Probiotics and non-starch carbohydrates as microecological agents: A review of classification strategies and feed applications.

Over recent decades, the indiscriminate use of antibiotics in animal production to enhance product quality and maximize economic returns has raised critical concerns. However, antibiotic misuse has led to the development of antimicrobial resistance in livestock and poses substantial health risks to humans through drug residue accumulation. In response, nations globally have progressively implemented bans on antibiotic inclusion in animal nutrition, redirecting scientific attention toward antibiotic-free feed additives that maintain or enhance animal health performance.

Metabolomics reveals key biomarkers for ischemic stroke: a systematic review of emerging evidence.

Objective: To systematically collate and evaluate metabolomics-based biomarkers of ischemic stroke (IS) to guide clinical diagnosis and treatment.

Methods: Comprehensive literature searches were conducted in PubMed, Embase, and Web of Science using "IS" and "metabolomics" as core keywords, covering publications up through February 2024. Any original metabolomic research related to IS was selected.

Qu-yu-jie-du decoction maintenance therapy improves postoperative survival in metastatic colorectal cancer: a single-center randomized trial with 36-month follow-up.

Background: Qu-yu-jie-du (QYJD) decoction is an established traditional Chinese medicine (TCM) formulation. For over ten years, there has been empirical evidence in support of its benefits for colorectal cancer patients, yet RCT validation for it as a postoperative maintenance therapy is lacking. Despite achieving no evidence of disease (NED) status after curative-intent resection in metastatic colorectal cancer (mCRC), up to 60 % of patients relapse within five years, with no guideline-recommended maintenance therapy.

Assessment of ovarian dysfunction induced by environmental toxins: a systematic review.

Objective: This study systematically assess the potential impact of various environmental pollutants as chemical, airborne, and heavy metal on ovarian function in women, focusing on ovarian reserve such as anti-Müllerian hormone (AMH) and antral follicle count (AFC) as well as hormone levels like follicle-stimulating hormone (FSH) and estradiol (E2). By reviewing epidemiological evidence, this research aims to elucidate the reproductive toxicity of these pollutants and provide scientific support for public health policy to protect reproductive health in women of childbearing age.

Methods: Following the PRISMA-P guidelines, a comprehensive search was conducted in PubMed, EMBASE, Cochrane Library, and Web of Science databases to include all relevant studies up to July 30, 2024.

Acupuncture for diabetic peripheral neuropathy: A systematic review and Bayesian network meta-analysis.

Background: Diabetic peripheral neuropathy (DPN) has emerged as a global health challenge with increasing prevalence rates over the past 3 decades. Acupuncture has been increasingly utilized for the treatment of DPN in recent years. However, whether any specific acupuncture intervention should be considered a priority in the treatment of patients is still unclear.

From complexity to consensus: A roadmap for neutrophil classification.

Neutrophils, previously considered a homogeneous immune cell population, exhibit substantial heterogeneity. Their diverse phenotypic and functional states are shaped by tissue microenvironments and disease-specific signals. However, the lack of robust fate-mapping methods and standardized classification criteria has led to overlapping and ambiguous descriptions of neutrophil heterogeneity.

The effectiveness of the chronic disease management for hypertension: A systematic review and meta-analysis.

Background: Chronic disease management (CDM) is becoming more crucial in optimizing the management of hypertension and related risk control while enhancing the self-efficacy of patients. However, there is a lack of systematic summaries on the subject. This study aimed to provide synthesized evidence on the effectiveness of interventions and the quality of evidence for the implementation of CDM for hypertension.

Adjunctive transcranial direct current stimulation for cognitive improvement in schizophrenia: insights from a systematic review and exploratory meta-analysis.

Introduction: To objectively and accurately evaluate the clinical efficacy of transcranial direct current stimulation (tDCS) for schizophrenia, we conducted this systematic review and exploratory meta-analysis (PROSPERO: CRD420251002632).

Methods: Firstly, randomized controlled trials (RCTs) related to the adjunctive use of tDCS for schizophrenia were searched on PubMed, Embase and Web of Science databases according to search strategies developed in advance. Secondly, the eligible articles were screened according to the inclusion and exclusion criteria.

