RCA-HCR cascade mediated-linear multi-shear site DNA walker (LMDW) to construct an electrochemical biosensor for ultrasensitive detection of Tumor markers.

Herein, through the cascade integration of the RCA and HCR reactions, an innovative linear multi-shear site DNA walker (LMDW) with high walking efficiency was developed to fabricate an electrochemical biosensing platform for detecting microRNA-21 (miRNA-21) associated with breast cancer. Impressively, compared with conventional single-shear site DNA walkers with poor walking efficiency, the LMDW obtained from the RCA-confined HCR reaction can accommodate a considerable number of functional domains, increasing the shear efficiency to the hairpin probe with the beacon material, thereby consequently enhancing the reaction rate and sensitivity of the biosensor. Following validation, the probe acquisition time down to just 20 min, far less than traditional single-shear site DNA walkers and the detection limit was 0.

Efficacy and safety of acupuncture therapies for adult patients with mild and moderate major depressive disorder: A systematic review and meta-analysis.

Background: Acupuncture therapy provides a complementary and alternative approach to treating major depressive disorder (MDD), but its efficacy and safety have still not been comprehensively assessed. Recently published systematic reviews remain confusing and inconclusive.

Objective: This systematic review evaluated the efficacy and safety of acupuncture therapy alone or combined with antidepressants for adult patients with mild and moderate MDD.

SARS-CoV-2-Induced Phosphorylation of HSPA9 at Ser627: Potential Implications for Mitochondrial Function, Cell Cycle Regulation, and Immune Evasion.

Heat shock proteins (HSPs), particularly those in the HSP70 family, play essential roles in maintaining cellular homeostasis and orchestrating stress responses, including those triggered by viral infections. Based on data mining of published datasets and experimental characterization, this study identified HSPA9 phosphorylation at serine 627 (S627) as a potential regulatory site associated with SARS-CoV-2 infection. Our findings demonstrate that phospho-S627 HSPA9 enhances mitochondrial function and mass, potentially meeting the elevated energy demands of viral replication.

Poly(ADP-ribose) polymerase-1 (PARP1)-based dual inhibitors targeting cancer synthetic lethality (2022-present): advances and perspectives.

Poly(ADP-ribose) polymerase 1 (PARP1), a critical enzyme in DNA damage repair (DDR) and genome stability maintenance, has emerged as a pivotal therapeutic target in oncology. PARP1 inhibitors (PARPi) exploit synthetic lethality to selectively target homologous recombination repair (HRR)-deficient cancer cells, revolutionizing treatment for patients with breast cancer susceptibility gene 1/2 (BRCA1/2) mutations and other DNA repair defects. However, monotherapy resistance and limited efficacy in broader patient populations necessitate innovative strategies.

Novel ATR/PARP1 Dual Inhibitors Demonstrate Synergistic Antitumor Efficacy in Triple-Negative Breast Cancer Models.

Concomitant inhibition of ataxia telangiectasia and Rad3-related protein (ATR) and poly ADP-ribose Polymerase (PARP) pathways is a promising strategy in cancer therapy, potentially expanding the clinical utility of ATR inhibitor (ATRi) and PARP inhibitor (PARPi). A novel series of ATR/PARP1 dual inhibitors is developed through the pharmacophore fusion of AZD6738 and Olaparib. Among them, B8 emerges as the most promising candidate, exhibiting potent ATR (IC: 17.

Perirenal fat as a potential marker and therapeutic target for metabolic syndrome: insights from a multicenter randomized controlled trial.

Background: Metabolic syndrome (MetS) represents a constellation of metabolic abnormalities. Perirenal fat is a type of visceral fat surrounding the kidneys and possesses distinct anatomical and physiological features. This study aims to investigate the association between perirenal fat volume (PrFV) and MetS in Chinese adults.

Hippocampal CA1 neuron, a crucial regulator for chronic stress exacerbating Alzheimer's disease progression.

Chronic stress, a common risk factor for psychiatric disorders, is also implicated in the pathogenesis of Alzheimer's disease (AD). However, its underlying mechanisms remain elusive. Here, we provide evidence for chronic restraint stress (CRS), a widely used stress model in rodents, to regulate AD pathology.

Carotene hydroxylase DcCYP97A3 affects carotenoid metabolic flow and taproot color by influencing the conversion of α-carotene to lutein in carrot.

The color diversity of non-purple carrot taproots is mainly affected by carotenoid species and content. Carrot cytochrome P450 carotene β-ring hydroxylase (DcCYP97A3) may influence carotenoid accumulation in carrots; however, the roles of in carrot remain unclear. Compared to the orange carrot 'Kurodagosun, KRD', the yellow carrot 'Yellowstone, YST' had greater relative transcript levels of .

Duration of primary/secondary treatment to prevent recurrent venous thromboembolism: a systematic review and meta-analysis.

Antithrombotic therapy can prevent recurrent deep vein thrombosis (DVT) and pulmonary embolism (PE). It is, however, associated with an increased risk for major bleeding. This meta-analysis systematically reviewed the evidence regarding the duration of antithrombotic therapy to assess benefits and harms.

