BUB1B promotes homologous recombination‑mediated DNA damage repair in breast cancer cells through the PI3K/AKT signaling pathway.

Radioresistance is a major obstacle to effective radiotherapy in breast cancer. BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B) is involved in numerous biological processes associated with cancer; however, its specific role in mediating radioresistance in breast cancer remains poorly characterized. The present study first evaluated its expression profile and association with patient prognosis through bioinformatics analysis.

Targeted deprivation of STAT6 sensitizes acute lymphoblastic leukemia cells to cytarabine in vivo and in vitro: clinical implications.

Chemotherapy is the leading treatment for acute lymphoblastic leukemia (ALL). However, many ALL patients eventually develop relapses, the treatment of which remains a major challenge due to their chemoresistance phenotype. As a step towards this end, we here uncovered that relapsed ALL specimens exhibit a significantly lower expression of STAT6 but not of other STATs, when compared with their paired diagnosis specimens.

Predictive Value of the Estimation of Physiologic Ability and Surgical Stress for Postoperative Recurrent Laryngeal Nerve Injury in Patients With Type 2 Diabetes Mellitus Complicated by Thyroid Cancer: A Retrospective Cohort Study.

In the postoperative management of patients with type 2 diabetes mellitus (T2DM) and thyroid cancer, recurrent laryngeal nerve (RLN) injury is a significant complication. Due to existing metabolic abnormalities, surgical stress may further impair the recovery of physiological functions in these patients. This study aimed to investigate the predictive value of the Estimation of Physiologic Ability and Surgical Stress (E-PASS) system for postoperative RLN injury in patients with T2DM complicated by thyroid cancer.

Integrating eDNA and morphology reveals arsenic-associated assembly processes of benthic invertebrates in coral reef ecosystems.

Understanding how climate change and human activities affect the biodiversity and community assembly of benthic invertebrates is essential for monitoring and conserving coral reef ecosystems under accelerating environmental stress. Environmental DNA (eDNA) metabarcoding provides a rapid, standardized tool for biodiversity assessment, yet limitations such as primer bias and incomplete reference databases prevent it from fully replacing traditional morphological identification. Here, we proposed an integrated framework that combines morphological identification with eDNA metabarcoding to enhance the accuracy and scalability of benthic invertebrate diversity assessments in coral reef ecosystems.

Long-Acting and Stapled GLP-1R/GIPR/GCGR Triple Agonist for the Treatment of Obesity and Atherosclerosis.

Unimolecular multireceptor coagonists have emerged as a promising approach in the development of next-generation GLP-1 therapeutics. Herein, we describe the development of a long-acting and stapled GLP-1R/GIPR/GCGR triple agonist that exhibits balanced bioactivities comparable with those of their native ligands along with improved pharmacokinetic parameters. A robust and straightforward solid-phase Ugi macrocyclization strategy enables the facile synthesis of targeted peptides with a side-chain protractor attached on the exocyclic lactam bridge.

Klotho levels in chronic overwork stress: An animal model study.

Sudden death from overwork, or "karoshi," is poorly understood, particularly regarding its underlying pathogenesis. Forensic studies have shown myocardial interstitial fibrosis in patients with a history of prolonged overwork, but the mechanisms connecting overwork to cardiovascular disorders remain unclear. This study investigates Klotho expression in the heart and kidney of an overworked rat model.

Genetic redundancy of catechol 1,2-dioxygenase conferred the ability of Rhodococcus opacus PD630 for efficient catecholic compounds degradation.

Lignin, a bountiful natural resource, presents challenges in degradation and conversion due to its complex structure. Microorganisms have evolved a "biofunnel" pathway in nature that offers novel insights into lignin valorization. Among the key intermediates in lignin metabolism is catechol, predominantly metabolized by catechol 1,2-dioxygenase (CatA) in these organisms.

Automatic dental age estimation in adolescents via oral panoramic imaging.

