Association of the PWWP-domain protein HUA2 and the H3K36 methylation in flowering time control.

Trimethylation of histone H3 at lys36 (H3K36me3) promotes gene transcription and governs plant development and plant responses to environmental cues. Yet, how H3K36me3 is translated into specific downstream events remains largely uninvestigated. Here, we report that the Arabidopsis PWWP-domain protein HUA2 binds methyl-H3K36 in a PWWP motif-dependent manner.

Ethanol-mediated freeze-drying enables robust bacterial cellulose aerogels for enhanced drug loading and hemostasis dressing.

This work reports an ethanol-mediated freeze-drying (EMFD) strategy that enables the scalable production of high-performance bacterial cellulose aerogels (BCAs), effectively addressing key limitations of conventional methods such as supercritical drying and standard freeze-drying, including fragility, low mechanical strength, and high cost. Specifically, by replacing water in bacterial cellulose hydrogels (BCHs) with ethanol-water solution (EWs) prior to freeze-drying, the process limits ice crystal formation and reduces capillary forces and adhesion, thereby preserving structural integrity and enhancing mechanical properties. The effects of EWs concentration on BCA morphology, volume shrinkage, mechanical strength, and pore structure were systematically investigated.

-OH/NH-promoted reductive deoxygenation of α,β-unsaturated ketones with HBpin.

A solvent- and catalyst-free protocol for the selective reductive deoxygenation of α,β-unsaturated ketones with pinacolborane (HBpin) has been developed. -OH/NH groups efficiently direct the transformation, affording 2-allylphenols and 2-allylanilines under mild conditions with excellent chemo- and regioselectivity. Mechanistic studies indicate a boron-assisted hydride transfer and carbocation pathway.

BUB1B promotes homologous recombination‑mediated DNA damage repair in breast cancer cells through the PI3K/AKT signaling pathway.

Radioresistance is a major obstacle to effective radiotherapy in breast cancer. BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B) is involved in numerous biological processes associated with cancer; however, its specific role in mediating radioresistance in breast cancer remains poorly characterized. The present study first evaluated its expression profile and association with patient prognosis through bioinformatics analysis.

Development of a multi-feature predictive model for risk stratification in stage IB-IIA non-small cell lung cancer: a multicenter analysis.

Objective: This study aimed to develop a comprehensive risk stratification model for stage IB-IIA non-small cell lung cancer (NSCLC) by integrating clinicopathological data with pre-treatment CT imaging.

Methods: This retrospective study included three independent cohorts of patients with stage IB-IIA NSCLC for model development and validation (Training: n = 370; Internal validation: n = 120; External validation: n = 70). Disease-free survival (DFS) was the primary endpoint.

Specific Flavor Substances of Liqueur Koji Fermented Foxtail Millet Beverage: As Indicators of Flavor Characteristics and Antioxidant Capacity.

Liqueur koji-fermented foxtail millet beverages offer distinctive flavors and health benefits, but the interrelationships among flavor compounds, sensory properties, and antioxidant activity remain unelucidated. This study systematically mapped dynamic changes across a standardized 72 h fermentation using chromatographic, electronic sensory approaches, and antioxidant assays. Key results revealed glucose, lactic acid, and succinic acid as primary taste-active indicators through HPLC.

Serum Pepsinogen as a Biomarker of Gastrointestinal Stromal Tumors (GIST) in Stomach.

Background: Gastric GISTs (GG) are significant mesenchymal tumors. No biomarker has been identified for GG detection. We first observed mucosal atrophy surrounding GG tumors, leading to the hypothesis that localized atrophy may alter serum pepsinogen (PG) levels.

[Research progress on the diagnosis of ectodermal dysplasia and early oral prosthodontic treatment].

Ectodermal dysplasia is a group of hereditary diseases characterized by developmental defects of ectodermal structures. Its oral manifestations mainly center on congenital missing teeth, abnormal tooth morphology, and maxillofacial bone developmental disorders, which seriously affect the masticatory function, maxillofacial development, and mental health of affected children. In this article, the multidimensional diagnostic strategy system for children with ectodermal dysplasia and the related progress of early oral prosthodontic treatment methods were systematically reviewed to provide references for clinicians in the diagnosis and treatment of children with ectodermal dysplasia.

[Expert consensus on local anesthesia application in pediatric dental therapies].

Dental treatments for children and adolescents have unique clinical characteristics that differ from dental care for adults in terms of children's physiology, psychology, and behavior. These differences impose specific requirements on the application of local anesthesia in pediatric dental procedures. This article presents expert consensus on the principles of local anesthesia techniques in pediatric dental therapies, including the use of common anesthetic drugs and dosage control, safety and efficacy evaluation, and prevention and management of complications.

