Electroacupuncture alleviates intestinal ischemia-reperfusion-induced acute lung injury via the vagus-sympathetic nerve pathway.

Aims: Intestinal ischemia-reperfusion (II/R) injury predominantly causes acute lung injury (ALI), and in severe instances, acute respiratory distress syndrome, both associated with high mortality. Electroacupuncture (EA) excels in regulating autonomic nervous system balance and safeguarding organ function. This study delved into EA's impacts and mechanisms on II/R-induced ALI.

Dynamic Profiling of Lipoteichoic Acid (LTA) and/or Lipopolysaccharide (LPS) Positive Extracellular Vesicles in Plasma as Diagnostic and Prognostic Biomarkers for Bacterial Infection.

Bacterial extracellular vesicles (EVs) are emerging as promising biomarkers for diagnosing bacterial infections. This study explores the diagnostic potential of plasma EVs carrying bacterial lipopolysaccharide (LPS) or lipoteichoic acid (LTA). Using immunoelectron microscopy (IEM) and nanoflow cytometry (nano-FCM), LTA is confirmed on cytoplasmic membrane vesicles (CMVs) from Gram-positive bacteria and LPS on outer membrane vesicles (OMVs) from Gram-negative bacteria, and quantified LTA-positive or LPS-positive (LTA/LPS) EVs in plasma.

Aptamer-functionalized magnetic covalent organic frameworks for rapid extraction and LC-MS/MS quantification of gentamicin in milk.

Gentamicin (GEN) residues in food products, arising from its extensive use in veterinary medicine, have prompted stringent regulations and a demand for simple, rapid detection methods. In this study, aptamer-modified magnetic covalent organic frameworks (COFs), denoted as TpDBD@Fe₃O₄@apt, were synthesized and employed as adsorbents in magnetic solid-phase extraction (MSPE) for the highly specific capture and enrichment of GEN residues in milk. Several factors influencing MSPE efficiency were systematically evaluated and optimized.

Harnessing electroacupuncture: a promising strategy against sleep deprivation-exacerbated post-cardiac arrest brain injury.

Cardiac arrest (CA)-induced post-cardiac arrest brain injury (PCABI) represents a critical contributor to global mortality and neurological disability. While sleep deprivation (SD) is recognized to aggravate neurological outcomes, its role in PCABI pathogenesis remains underexplored. This study investigated the mechanisms by which SD exacerbates PCABI and evaluated the neuroprotective efficacy of electroacupuncture (EA).

Pharmacokinetics and tissue distribution analysis of ginsenoside Rh in rats using a novel LC-MS/MS quantification strategy.

Introduction: Ginsenoside Rh3 (GRh), a rare ginsenoside, demonstrates diverse pharmacological activities ; however, the lack of pharmacokinetic and tissue distribution data has limited its translation to applications. This study aimed to develop and validate a novel liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying GRh in rat biological matrices and to characterize its pharmacokinetic profile and tissue distribution following oral administration.

Methods: A validated LC-MS/MS method was established for the quantification of GRh in rat plasma and tissues.

Dynamic Profiling of Penicillin-Binding Protein 2a (PBP2a)-Positive Extracellular Vesicles: Implications for Early Diagnosis and Treatment Monitoring of Methicillin-Resistant Staphylococcus Aureus Infections.

Infections caused by methicillin-resistant staphylococci (MRS), such as methicillin-resistant Staphylococcus aureus (MRSA), pose significant challenges to public health. The early detection of MRS infections and monitoring of antibiotic resistance profiles are critical for patient management and infection control strategies. Extracellular vesicles (EVs) have emerged as promising biomarkers in infectious disease.

Species and Phylogenetic Diversity of Woody Plants Shift With the Elevational Gradient in Subtropical Forests in South China.

The distribution of biodiversity along elevational gradients and the drivers of these patterns are research hotspots in community ecology; nonetheless, these aspects remain insufficiently understood. To address this, we established 24 plots along an elevational gradient from 300 to 1400 m on Daming Mountain, Guangxi, China, and examined the patterns and drivers of species and phylogenetic diversity along this gradient via polynomial regression, generalized linear mixed model, correlation analysis, and redundancy analyses. With increasing elevation, species and phylogenetic diversity showed a hump-shaped trend, and the phylogenetic structures exhibited clustering at both low and high elevations, whereas at mid-elevations, a coexistence of clustered and overdispersed structures was observed.

A new hybrid neurodynamics-based model-less solution for redundant robot fault-tolerant motion planning and control.