Global Prevalence of Deep Vein Thrombosis in Patients with Spinal Cord Injury: A Systematic Review and Meta-Analysis.

A meta-analysis of deep vein thrombosis (DVT) in patients with spinal cord injury (SCI) was performed using five databases (PubMed, Embase, the Cochrane Library, Web of Science, and Scopus) from 2000 to March 2023. Observational descriptive studies investigating the prevalence of DVT among patients with SCI were included. Data were retrieved by author, country, continent, gender, age, sample source, and additional variables.

[Exercise preconditioning alleviates motor deficits in MPTP-induced Parkinsonian mice by improving mitochondrial function].

Parkinson's disease (PD) is a common neurodegenerative disorder mainly related to mitochondrial dysfunction of dopaminergic neurons in the midbrain substantia nigra. This study aimed to investigate the effects of exercise preconditioning on motor deficits and mitochondrial function in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse model. Eight-week-old male C57BL/6J mice were randomly divided into four groups: sedentary + saline (SS), sedentary + MPTP (SM), exercise + saline (ES), and exercise + MPTP (EM) groups.

CARE extension guideline for acupuncture case reports.

Existing reporting checklists lack the specificity and comprehensiveness required to effectively guide the documentation of acupuncture case reports. Therefore, we developed a reporting guideline tailored specifically for acupuncture case reports, building upon the CAse REport (CARE) statement. A multidisciplinary group of international experts including clinicians, researchers and methodologists was convened to draft the initial checklist in accordance with the methodology recommended by the Enhancing the QUAlity and Transparency Of health Research (EQUATOR) network.

Single-Cell RNA Transcriptomics and Multi-omics Analyses Reveal the Clinical Effects of Acupuncture on Methadone Reduction.

Opioid use disorders (OUDs) pose a substantial global health burden, with methadone maintenance treatment (MMT) widely adopted as an intervention; however, MMT is associated with immunosuppression, metabolic disturbances, and dysbiosis of the gut microbiota. Despite the potential of acupuncture in reducing methadone dosages and opioid addiction, the underlying biological mechanisms remain unclear. Therefore, we aimed to integrate clinical trial data with multi-omics analysis, including single-cell sequencing, transcriptomics, metabolomics, and metagenomics, to evaluate the effects of acupuncture in patients undergoing MMT.

Autism spectrum disorders and the volume of the striatum and Amygdala: A Mendelian randomization study.

Backgrounds/aims: A number of studies have documented alterations in the structure and function of the striatum in individuals diagnosed with autism spectrum disorder (ASD). Nevertheless, the precise genetic mechanisms underpinning the relationship between autism spectrum disorder (ASD) and striatum and amygdala volume remain to be elucidated. The objective of this study was to estimate the causal effect of ASD on striatum and amygdala volume.

In Situ High-Temperature Raman Spectroscopy and Simulations for Correlating Structure Evolution with Viscosity in the Binary Potassium Titanate System.

The quantitative distribution of various clusters in molten binary KO·TiO ( = 0.5, 1, 2, 3, 4, and 6) was investigated by in situ high-temperature Raman spectroscopy and quantum chemical (QC) calculation. A series of titanium-oxygen model clusters were constructed, and their symmetric stretching vibrational bands and the corresponding Raman scattering cross-sections of titanium nonbridging oxygen were calculated.

Overexpression of DWF1 Enhances Low-Nitrogen Stress Tolerance in Potato Plants.

Nitrogen is an important nutrient required for plant growth and development, but most of the time plants face nitrogen deficiency, all it is important to study the mechanism of low nitrogen tolerance in plants. This study addresses this gap by investigating the role of the gene through the generation and analysis of transgenic potato lines overexpressing (OE1, OE2, OE3). Exogenous BL treatment showed that the gene responded to oleuropein lactone.

[CXCR3 counteracts cisplatin-induced muscle atrophy by regulating E3 ubiquitin ligases, myogenic factors, and fatty acid β-oxidation pathways].

This study aims to explore the role and mechanism of CXC chemokine receptor 3 (CXCR3) in cisplatin-induced skeletal muscle atrophy. Wild-type mice were divided into two groups: cisplatin group and control group (treated by normal saline). The results showed that, compared to the control group, the expression levels of CXCR3 mRNA and protein were significantly up-regulated in the skeletal muscle of the cisplatin group, suggesting that CXCR3 may play an important role in the model of cisplatin-induced skeletal muscle atrophy.

