Lipid Regulation of Tea Protein Lipid-Lowering Peptides on 3T3-L1 Preadipocytes and High-Fat Mice.

In this study, tea protein lipid-lowering peptides were prepared by enzymatic hydrolysis, and 3T3-L1 preadipocytes and high-fat mice were used to investigate its in vitro and in vivo lipid regulating effect. The results showed that tea protein lipid-lowering peptides could significantly inhibit the adipogenic differentiation of 3T3-L1 preadipocytes and reduce lipid accumulation. At the peptides concentration of 0.

Systematic review of hypoglycemic polysaccharides: sources, preparation, and identification techniques, evaluation models, structure-activity relationships, and mechanistic insights.

The rising prevalence of diabetes presents a considerable challenge to global public health security. Although hypoglycemic drugs have clear effects, they can cause serious complications, such as liver and kidney dysfunction and hypoglycemia. Natural active polysaccharides are active substances that relieve diabetes and have no toxic or side effects, thus attracting substantial interest.

Enrichment of Zinc-Chelating Peptides from Casein Using IMAC and Their Zinc Binding Modes and Promoting Intestinal Zinc Absorption Activity.

In this study, casein was used as the raw material to prepare casein hydrolysates, and the fraction with high activity (F-IMAC) was enriched by immobilized metal affinity chromatography. Then, F-IMAC and its zinc chelates (F-IMAC-Zn, 99.1 mg/g) were characterized.

Corrigendum: Therapeutic potential of essential oil in modulating inflammation and the gut microbiome.

[This corrects the article DOI: 10.3389/fmicb.2023.

Two Novel β-Casein Iron-Chelating Peptides: Isolation, Identification, Prediction of Iron Binding Modes, and Enhancement Intestinal Iron Absorption Activity.

In this study, β-casein was used to prepare iron-chelating peptides through the enzymatic hydrolysis. β-casein hydrolysates were purified and identified, and combined with molecular docking to screen high-activity iron-chelating peptides. Additionally, their iron binding modes and enhancement of intestinal iron absorption activities were investigated.

Research advancements on theaflavins: Isolation, purification, synthesis, gut microbiota interactions, and applications potentials.

Theaflavins (TFs), specific polyphenolic compounds found in tea, including TF, TF-3-G, TF-3'-G, and TFDG, are renowned for their health-promoting effects. The growing interest in TFs among researchers necessitates a comprehensive review of their properties and impacts. This review systematically examines the chemical and physical properties of TFs, covering their isolation, purification, synthesis, safety, and bioavailability, as well as their implications for health.

Consumption of oleogel alleviates lipid metabolism disorders in high-fat diet-fed rats by inhibiting LPS-induced gut microbiota-mediated inflammation.

This study investigated the effect of oleogel consumption on lipid metabolism, gut microbiota and low-grade inflammation in rats fed with a high-fat diet. Male SD rats received either a control diet or high-fat diets for six weeks. The high-fat diets included a regular high-fat diet and high-fat diets in which lard was replaced with pure sunflower oil, un-gelled sunflower oil containing a dispersed gelator, or gelled sunflower oil with the gelator (oleogel).

Novel immunomodulatory peptides from hydrolysates of the Rana spinosa (Quasipaa spinosa) meat and their immunomodulatory activity mechanism.

In this study, hydrolysates of Rana spinosa meat were purified and characterized, and combined with molecular docking to screen potential immunomodulatory peptides and explore their activities and mechanisms of action. The results showed that 582 peptides were identified from the hydrolysates, and three novel immunomodulatory peptides, GIHETTYNS (1020.4512 Da), IADRMQKE (989.

Fabrication of interfacial crystallized oleogel emulsion for quercetin delivery with enhanced environmental stability and bioaccessibility.

Background: Quercetin is a flavonoid compound with numerous bioactivities. However, the low solubility, easy degradation and low bioaccessibility limit its application. In this study, a novel interfacial crystallized oleogel emulsion was fabricated, where beeswax was used as the oleogelator, for quercetin encapsulation with enhanced stability and bioaccessibility.

Ganoderma lucidum triterpenoids investigating their role in medicinal applications and genomic protection.

Objectives: Ganoderma lucidum (GL) is a white rot fungus widely used for its pharmacological properties and health benefits. GL consists of several biological components, including polysaccharides, sterols, and triterpenoids. Triterpenoids are often found in GL in the form of lanostane-type triterpenoids with quadrilateral carbon structures.

Rapid screening of prednisolone acetate adulterants in health foods using colloidal gold immunochromatographic assay.

In this study, a rapid detection method utilizing colloidal gold immunochromatography (CG-ICA) was developed for the detection of illegally added prednisone acetate in health foods. Initially, the preparation conditions of colloidal gold solution were optimized. The optimal potassium carbonate dosage, antibody diluent type, antibody dosage, probe labeling time, blocking time and BSA dosage were determined.

Engineering crystal network of supramolecular Oleogel via kinetical regulation for improved lutein bioaccessibility.

This study presented an approach for controlling supramolecular oleogel crystal network by regulating kinetical factors - specifically, a combination of cooling temperature and aging period. Results indicated that only under long aging period, supramolecular oleogels prepared at different cooling temperature exhibited distinct crystal morphology compared to those under short aging period. The physicochemical properties of oleogels were affected by different crystal networks.

DNA Barcoding Unveils Novel Discoveries in Authenticating High-Value Snow Lotus Seed Food Products.