Object: In forensic dentistry, dental age estimation assists experts in determining the age of victims or suspects, which is vital for legal responsibility and sentencing. The traditional Demirjian method assesses the development of seven mandibular teeth in pediatric dentistry, but it is time-consuming and relies heavily on subjective judgment.

Methods: This study constructed a largescale panoramic dental image dataset and applied various convolutional neural network (CNN) models for automated age estimation.

An analysis of gene expression profiles through machine learning uncovers the new diagnostic signature for diabetic foot ulcers.

Purpose: Diabetic foot ulcers (DFUs), a serious diabetes complication, greatly increase disability and mortality, underscoring the need for effective diagnostic markers.

Methods: We used GSE199939 and GSE134431 datasets from the Gene Expression Omnibus (GEO) database, removed batch effects, and identified differentially expressed genes (DEGs). The weighted gene co-expression network analysis (WGCNA) was used to identify co-expression modules, followed by the integration of the protein-protein interaction (PPI) network to screen key genes, which were further optimized using LASSO regression.

Preliminary Study on Carboxymethyl Chitosan-Based Carbon Dots for Tracing and Promoting Osteogenic Differentiation.

Maxillofacial bone defects, a common challenge in oral and maxillofacial surgery, affect over 2 million patients globally each year due to tumor resection, trauma, or infection. Beyond their role in mastication and speech, the structural integrity of jaw bones is critical for facial aesthetics. Current clinical treatments rely on autologous bone grafts, which are limited by donor site morbidity, or allografts with immune rejection risks.

Peripheral blood immune landscape and NXPE3 as a novel biomarker for hypertensive intracerebral hemorrhage risk prediction and targeted therapy.

We employed bulk RNA-seq and scRNA-seq techniques to analyze the immune dysregulation in patients with intracerebral hemorrhage (ICH). The study revealed that excessive inflammatory responses, neutrophil activation, and T-cell dysfunction are the main characteristics of ICH. A multi-machine learning framework was utilized to construct a predictive model for ICH risk in hypertensive patients.

Structure from motion-convolutional neural network model (SfM-CNN) achieved accurate portable Chinese dietary chemical composition estimation for dietary recall.

Accurately estimating the chemical composition of dietary intake is essential for health and nutrition management, especially in regions with complex culinary diversity like China. This study introduces a novel AI-driven solution using a Structure from Motion-Convolutional Neural Network (SfM-CNN) model to automate chemical composition analysis of Chinese food. By integrating advanced 3D reconstruction techniques with deep learning, specifically the Scale-Invariant Feature Transform (SIFT) algorithm, we achieved superior feature extraction and food volume estimation with less than 4 % error.

The complete mitogenome of (Liu & Hu 1962) (Anura: Rhacophoridae) from the Karst areas of southwestern China.

This study reports and characterizes the complete mitogenome of . The mitogenome was 15,780 bp in length and contained 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes, with 29.35% A, 25.

Impact of Implementation Interruptions of 1,7-Malaria Reactive Community-Based Testing and Response Approach on Malaria Control Efforts - Southern Tanzania.

Introduction: Surveys from the China-Tanzania Malaria Control Project demonstrated that the 1,7-malaria Reactive Community-Based Testing and Response (1,7-mRCTR) approach significantly reduced malaria incidence rates. However, implementation was disrupted by security concerns, infectious disease outbreaks, and supply shortages. This study evaluates how these interruptions affected intervention effectiveness to inform future malaria control strategies.

OAT1/3 regulate the absorption of CP-25 in the rat synovium.

Paeoniflorin-6'-O-benzene sulfonate (CP-25) is a new ester derivative formed by esterification of paeoniflorin. We found that CP-25 inhibits the abnormal proliferation and migration of inflammatory fibroblast-like synoviocytes (FLS). However, the mechanism by which CP-25 is absorbed by FLS remains unclear.

Comparison of widefield swept-source optical coherence tomography angiography and ultra-widefield fluorescein angiography in the detection of non-perfusion areas in diabetic retinopathy.

