Genome-Wide Simplification of the AcMNPV Genome Using Synthetic Biology.

Large-scale genome simplification represents a fundamental goal in synthetic biology. Baculoviruses, with their biphasic life cycle and inherent genomic plasticity, have emerged as ideal models for synthetic genome engineering. Although modified baculovirus genomes are widely used as expression vectors for robust recombinant protein production, many genomic regions are dispensable for in vitro budded virus (BV) production.

RNF126 suppresses amino acid-mediated mTORC1 signaling pathway by ubiquitinating ILF3 in HEK293T cells.

The mammalian Target of Rapamycin Complex 1 (mTORC1) serves as a metabolic hub that integrates external nutrients to promote cell growth and metabolism, with its activation closely associated with accelerated cancer progression. Interleukin enhancer-binding factor 3 (ILF3) has been identified as a negative regulator of mTORC1 by tethering GATOR1/2 to the lysosomal membrane during amino acid sensing. However, the regulatory mechanisms of the ILF3-mediated mTORC1 signaling pathway remain unclear.

2-1,4-Benzoxazin-3(4)-one linked 1,2,3-triazole derivatives and their study on inducing DNA damage in tumor cells.

This study explores the anticancer potential of rigid planar compounds capable of intercalating into tumor cell DNA, thereby inducing DNA damage and subsequent cell death. A novel series of compounds () were synthesized using 2-1,4-benzoxazin-3(4)-one as the planar core structure, with 1,2,3-triazole groups introduced at the 7-position. Biological evaluation across multiple human tumor cell lines revealed that several c-series compounds exhibited notable inhibitory activity against Huh-7 liver cancer cells, with IC values of 28.

Tenascin-R aggravates Aβ production in the perforant pathway by regulating Nav1.6 activity in APP/PS1 mice.

Introduction: Alzheimer's disease (AD) neuropathology exhibits early accumulation of amyloid beta (Aβ) plaques within the perforant pathway. This study explores how tenascin-R, a myelin-associated protein at nodes of Ranvier (NORs), modulates Aβ generation through Nav1.6 within this cortico-hippocampal circuit.

Probing stress and magnetism at high pressures with two-dimensional quantum sensors.

Pressure serves as a fundamental tuning parameter capable of drastically modifying all properties of matter. The advent of diamond anvil cells (DACs) has enabled a compact and tabletop platform for generating extreme pressure conditions in laboratory settings. However, the limited spatial dimensions and ultrahigh pressures within these environments present significant challenges for conventional spectroscopy techniques.

The Immunogenicity of Glutaraldehyde Inactivated PTx Is Determined by the Quantity of Neutralizing Epitopes.

Background/objectives: Chemically or genetically detoxified pertussis toxin (PTx) is a crucial antigen component of the acellular pertussis vaccine. Chemical detoxification using glutaraldehyde generally causes significant structural changes to the toxin. However, how these structural changes in PTx affect its antigenic properties remains unclear.

Fixed-Time Active Disturbance Rejection Temperature-Pressure Decoupling Control for a High-Flow Air Intake System.

High-flow aeroengine transient tests involve strong coupling and external disturbances, which pose significant challenges for intake environment simulation systems (IESSs). This study proposes a compound control scheme that combines fixed-time active disturbance rejection with static decoupling methods. The scheme integrates a fixed-time sliding-mode controller (FT-SMC) and a super-twisting fixed-time extended-state observer (ST-FT-ESO).

The Prediction Model for Triple-Negative Breast Cancer Prognosis and Immunotherapy Efficacy Based on Single-Cell Sequencing of CD8+ T cells.

Triple-negative breast cancer (TNBC) exhibits a higher propensity for recurrence, distant metastasis, and mortality than the other subtypes of breast cancer. TNBC is primarily attributed to the lack of expression of the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). Single-cell sequencing results of CD8 T cells in TNBC patients were screened for differentially expressed and immune-related genes.

Exploring the multifaceted roles of glutamate oxaloacetate transaminase 1 as a biomarker and therapeutic target in colorectal cancer and pan-cancer analyses.

Colorectal cancer (CRC) is a global health issue requiring novel diagnostic and therapeutic approaches to improve patient outcomes. Glutamate oxaloacetate transaminase 1 (GOT1) plays a crucial role in metabolism and is associated with various cancers. However, its expression and potential as a diagnostic marker in CRC have not been thoroughly investigated.

Quantitative assessment of the associations between MTR and MTRR gene polymorphisms and glioma risk.

Purpose: Previous research on the correlation between methionine synthase (MTR) and methionine synthase reductase (MTRR) gene polymorphisms and the susceptibility to glioma has yielded varied results. This study aims to elucidate the potential impact of MTR and MTRR polymorphisms as contributing factors in the development of glioma.

Patients And Methods: A comprehensive review of the relevant literature was conducted across several major databases, encompassing records from their inception through April 2025.

Multi-Target Repetitive Transcranial Magnetic Stimulation Improves Freezing of Gait in Parkinson's Disease: A Randomized Controlled Trial.

Objective: In this randomized, double-blind, sham-controlled trial, we investigated the efficacy of multi-target 10-Hz rTMS targeting both M1 and SMA in alleviating freezing of gait (FOG) in Parkinson's disease (PD) patients.

Methods: Eighty-four PD-FOG patients were randomly assigned (1:1:1:1) to four groups: M1 and SMA group, M1 group, SMA group, and sham group. rTMS was administered once daily for 10 consecutive days.

Synthesis of 2-Trifluoromethylindolines via Rh(III)-Catalyzed Chelation-Assisted C-H Bond Activation and Annulation of Anilines with CF-Imidoyl Sulfoxonium Ylides.