For tasks utilizing redundant manipulators, the motion of multiple joints is involved in performing tracking control. In some cases, the failure of one or more joints may lead to task failure or even cause damage, highlighting the necessity of fault tolerance as a crucial capability for robotic control systems. To achieve the fault-tolerant control capability of the redundant manipulator, a quadratic programming problem is formulated to minimize the joint velocity based on the task-priority strategy.

Integrative analysis identifies IL-6/JUN/MMP-9 pathway destroyed blood-brain-barrier in autism mice via machine learning and bioinformatic analysis.

Autism Spectrum Disorder (ASD) is a complex neurodevelopmental condition characterized by social communication deficits and restricted, repetitive behaviors. Growing evidence implicates neuroinflammation-induced blood-brain barrier (BBB) dysfunction as a key pathogenic mechanism in ASD, although the underlying molecular pathways remain poorly understood. This study aimed to identify critical genes linking BBB function and neuroinflammatory activation, with the ultimate goal of evaluating potential therapeutic targets.

Precision Screening and Surgical Resection of Pan-Cancer Using a Tandem-Locked NIR-II Fluorescent Probe with Optimized Activation Efficiency.

The development of fluorescent probes for cancer detection and imaging that balance high sensitivity, precision, and broad applicability remains a significant challenge. Although "dual-locked" probes have been devised to enhance diagnostic accuracy via two biomarkers, most fall short in sensitivity, response time, and generalizability for pan-cancer use. We address these gaps with ACy-H-NTR, a cascaded-activation, doubly quenched NIR-II probe.

Validation of Biomarkers and Immunotherapy With Crohn's Disease Using WGCNA and Two-Sample Mendelian Randomization Study.

Crohn's disease (CD) is a chronic systemic inflammatory disease that mainly affects the intestine, accompanied by extraintestinal symptoms and immune problems. The progression of the disease may cause permanent damage to the structure and function of the intestine. Due to unclear early symptoms and lack of precise detection methods, early diagnosis of CD is difficult.

Sparse group LASSO and nonlinear machine learning for frequency-feature optimization in noninvasive blood glucose monitoring via bioimpedance spectroscopy.

Diabetic patients need to test their blood glucose levels (BGL) frequently; however, traditional methods of blood collection and testing cause great pain to patients. In order to improve the quality of life of patients, this paper develops a noninvasive, portable, and continuous monitoring blood glucose detection system, which uses the latest bioimpedance integrated circuit to obtain the bioimpedance spectrum (BIS) of the inner forearm of the human body. The obtained BIS covers most of the frequencies up to 1 MHz.

Association between niacin intake and chronic kidney disease in male participants-a cross-sectional study from the NHANES (2005-2018).

Background: Chronic kidney disease (CKD), a significant health challenge in the United States, often progresses from asymptomatic conditions to advanced stages and exhibits a higher prevalence among male individuals. Niacin is known for its metabolic and antioxidant roles, potentially influencing CKD progression. The association between niacin intake and CKD has been rarely investigated in male participants.

Nano-Selenium supplementation Improves Meat Quality, Protein Deposition, and Selenoprotein Gene Expression in Channel Catfish (Ictalurus Punctatus).

Selenium (Se) is a vital trace element for aquatic organisms. This study investigated the impact of dietary nano-selenium (Nano-Se) on growth, muscle nutritional quality, protein deposition, and selenoprotein gene expression in channel catfish (Ictalurus punctatus). A total of 12,000 catfish (85.

Antibiotic-conjugated antimicrobial peptides for enhanced bacterial inhibition.

Herein, we report a pair of paenipeptin C'-based antimicrobial linear lipopeptides that significantly enhance bacterial inhibition through conjugation to antibiotics. When co-incubated with or , these peptides induce bacterial death. The antimicrobial peptides target negatively charged bacterial membranes electrostatic interactions, subsequently disrupting membrane integrity through aggregation and insertion, leading to membrane rupture and cytoplasmic leakage, as evidenced by bacterial morphology studies.

A Novel Hybrid Peptide VLP-Aβ Exhibits Antioxidant Activity In Vitro and In Vivo via KEAP1-NRF2-ARE Signaling Pathway.

Oxidative stress plays a crucial role in the development and progression of various diseases. Antioxidant peptides have attracted great attention in agricultural, food, and clinical fields due to their low toxicity, high efficacy, and easy absorption, but the development of antioxidant peptides and their in-depth molecular mechanisms are still lacking. The previous study established a platform for the high-throughput design and screening of multifunctional peptides and successfully identified a novel hybrid peptide, VLP-Aβ (VA), which exhibits both antioxidant and immunomodulatory properties.

