Optimizing processing methods for maximum bioactive retention: comparative metabolomic analysis of dried loquat () flowers and their powdered extracts.

Loquat () flowers are a rich source of bioactive flavonoids, but their nutraceutical potential depends on post-harvest processing. This study evaluated the impact of heat-drying (HD) and freeze-drying (FD) on flavonoid retention in loquat flowers and their hot-water powdered extracts using UPLC-MS/MS metabolomics and antioxidant assays. Freeze-drying significantly preserved thermolabile compounds, with cyanidin showing a 6.

Neuroprotective effects of Tongmai Yizhi Decoction () against Alzheimer's disease through attenuating cyclin-dependent kinase-5 expression.

Objectives: To explore the protective effects of Tongmai Yizhi Decoction (, TYD), a Chinese herb complex prescription against the impairment of cognitive functions and memory loss in amyloid beta 1-40 (Aβ) peptide and ibotenic (IBO)-induced Alzheimer's disease (AD) model rats.

Methods: The in vivo model was established by injecting Aβ and IBO into left hippocampal CA1 area of Sprague-Dawley (SD) rat to mimic AD. Totally 32 SD rats were divided into 4 groups, including sham operation group, AD model group, TYD group [AD rats treated with TYD at the dosage of 19.

Peroxynitrite Decomposition Catalyst Reduces Delayed Thrombolysis-induced Hemorrhagic Transformation in Ischemia-reperfused Rat Brains.

Aim: Hemorrhagic transformation (HT) is a major complication of delayed tissue plasminogen activator (t-PA) treatment in ischemic stroke. We aimed to explore whether peroxynitrite decomposition catalyst (PDC) could prevent such complication.

Methods: Male Sprague-Dawley (SD) rats were subjected to middle cerebral artery occlusion (MCAO) with t-PA (10 mg/kg) or t-PA plus FeTMPyP (3 mg/kg, a representative PDC) at MCAO for 2 or 5 h and reperfusion for 22 or 19 h, respectively.