Characterizing the immune landscape of tumor-infiltrating lymphocytes in non-small cell lung cancer.

Tumor-Infiltrating Lymphocytes (TILs) immunotherapy is a highly promising treatment for Non-small Cell Lung Cancer (NSCLC), which is responsible for 18% of all cancer-related deaths. The heterogeneity of TILs remains poorly understood. Here, we utilized combined single-cell RNA (scRNA)/T cell receptor sequencing (scTCR-seq) data from lung adenocarcinoma (LUAD) patients.

Condensed tannin ameliorates intestinal damage and mitochondrial dysfunction induced by high-fat diets in largemouth bass (Micropterus salmoides).

This study investigated the effects of condensed tannin (CT) supplementation on the intestinal health and mitochondrial function in largemouth bass (Micropterus salmoides) fed a high-fat diet. Six experimental diets were formulated, comprising three lipid levels in the diets (10 %, 15 %, and 20 %) with or without 3.75 g/kg CT supplementation.

From feed formulation to frozen flesh: Hydrolysable tannin mitigates high-fat diet-induced quality deterioration in flesh of largemouth bass (Micropterus salmoides) through enhancing antioxidant capacity, optimizing nutrient composition, and textural characteristics.

This study aimed to systematically evaluate the impact of adding hydrolysable tannin (HT, 1.25 g/kg) to a high-fat diet on the flesh quality of fish (Micropterus salmoides) after 12 weeks rearing. Results showed that high-fat diet supplemented with HT increased the content of crude protein and n-3 polyunsaturated fatty acids and optimized the amino acid composition of flesh.

Eicosapentaenoic acid (EPA) reduced lipopolysaccharide-stimulated inflammatory response of RAW264.7 cells via the miR-125b-5p/CREB axis.

Inflammation represents an adaptive physiological response of body immune system to infection or tissue damage, which may be regulated by food supplementation. Eicosapentaenoic acid (EPA) has multi-functions and its anti-inflammatory effect has gained great attention. This study aimed to address the exact molecular mechanism underlying its inflammatory control.

Branched-Chain Amino Acids and Inflammation Management in Endurance Sports: Molecular Mechanisms and Practical Implications.

Endurance athletes frequently experience muscle damage and inflammation due to prolonged, high-intensity exercise, which can impair recovery and hinder performance. This review examines the role of branched-chain amino acid (BCAA) supplementation in muscle repair, inflammation modulation, and immune regulation. BCAAs-particularly leucine and isoleucine-activate key molecular pathways, including the mechanistic target of rapamycin (mTOR) and AMP-activated protein kinase (AMPK), to promote muscle protein synthesis and enhance energy metabolism.

Discovery of biological markers for schizophrenia based on metabolomics: a systematic review.

Introduction And Methods: To discover biomarkers for schizophrenia (SCZ) at the metabolomics level, we registered this systematic review (CRD42024572133 (https://www.crd.york.

Duration of primary/secondary treatment to prevent recurrent venous thromboembolism: a systematic review and meta-analysis.

Antithrombotic therapy can prevent recurrent deep vein thrombosis (DVT) and pulmonary embolism (PE). It is, however, associated with an increased risk for major bleeding. This meta-analysis systematically reviewed the evidence regarding the duration of antithrombotic therapy to assess benefits and harms.

A donor PD-1CD8 T-like regulatory subset mobilized by G-CSF alleviates recipient acute graft-versus-host-disease.

Donor selection determines the occurrence of acute graft-versus-host-disease (aGVHD) following allogeneic hematopoietic stem cell transplantation (allo-HSCT). To optimize the current clinical donor selection criteria and identify putative donor lymphocyte subsets associated with better recipient outcomes, we analyzed the peripheral CD4 and CD8 subsets in 80 granulocyte colony-stimulating factor (G-CSF) mobilized donors and examined the aGVHD incidence of the corresponding 80 haploidentical and identical allo-HSCT recipients. The G-CSF-induced expansion of subsets varied among donors.