Purpose: To compare the detection of non-perfusion areas (NPAs) in diabetic retinopathy (DR) using 24×20 mm widefield swept-source optical coherence tomography angiography (SS-OCTA) and ultra-widefield fluorescein angiography (UWFA), and to explore NPA distribution patterns.

Methods: This retrospective study included 64 eyes from 48 DR patients who underwent 24×20 mm SS-OCTA and UWFA examinations. NPAs were manually annotated, and the detection rates and distribution patterns across retinal quadrants were analyzed and compared between the two imaging modalities.

Development of elite rice with broad-spectrum resistance through pyramiding of key resistance gene and simultaneously editing multiple susceptibility genes.

Knocking out three susceptibility genes (Pi21, Bsr-d1, and Xa5) in a rice breeding line that contains the resistance Piz-t produced enhanced broad-spectrum resistance against the fungal pathogen Magnaporthe oryzae and the bacterial pathogen Xanthomonas oryzae pv. oryzae without obvious growth penalty.

Description of four new synallactid species (Holothuroidea, Synallactida, Synallactidae) from the tropical Western Pacific Ocean.

Four synallactid specimens were collected during four deep-water benthic fauna surveys (2013-2018) conducted at three seamounts ('Jiaolong', 'Y3', and 'M4') and the Ganquan Plateau in the South China Sea, tropical Western Pacific Ocean, at depths ranging from 344 to 3610 m. Morphological examination of these specimens revealed four new species belonging to three genera, which are described as , , , and Notably, represents the first report of an species in the South China Sea. Detailed descriptions are provided for the morphological features, including the type locality, and geographic and bathymetric distributions of these species.

Discovery of mannose as an alternative non-nutrient-deficient regulator of lipid accumulation in microalgae.

Introduction: Microalgae are considered promising bioenergy producers, but their commercial potential is limited by low lipid yields. Nutrient deprivation, particularly nitrogen starvation, is a primary strategy to enhance lipid synthesis efficiency in microalgae. However, controlling this process flexibly, effectively, and accurately remains challenging.

Past, present and future epidemiology of echinococcosis in China based on nationwide surveillance data 2004-2022.

Objectives: We evaluated the epidemiological characteristics of echinococcosis, a global public health threat, in China to inform global control efforts.

Methods: Descriptive, statistical, cluster, spatial, and trend analyses were used to evaluate the epidemiology at national, provincial, and county levels based on 2004-2022 nationwide surveillance data from China.

Results: Between 2004 and 2022, China recorded 72,676 cystic echinococcosis (CE) cases, 11,465 alveolar echinococcosis (AE) cases, and 5703 others, with an average annual cases per million (ANpM) of 3.

Chronic osteomyelitis with pathological fracture induced by infection: a case report and review of the literature.

, commonly residing in the genitourinary tract, can cause rare extragenital infections, especially in immunocompromised individuals. This report details a case of chronic osteomyelitis with a pathological femur fracture in a 79-year-old woman. Despite a history of bone tuberculosis, the infection was identified as through culture and mass spectrometry, highlighting the diagnostic challenges due to the organism's lack of a cell wall.

Recombinant nanobody expressed by transgenic chimeric chickens eliminated vertical transmission of avian leukosis virus subgroup J.

Because of the vertical transmission of avian leukosis virus subgroup J (ALV-J), control of ALV-J in breed of chicken is still a serious issue. Blocking vertical transmission using antibodies is a potential strategy, but its high cost limits its application. We artificially designed recombinant nanobody (Nb) and efficiently expressed and secreted them in three primary chicken cells cultured in vitro by adenovirus delivery.

Potential of Klotho as a Biomarker for Overwork: A Study of Frontline Medical Workers.

Objective: This study evaluates the utility of serum s-αKlotho levels as a quantifiable biomarker for overwork.

Methods: Frontline medical workers aged 20-55 years from Yiling People's Hospital of Yichang were recruited. Criteria included nonsmokers, non-heavy drinkers, no chronic medication use, and no acute illnesses recently.