Effects of on growth performance, hepatopancreatic transcriptome and resistance to ammonia-nitrogen stress of pacific white shrimp ().

Astaxanthin is widely used in aquatic animal feed and has beneficial effects on aquatic animals. is one of the most important sources of natural astaxanthin. In this 8-week feeding experiment, the impacts of gradient additions of on the growth and physiological performance of before and after acute ammonia nitrogen stress were investigated.

Drug-coated balloon angioplasty versus conventional angioplasty for symptomatic non-acute intracranial artery occlusion.

Background And Objective: Non-acute intracranial artery occlusion (ICAO) is an important cause of recurrent ischemic stroke despite aggressive medical treatment. Conventional angioplasty has high incidences of perioperative complications and restenosis in treating these patients. Drug-coated balloon (DCB) treatment has shown superiority in preventing restenosis.

Genomic Characterization of Lung Cancer in Never-Smokers Using Deep Learning.

Despite promising results in using deep learning to infer genetic features from histological whole-slide images (WSIs), no prior studies have specifically applied these methods to lung adenocarcinomas from subjects who have never smoked tobacco (NS-LUAD) - a molecularly and histologically distinct subset of lung cancer. Existing models have focused on LUAD from predominantly smoker populations, with limited molecular scope and variable performance. Here, we propose a customized deep convolutional neural network based on ResNet50 architecture, optimized for multilabel classification for NS-LUAD, enabling simultaneous prediction of 16 molecular alterations from a single H&E-stained WSI.

Naringenin as a neurotherapeutic agent in Alzheimer's disease: epigenetic signatures, gut microbiota alterations, and molecular neuroprotection.

Alzheimer's disease (AD) remains a major neurodegenerative disorder characterized by progressive cognitive decline, amyloid- (Aβ) aggregation, tau pathology, oxidative stress, and chronic neuroinflammation. In recent years, the dietary flavonoid naringenin, abundant in citrus fruits, has gained attention as a multi-target neuroprotective agent with potential application in AD therapy. Preclinical studies demonstrate that naringenin exhibits robust antioxidant activity, notably through activation of the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathway, which reduces ROS and preserves mitochondrial integrity.

Attenuated Salmonella typhimurium L forms combined with lentivirus shRNA-HOTAIR effectively inhibit tumor growth and metastasis in murine epithelial ovarian cancer.

Epithelial ovarian cancer (EOC) is a common gynecological malignant tumor, with a high mortality rate. HOX antisense intergenic RNA (HOTAIR) in lncRNAs is involved in various tumor epithelial-mesenchymal transition (EMT) processes. For seeking better treatment strategies, we studied the effects of attenuated Salmonella typhimurium (ST) L forms combined with lentivirus shRNA-HOTAIR on in vivo tumorigenicity and metastasis of murine EOC cells, and the related anti-tumor mechanisms.

Transcription Factor OsNF-YC12 Positively Regulates Resistance in Rice through Activation.

Globally, rice () is one of the most important food crops, but its production is threatened by root-knot nematodes (). Although NF-YC transcription factors play important roles in plant growth and development, their involvement in disease resistance has been poorly characterized. In this study, transcriptome analysis revealed that transcription factor OsNF-YC12 was upregulated upon treatment with nematode PAMP (Ascr#18).

Spatial and single-cell transcriptomics reveal keratinocytes as key players in vulvar lichen sclerosus pathogenesis.

Vulvar diseases are a neglected area of women's health, profoundly affecting patients' quality of life. Lichen sclerosus (LS) is a chronic inflammatory vulvar skin disorder leading to severe itching, pain, scarring, and an increased risk of malignancy. Despite this burden, the molecular pathogenesis of vulvar LS (VLS) is not well understood, limiting treatment options.

A novel technology based on aeration-coupled molecular oxygen activation for in-situ sustainable groundwater remediation: Mechanisms and feasibility assessment.

In-situ chemical oxidation (ISCO) based on Fe(II)-activated O is a promising approach for groundwater remediation; however, its efficiency is often limited by the low oxygen content in subsurface environments and insufficient generation of reactive oxygen species (ROS). In this study, a novel ISCO technology was developed by integrating a tripolyphosphate (TPP)-enhanced Fe(II)/O advanced oxidation system with aeration to promote ROS generation and enhance remediation performance. Two-dimensional sand tank experiments were conducted to evaluate the effectiveness of this approach in remediating p-Nitrophenol (PNP) contaminated groundwater.

