Aloe emodin functionalized dual-channel label with self-adhesion and hydrophobicity for visual monitoring of seafood freshness.

In this study, aloe emodin and β-d-glucose pentaacetate were added into potato starch/polyvinyl alcohol for the construction of colorimetric/fluorescent dual-pass intelligent response labels. Inspired by the lotus leaf structure, retained the advantages of the label itself and solved the hydrophilic problem of the label, and further developed a multi-functional dual-channel smart label with hydrophobicity and self-adhesion. The water contact angle of the prepared T-AEB label was 120.

Polycystic ovary syndrome: A metabolic disorder with therapeutic opportunities.

Polycystic ovary syndrome (PCOS) is a highly prevalent endocrine disorder characterized by intertwined reproductive and metabolic abnormalities. While its causal origins remain incompletely understood, accumulating evidence suggests metabolic dysfunctions-manifested by insulin resistance, obesity, hyperglycemia, and dyslipidemia-as key contributors to the pathogenesis and progression of PCOS. Emerging interventions targeting these metabolic disturbances, including caloric restriction, GLP-1-based therapies, and bariatric surgery, have shown efficacy in alleviating PCOS symptoms and potentially blocking their inheritance.

Out-of-Office Blood Pressure Measurements in Patients with Heart Failure Registry Study (OOBPM-HF study) in China: study protocol, rationale and design.

Background: The optimal blood pressure (BP) target for patients with heart failure (HF) remains a topic of debate. The relationship between out-of-office BP measurements and the prognosis of HF patients is still unclear. However, there is a lack of large-scale studies investigating out-of-office BP levels in Chinese populations with HF.

Identification of resistance to stripe rust in 267 Chinese spring wheat landraces germplasm and molecular detection of disease resistance genes.

Stripe rust ( f. sp. ) poses a major threat to Chinese wheat production.

Screening for endocrine hypertension in China: protocol for a multicentre prospective cohort study.

Introduction: Secondary hypertension accounts for 5%-10% of all hypertensive patients. Among these conditions, endocrine hypertension, such as primary aldosteronism (PA), Cushing's syndrome (CS) and pheochromocytoma and paraganglioma (PPGL), is a common and significantly harmful cause. With advancements in diagnostic techniques, the prevalence of endocrine hypertension is much higher than previously reported, but large-scale epidemiological survey data in this field are still lacking in China.

Visual and rapid detection of glyphosate in tea using a MOF-driven fluorescent paper sensor.

To overcome the limitations of conventional enzymatic glyphosate (Glyp) detection methods, including enzyme environmental sensitivity, high cost, and limited enzyme sources, this study developed a portable paper-based fluorescence platform (Y-R membrane) based on a metal-organic framework (MOF)-driven fluorescence regulation mechanism for non-enzymatic and rapid detection of Glyp. This platform was constructed by immobilizing MnMOF and yellow carbon dots (Y-CDs) on a polyvinylidene fluoride membrane. The ZrOx-OH derived from alkaline-treated UiO-66 regulated the Mn/Mn redox reactions through its surface hydroxyl groups, triggering the red fluorescence of MnMOF.

Bidirectional association of daily steps with sarcopenia: a longitudinal study.

Background: Step count is a well-established measure of physical activity, and step-based recommendations offering practical public guidance. However, the bidirectional associations between step count and sarcopenia remain unclear, limiting the application of step-based interventions for sarcopenia prevention. This study examined the directional association between daily steps and sarcopenia.

Development of a measurement of doctor-patient communication quality scale.

Background: The number of medical disputes in China has been increasing, highlighting the urgent need to improve doctor-patient relationships.

Objective: To improve doctor-patient relationships and enhance communication, a scale for measuring doctor-patient communication quality (DPCQ) was developed.

Methods: Based on existing relevant foreign scales, questionnaire items were designed to collect patients' perceptions of doctor-patient communication quality.

Cyclometallated Ir(III) complexes exhibit antitumor activity through angiogenesis inhibition.

Targeting angiogenesis remains a pivotal therapeutic approach in cancer treatment, aiming to impede tumor growth and metastasis. In this study, three novel cyclometallated Ir(III) complexes (Ir1, Ir2, and Ir3) were synthesized and systematically assessed for their in vitro cytotoxic effects across a range of cancer cell lines. Mechanistic studies revealed that these complexes are internalized by MCF-7 cells via energy-dependent pathways and predominantly accumulate within lysosomes or the endoplasmic reticulum.

