Electroacupuncture alleviates intestinal ischemia-reperfusion-induced acute lung injury via the vagus-sympathetic nerve pathway.

Aims: Intestinal ischemia-reperfusion (II/R) injury predominantly causes acute lung injury (ALI), and in severe instances, acute respiratory distress syndrome, both associated with high mortality. Electroacupuncture (EA) excels in regulating autonomic nervous system balance and safeguarding organ function. This study delved into EA's impacts and mechanisms on II/R-induced ALI.

Genome-Wide Simplification of the AcMNPV Genome Using Synthetic Biology.

Large-scale genome simplification represents a fundamental goal in synthetic biology. Baculoviruses, with their biphasic life cycle and inherent genomic plasticity, have emerged as ideal models for synthetic genome engineering. Although modified baculovirus genomes are widely used as expression vectors for robust recombinant protein production, many genomic regions are dispensable for in vitro budded virus (BV) production.

Discovery of Antidepressant Active Compounds from Polygala Tenuifolia Willd.: An Integrated Multitarget Screening Strategy Combining Network Pharmacology with Cell Membrane Chromatography Coupled to High Performance Liquid Chromatography.

Ethnopharmacological Relevance: Polygala tenuifolia Willd. (YZ) is a traditional Chinese herb that is commonly used in the treatment of psycho-emotional disorders and managing depression. However, the complex chemical composition of this herb poses challenges for separating and identifying the constituents, and there have been few investigations on the bioactive compounds.

A phase 1b/2 study of intermittent talazoparib plus temozolomide in patients with metastatic castration-resistant prostate cancer and no mutations in DNA damage response genes.

As monotherapy, PARP inhibitors have little cytotoxic effect in tumors without homologous recombinant repair (HRR) alterations. Supported by preclinical models, we hypothesized that the PARP inhibitor talazoparib in combination with temozolomide chemotherapy could induce DNA damage leading to cell death and tumor response in patients with metastatic castration-resistant prostate cancer (mCRPC) without HRR alterations. In this phase 1b/2 trial (NCT04019327; registration date July 11, 2019), patients with progressive mCRPC without HRR mutations who failed at least one androgen receptor signaling inhibitor were enrolled in escalating doses of intermittent talazoparib plus temozolomide to determine the maximum tolerated dose and recommended phase 2 dose (RP2D) in Phase 1b.

[Comparison on chemical components of Angelicae Sinensis Radix before and after wine processing by HS-GC-IMS, HS-SPME-GC-MS, and UPLC-Q-Orbitrap-MS combined with chemometrics].

The study investigated the intrinsic changes in material basis of Angelicae Sinensis Radix during wine processing by headspace-gas chromatography-ion mobility spectrometry(HS-GC-IMS), headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS), and ultra-high performance liquid chromatography-quadrupole-orbitrap mass spectrometry(UPLC-Q-Orbitrap-MS) combined with chemometrics. HS-GC-IMS fingerprints of Angelicae Sinensis Radix before and after wine processing were established to analyze the variation trends of volatile components and characterize volatile small-molecule substances before and after processing. Principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA) were employed for differentiation and difference analysis.

Gut microbial signatures in type 2 diabetes are highly associated with geographic region, diet habits and common comorbidities: Insights from a bioinformatics analysis of Chinese population.

Aims: Emerging evidence implicates the gut microbiota in type 2 diabetes (T2D) development and their association with multiple host factors. This study aimed to explore the differences in gut microbiota between T2D patients and non-diabetic controls, and to estimate the relationship between host factors and specific microbial signatures in T2D.

Methods: 16S rRNA gene sequencing was performed on fecal samples from 508 T2D patients and 1,538 controls in a Chinese population.

Pancancer outcome prediction via a unified weakly supervised deep learning model.

Accurate prognosis prediction is essential for guiding cancer treatment and improving patient outcomes. While recent studies have demonstrated the potential of histopathological images in survival analysis, existing models are typically developed in a cancer-specific manner, lack extensive external validation, and often rely on molecular data that are not routinely available in clinical practice. To address these limitations, we present PROGPATH, a unified model capable of integrating histopathological image features with routinely collected clinical variables to achieve pancancer prognosis prediction.

The associations between prenatal rare earth elements exposure and preterm birth: integrate insights from birth cohort and in vitro study.

Rare earth elements (REEs) are emerging environmental contaminants, yet their effects on preterm birth (PTB) remain poorly understood. This study integrates epidemiological and mechanistic evidence to evaluate REEs-associated PTB risk and potential biological pathways. We analyzed 4,897 mother-child pairs from a large cohort in China and 13 REEs in maternal urine were measured.

