Food traceability analysis and quality marker monitoring in Ophiocordyceps sinensis with multimodal ensemble learning.

Ophiocordyceps sinensis (OS) faces serious risks of food fraud, including quality misrepresentation, adulteration and illegal additives. To preserve the economic interests of consumers and the transparent management of food trade, so this study proposed a rapid and non-destructive detection tool to identify traceability of the growth environment and predict quality markers of OS. Colors, textures and spectra were utilized to build unimodal models, respectively.

Unraveling Enhanced Superconductivity in Single-Layer FeSe through Substrate Surface Terminations.

Single-layer FeSe on SrTiO(001) substrates shows a superconducting transition temperature much higher than that of bulk FeSe, which has been attributed to factors such as electron doping, interfacial electron-phonon coupling, and electron correlations. To pinpoint the primary driver, we grew single-layer FeSe films on SrTiO(001) substrates with coexisting TiO and SrO surface terminations. Scanning tunneling spectroscopy revealed a larger superconducting gap (17.

A three-plasmid-containing CRISPR-Cas9 platform to engineer 916 as an efficient biocontrol agent.

(Bv) is a widely used biocontrol agent against plant diseases, mainly because its genome contains numerous non-ribosomal peptide synthetases (NRPS) gene clusters for the synthesis of various cyclic lipopeptides (CLPs). The domesticated strain Bv916, capable of co-producing four CLPs, has been successfully applied for green control of rice sheath blight and angular leaf spot. To enhance Bv916's biological control efficacy while maintaining environmental safety, it is essential to establish a food-grade gene editing platform in Bv916.

A biopiezocatalyst harnessing mechanical energy to enhance bioplastic production from CO and organic carbon.

The sustainable bioproduction of chemicals from CO remains far from reaching its full potential. The productivity of autotrophic bioprocesses could benefit from harnessing ubiquitous mechanical energy sources, which are inaccessible for energizing bioproduction systems to this day. In this work, we develop a hybrid system where the efficient piezocatalyst zinc oxide (ZnO) harnesses mechanical vibration to stimulate the growth of the chemolithoautotrophic bacterium Cupriavidus necator and its production of the bioplastic polyhydroxybutyrate (PHB) from CO.

The analysis of landscape design and plant selection under deep learning.

This paper explores the application of deep learning (DL) techniques in landscape design and plant selection, aiming to enhance design efficiency and quality through automated plant leaf image recognition (PLIR). A novel framework based on Convolutional Neural Network (CNN) and Fully Convolutional Network (FCN) is proposed. This framework integrates multi-scale feature fusion, attention mechanisms, and object detection technologies to improve the recognition of landscape elements and the selection of plant leaves.

Development and validation of a multimodal automatic interictal epileptiform discharge detection model: a prospective multi-center study.

Background: Visual identification of interictal epileptiform discharge (IED) is expert-biased and time-consuming. Accurate automated IED detection models can facilitate epilepsy diagnosis. This study aims to develop a multimodal IED detection model (vEpiNetV2) and conduct a multi-center validation.

Nutritional and immune-inflammatory scoring system for predicting outcomes in newly diagnosed diffuse large B-cell lymphoma patients.

Background: Diffuse large B-cell lymphoma (DLBCL) is the most common aggressive non-Hodgkin lymphoma and often carries a poor prognosis. Current prognostic systems such as the International Prognostic Index (IPI) and National Comprehensive Cancer Network (NCCN)-IPI do not incorporate patients' nutritional, immune, or inflammatory status, which may affect outcomes.

Methods: We retrospectively analyzed 423 newly diagnosed DLBCL patients and collected 12 clinical indicators reflecting nutritional, immune, and inflammatory status.

PABPC1 SUMOylation enhances cell survival by promoting mitophagy through stabilizing U-rich mRNAs within stress granules.

Stress granules (SGs) are cytoplasmic, membraneless organelles that modulate mRNA metabolism and cellular adaptation under stress, yet the mechanisms by which they regulate cancer cell survival remain unclear. Here, we identify Poly(A)-Binding Protein Cytoplasmic 1 (PABPC1), a core SG component, as stress-inducible SUMOylation target. Upon various stress conditions, SUMOylated PABPC1 promotes SG assembly and enhances cancer cell survival.