A Systematic Survey of the Optimal Strategy for Dealing With Missing Binary Outcomes in Simulation Studies of Randomized Controlled Trials.

Aim: To summarize the optimal strategies for dealing with missing binary outcome data (MBOD) in randomized controlled trials (RCTs) as informed by simulation studies, and to summarize the quality of reporting in these studies.

Methods: To identify simulation studies comparing at least two strategies to deal with MBOD and evaluating their performance (bias, coverage and power), we searched MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials via Ovid, Web of Science, and JSTOR from their inception up to December 20, 2023. We evaluated reporting quality using established criteria for simulation studies in medical statistics.

Sonodynamic Therapy-Based DNA Nanocarriers with Hypoxia-Inducible Factor-1α Silencing Activation for Precision Lung Cancer Therapy.

As lung cancer is still the deadliest cancer worldwide, there is an urgent need for safer and more efficient therapies. This study aims to address the challenges posed by tumors in reducing the efficacy of sonodynamic therapy (SDT) through mechanisms such as hypoxia and abnormal blood vessel formations. In this study, manganese-containing DNA nanoflowers (DHA-DDF) loaded with doxorubicin (DOX) were functionalized with an AS1411 aptamer and a hypoxia-inducible factor-1α (HIF-1α) antisense sequence.

Efficacy and tolerability of pramipexole versus antidepressants for Parkinson's disease with depression: a meta-analysis of randomized controlled trials.

Background: Pramipexole is thought to exert antidepressant effects in patients with Parkinson's disease (PD) and depression, but its efficacy compared with typical antidepressants is unclear. This meta-analysis aims to directly compare the safety and efficacy of pramipexole and antidepressants for depression in PD patients.

Methods: Twelve electronic databases including Cochrane, PubMed, Embase, Web of Science, CINAHL, PsycINFO, ProQuest, Open Grey, CBM, The CNKI, Wan Fang Data and VIP, and two clinical trial registry websites were systematically searched up to December 30, 2024.

Impact of cell disruption technologies on the structure, functionality, and digestibility of Cordyceps militaris protein isolates.

Cell disruption is essential for extracting intracellular proteins from fungal biomass. However, its effects on the structural and functional properties of proteins remain insufficiently understood. This study used Cordyceps militaris as a model to evaluate three cell disruption techniques-high-pressure homogenization (HPH), ultrasonication (US), and cellulase hydrolysis-for extracting C.

RCA-HCR cascade mediated-linear multi-shear site DNA walker (LMDW) to construct an electrochemical biosensor for ultrasensitive detection of Tumor markers.

Herein, through the cascade integration of the RCA and HCR reactions, an innovative linear multi-shear site DNA walker (LMDW) with high walking efficiency was developed to fabricate an electrochemical biosensing platform for detecting microRNA-21 (miRNA-21) associated with breast cancer. Impressively, compared with conventional single-shear site DNA walkers with poor walking efficiency, the LMDW obtained from the RCA-confined HCR reaction can accommodate a considerable number of functional domains, increasing the shear efficiency to the hairpin probe with the beacon material, thereby consequently enhancing the reaction rate and sensitivity of the biosensor. Following validation, the probe acquisition time down to just 20 min, far less than traditional single-shear site DNA walkers and the detection limit was 0.

Photocontrolled Programmable Enzymatic Cascade for Robust CRISPR Diagnostics.

CRISPR-Cas12a-based diagnostic technologies have revolutionized nucleic acid detection, but their broader application remains constrained by the protospacer adjacent motif (PAM) requirement and limited multiplexing capabilities due to reliance on -cleavage. Here, we present a photocontrolled programmable enzymatic cascade strategy that enables temporal regulation of three sequential reactions─nucleic acid amplification, photoactivated lambda exonuclease (λ-exon)-mediated single-stranded DNA (ssDNA) generation, and PAM-independent Cas12a detection─all within a one-pot system, effectively overcoming the PAM constraint. We further exploit the orthogonal -cleavage activity of Cas12a and Cas13a to enable simultaneous dual-gene detection within the one-pot system, thereby circumventing multiplexing limitations.