A novel polysaccharide in Polygonatum kingianum: structure elucidation, the activities of anti-inflammatory and the regulation of gut microbiota in vitro.

Polysaccharides are the primary active constituents of Polygonatum kingianum Coll. et Hemsl. However, the comprehensive characterization of P.

Communicating with two vehicles immediately ahead boosts traffic capacity sixfold in connected autonomous vehicle platoons.

Addressing urban congestion through enhanced traffic capacity has emerged as a critical objective for connected autonomous driving technologies. An irredundant communication connectivity topology is essential for ensuring the high efficiency and stability of the traffic system, which has not been fully validated due to the scarcity of real-world tests. Motivated by this fact, this paper deploys a connected autonomous vehicle platoon without relying on the information of a platoon leader to preserve the possibility of extending the platoon in future practical applications.

Regulatory Effects of Alkali-Extracted Polysaccharide Induced Intestinal Enrichment on Peripheral Blood Proteomics in Tumor-Bearing Mice.

polysaccharides have demonstrated multiple biological activities including immune regulation, antitumor, and antioxidant properties. The rapid development and integrated application of multi-omics can facilitate the unraveling of the complex network of immune system regulation. In this study, alkali-extracted polysaccharide (CPAP) were prepared, and their effects on gut microbiota compositions, metabolic pathways, and protein expressions in peripheral blood and solid tumors in mice were further evaluated.

Engineering noncovalent π-stacked organic framework for intrinsic near-infrared photoactivated drug delivery.

Photoactivated drug delivery is a promising therapeutic strategy that enables spatial and temporal control of payload release. A critical component of this approach is the photoresponsive material that has sufficient drug-loading capacity and can be actuated by near-infrared (NIR) light with considerable penetration depths. Here, we establish a photoactivated drug delivery platform based on the π-stacked organic framework (πOF), which demonstrates an intrinsic NIR absorption and superior photothermal effect.

Screening of high -glucosidase-producing yeast strains from the Penglai wine region (China) and their fermentation performances and aroma compositions in Petit Manseng wine fermentation.

The majority of aroma precursors in grapes exist as odorless glycosidic conjugates, which can be hydrolyzed by -glucosidase to release free volatile aroma compounds, thereby enhancing the aromatic quality of wine. This study aimed to screen and characterize indigenous non- strains with -glucosidase activity for their potential to enhance terpenoid aroma compounds during wine fermentation. Grapes collected from 14 vineyard plots in the Penglai Wine Region (China) underwent spontaneous fermentation and yielded 203 single colonies.

Efficient and selective leaching of nickel and cobalt from nickel laterite ore by a combined atmospheric acid leaching-ferric chloride solution leaching process: optimization and mechanism.

Low-selectivity leaching of nickel and/or cobalt is a major problem for atmospheric acid leaching processes, while harsh leaching conditions make the pressurised acid leaching process costly. Thus, the development of an efficient and selective leaching process of cobalt and nickel under mild conditions is required. Here, a combined atmospheric acid leaching-ferric chloride solution leaching process was developed to leach cobalt and nickel selectively and efficiently over iron from nickeliferous laterite ores.

Whole Genome Sequencing Reveals How Plasticity and Genetic Differentiation Underlie Sympatric Morphs of Arctic Charr.

Salmonids have a remarkable ability to form sympatric morphs after postglacial colonisation of freshwater lakes. These morphs often differ in morphology, feeding and spawning behaviour. Here, we explored the genetic basis of morph differentiation in Arctic charr (n = 283) by first establishing a high-quality reference genome and then using this in whole genome sequencing of distinct morphs present in two Norwegian and two Icelandic lakes.

Atomic-scale investigation of the role of supports in nanocrystal growth.

Metal nanoparticles supported on solid materials form the cornerstone of industrial catalysis, where metal-support interactions play a critical role in tuning the chemistry of active sites. Beyond their influence post-synthesis, supports exert a profound impact during the initial stages of nanocrystal growth, significantly affecting the catalytic performance. However, a detailed atomic-level understanding of these processes remains elusive.

FAP deficiency attenuates T2DM-associated HFpEF by suppressing the CaMKIIδ-Calcineurin A-NFATc2 signaling pathway.

Heart failure with preserved ejection fraction (HFpEF) represents the initial phase of cardiac dysfunction associated with type 2 diabetes mellitus (T2DM). To date, the pathophysiological mechanisms underlying T2DM-induced HFpEF are complex and elusive. Fibroblast activation protein (FAP) is a prolyl-specific serine protease that its inhibition or vaccination has been shown to enhance cardiac repair following myocardial infarction (MI).

