A general transition metal binding aptamer following the Irving-Williams series.

Using both Co and Ni as target metal ions, aptamer selections were carried out by immobilization of a DNA library hybridization. A set of aptamers were obtained although they all showed binding to both metal ions. The Co-1 aptamer was highly enriched in both selections and it is a general metal binding aptamer with binding affinity following the Irving-Williams series: Mn < Fe < Co < Ni < Cu > Zn.

Dietary inflammatory index and mortality in middle-aged and elderly patients with metabolic syndrome.

Background: Metabolic Syndrome (MetS) significantly increases the risk of cardiovascular diseases and mortality, particularly in middle-aged and elderly populations. The Dietary Inflammation Index (DII) is a validated tool for assessing the inflammatory potential of an individual's diet, with higher scores indicating a dietary pattern that is more favorable to inflammation. The MetS population is inherently characterized by chronic low-grade inflammation, and previous studies have demonstrated an association between the DII and a wide range of chronic diseases in the general population, but little is known about the relationship between dietary inflammation and risk of death in the MetS population.

Effect of Multiple Phosphorus-Nitrogen Flame Retardant on the Properties of PA66.

PA66 is a widely used engineering plastic, but its flammability reduces safety during application. The 9,10-dihydro-9-oxa-10-phosphaphenanthrene 10-oxide (DOPO) and its derivatives are a class of flame retardants with excellent flame-retardant efficiency, which can significantly improve the flame retardancy of PA66. This work synthesized a DOPO derivative flame retardant, DT, containing multiple P/N elements and comprehensively characterized its structure using FTIR and NMR.

Biomimetic sand-fixation-inspired flame retardants: enhanced char stabilization and multifunctional polyurethane with integrated fire safety, radiation shielding, and visual monitoring.

Multifunctional polyurethane (PU) holds significant promise as a next-generation smart material, yet its inherent flammability limits widespread application. Currently, research on condensed-phase flame retardancy primarily focuses on promoting char layer formation, while the critical affecting factor of char layer structural stability is often overlooked. In this study, inspired by the sand-fixation mechanism, a cluster-structured flame retardant was designed using rare earth metals as the central core and phosphoric acids as functional end groups to construct a root-like framework.

A dual inhibitor of TrxR1 and XIAP induces pyroptosis in melanoma.

Malignant melanoma ranks among the most aggressive forms of cancer, with high rates of metastasis and recurrence, as well as a poor prognosis. Consequently, an urgent need is to develop novel precision therapeutic strategies and corresponding drugs. Previous studies have shown that both X-linked inhibitor of apoptosis (XIAP) and thioredoxin reductase 1 (TrxR1) participate in the resistance of melanoma to chemotherapy-induced cell death.

ProgModule: A novel computational framework to identify mutation driver modules for predicting cancer prognosis and immunotherapy response.

Background: Cancer originates from dysregulated cell proliferation driven by driver gene mutations. Despite numerous algorithms developed to identify genomic mutational signatures, they often suffer from high computational complexity and limited clinical applicability.

Methods: Here, we presented ProgModule, an advanced computational framework designed to identify mutation driver modules for cancer prognosis and immunotherapy response prediction.

Increasing Aptamer Affinity from Millimolar to Nanomolar by Forming a Covalent Adduct for Detecting Acrylamide.

Being a neurotoxin and carcinogen, acrylamide has been an important target for developing biosensors. DNA aptamers are attractive for making biosensors due to their programmable structure, low cost, and ease of modification. However, DNA aptamers have poor affinities to low-binding epitope target molecules such as acrylamide.

Bimetallic Pt-Ni nanoparticles with enhanced oxidase like activity for Hg detection in water.

Background: Hg has highly sulfur affinity which was harmful to human health. Noble metal nanomaterials with peroxide-like activity have been widely exploited to develop ultra-sensitive Hg colorimetric detection methods. Unfortunately, using large quantities of HO in these approaches lowered the catalytic activity's high stability, which made the detection signal less reproducible.

Kinetic and Affinity Profiling Rare Earth Metals Using a DNA Aptamer.

Rare earth elements (REEs) are widely used in various high-tech industries. Developing affinity ligands that can detect and distinguish REEs is at the forefront of analytical chemistry. It is also interesting to understand the limits of natural biomolecules for the recognition of REEs.

MXene Bimetallic Coating Synergistic Enhanced Colorimetric-Raman Dual Signal-Based Immunochromatographic Assay for Advancing Detection Performance.

Herein, a three-dimensional thin film-like multifunctional MXene bimetallic coating material (TiC@Au-Ag) with strong color intensity, high surface-enhanced Raman scattering (SERS) activity, and strong antibody affinity (1.00 × 10 M) was prepared. It was the first time that TiC@Au-Ag-based colorimetric-SERS dual-signal immunochromatographic assay (ICA) was developed for the detection of dexamethasone, achieving the limits of detection of 0.

A high affinity and selective DNA aptamer for copper ions.

Capture-SELEX was employed for the selection of DNA aptamers for Cu. The best aptamer named Cu-1 has a of 14.2 nM as determined using the strand-displacement assay, representing an approximate 3000-fold improvement over a previously reported Cu aptamer.

CITMIC: Comprehensive Estimation of Cell Infiltration in Tumor Microenvironment based on Individualized Intercellular Crosstalk.

