Novel ultrafine Pt@Fe-MIL-101 nanozyme enables robust aflatoxin B1 immunoassay in diverse marine and agricultural systems.

Background: Aflatoxin B1 (AFB1) stands among the most toxic naturally occurring substances, with its acute toxicity characterized by the induction of acute hepatic necrosis, hemorrhage, and even fatal outcomes, thereby posing a profound threat to human health. Contamination of AFB1 in food commodities can arise at multiple stages throughout the production cycle, including cultivation, storage, and processing. This contamination cascade permeates the entire food supply chain, encompassing primary agricultural products as well as a diverse range of processed food items.

Enhancement of High-Temperature Triboelectric Performance of Polyimide Nanofibers by Introducing Fluorinated Monomers.

Triboelectric nanogenerators (TENGs) are typically constrained to operate below 200 °C due to the thermionic emission effect and material degradation at high temperatures. Herein, high-temperature-resistant fluorinated polyimide nanofibers (4,4'-(hexafluoroisopropylidene) diphthalic anhydride-4,4'-oxidianiline/2,2″-bis(trifluoromethyl)benzidine, 6FDA-ODA/TFDB) were designed to mitigate the thermionic emission effect through the introduction of trifluoromethyl (-CF) groups. 6FDA-ODA/TFDB nanofibers exhibited a fine fiber structure and a large highest occupied molecular orbital-lowest unoccupied molecular orbital (HOMO-LUMO) gap, which enhanced its effective contact area and maintained more localized states for charge transfer.

A three-plasmid-containing CRISPR-Cas9 platform to engineer 916 as an efficient biocontrol agent.

(Bv) is a widely used biocontrol agent against plant diseases, mainly because its genome contains numerous non-ribosomal peptide synthetases (NRPS) gene clusters for the synthesis of various cyclic lipopeptides (CLPs). The domesticated strain Bv916, capable of co-producing four CLPs, has been successfully applied for green control of rice sheath blight and angular leaf spot. To enhance Bv916's biological control efficacy while maintaining environmental safety, it is essential to establish a food-grade gene editing platform in Bv916.

Chronic Heat Stress Caused Lipid Metabolism Disorder and Tissue Injury in the Liver of via Oxidative-Stress-Mediated Ferroptosis.

High-temperature stress has become an important factor that has restricted the aquaculture industry. is a high-economic-value fish that has faced the threat of thermal stress. Based on this point, our investigation aimed to explore the detailed mechanism of the negative impacts of heat stress on the liver metabolism functions in .

Case Report: Secukinumab for the treatment of severe psoriasis in a patient with hereditary hemochromatosis.

There are several comorbidities associated with psoriasis, including genetic disorders such as hereditary hemochromatosis, which can lead to organ damage secondary to iron overload. Herein, we report the case of a 38-year-old Chinese man with hereditary hemochromatosis who received secukinumab for the treatment of severe psoriasis. Follow-up after 3 months showed that the patient's lesions had almost resolved and remained well-controlled for 2 years without any reported side effects.

Exploring the interactions of gut microbiome and metabolites in polycystic ovary syndrome: a review.

Polycystic ovary syndrome (PCOS) is a prevalent endocrine metabolic disorder significantly impacting female health. While its etiology remains incompletely understood, emerging evidence highlights the gut microbiome as a key modulator, affecting not only the gut but also physiology beyond it. Many studies have been committed to exploring the role played by the gut microbiome and have discovered that gut microbiome is commonly dysregulated in PCOS patients.

Human Spinal Cord Microglia/Macrophages Culture: Accutase Digestion and Non-Enzymatic Purification.

Background: Spinal cord injury (SCI) and neurological diseases pose major medical challenges, with microglia/macrophages critical for neuroinflammation and repair. Traditional in vitro models using animal or human brain microglia/macrophages suffer from species/regional differences, limiting translation. The lack of efficient isolation methods for human spinal cord microglia/macrophages (hSCM) has hindered SCI mechanistic research and drug screening.

Simple and robust analysis of cefuroxime in human plasma and bone tissues by LC-MS/MS.