Rh(III)-catalyzed C-H bond activation and annulation of -pyrimidyl-substituted anilines with CF-imidoyl sulfoxonium ylides (TFISYs) have been disclosed, affording a variety of 2-trifluoromethylindolines that contain a quaternary carbon center. A cascade C-H imidoylmethylation, tautomerization, and intramolecular nucleophilic addition sequence is involved in the transformation. The reaction could be scaled up to 2 mmol.

Safety monitoring of health outcomes following influenza vaccination during the 2023-2024 season among U.S. Commercially-insured individuals aged 6 months through 64 years: Self-controlled case series analyses.

Background: Influenza vaccines are reformulated annually to target expected virus strains for the upcoming season. Although the safety of seasonal influenza vaccines is well-established, active safety surveillance of reformulated vaccines is essential to assess occurrence of potential health outcomes among vaccinated individuals. This study evaluated the safety of the 2023-2024 influenza vaccines among U.

Causal Effect of Plasma Fatty Acid Profiles on Psoriasis Risk: Genetic Evidence from a Mendelian Randomization Study.

Introduction: Emerging evidence indicates that omega-3 fatty acids from fish oil may serve as beneficial dietary supplements for psoriasis management. Clinical observations demonstrate a significant association between psoriasis improvement and increased docosahexaenoic acid (DHA) levels. However, the causal relationship between fatty acids and psoriasis risk requires further investigation.

Characterization of key astringent compounds and optimization of the fixation process of early tea in Chuanyu region.

Due to inadequate fixation, early tea produced in the Chuanyu region exhibits high astringency. To mitigate the astringency of early tea, this study identified the astringent compounds present in the tea, subsequently optimized its fixation process based on the key astringent substances. Initially, 20 early tea samples were categorized into three groups (low, middle, and high astringency) according to the differences in astringency.

Bacterial dysentery complicated with Crohn's disease: A case report.

Rationale: Crohn's disease is easily confused with bacillary dysentery in clinical manifestations. Crohn's disease is a chronic disease that can involve both intestinal and extraintestinal areas, and severe patients have prolonged and poor prognosis, so early diagnosis and treatment are crucial for patients. This case is a combination of bacillary dysentery and Crohn's disease, which emphasizes the differentiation between bacillary dysentery and Crohn's disease, and expects clinicians to pay attention to and discover the early manifestations of Crohn's disease.

Automated Extraction of Mortality Information From Publicly Available Sources Using Large Language Models: Development and Evaluation Study.

Background: Mortality is a critical variable in health care research, especially for evaluating medical product safety and effectiveness. However, inconsistencies in the availability and timeliness of death date and cause of death (CoD) information present significant challenges. Conventional sources such as the National Death Index and electronic health records often experience data lags, missing fields, or incomplete coverage, limiting their utility in time-sensitive or large-scale studies.

Effects of non-thermal processing techniques on cereal bran: Focus on changes in bran structure and polyphenol release.

Cereal bran, a nutrient-rich by-product of grain processing, contains high levels of dietary fiber, polyphenols, and bioactive compounds, offering antioxidant and anti-inflammatory benefits. However, its rigid cell wall structure limits the release of bioactive compounds, requiring modification for food applications. Conventional thermal processing degrades heat-sensitive components and reduces polyphenol yields, whereas non-thermal methods (physical, chemical, and biological) better preserve and enhance the release of bioactive compounds.

Optimizing processing methods for maximum bioactive retention: comparative metabolomic analysis of dried loquat () flowers and their powdered extracts.

Loquat () flowers are a rich source of bioactive flavonoids, but their nutraceutical potential depends on post-harvest processing. This study evaluated the impact of heat-drying (HD) and freeze-drying (FD) on flavonoid retention in loquat flowers and their hot-water powdered extracts using UPLC-MS/MS metabolomics and antioxidant assays. Freeze-drying significantly preserved thermolabile compounds, with cyanidin showing a 6.

Tea Polyphenol Modulates Mitochondria-Associated Endoplasmic Reticulum Membrane of Hippocampal Neurons Targeting Grp75 to Ameliorate Memory Impairment in the Aged T2DM Rats.

Tea polyphenol (TP), as the most abundant and unique functional substance of tea, has been widely studied for the neuroprotective effects. Previous studies also found that TP improves the memory impairment of the aged T2DM rats, but the underlying molecular mechanism has not been fully clarified. The model of aged T2DM was induced by injecting D-galactose and STZ intraperitoneally, as well as feeding with a high-glucose-fat diet in rats, and D-galactose and D-glucose were used in PC12 cells.

Effects of supplementation with krill oil on blood parameters, hair quality, and fecal microbiota in male beagle dogs.

Krill oil (KO) is a bioactive substance with antioxidant and immunomodulatory functions. This study investigated the impact of administering snacks supplemented with 0.5% KO for 8 weeks on the blood parameters, hair quality, and fecal microbiota in dogs.

Engineering Bienzymatic Cascade for Efficient Biosynthesis of Citronellal and Citronellol.

Citronellal and citronellol are monoterpenoids that serve as key ingredients in the flavor and fragrance industry and hold significant potential for pharmaceutical applications. Currently, these compounds are primarily obtained through chemical synthesis or extraction from plant essential oils. However, these methods raise concerns regarding environmental sustainability, carbon emissions, and the growing attentions for natural production.

Single-cell profiling identifies biomarkers for immunochemotherapy in esophageal squamous cell carcinoma.

Esophageal squamous cell carcinoma (ESCC) remains a highly aggressive malignancy with limited therapeutic options. While immune checkpoint blockade (ICB) combined with chemotherapy has improved outcomes, resistance mechanisms and predictive biomarkers are poorly understood. This study aims to characterize the dynamic changes in the tumor microenvironment (TME) during ICB treatment and identify cell populations and molecular programs associated with therapeutic response.