data to better understand plant behaviours.

New Diagnostic and Therapeutic Targets for Spinal Cord Injury: GRN Gene.

Spinal cord injury (SCI) is a severe disabling disease due to the poor self-healing of the central nervous system. Studies showed that many N6-methyladenosine (m6A) RNA methylation profiles are hypomethylated after SCI, which are related to neural regeneration and different m6A marker genes. In addition, immune cell infiltration may significantly affect the development and progression of SCI.

A potentially pathogenic KDM5C variant in X-linked high myopia.

High myopia is a multifactorial ocular disease that demonstrates high genetic susceptibility and significant family aggregation. It has multiple inheritance patterns, including X-linked inheritance; however, few genetic variants associated with X-linked high myopia have been documented. Using a whole exome sequencing approach, we have identified a novel missense variant (c.

Cost-effectiveness analysis of first-line treatment for advanced soft tissue sarcomas in China.

Objectives: To evaluate the cost-effectiveness of four first-line treatment strategies-anlotinib, pazopanib, regorafenib, and conventional chemotherapy-for patients with advanced soft tissue sarcomas (STSs) in China.

Methods: A partitioned survival model was developed to simulate costs and quality-adjusted life years (QALYs) over a lifetime horizon from the Chinese healthcare system perspective. Relative treatment efficacies were derived from a network meta-analysis (NMA).

Texture features-guided image reconstruction kernel method forF-FDG delayed PET imaging.

Positron emission tomography (PET) is a crucial imaging technology that is widely used for cancer staging and treatment response assessment. Delayed PET imaging (or dual-time-point imaging) involves a second PET acquisition step approximately 33 min after the initial scan in this study, providing more dynamic biological information, improving lesion detectability, and allowing patients to rest during the scan. However, with increasing time, the number of photons in the second scan decreases, which raises the difficulty of the second PET image reconstruction process.

Relationship Between Social Support, Symptom Burden, Dyspnea, Perceived Stress, Perceived Stigma, Coping Styles, and Psychological Distress in Patients with Stable COPD: A Structural Equation Model.

Background: Psychological distress is prevalent in patients with stable chronic obstructive pulmonary disease (COPD) and may contribute to disease progression. However, the interplay among its influencing factors remains unclear. This study aimed to explore how social support, symptom burden, dyspnea, perceived stress, perceived stigma, and coping styles impact psychological distress in stable COPD using a structural equation model (SEM).

Single-cell analysis of peripheral blood and pleural effusion reveals functional diversity of γδ T cells in tuberculosis infection.

Introduction: Tuberculosis is a contagious airborne disease caused by the Mycobacterium tuberculosis infection. γδ T cells are closely associated with TB infection; however, the specific role of γδ T cells in the immune response to TB remains unclear, as does the differentiation and mechanism of γδ T cell subsets in TB patients.

Methods: We analyzed the characteristics of γδ T subsets in the peripheral blood (Peripheral Blood Mononuclear Cells,PBMC) and pleural effusions (Tuberculous pleural effusion,TPE) and pleural effusions of TB patients using single-cell sequencing to explore the distribution and characteristics of different γδ T subpopulations.

Wet-electrospun porous freeform scaffold enhances colonisation of cells.

Osteoarthritis is a degenerative disease characterized by the progressive deterioration of articular cartilage. Electrospun scaffolds have shown promise in the regeneration of degraded areas due to their highly interconnected and extracellular matrix-mimicking structures. However, current electrospun scaffold-based therapies are limited by the constraints of 2D cell culture.

RNA-seq analysis identifies key genes and signaling pathways involved in androgen promotion of sebaceous gland proliferation in Hetian sheep.

Sebaceous glands (SGs), essential elements of the skin barrier in sheep, are tightly regulated by the androgen signaling pathway. This study investigated how androgens influence SGs hyperplasia in sheep by combining morphological assessments with transcriptomic analysis. Histological examination revealed a significant increase (P < 0.

Effect of foliar spray selenium on antioxidant defense system, yields, fatty acid composition, and mineral concentrations in flax ( L.).

Introduction: Flax is an important multipurpose oil crop with high-quality seed, oil, and fibre.

Methods: This study aims to investigate the effect of selenium (Se) fertilization on the flax antioxidant defense system, yields, fatty acid composition, and mineral concentrations. A field experiment was conducted in Gansu, Northwest China in 2022, 2023, and 2024, by foliar application of Se at early budding, early flowering, and early seed-filling using a low-pressure hand sprayer.

Single-cell transcriptomics reveals Sox6 positive interneurons enriched in the prefrontal cortex of female mice vulnerable to chronic social stress.

Major depressive disorder (MDD) is a prevalent mental illness that significantly impacts global health, with women showing twice the prevalence of men. This study employed the chronic social defeat stress (CSDS) model in female mice to investigate cellular and molecular changes in the prefrontal cortex (PFC) associated with depressive-like behaviors. Using single-nucleus RNA sequencing (snRNA-Seq), we examined transcriptomic alterations across various cell types in the PFC.

A dorsal subiculum-medial mammillary body pathway for spatial memory.

The dorsal subiculum (dSub) and medial mammillary body (MM) are essential components of the extended hippocampal system, heavily implicated in spatial memory processes. However, the exact function of the prominent dSub-to-MM projection has remained ambiguous. Here, integrating c-fos mapping, fiber photometry, optogenetic manipulations and behavioral paradigms, we found that the monosynaptic connection between dSub and MM, rather than collaterals to other regions, is not only involved in spatial memory retrieval but also highly vulnerable to perturbations on activity level in both directions.

Myopia and high myopia trends in Chinese children and adolescents over 25 years: a nationwide study with projections to 2050.

Background: The global rise in myopia, particularly in Asia, presents significant public health challenges. Analyzing trends and forecasting impacts are critical for developing strategies to mitigate this burden.

Methods: We conducted the largest study to date on myopia and high myopia prevalence in Chinese children and adolescents aged 7-18 years, analyzing data from 5,095,256 individuals across 119 studies from 1998 to 2022.

The molecular response of Neoseiulus bicaudus to cold acclimation.

Neoseiulus bicaudus is a beneficial predatory mite used for the control of spider mites. Temperature is a crucial factor that influences the distribution, growth, and development of N. bicaudus.