Unraveling the Relevance of Graphene-Fluid Hydrodynamic Coupling on the Exfoliation of Graphite in Water.

Liquid-phase exfoliation via shear flow is a widely adopted technique for the large-scale production of graphene. However, the underlying nano- and microscale exfoliation mechanisms remain poorly understood. In this work, we address this issue by performing hybrid nonequilibrium hydrodynamic simulations of coarse-grained defect-free graphite nanoplatelets immersed in a mesoscopic water fluid via the lattice Boltzmann method.

Targeting RPS16 with cinnamaldehyde to suppress cervical cancer: inhibition of the PI3K/AKT pathway and degradation of HPV E6 and E7 proteins.

Cinnamaldehyde (CA), a naturally occurring compound that can be isolated from various plants within the genus Cinnamomum, exhibits antitumor properties but lacks mechanistic clarity in cervical cancer (CVC). This study systematically investigated CA's therapeutic potential against CVC through integrated in vitro and in vivo approaches. In HeLa cells, CA demonstrated selective cytotoxicity (cell counting kit-8 (CCK-8) /colony formation assays) and suppressed migration (wound healing assay), outperforming other CVC cell lines.

Plasma lipoprotein subclasses and risk of incident knee osteoarthritis: A population-based cohort study.

Objective: Lipoprotein subclasses play diverse roles in health and disease. Although lipoproteins have been implicated in knee osteoarthritis (KOA), the relationship between lipoprotein subclasses and KOA remains unexplored.

Method: We conducted a cohort study using UK Biobank data to examine the associations of 85 plasma lipoprotein subclasses, defined by combinations of density (high, low, intermediate, very-low), size (extremely-large, very-large, large, medium, small, very-small), and composition (triglyceride, free cholesterol, cholesteryl ester, phospholipid), with incident KOA in metabolome-wide analysis.

Enhancing frozen-thawed embryo transfer outcomes and treatment personalization through machine learning models.

Background: Infertility affects millions globally, with significant social, emotional, and economic consequences. While frozen-thawed embryo transfer (FET) is a cornerstone of assisted reproductive technology, its clinical pregnancy success rates remain inconsistent (29.6-59.

Effects of different exercise modalities on balance performance in healthy older adults: a systematic review and network meta-analysis of randomized controlled trials.

Background: Exercise has been proven to significantly enhance balance performance in healthy older adults, particularly those living independently in community settings. However, it remains unclear which specific types of exercise are the most effective and which modality yields the best intervention outcomes. We aimed to evaluate the effects of different exercise modalities on four balance-related outcomes-Berg Balance Scale (BBS), Timed Up and Go Test (TUG), gait speed (GS), and one-leg stance with eyes open (OLSEO)-in healthy older adults, using a systematic review and network meta-analysis.

Efficacy and safety of acupuncture therapies for adult patients with mild and moderate major depressive disorder: A systematic review and meta-analysis.

Background: Acupuncture therapy provides a complementary and alternative approach to treating major depressive disorder (MDD), but its efficacy and safety have still not been comprehensively assessed. Recently published systematic reviews remain confusing and inconclusive.

Objective: This systematic review evaluated the efficacy and safety of acupuncture therapy alone or combined with antidepressants for adult patients with mild and moderate MDD.

Well-Defined Redox-Active Hyperbranched Polymers for Flow Batteries: Harnessing Self-Condensing Vinyl Copolymerization by Flow Chemistry.

The increased adoption of renewable power necessitates the development of grid-scale storage solutions, with aqueous redox flow batteries (RFBs) at the forefront. Despite their potential, performance limitations arising from high solution viscosity at high concentrations of active material and rapid degradation due to active material crossover continue to pose challenges. Here, we use flow chemistry to create redox-active hyperbranched copolymers (HBCs), which exhibited substantially improved suppression of crossover and enhanced rheology behaviors.

Genome-wide association study of pre-harvest sprouting resistance and grain color in common wheat (Triticum aestivum L.).

Background: Pre-harvest sprouting (PHS) is a serious problem in wheat production globally. Grain color (GC) has a notable impact on PHS resistance, red grains typically show higher resistance compared to white grains. To understand the genetic factors influencing PHS and GC, a genome-wide association study (GWAS) was conducted on a natural population of 235 wheat cultivars using a 90 K single nucleotide polymorphism (SNP) arrays.