In end-stage osteoarthritis, total joint arthroplasty (TJA) represents the definitive therapeutic intervention. Cefuroxime, a second-generation cephalosporin, exhibits a broad spectrum of activity against both Gram-negative and Gram-positive microorganisms, making it a cornerstone of surgical antimicrobial prophylaxis (SAP) to mitigate prosthetic joint infection (PJI) risk. However, the escalating demand for revision arthroplasties has paralleled rising implant-associated infections, necessitating target-site pharmacokinetic optimization to ensure effective antibiotic exposure at the bone-implant interface.

IIB-DDI: Invariant Information Bottleneck Theory for Out-of-Distribution Drug-Drug Interaction Prediction.

Rapid and accurate identification of drug-drug interactions (DDIs) among multiple medications is crucial for various medical treatments, and clinical therapies. Currently, the increasing significance of molecular substructure interactions in DDI prediction has become a consensus. However, due to the uneven distribution of substructures in molecules and most methods do not consider the differences in substructure distributions, models may rely on spurious substructure relationships, thereby weakening their out-of-distribution (OOD) generalization ability.

TRGH-PPI: Effective and Generalized Prediction of Protein-protein Interactions through Transformer and Graph.

Protein-protein interaction (PPI) is a fundamental means of function and signaling in biological systems. The significant increase in demand and cost associated with experimental PPI research requires computational tools to automatically predict and understand PPI. However, existing methods either heavily rely on protein sequences for PPI prediction, or focus on protein structure based on the belief that structure is the key determinant of interactions.

Cellular Senescence: A Pathogenic Driver for Pulmonary Fibrosis.

Pulmonary fibrosis is a life-threatening progressive lung disease characterized by increased fibrogenesis and decreased lung function. Pulmonary fibrosis has a poor prognosis and a low patient survival rate, with no effective treatments currently available. Cellular senescence is thought to contribute to the pathogenesis of this aging-related disease.

Nanomaterial-Based Biosensors for Aflatoxin B1 Detection: Current Advances, Challenges, and Prospects.

Aflatoxins (AFTs) are mycotoxins recognized as the key contributors to environmental and food contamination. AFTs can contaminate food and raw materials at different phases of manufacturing, transformation, processing, distribution, and marketing. Aflatoxin B1 (AFB1) is the most harmful one among AFTs, and it exhibits potent toxic and carcinogenic effects in animals and humans.

The impact of humility traits on job search behavior in college graduates: the mediating role of job search clarity.

Introduction: This study investigates the influence of humility traits on job search behavior among college graduates, emphasizing the mediating role of job search clarity.

Methods: A total of 406 college graduates were surveyed using the Humility Scale, the Job Search Clarity Scale, and the Job Search Behavior Scale.

Results: The findings revealed that: (1) humility traits, job search clarity, and job search behavior are positively and significantly correlated; (2) humility traits significantly enhance job search clarity; (3) job search clarity significantly positively predicts job search behavior; and (4) job search clarity mediates the relationship between humility traits and job search behavior.

Optimization of Subcritical Water Extraction Process for Total Phenolics From Guava and Investigation of Its Antioxidant Activity and Inhibitory Effect on HepG2 Cell Proliferation.

This investigation utilized subcritical water extraction (SWE) technology to isolate polyphenolic components from Psidium guajava fruits. Process optimization was achieved using response surface methodology (RSM), yielding optimal parameters: a 2.07-h extraction duration, operating temperature of 203°C, and a solvent-to-material ratio of 41:1 mL/g.

Bioinformatics analysis of COMMD family in pan-cancer reveals potential biomarkers and therapeutic targets.

The copper-metabolism Murr1 domain (COMMD) protein family plays an essential role in tumours and the immune system. However, the multi-omics characterisation of COMMD family genes and their role in tumour patients has yet to be explored. We collected data from 33 types of cancer and conducted a comprehensive analysis of the expression abnormalities, diagnostic and prognostic roles, subcellular localisation, pathway enrichment, immune microenvironment and immune checkpoint associations of COMMD family genes in these diseases.

Evaluation of natural prenylflavonoids from Sophora flavescens root bark and their antibacterial activity.

10 compounds (1-10) were isolated from the ethanol extract of Sophora flavescens root bark, including 7 new compounds (1-7). The structures of these compounds were determined through comprehensive spectroscopic analyses, including UV spectroscopy, high-resolution electrospray ionization mass spectrometry (HRESIMS), and nuclear magnetic resonance (NMR), and by comparison with existing literature. Additionally, the antibacterial activity of compounds 1-10 was assessed against eight bacterial strains (four Gram-positive and four Gram-negative) using the micro broth dilution method.