Expert consensus on the treatment of oral diseases in pregnant women and infants.

With the growing emphasis on maternal and child oral health, the significance of managing oral health across preconception, pregnancy, and infancy stages has become increasingly apparent. Oral health challenges extend beyond affecting maternal well-being, exerting profound influences on fetal and neonatal oral development as well as immune system maturation. This expert consensus paper, developed using a modified Delphi method, reviews current research and provides recommendations on maternal and child oral health management.

Whole genome resequencing reveals genetic markers for plumage colour in Jingyuan Chicken.

In order to investigate the basic genetic structure of plumage colour in Jingyuan chicken and to explore the genetic markers for plumage colour development, the present study was carried out to investigate the candidate key SNPs and candidate genes regulating black, linen and white plumage and plumage traits of Jingyuan chicken by using selection signal analysis and genome-wide association analysis. Selection signal analyses showed that including 30, 40 and 18 overlapping genes were associated with black, linen and white plumage colours in Jingyuan chicken. Meanwhile, integrative genomic analyses identified BCAT1, LMO3, and PIK3C2G as primary candidates for black plumage, and IL1RAPL1 for white plumage, with all genes showing convergent support across multiple complementary approaches.

Pemafibrate treatment produces antidepressant-like effects in CUMS and CRS models through activation of hippocampal PPARa and BDNF signaling.

Background: It is well established that peroxisome proliferator-activated receptor α (PPARα) plays a crucial role in the pathogenesis of depression. Several PPARα agonists, including WY14643, fenofibrate, and gemfibrozil, have been reported to produce antidepressant-like effects in mouse models through PPARα-mediated enhancement of hippocampal brain-derived neurotrophic factor (BDNF) signaling and neurogenesis. Pemafibrate is a novel and highly selective modulator of PPARα, and therefore, we hypothesised that it might also exhibit antidepressant-like efficacy.

RDPNet: A Multi-Scale Residual Dilated Pyramid Network with Entropy-Based Feature Fusion for Epileptic EEG Classification.

Epilepsy is a prevalent neurological disorder affecting approximately 50 million individuals worldwide. Electroencephalogram (EEG) signals play a vital role in the diagnosis and analysis of epileptic seizures. However, traditional machine learning techniques often rely on handcrafted features, limiting their robustness and generalizability across diverse EEG acquisition settings, seizure types, and patients.

Design and Characterization of Curcumin-Modified Polyurethane Material with Good Mechanical, Shape-Memory, pH-Responsive, and Biocompatible Properties.

In the context of critical challenges in curcumin-modified polyurethane synthesis-including limited curcumin bioavailability and suboptimal biodegradability/biocompatibility-a novel polyurethane material (Cur-PU) with good mechanical, shape memory, pH-responsive, and biocompatibility was synthesized via a one-pot, two-step synthetic protocol in which HO-PCL-OH served as the soft segment and curcumin was employed as the chain extender. The experimental results demonstrate that with the increase in Cur units, the crystallinity of the Cur-PU material decreases from 32.6% to 5.

Identification of Rhododendron mariae extraction as a new attachment inhibitor against dengue virus by targeting the envelope protein domain III.

Introduction: Dengue virus (DENV), a mosquito-borne flavivirus, leads to over 390 million annual infections worldwide, and there are no approved antivirals so far. Rhododendron mariae (RM), a traditional Chinese herb abundant in flavonoids and triterpenoids, is used to treat respiratory disorders, yet its antiviral potential has been little explored. This study sought to assess the activity and mechanism of RM-1, an extract from RM, against DENV.

Comprehensive Transcriptomic and m6A Epitranscriptomic Analysis Reveals Colchicine-Induced Kidney Toxicity via DNA Damage and Autophagy in HK2 Cells.

Colchicine is commonly prescribed for inflammation and gout, but its nephrotoxicity and underlying mechanisms remain incompletely understood. The objective of this research was to clarify the association between m6A methylation modifications and nephrotoxicity caused by colchicine. A significant decrease in HK2 cell viability was observed following colchicine treatment, and mRNA sequencing (mRNA-seq) revealed the differential expression of genes associated with DNA damage and autophagy.

Integrated Proteomic and Transcriptomic Analysis Reveals the Mechanism of Selenium-Mediated Cell Wall Polysaccharide in Rice ( L.) Cadmium Detoxification.

Cadmium (Cd) toxicity destroys plant cells and affects plant growth and development. Due to its unique metallic properties, selenium (Se) has been shown to be effective in antioxidants, cellular immunity, and heavy metal detoxification. When Se and Cd are present together in plants, they antagonize.