A new D-π-A-type fluorescent probe for sensitive and quantitative monitoring and bioimaging of NH in various water/soil samples, living cells, plants and zebrafish.

Herein, a new carbazole-based fluorescent probe 3-methyl-2-(2-(9-propyl-9H-carbazol-3-yl)vinyl)benzo[d]thiazol-3-ium iodide (CTB) with a D-π-A structure was exploited for the colorimetric/fluorescent dual-signal detection of hydrazine (NH). CTB showed ultrashort reaction time (30 s), ultrahigh anti-interference, good specificity, outstanding sensitivity (low detection limit: 24.6 nM), and wide pH applicable range (4-11).

Cost-effectiveness analysis of sintilimab additional to chemoradiotherapy in high-risk locoregionally advanced nasopharyngeal carcinoma.

Objectives: The recently released CONTINUUM trial was the first phase 3 randomized study to demonstrate the efficacy and safety of immunotherapy in high-risk locoregionally advanced nasopharyngeal carcinoma (NPC), showing that sintilimab can bring clinical benefits to these populations.

Materials And Methods: We developed a Markov model to assess the cost and effectiveness of sintilimab plus standard therapy versus standard therapy alone. The primary outcomes included total costs, life-years, quality adjusted life years (QALYs) and incremental cost-effective ratios (ICERs).

Sex disparity of liver cancer recurrence following surgery: sex-determining region Y gene promotes immunosuppressive microenvironment and favors metastasis.

Background: Male sex is a risk factor of the development and prognosis of primary liver cancers. However, sex difference of tumor recurrence following curative resection and the underlying mechanism needs to be clarified.

Methods: Sex and outcomes among patients undergoing resection for hepatocellular carcinoma (HCC) or intrahepatic cholangiocarcinoma (ICC) were investigated in two international multi-institutional databases.

Engineering of the WSN Strain for Investigating Antiviral and Antitumor Immunity.

Influenza viruses are highly variable and transmissible, often causing seasonal influenza outbreaks worldwide. The genomic sequence, protein structure and function, and the viral packaging mechanism have been extensively studied and well characterized, positioning influenza viruses as key models for the identification and validation of conditionally controlled genetic elements. With the development of reverse genetics and synthetic biology, genetically engineered influenza viruses can more effectively control virus replication, thereby increasing vaccine safety and inducing a robust immune response in the host.

Engineering of Vesicular Stomatitis Virus (VSV) for Modulating Cell Death and Antitumor Immunity.

Vesicular stomatitis virus (VSV), a negative-sense RNA virus, has emerged as a versatile platform for oncolytic virotherapy and vaccine development. Numerous advantages, such as a broad host range, rapid replication, and simple and easily modifiable genomes, make it an attractive candidate for both the treatment and prevention of infectious diseases and tumors. Advances in molecular biology techniques, particularly reverse genetics and synthetic biology, have facilitated the precise engineering of VSV genomes, thereby significantly expanding their applications in cancer therapeutics.

Machine learning algorithms for the clinical diagnosis of acute atypical asthma exacerbation.

Objective: To develop a predictive model for the clinical diagnosis of acute atypical asthma attacks using machine learning algorithms and investigate the risk factors related to the diagnosis of atypical asthma.

Methods: This study retrospectively collected data on characteristics, symptoms, general examinations, pulmonary functional tests, and FeNO results of patients in the Aerospace Center Hospital. Five machine learning algorithms (logistic regression, decision tree, random forest, support vector machine, extreme gradient boosting) were employed to select variables for predicting outpatient cases of atypical asthma exacerbation in routine practice.

In-depth analysis of the risk factors for persistent severe acute respiratory syndrome coronavirus 2 infection and construction of predictive models: an exploratory research study.

Background: Persistent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection differs from long coronavirus disease (COVID-19) (acute symptoms ≥ 12 weeks post-clearance). The Omicron BA.5 variant has a shorter median clearance time (10-14 days) than the Delta variant, suggesting that the traditional 20-day diagnostic threshold may delay interventions in high-risk populations.

Effects of water-saving irrigation on microbial community structures, assembly, and metabolic activities in alfalfa rhizosphere soils.

In agricultural areas of arid Xinjiang, China, selecting appropriate irrigation strategies for farmland is essential. Increasing attention is being paid to the ecological effects of different irrigation methods on the soil environment. As a crucial component of soil quality, the microbial community is a key indicator of the impacts of irrigation on the soil environment.

Two Novel β-Casein Iron-Chelating Peptides: Isolation, Identification, Prediction of Iron Binding Modes, and Enhancement Intestinal Iron Absorption Activity.