Bo's abdominal acupuncture treatment for adult-onset Still's disease: A case report.

Background: Adult-onset Still's disease (AOSD) is a rare autoinflammatory disease characterized by nonspecific symptoms such as fever, rash, sore throat and arthralgia. This paper reports a clinical case of AOSD successfully treated with Bo's abdominal acupuncture (BAA).

Case Summary: We report a 20-year-old man who suffered from cold exposure, presenting with high fever, rash, sore throat, arthralgia, and elevated erythrocyte sedimentation rate, leukocytosis with neutrophilic predominance, elevated ferritin, elevated C-reactive protein, and negative rheumatoid factors.

Does the charitable medical assistance program impact catastrophic medical expenditures for families of children with leukemia? An evidence-based study in China.

Background: Pediatric leukemia is the most prevalent childhood cancer in China, exerting a considerable financial impact on affected families. Despite the mandatory participation of all Chinese children in the Resident Basic Medical Insurance, out-of-pocket (OOP) expenses remain substantial for families of children with leukemia. However, charity assistance has been shown to help mitigate these financial burdens.

Variation of nanoparticles in pickering emulsion optimize the physical, control-release and sustained antioxidant properties of hydroxypropyl methylcellulose based microporous film.

The proper encapsulation of liposoluble tea polyphenols (LTP) is expected to better protect oil system. Chitosan hydrochloride-carboxymethyl starch (CHC-CMS) nanoparticles-based Pickering emulsions and hydroxypropyl methylcellulose/sodium citrate (HPMC/SC) microporous film were combined to embed and control-release LTP. With the CHC:CMS ratio varied from 1:0.

An Efficient Bifunctional Core-Shell ZIF-90@ZIF-67 Composite as a Stable Pickering Interfacial Catalyst for the Deacetalization-Knoevenagel Tandem Reaction.

Exploiting advanced solid particles is crucial to the construction of Pickering emulsions catalysis. Recently, metal-organic frameworks (MOFs) have been used as ideal emulsifiers stabilizing Pickering emulsions for interfacial catalysis. Although Pickering emulsions stabilized by core-shell MOFs have significant importance in practical studies, to date there have been very limited reports on this topic.

Finely ordered intracellular domain harbors an allosteric site to modulate physiopathological function of P2X3 receptors.

P2X receptors, a subfamily of ligand-gated ion channels activated by extracellular ATP, are implicated in various physiopathological processes, including inflammation, pain perception, and immune and respiratory regulations. Structural determinations using crystallography and cryo-EM have revealed that the extracellular three-dimensional architectures of different P2X subtypes across various species are remarkably identical, greatly advancing our understanding of P2X activation mechanisms. However, structural studies yield paradoxical architectures of the intracellular domain (ICD) of different subtypes (e.

Association between late sleeping and major adverse cardiovascular events in patients with percutaneous coronary intervention.

Background: Sleeping late has been a common phenomenon and brought harmful effects to our health. The purpose of this study was to investigate the association between sleep timing and major adverse cardiovascular events (MACEs) in patients with percutaneous coronary intervention (PCI).

Methods: Sleep onset time which was acquired by the way of sleep factors questionnaire in 426 inpatients was divided into before 22:00, 22:00 to 22:59, 23:00 to 23:59 and 24:00 and after.

Emotional differences based on comments on doctor-patient disputes with varying levels of severity.

Background: The risks associated with negative doctor-patient relationships have seriously hindered the healthy development of medical and healthcare and aroused widespread concern in society. The number of public comments on doctor-patient relationship risk events reflects the degree to which the public pays attention to such events.

Aim: To explore public emotional differences, the intensity of comments, and the positions represented at different levels of doctor-patient disputes.

Au(I)/Sc(III)-co-catalyzed tandem spiroannulation/cycloisomerization of 3-(2-ethynylaryl)--tosylaziridines with indoles to access 5-benzo[]carbazoles.

An unusual spiroannulation/cycloisomerization cascade of 3-(2-ethynylaryl)--tosylaziridines with indoles enabled by cooperative gold/scandium catalysis is presented, which facilitates the synthesis of 5-benzo[]carbazoles in moderate to excellent yields. This protocol features a broad substrate scope and good functional-group compatibility. Additionally, the resulting 5-benzo[]carbazoles exhibit good fluorescence properties, demonstrating the synthetic practicality of this method.

Unlocking Efficient Hydrogen Production: Nucleophilic Oxidation Reactions Coupled with Water Splitting.

Electrocatalytic water splitting driven by sustainable energy is a clean and promising water-chemical fuel conversion technology for the production of high-purity green hydrogen. However, the sluggish kinetics of anodic oxygen evolution reaction (OER) pose challenges for large-scale hydrogen production, limiting its efficiency and safety. Recently, the anodic OER has been replaced by a nucleophilic oxidation reaction (NOR) with biomass as the substrate and coupled with a hydrogen evolution reaction (HER), which has attracted great interest.