Targeting of the CD161 Inhibitory Receptor Enhances Bone-Marrow-Resident Memory CD8 T-Cell-Mediated Immunity against Multiple Myeloma.

Immune checkpoint inhibitors (ICIs) have transformed the treatment of many solid tumors. Still, their effectiveness in multiple myeloma (MM) remains underwhelming, highlighting the need to explore alternative approaches beyond conventional ICIs. Here, CD161 is identified as a novel inhibitory receptor on bone marrow (BM) tissue resident memory CD8 T cells (CD8 TRM), known for their sustained presence and vital role in local immune surveillance in MM BM tumor microenvironments.

ZnO-NPs Neurotoxicity to : Disorders of Fatty Acid Synthesis and Glutathione Metabolism.

Zinc oxide nanoparticles (ZnO-NPs) are a widely used class of engineered nanomaterials, but their increasing environmental release raises concerns as potential neurotoxic contaminants. Although ZnO-NPs are known to induce cognitive deficits and motor dysfunction in organisms, their underlying molecular mechanisms remain unclear. This study systematically investigated ZnO-NPs-induced neurotoxicity in , revealing significant perturbations in lipid homeostasis, concomitant with impaired foraging behavior, learning and memory deficits, and chemotaxis dysfunction, along with neuronal damage.

Root-to-shoot mobile mRNA CmoKARI1 promotes JA-Ile biosynthesis to confer chilling tolerance in grafted cucumbers.

Pumpkin rootstock is commonly used to graft cucurbit crops, improving their ability to withstand stress. While the significance of systemic signals from rootstocks to scions is recognized, the role of root-to-shoot transported mRNAs remains understudied. Cucumber plants often face growth and productivity limitations due to low temperatures.

Collagen alpha-5(IV) chain activation by nuclear factor 1/C promotes nasopharyngeal carcinoma progression.

The expression of collagen receptors by cancer cells serves a vital function in the regulation of cell behavior. These receptors are capable of sensing the signals generated by alterations in the collagen state, thereby contributing to the maintenance of cellular homeostasis. The discoidin domain receptor (DDR)1 functions as a critical sensor of collagen fiber state and composition, regulating tumor cell growth, response to therapy, and patient survival.

[The function of circular RNA-microRNA-messenger RNA immune regulatory network in childhood allergic asthma].

Objectives: To investigate the potential circular RNA (circRNA)-microRNA (miRNA)-messenger RNA (mRNA) immune regulatory network in childhood allergic asthma by analyzing microarray datasets.

Methods: GEO database was used to obtain the datasets of circRNA, miRNA, and mRNA from children with allergic asthma and healthy controls. The Limma package was used to identify differentially expressed circRNA (DEcircRNA), miRNA (DEmiRNA), and mRNA (DEmRNA).

A crosslinked poly(vinyl alcohol) core-shell microneedle patch for complete and controlled insulin delivery and sustained hypoglycemic therapy.

Complete and controlled drug delivery has long been a key objective in microneedle technology. Here, we designed a novel powder-loaded crosslinked poly(vinyl alcohol) microneedle patch featuring a controlled-release shell and drug-propelling core structure, designed for the complete and controlled high-dose delivery of insulin and sustained hypoglycemic therapy. The direct encapsulation of insulin in solid form significantly enhanced the drug loading capacity, particularly of poorly soluble drugs, and minimized degradation.

4-Hydroxychalcone alleviated Angiotensin II-induced atrial fibrillation via immunoproteasome-IKK-NF-κB signaling pathway modulation.

Atrial Fibrillation (AF) is the most common type of cardiac arrhythmia and a significant contributor to stroke occurrence. Although 4-Hydroxychalcone (4HCH) has notable anti-inflammatory and antioxidant properties, playing critical therapeutic roles in hypertensive nephropathy and cardiac remodeling, its effects on AF are somewhat unclear, thus forming the basis of this study. Herein, AF was induced in mice via continuous infusion of Angiotensin II (Ang II) at a dose of 2000 ng/kg/min for three weeks.

Succinate preserves CD8 T cell fitness to augment antitumor immunity.

Succinate, a tricarboxylic acid cycle intermediate, accumulates in tumors with succinate dehydrogenase (SDH) mutations. Although succinate is recognized for modulating CD8 T cell cytotoxicity, its impact on T cell differentiation remains poorly understood. Here, we reveal that succinate accumulation in tumors lacking the SDH subunit B (SDHB) enhanced tumor-reactive CD8 T cell-mediated immune responses.