In this study, β-casein was used to prepare iron-chelating peptides through the enzymatic hydrolysis. β-casein hydrolysates were purified and identified, and combined with molecular docking to screen high-activity iron-chelating peptides. Additionally, their iron binding modes and enhancement of intestinal iron absorption activities were investigated.

F-box only protein 10 protects against kidney tubulointerstitial fibrosis by inhibiting ACSL4-mediated lipid peroxidation and ferroptosis.

Renal tubular epithelial cell ferroptosis is significantly linked to kidney tubulointerstitial fibrosis, a critical pathological condition in chronic kidney disease. F-box only protein 10 (FBXO10), a newly identified ferroptosis-regulating gene, plays a role in various pathological contexts; however, its involvement in kidney tubulointerstitial fibrosis is not yet fully understood. This research sought to investigate whether FBXO10 regulates ferroptosis in kidney tubular epithelial cells and its relationship with kidney tubulointerstitial fibrosis in both animal and cellular models.

Optimizing Coordinated Active Sites of Transition Metal Complexes: Exploring Metal-Molecule Interactions for Governing CO-to-CO Conversion.

Syngas (H/CO) is an essential chemical feedstock for industrial products. In these focal points, electrocatalytic CO reduction has emerged as a desirable strategy for realizing effective syngas production to satisfy energy and environmental requirements. In this work, a metal-molecule hybrid electrode with inherent H generation favorability has been crafted by loading molecular Co(Ni)-bpy (bpy = 2,2'-bipyridine) complexes on Ag foil.

Study on the effect of load on the structural response of projectile during penetration process.

To delve deeper into the dynamic behaviors of a typical projectile structure subjected to multi-pulse loading, this paper focuses on the scenario of a projectile positively penetrating an equally spaced, multi-layer target. It proceeds by designing multi-pulse excitation loads that vary in temporal intervals and amplitudes. These designed loads are subsequently incorporated into an experimental model to systematically investigate how load parameters, specifically the pulse period, pulse width, and pulse amplitude, influence the amplification of the projectile structure's vibration response.

[Source Apportionment and Ecological Risk of Soil Heavy Metals in Typical Vanadium-titanium Magnetite Mining Areas Affected by Intensive Transportation Activities in Chengde].

A total of 895 surface soil samples （0-20 cm） and five road-dust samples were collected in a typical vanadium-titanium magnetite mining area of Hongqi-Damiao catchment in the Chengde City and analyzed for 12 heavy metals （Cu, Ni, Cd, Cr, Pb, Zn, Hg, V, Ti, Mn, As, and Co） and their chemical speciation. The accumulation and ecological risk characteristics of soil heavy metals （HMs） were clarified by geological accumulative index, potential ecological risk index （RI）, risk assessment coding （RAC）, and modified Nemerow integrated ecological risk index （mNIER）. Multiple statistical methods including principal component analysis, correlation analysis, cluster analysis, redundancy analysis, and GIS spatial analysis were used to investigate the spatial variation response of soil HMs to the environmental impact factors （distance to the road network, land use, and soil parent material types）.

Exploring the Therapeutic Potential of MIR-140-3p in Osteoarthritis: Targeting CILP and Ferroptosis for Novel Treatment Strategies.

Osteoarthritis (OA) is a prevalent and debilitating joint disorder that affects millions of individuals worldwide, severely impairing mobility, independence, and quality of life. Emerging evidence suggests that ferroptosis is a critical factor in OA pathogenesis. However, its precise involvement and underlying mechanisms remain poorly understood.

Disulfide Bonds Reinforced Self-Assembly of Cellulosic Wastes Toward N/S-Enriched 3D Carbon Foams with Starfish-Like Networks for High-Performance Supercapacitors.

Developing high-performance electrodes derived from cellulosic wastes is an effective strategy for promoting large-scale energy storage and achieving carbon neutrality, yet how to enhance capacitive activity from the perspective of surface-interface structure regulation remains a challenge. Herein, a disulfide bond reinforced self-assembly of cellulosic wastes strategy is demostrated to fabricate 3D carbon foams with thiram and bio-straws as examples. The cellulose-enriched piths of straws (EP) are impregnated with thiram solution followed by pyrolysis, where thiram can form a stable 3D cross-linked networks via disulfide-centered hydrogen bonds reinforced self-assembly of EP and thiram, endowing the obtained starfish-like skeleton connected 3D carbon foams with high N/S contents and hierarchical porous structure.

Electrochemical Lithiation Regulates the Active Hydrogen Supply on Ru-Sn Nanowires for Hydrogen Evolution Toward the High-Performing Anion Exchange Membrane Water Electrolyzer.