Antibiotic for community-acquired pneumonia in hospitalized adults: A systematic review and Bayesian network meta-analysis.

Background: Inconsistent results have been obtained with various antibiotics for community-acquired pneumonia (CAP) in hospitalized adults. We performed a Bayesian network meta-analysis to compare and rank the effectiveness of antibiotics for adult patients with CAP.

Methods: The databases of PubMed, Embase, and the Cochrane library were systematically searched until May 2025, for randomized controlled trials (RCTs) comparing at least two antibiotics for hospitalized adults with CAP.

Virtual Screening Combined with Phase Transition-FRET for Discovery of Small-Molecule FcRn Antagonists.

Neonatal Fc receptor (FcRn) antagonists, which competitively block the binding of immunoglobulin G (IgG) to FcRn, offer a promising therapeutic strategy for treating IgG-mediated autoimmune disorders. However, all currently available FcRn antagonists are macromolecular therapeutic antibodies, which come with certain clinical limitations. The development of alternative small-molecule FcRn antagonists is therefore of considerable importance.

Trends and predictions of the global burden of ischemic heart disease in women of childbearing age attribute to high body mass index and hypertension,1990-2021: a systematic analysis for the Global Burden of Disease Study.

Background: High body mass index (BMI) and hypertension are quite prevalent in women of childbearing age (WCBA) and are also common risk factors for ischemic heart disease (IHD). However, there are few studies globally evaluating the burden of IHD of WCBA attribute to high BMI and hypertension.

Methods: The DALYs (Disability-adjusted life years), Deaths, YLDs (Year lived with disabilitys), YLLs (Year of life losts) of IHD in WCBA attributable to high BMI and hypertension were analyzed by age, sex, year, and geographical location and Socio-demographic Index (SDI).

Body mass index and clinical outcomes in breast cancer patients undergoing endocrine therapy: A meta-analysis and Mendelian randomization study.

Objective: The relationship between breast cancer prognosis, Body Mass Index (BMI), and endocrine therapy outcomes remains inconclusive. This study examines BMI's impact on survival outcomes in breast cancer patients receiving endocrine therapy through Mendelian randomization (MR) and a comprehensive clinical data review.

Methods: A meta-analysis of clinical studies up to January 2024 investigated the association between obesity and the efficacy and safety of endocrine therapy.

Simultaneous co-delivery of Ginsenoside Rg3 and imiquimod from PLGA nanoparticles for effective breast cancer immunotherapy.

Breast cancer is a fatal malignancy facing human health, with most patients experiencing recurrence and resistance to chemotherapy. The immunosuppressive tumor microenvironment (TME) greatly limits the actual outcome of immunotherapy. This study aimed to develop a modality of theranostics nanoparticles for breast cancer based on a near-infrared light-triggered nanoparticle for the targeted delivery of ginsenoside Rg3 and immune adjuvants imiquimod (R837) for effective breast cancer immunotherapy.

HANDSON Hand: Strategies and Approaches for Competitive Success at CYBATHLON 2024.

A significant number of people with disabilities rely on assistive devices, yet these technologies often face limitations, including restricted functionality, inadequate user-centered design, and a lack of standardized evaluation metrics. While upper-limb prosthetics remain a key research focus, existing commercial solutions still fall short of meeting daily reliability and usability needs, leading to high abandonment rates. CYBATHLON integrates assistive technologies into daily living tasks, driving innovation and prioritizing user needs.

The role of the dopamine system in autism spectrum disorder revealed using machine learning: an ABIDE database-based study.

This study explores the diagnostic value of dopamine system imaging characteristics in children with autism spectrum disorder. Functional magnetic resonance data from 551 children in the Autism Brain Imaging Data Exchange database were analyzed, focusing on six dopamine-related brain regions as regions of interest. Functional connectivity between these ROIs and across the whole brain was assessed.

Construction of hierarchically porous covalent organic frameworks with enhanced enzyme accessibility for sensitive dopamine detection.

Immobilization of fragile enzymes is vital to expanding its application in the extracellular environment. Covalent organic frameworks (COFs), as a class of emerging porous materials, are promising platforms for enzyme immobilization owing to their high porosity and tunable structure. However, the interior pores of COFs often fail to play their roles because of inaccessibility, resulting in decreased performance of immobilized enzymes.