Snow Lotus Seed (SLS), esteemed for its nutritional and market value, faces challenges of authentication due to the absence of appropriate testing standards and methods. This results in frequent adulteration of SLS sourced from () with other plant seeds endosperm. Traditional chloroplast DNA barcoding methods are inadequate for species identification due to the absence of chloroplasts in seeds endosperm.

Impact of wheat processing on growth, serum biochemistry, and ruminal microbiota in sheep (Ovis aries).

This study investigated the impact of wheat processing methods (wheat flour vs wheat pellets) on the growth performance, serum biochemical parameters, and rumen microbiome composition in sheep. Results indicated that feeding of wheat flour resulted in significantly higher terminal weight and average daily gain (P < 0.05) and lower cholesterol and ALP04 levels (P < 0.

Association of litter size with the ruminal microbiome structure and metabolomic profile in goats.

The Yunshang black goat is a renowned mutton specialist breed mainly originating from China that has excellent breeding ability with varying litter sizes. Litter size is an important factor in the economics of goat farming. However, ruminal microbiome structure might be directly or indirectly regulated by pregnancy-associated factors, including litter sizes.

Synthesis of Tetrasubstituted Alkenes by Rhodium-Catalyzed Regioselective Cyano Transfer.

We describe herein a novel, general, and robust approach to structurally diversified alkenyl nitriles through a Rh-catalyzed cyano transfer reaction between alkynyl-malononitrile derivatives and aryl/alkenyl boronic acids. This reaction exhibits high chemo- and regioselectivity and a broad substrate scope. The tetrasubstituted alkenyl dinitriles (34 examples, average 58% yield) are obtained through substrate tuning and ligand control.

Investigating the Role of in the Growth of Bovine Preadipocytes: Using Transcriptomic Analyses to Understand Beef Quality.

Intramuscular fat is a crucial determinant of carcass quality traits like tenderness and taste, which in turn is influenced by the proliferation of intramuscular preadipocytes. This study aimed to investigate the Krüppel-like factor 6 (KLF6)-mediated proliferation of bovine preadipocytes and identify underlying molecular mechanisms. Down-regulation of KLF6 by siKLF6 resulted in a significant ( < 0.

Advances in traditional herbal formulation based nano-vaccine for cancer immunotherapy: Unraveling the enigma of complex tumor environment and multidrug resistance.

Cancer is attributed to uncontrolled cell growth and is among the leading causes of death with no known effective treatment while complex tumor microenvironment (TME) and multidrug resistance (MDR) are major challenges for developing an effective therapeutic strategy. Advancement in cancer immunotherapy has been limited by the over-activation of the host immune response that ultimately affects healthy tissues or organs and leads to a feeble response of the patient's immune system against tumor cells. Besides, traditional herbal medicines (THM) have been well-known for their essential role in the treatment of cancer and are considered relatively safe due to their compatibility with the human body.

Effect of BFS1243 on a female frailty model induced by fecal microbiota transplantation in germ-free mice.

Frailty, a complex geriatric syndrome, significantly impedes the goal of achieving 'healthy aging'. Increasing evidence suggests a connection between gut microbiota, systemic inflammation, and disease. However, it remains to be determined whether interventions targeting the intestinal flora can effectively ameliorate frailty.

Interfacial crystallized oleogel emulsion with improved freeze-thaw stability and tribological properties: Influence of cooling rate.

In this study, the influence of cooling rate on the freeze-thaw stability, rheological and tribological properties of interfacial crystalized oleogel emulsion was investigated. Results showed that slower cooling rate could promote formation of larger crystals and stronger network in oleogels. Additionally, oleogel emulsions showed higher freeze-thaw stability than those stabilized solely by emulsifiers.

Hypersampsonone H attenuates ulcerative colitis via inhibition of PDE4 and regulation of cAMP/PKA/CREB signaling pathway.

Background And Objectives: Ulcerative colitis (UC) is a recurrent intestinal inflammatory disease which poses a serious threat to the life of patients. However, there are no specific drugs for UC yet. Hypericum sampsonii Hance (HS) is a Chinese herbal medicine traditionally used to treat enteritis and dysentery.

Improving quality of poultry and its meat products with probiotics, prebiotics, and phytoextracts.

Remarkable changes have occurred in poultry farming and meat processing in recent years, driven by advancements in breeding technology, feed processing technology, farming conditions, and management practices. The incorporation of probiotics, prebiotics, and phytoextracts has made significant contributions to the development of poultry meat products that promote both health and functionality throughout the growth phase and during meat processing. Poultry fed with these substances improve meat quality, while incorporating probiotics, prebiotics, and phytoextracts in poultry processing, as additives or supplements, inhibits pathogens and offers health benefits to consumers.

Advances of Predicting Allosteric Mechanisms Through Protein Contact in New Technologies and Their Application.

Allostery is an intriguing phenomenon wherein the binding activity of a biological macromolecule is modulated via non-canonical binding site, resulting in synchronized functional changes. The mechanics underlying allostery are relatively complex and this review is focused on common methodologies used to study allostery, such as X-ray crystallography, NMR spectroscopy, and HDXMS. Different methodological approaches are used to generate data in different scenarios.

Characterization of key flavor substances and their microbial sources in traditional sour bamboo shoots.

Identifying key flavor compounds and their producing bacteria in sour bamboo shoots is crucial for flavor stabilization and industrial production. This study analyzed 15 traditional sour bamboo shoot samples from northern Guangdong to determine key flavor substances and microbial community. Results showed key flavor substances were acetic acid (RTC ≥ 50% in 10 samples), lactic acid (RTC ≥ 50% in 5 samples), and p-cresol (ROC ≥ 93%).