The tumor microenvironment (TME) cells interact with each other and play a pivotal role in tumor progression and treatment response. A comprehensive characterization of cell and intercellular crosstalk in the TME is essential for understanding tumor biology and developing effective therapies. However, current cell infiltration analysis methods only partially describe the TME's cellular landscape and overlook cell-cell crosstalk.

Comprehensive single-cell profiling of monocytes in HLA-B27-positive ankylosing spondylitis with acute anterior uveitis.

Acute anterior uveitis (AAU) is a common extra-articular manifestation of ankylosing spondylitis (AS), particularly in patients positive for the human leucocyte antigen (HLA)-B27 genetic marker. To explore the underlying mechanisms of HLA-B27 AS-associated AAU, we employed single-cell RNA sequencing to profile the transcriptomes of peripheral blood mononuclear cells in three HLA-B27 AS-associated AAU patients and three healthy controls (HCs). We identified 11 distinct immune cell clusters, with a particular focus on monocytes, revealing six subsets, including three previously unidentified subsets, namely, GTPase immune-associated proteins, Th17-related, and lncRNA monocytes, with unique gene expression patterns.

Preparation of Montmorillonite-Melamine Cyanurate and Inhibition of the Emission of Phosphine from PA6/Aluminum Hypophosphate.

In order to mitigate the release of toxic phosphine from aluminum hypophosphite in twin-screw processing, montmorillonite-melamine cyanurate was prepared by three methods: (1) mechanical intercalation, (2) water intercalation and (3) in situ intercalation. The sheet spacing of montmorillonite was increased from 1.140 nm to 1.

Ganoderma lucidum triterpenoids investigating their role in medicinal applications and genomic protection.

Objectives: Ganoderma lucidum (GL) is a white rot fungus widely used for its pharmacological properties and health benefits. GL consists of several biological components, including polysaccharides, sterols, and triterpenoids. Triterpenoids are often found in GL in the form of lanostane-type triterpenoids with quadrilateral carbon structures.

Rapid screening of prednisolone acetate adulterants in health foods using colloidal gold immunochromatographic assay.

In this study, a rapid detection method utilizing colloidal gold immunochromatography (CG-ICA) was developed for the detection of illegally added prednisone acetate in health foods. Initially, the preparation conditions of colloidal gold solution were optimized. The optimal potassium carbonate dosage, antibody diluent type, antibody dosage, probe labeling time, blocking time and BSA dosage were determined.

Post-synthetic modification fluorescence UiO-66-Eu immunochromatography for high-performance detection of sodium pentachlorophenoate.

Sodium pentachlorophenate (PCP) is widely used as a herbicide, fungicide, or molluscicide. It is highly toxic, easily soluble in water, making it highly prone to diffusion and causing water and soil pollution. Through the food chain, it enters animal bodies and remains in food, causing toxicity to humans and animals.

A specific and ultrasensitive Cas12a/crRNA assay with recombinase polymerase amplification and lateral flow biosensor technology for the rapid detection of .

Unlabelled: The potential of CRISPR/Cas systems for nucleic acid detection in novel biosensing applications is remarkable. The current clinical diagnostic detection of () is based on serological identification, culture, and PCR. We report a rapid, simple, and sensitive method for detecting and screening for .

METTL3 -mediated m6A modification of circ_0000620 regulates cisplatin sensitivity and apoptosis in lung adenocarcinoma via the MiR-216b-5p/KRAS axis.

Circular RNAs (circRNAs) are stable non-coding RNAs characterized by the absence of the conventional 5' cap and 3' polyadenylated tail structure. Its involvement in various aspects of cancers underscores its significance in oncology. Elevated expression of circ_0000620 was observed in both lung adenocarcinoma (LUAD) tissues and cell lines.

A Dual and Rapid RPA-CRISPR/Cas12a Method for Simultaneous Detection of Cattle and Soybean-Derived Adulteration in Goat Milk Powder.

The adulteration of goat milk powder occurs frequently; cattle-derived and soybean-derived ingredients are common adulterants in goat milk powder. However, simultaneously and rapidly detecting cattle-derived and soybean-derived components is still a challenge. An efficient, high-throughput screening method for adulteration detection is needed.

Simple synthesis of flower-like ZnO@Pt composites for dual-mode colorimetric detecting Hg with smartphone and UV-vis.

Background: Mercury is one of the most toxic heavy metal contaminants that can be harmful to human health through the food chain. Recently, the colorimetric detection of heavy metals based on nanozyme catalytic activity has received extensive interest due to the simplicity, signal visibility and suitability for in situ detection. However, the majority of these nanozymes that can be utilized for detecting mercury with high synthesis temperature and complicated synthesis methods, which limited their practical application.

Ultrasensitive TiCTx@Pt-Based Immunochromatography with Catalytic Amplification and a Dual Signal for the Detection of Chloramphenicol in Animal-Derived Foods.

Herein, a catalytic amplification enhanced dual-signal immunochromatographic assay (ICA) based on Pt nanoparticles (Pt NPs) modified with TiCTx MXene (TiCTx@Pt) was first developed for chloramphenicol (CAP) in animal-derived foods. Due to the large specific surface area and abundant active sites of TiCTx@Pt, they can be loaded with hundreds of Pt NPs to enhance their catalytic activity, resulting in a significant increase in the detection sensitivity; the sensitivity was up to 50-fold more sensitive than the reported ICA for CAP. The LODs of the developed method for milk/chicken/fish were 0.