Targeted sequencing and functional interrogation identified novel variant at 12q14.2 associated with risk of ovarian cancer in Han Chinese women.

Chromosome 12q14.2 has been reported as a potential risk locus for epithelial ovarian cancer (EOC) in genome-wide association study (GWAS). We performed targeted sequencing around the rs11175194 at chromosome 12q14.

Developing Dynamic Ion Transport Channels in Polymer Solid Electrolytes for High-Performance Lithium Metal Batteries.

Solid polymer electrolytes (SPEs) are promising for next-generation solid-state lithium metal batteries owing to their electrochemical stability and high safety. However, limited ionic conductivity and poor interfacial stability have hindered their practical applications. To address these challenges, we propose a novel approach by incorporating trace amounts of sulfone (SL) into polyacrylic-based SPEs, purposely creating ".

[Relationship between nurses' sleep quality and quality of life in class Ⅲ grade a hospitals in Lanzhou in 2023: the chain mediating role of social support and psychological resilience].

Objective: To explore the relationship between sleep quality and quality of life of nurses in tertiary hospitals, and to verify the chain-mediated role of social support and psychological resilience.

Methods: A questionnaire survey was conducted from November to December 2023 on 732 nurses from three tertiary hospitals in Lanzhou City, China, using cross-sectional whole cluster sampling. The Quality of Life Scale(SF-36), the Psychological Resilience Scale(CD-RISC), the Pittsburgh Sleep Quality Index(PSQI), and the Social Support Rating Scale(SSRS) were applied to measure their quality of life, psychological resilience, quality of sleep, and social support levels.

Characterization of vaginal microbiota across female reproductive phases.

Background: The vaginal microbiota plays a crucial role in women's reproductive health, but its composition and function throughout different female reproductive phases remain poorly understood. This study aimed to investigate the dynamic variations in vaginal microbial communities corresponding to different hormonal states throughout the female reproductive phase.

Methods: Vaginal samples were collected from 150 healthy women across five reproductive phases: follicular phase, luteal phase, early pregnancy, lactation, and menopause (30 samples per group).

Associations of Leukocyte-Derived Chemotaxin-2 (LECT2) with Insulin Resistance and Metabolic Syndrome in Type 2 Diabetes: A Cross-Sectional Study.

Objective: Leukocyte-derived chemokine-2 (LECT2) is a novel neutrophil chemokine synthesized by the liver. This research aimed to investigate the potential link between LECT2 levels and metabolic syndrome (MetS) as well as insulin resistance (IR) in patients with type 2 diabetes mellitus (T2D). The results could offer fresh perspectives on the diagnosis and therapeutic strategies for T2D and associated metabolic disorders.

Optimization and Evaluation of Colchicine Patch Formulation Based on Box-Behnken Design.

This study aimed to optimize colchicine (COL) transdermal patches to enhance key quality attributes, including adhesion, drug release, and transdermal penetration. The goal was to develop a formulation with improved performance based on BOX-Behnken Design (BBD) optimization. The BBD method was used to design experiments evaluating the effects of three key variables: COL content, penetration enhancer content (azone and propylene glycol), and the solvent evaporation time at 80 °C.

Bacillus spore showcasing key antigens of African swine fever potentializes a promising oral vaccine candidate.

African swine fever (ASF) remains a widespread and fatal infectious disease affecting both domestic pigs and wild boars around the world, and there is still no vaccine available for full protection. In recent years, Bacillus spores have been developed into effective vaccine carriers. Here, Bacillus amyloliquefaciens spores from strain CPLK1314 were used as a vaccine delivery system to display three highly antigenic proteins, p72, p30, and p54 of ASF, and their immune responses in BALB/c mice were evaluated.

The non-metabolic function of 6PGD coordinates CCNA2 and HMGA2 expression to drive colorectal cancer progression and drug response.

