Membrane contact sites between chloroplasts and the pathogen interface underpin plant focal immune responses.

Communication between cellular organelles is essential for mounting effective innate immune responses. The transport of organelles to pathogen penetration sites and their assembly around the host membrane, which delineates the plant-pathogen interface, are well-documented. However, whether organelles associate with these specialized interfaces, and the extent to which this process contributes to immunity, remain unknown.

Effect of LPJZ-658 on caecum microbiota and serum metabolomics of Luhua broiler.

Introduction: The purpose of this study was to investigate the effects of dietary supplementation of LPJZ-658 on body weight and serum indexes of Luhua broiler, and to explore the relevant mechanism of probiotic function of LPJZ-658 based on intestinal microbiota and serum metabolomics.

Methods: One hundred one-day-old Luhua broiler were randomly divided into the control group (CON) and LPJZ-658 treatment group (LPJZ-658). The CON group was fed a basal diet, and the LPJZ-658 group was fed the basal diet supplemented with 2 × 10 cfu/kg of LPJZ-658.

Anisotropic Optoelectronic Synapses in 2D NbGeTe for Direction-Programmable Neuromorphic Perception and Decision-Making.

Neuromorphic computing presents a promising solution for the von Neumann bottleneck, enabling energy-efficient and intelligent sensing platforms. Although 2D materials are ideal for bioinspired neuromorphic devices, achieving multifunctional synaptic operations with simple configurations and linear weight updates remains challenging. Inspired by biological axons, the in-plane anisotropy of 2D NbGeTe is exploited to develop dual electronic-optical synaptic devices.

Probing stress and magnetism at high pressures with two-dimensional quantum sensors.

Pressure serves as a fundamental tuning parameter capable of drastically modifying all properties of matter. The advent of diamond anvil cells (DACs) has enabled a compact and tabletop platform for generating extreme pressure conditions in laboratory settings. However, the limited spatial dimensions and ultrahigh pressures within these environments present significant challenges for conventional spectroscopy techniques.

Organic Fusion of Molecular Simulation and Wet-Lab Validation: A Promising High-Throughput Strategy for Screening Bioactive Food Peptides.

Peptides derived from protein sources in food exhibit a diverse array of biological activities. The screening, preparation, and functional investigation of bioactive peptides have become a focal area of research. This review summarizes the status of peptide activity mining, including the latest research progress in protein sources, peptide functions, and processing conditions.

Regional citrate anticoagulation for intermittent renal replacement therapy in critically ill patients: a retrospective case-control study.

Background: Regional citrate anticoagulation (RCA) is gradually adopted for intermittent kidney replacement therapy (IRRT) in critically ill patients to mitigate circuit clotting. However, evidence comparing its efficacy and safety remains limited. This study aimed to (1) validate the safety and efficacy of regional citrate anticoagulation (RCA) compared to conventional anticoagulation avoidance during intermittent renal replacement therapy (IRRT) in a critical care nephrology cohort, and (2) establish practical criteria for selecting RCA protocols based on individualized patient bleeding and clotting risk assessments.

Plasma-activated water treatment delayed the lipid oxidation and degradation of golden pompano fillets during storage based on lipidomics.

Lipid oxidation and degradation during storage represent key challenges affecting the quality of golden pompano () fillets. This study aimed to investigate the dynamic changes in lipid profiles of plasma-activated water (PAW)-treated fillets over 18 days, using chemical analysis and UHPLC-MS-based lipidomics. The results showed that the PAW-treated group exhibited higher POV and TBARS values within 0-6 days, but significantly delayed their increase within 6-18 days.

An Alternative Approach to Acquiring Permeability from Ultrasonic S-Wave.

Water interaction between fractures and rock matrix is one of the themes in hydrogeology. Accurate values of Darcy permeability (k) of the matrix are desired for better quantification of the water interaction. In contrast to the traditional method using seepage experiments to measure k of a rock, this study uses the technique of ultrasonic shear (S-) wave for determining k of the rock matrix.

Pediatric endocardial temporary pacemaker implantation: Clinical characteristics and outcomes from a Chinese National Regional Health Center.

Endocardial temporary pacemakers (TP) are widely used in the treatment of cardiovascular diseases in adults, but their application and clinical experience in the treatment of pediatric cardiovascular cases are relatively limited. This study aims to share the experience of using endocardial TP at a pediatric medical center in China. The study included patients who received their first endocardial TP implantation in the Department of Cardiology at Children's Hospital of Chongqing Medical University, China, from 2016 to 2022.

Sphingoid bases extracted from Hippocampus trimaculatus Leach induces apoptosis in human leukemia K562 cells through the mitochondria-dependent pathway.

Ethnopharmacological Relevance: Hippocampus trimaculatus is an important traditional Chinese medicine, which has been used to warm and tonify kidney yang in the clinic for a long time. Modern studies have also shown that it can also display various pharmacological activities such as anti-inflammation and antitumor due to the abundant ingredients. However, its therapeutic effect and mechanism against tumors like leukemia, which emerge as a prevalent type of hematological tumor disease, requires further research.

Insights into the construction of binary protein systems and exploration of gel properties: Effect of bee pupa powder on the gel properties of myofibrillar protein from Litopenaeus vannamei.