A general transition metal binding aptamer following the Irving-Williams series.

Using both Co and Ni as target metal ions, aptamer selections were carried out by immobilization of a DNA library hybridization. A set of aptamers were obtained although they all showed binding to both metal ions. The Co-1 aptamer was highly enriched in both selections and it is a general metal binding aptamer with binding affinity following the Irving-Williams series: Mn < Fe < Co < Ni < Cu > Zn.

Engineered exosomes with enriched miR-23b suppress the progression of periodontitis by reprogramming macrophages.

Periodontitis is a prevalent inflammatory disease that damages the tooth-supporting structure, leading to tooth instability and loss. Conventional therapies target biofilms but fail to address immune dysregulation, highlighting the need of host-modulating strategies. Herein, the decreased miR-23b level was found to be associated with the progression of periodontitis and identified as a potential therapeutic agent for the disease.

Bacillus spore showcasing key antigens of African swine fever potentializes a promising oral vaccine candidate.

African swine fever (ASF) remains a widespread and fatal infectious disease affecting both domestic pigs and wild boars around the world, and there is still no vaccine available for full protection. In recent years, Bacillus spores have been developed into effective vaccine carriers. Here, Bacillus amyloliquefaciens spores from strain CPLK1314 were used as a vaccine delivery system to display three highly antigenic proteins, p72, p30, and p54 of ASF, and their immune responses in BALB/c mice were evaluated.

CineMyoPS: Segmenting Myocardial Pathologies from Cine Cardiac MR.

Myocardial infarction (MI) is a leading cause of death worldwide. Late gadolinium enhancement (LGE) and T2-weighted cardiac magnetic resonance (CMR) imaging can respectively identify scarring and edema areas, both of which are essential for MI risk stratification and prognosis assessment. Although combining complementary information from multi-sequence CMR is useful, acquiring these sequences can be time-consuming and prohibitive, e.

Epidemiology and clinical features of psoriasis in hard-to-treat body locations: a Chinese nationwide population-based study.

Background: Having psoriasis in hard-to-treat areas, such as the scalp, face, palms, soles, nails, and genitals, can suffer from a reduced quality of life. This study was designed to investigate the prevalence and risk factors of hard-to-treat body locations of psoriasis, and to describe patients' clinical and demographic characteristics, and quality of life impacts.

Methods: We conducted a multicenter observational epidemiological study involving over 1000 hospitals in China, enrolling a total of 7032 psoriasis patients.

A novel necroptosis related prognostic signature for skin cutaneous melanoma based on transcriptome and single cell sequencing analysis.

Necroptosis is a novel programmed cell death that affects the tumor heterogeneity, microenvironment, and prognosis, which is not well elucidated in skin cutaneous melanoma (SKCM). The SKCM-TCGA, GSE65904, and GSE215120 datasets were downloaded from the TCGA and GEO databases, respectively. The necroptosis-related genes were identified by weighted co-expression network analysis (WGCNA) and single-cell sequencing analysis.

High-SNR OAM mode division multiplexing based on delta-sigma modulation using a power domain layer.

Orbital angular momentum (OAM) mode-division multiplexing (MDM) systems exist with mode cross talk, which requires high signal-to-noise ratio (SNR) to improve transmission performance. In this Letter, a novel, to the best of our knowledge, scheme, based on power domain layered delta-sigma modulation (PDL-DSM) was proposed, which composes a high-order signal into two low-order signals and quantizes them using two parallel DSMs. The transmission efficiency of the quantized signals is optimized by using power domain multiplexing, and the quantization noise of the high-power signals is eliminated to improve the overall SNR of the system.

An Efficient Integrator Scheme for Sampling the (Quantum) Isobaric-Isothermal Ensemble in (Path Integral) Molecular Dynamics Simulations.

Because most chemical or biological experiments are performed under conditions of controlled pressure and temperature, it is important to simulate the isobaric-isothermal ensemble at the atomic level to reveal the microscopic mechanism. By extending our efficient configuration sampling approach for the canonical ensemble, we propose a unified "middle" scheme to sample the coordinate (configuration) and volume distribution, which can accurately simulate either classical or quantum isobaric-isothermal processes. Various barostats and thermostats can be employed in the unified "middle" scheme for simulating real molecular systems with or without holonomic constraints.