Emerging evidence demonstrates that the metabolic and non-metabolic functions of metabolic enzymes play a key role in tumorigenesis and progression. 6-phosphogluconate dehydrogenase (6PGD) is a key metabolic enzyme in the pentose phosphate pathway (PPP), which displays aberrant expressions and functions in cancer. However, whether 6PGD serves non-metabolic functions in regulating cancer progression and drug response has not been reported.

Study on multi-dimensional dynamic changes and quality control of Aurantii Fructus during the stir-frying with bran process.

Aurantii Fructus (AF), a medicine-food homology substance, is valued for its distinctive aroma and health-promoting properties, and has been widely used in food, condiments, and traditional Chinese medicine. However, the raw form's irritating properties limit its direct application, and stir-frying with bran mitigates this drawback, enhancing its suitability for food applications. This study investigated the dynamic changes in AF's multi-dimensional parameters (including physical, chemical, and biological) during the stir-frying with bran process, aiming to elucidate the underlying processing mechanisms.

Airway Organoid Models as Pivotal Tools for Unraveling Molecular Mechanisms and Therapeutic Targets in Respiratory Diseases: A Literature Review.

Respiratory inflammatory and infectious diseases continue to impose a substantial global health burden, compounded by persistent gaps in understanding their pathogenic mechanisms and limited therapeutic advancements. To address these challenges, this review systematically analyzed literature from PubMed, Web of Science, and Scopus databases (2005-2025) to evaluate the evolution and applications of airway organoid models in respiratory disease research. Key findings include: (1) the convergence of traditional culture techniques with advanced methodologies - including 3D matrix embedding, bioprinting and organoids-on-chips technologies - has enabled unprecedented recapitulation of human airway architecture and multicellular interactions; (2) these novel models provide unique insights into disease pathogenesis, host-microbe dynamics, and drug response variability; (3) the inherent capacity to maintain native cellular diversity and disease-associated phenotypes positions airway organoids as crucial platforms for personalized medicine approaches.

CXCR4 Clustering Induced by Polymeric Nanothreads Impedes Cancer Cell Metastasis via PIEZO1-Mediated Mechanotransduction.

The way in which the antagonist binds to the target molecule affects the intervention of its downstream pathway. Previously, an antagonistic strategy for CXC chemokine receptor 4 (CXCR4) utilizing two polymeric nanothreads is developed that self-assemble into patches on the cell surface. These patches induce CXCR4 clustering, leading to a significantly enhanced antagonism compared to conventional monovalent or multivalent receptor binding.

Anti-angiogenic therapy combined with immune checkpoint blockade mediates CCR7 + CD8 + T-cell entry into HCC through high endothelial venules.

Background And Aims: HCC remains a leading cause of cancer mortality in China. Anti-angiogenic drugs (AADs) plus immune checkpoint blockade (ICB) combination therapy shows considerable promise for HCC; however, its efficacy is hampered by immunosuppression within the tumor microenvironment. High endothelial venules (HEVs) facilitate lymphocyte migration and tumor infiltration.

Enhanced lymphatic transportation of SLN by mimicking oligopeptide transportation route.

Solid lipid nanoparticles (SLN) could enhance the oral bioavailability of loaded protein and peptide drugs through lymphatic transport. Natural oligopeptides regulate nearly all vital processes and serve as a nitrogen source for nourishment. They are mainly transported by oligopeptide transporter-1 (PepT-1) which are primarily expressed in the intestine with the characteristics of high-capacity and low energy consumption.

AI-chemometric assisted real-time monitoring of tryptophan fermentation process using a sensor fusion strategy.

Tryptophan, an essential amino acid, crucially impacts neuronal function, metabolism, immunity, and gut homeostasis. Microbial fermentation is the mainstream method for tryptophan production. The precise production process is essential for ensuring both high quality and optimal yield.

Glucose affects capsular polysaccharides synthesis via CcpA and HPr in Streptococcus pneumoniae.

Streptococcus pneumoniae is a conditionally pathogenic bacteria that colonizes the nasopharynx of 27% to 65% of children and 10% of adults. Capsular polysaccharides are the most critical virulence factor of S. pneumoniae, and nonencapsulated strains are usually non-pathogenic.