Designing a rational electrocatalyst/electrolyte interface with superb active hydrogen supply is of significant importance for the alkaline hydrogen evolution reaction (HER) and anion exchange membrane water electrolyzers (AEMWEs). Here, we propose a strategy to tune the interfacial active hydrogen supply via inducing dissoluble cation into electrocatalysts to boost HER in alkali, with electrochemical lithiated sub-2 nm RuSn nanowires (NWs) as a proof of concept. It is found that a part of Li could dissolve in situ from lithiated RuSn NWs during HER, which tends to affect the interfacial structure and facilitate the proton transport.

Enhancing humification in high-temperature composting: Insights from endogenous and exogenous heating strategies.

Livestock manure is difficult to manage for high moisture and nutrients. High-temperature composting (> 75 °C) reduces moisture. However, the humification process, crucial for nutrient recycling, remains poorly understood.

Tumor microenvironment-responsive and modulatory manganese-based nanoenzyme for enhanced tumor immunotherapy.

The characteristics of the tumor microenvironment (TME) have a close and internal correlation with the effect of cancer immunotherapy, significantly affecting the progression and metastasis of cancer. The rational design of nanoenzymes that possess the ability to respond to and regulate the TME is driving a new direction in catalytic immunotherapy. In this study, we designed a multifunctional manganese (Mn)-based nanoenzyme that is responsive to acidic pH and overxpressed HO at tumor site and holds capability of modulating hypoxic and immunosuppressive TME for synergistic anti-tumor photothermal/photodynamic/immunotherapy.

Early differentiation of committed erythroid cells defined by miR-144/451 expression.

Before committing to an erythroid cell lineage, hematopoietic stem cells differentiate along a myeloid cell pathway to generate megakaryocyte-erythroid biopotential progenitor cells in bone marrow. Recent studies suggest that erythroid progenitors (EryPs) could be generated at the level of common myeloid progenitors (CMPs). However, due to a lack of suitable markers, little is known about the early differentiation of these committed EryP cells during CMP development.

USP5 inhibits anti-RNA viral innate immunity by deconjugating K48-linked unanchored and K63-linked anchored ubiquitin on IRF3.

Interferon regulatory factor 3 (IRF3) is a central hub transcription factor that controls host antiviral innate immunity. The expression and function of IRF3 are tightly regulated by the post-translational modifications. However, it is unknown whether unanchored ubiquitination and deubiquitination of IRF3 involve modulating antiviral innate immunity against RNA viruses.

Ubiquitin-specific protease 1 facilitates tumor immune escape from natural killer cells and predicts the prognosis in small cell lung cancer.

Objective: Small cell lung cancer (SCLC) is commonly recognized as the most fatal lung cancer type. Despite substantial advances in immune checkpoint blockade therapies for treating solid cancers, their benefits are limited to a minority of patients with SCLC. In the present study, novel indicators for predicting the outcomes and molecular targets for SCLC treatment were elucidated.

The deep learning radiomics nomogram helps to evaluate the lymph node status in cervical adenocarcinoma/adenosquamous carcinoma.

Objectives: The accurate assessment of lymph node metastasis (LNM) can facilitate clinical decision-making on radiotherapy or radical hysterectomy (RH) in cervical adenocarcinoma (AC)/adenosquamous carcinoma (ASC). This study aims to develop a deep learning radiomics nomogram (DLRN) to preoperatively evaluate LNM in cervical AC/ASC.

Materials And Methods: A total of 652 patients from a multicenter were enrolled and randomly allocated into primary, internal, and external validation cohorts.

CIP2A inhibitors TD52 and Ethoxysanguinarine promote macrophage autophagy and alleviates acute pancreatitis by modulating the AKT-mTOR pathway.

Background: Acute pancreatitis (AP) is a prevalent and serious condition within the digestive system, with approximately 20 % to 30 % of cases advancing to severe acute pancreatitis (SAP). During the initial phases of SAP, macrophages are activated in response to the substantial amounts of acinar cell contents and damage-associated molecular patterns (DAMPs) resulting from acinar cell destruction. Subsequently, activated macrophages release a significant array of pro-inflammatory factors that exacerbate the progression of SAP.

N6-methyladenosine RNA modification promotes Severe Fever with Thrombocytopenia Syndrome Virus infection.

Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV), a novel bunyavirus primarily transmitted by Haemaphysalis longicornis, induces severe disease with a high mortality rate. N6-methyladenosine (m6A) is a prevalent internal chemical modification in eukaryotic mRNA that has been reported to regulate viral infection. However, the role of m6A modification during SFTSV infection remains elusive.