This investigation elucidated the effects of bee pupa powder (BPP) incorporation on the gelation behavior of Litopenaeus vannamei myofibrillar protein (MP), utilizing advanced microstructural analyses and molecular interaction assessments. Laser confocal microscopy revealed a homogeneous dispersion of BPP within the surimi matrix, with optimal BPP: MP ratios (1:20, w/w) facilitating the unfolding of tertiary protein structures through enhanced hydrophobic interactions and disulfide bond formation, thereby reinforcing the three-dimensional gel network. Beyond this threshold, steric hindrance effects predominated, diminishing interfacial interactions and gel integrity.

Advances of nano-photofertilizers in plant research.

There is a saying that all living things grow by the sun, which signifies the vital role of solar energy in the accumulation of biochemical energy on the earth. As primary producers in the ecosystem, plants play fundamental roles in bioaccumulation. With the rapid advancement of nanotechnology, nanomaterials (NMs) have been playing increasingly essential roles in the field of plant research.

Effects of Coconut Exocarp Flavonoid and EDTA-2Na on Aldehyde Generation During Pan-Frying Processing of Squid ().

Squid is rich in polyunsaturated fatty acids (PUFAs), especially docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), which exert various human health benefits. Pan-fried squid is a popular processed product beloved by consumers. However, the PUFAs of squid can be severely oxidized during thermal processing, which will result in the reduction in nutritional value and generation of harmful compounds like aldehydes.

BpWRKY6 regulates insect resistance by affecting jasmonic acid and terpenoid synthesis in Betula platyphylla.

Forest pests and diseases pose serious threats to the sustainable development of forestry. Plants have developed effective resistance mechanisms through long-term evolution. Jasmonic acid and terpenoids play important roles in the defence response of plants against insects.

Pushing Photodetection Beyond the Limit of Silicon PIN Junctions Through In Situ Integration of Randomly Interlinked Gold Nanoparticles and Black Silicon.

High-responsivity and broad-spectrum photodetectors are indispensable for advanced photoelectric applications. While silicon PIN junctions offer several advantages, such as mature manufacturing processes, stable performance, and cost-effectiveness, their near-infrared detection capabilities are fundamentally constrained by the intrinsic properties of silicon. In this study, we propose a device structure that advances photodetection beyond the limitation of silicon PIN junctions by in situ integrating randomly interlinked gold nanoparticles and black silicon (RIL-AuNPs/B-Si).

Construction of gas content model based on KPCA-SVR for Southern Sichuan shale gas.

The gas content characteristics in shale gas reservoirs, including volume and distribution, are critical for optimizing extraction and resource utilization. However, the relationship between gas content (Vg) and well logging parameters (e.g.

Van der Waals Antiferroelectric CuCrPS-Based Artificial Synapse for High-Precision Neuromorphic Computation.

2D van der Waals heterostructure-based artificial synapses have emerged as a compelling platform for next-generation neuromorphic systems, owing to their tunable electrical conductivity and layer-engineered functionality through controlled stacking of 2D materials. In this work, an engineered SnS₂/h-BN/CuCrP₂S₆ van der Waals antiferroelectric field-effect transistor (AFe-FET) is presented that implements synaptic weight modulation through the synergistic interplay of charge trapping dynamics and electric-field-controlled ferroelectric polarization switching. The AFe-FET architecture successfully emulates essential neuroplasticity features, including paired-pulse facilitation, short-term plasticity, and long-term plasticity.

[Recombinant expression of esterase SyEst870 capable of degrading carbamate pesticides].

Carbamate pesticides, a new type of broad-spectrum pesticides for controlling pests, mites, and weeds, are developed to address the shortcomings of organochlorine and organophosphorus pesticides. Their widespread use and slow degradation have led to environmental pollution, causing damage to ecosystems and human health. Managing pesticide residues is a pressing issue in the current environmental protection.

Modulation of Magnetic Domain and the Occurrence of Antisymmetric Magnetoresistance in the Folded van der Waals Ferromagnet FeGaTe.

Emergent magnetism in 2D materials has attracted significant attention due to their intrinsic magnetic order, which persists down to the monolayer limit, and their potential applications in spintronic devices. In particular, domain structure modulation plays a crucial role in 2D magnet-based nano-spintronic devices. However, the fabrication and modulation of the desired domain structure, along with the establishment of reliable electrical write/read operations, remain significant challenges.

High-Photon-Harvesting Nanophotofertilizers for Plant Growth Multiregulation.

Nanophotoresponsive technology has emerged as a promising way to enhance plant photosynthesis, but it faces limitations in light absorption and electron transfer efficiency. This study presents a photoresponsive nanosystem, LDNPs@Fe,Cu-CDs, combining lanthanide-doped nanoparticles (LDNPs) and Fe/Cu dual single-atom-doped carbon dots (CDs). Fabricated via hydrothermal synthesis, the nanosystem can regulate plant growth through light absorption, photothermal effects, photoelectron generation, and photocatalysis.

Reversible shuffle twinning yields anisotropic tensile superelasticity in ceramic GeSe.

Superelasticity is a reversible, nonlinear strain response to stress stimuli beyond the linear elastic regime. It is commonly associated with a martensitic transformation in its host material, usually a metal or polymer. Except for the ceramic crystals ZrO and BaTiO, which show superelasticity under compressive stress, inorganic materials with covalent or ionic bonding usually do not exhibit superelastic behaviour because of large energy barriers for structural transitions.

Nat10-mediated N4-acetylcytidine modification enhances Nfatc1 translation to exacerbate osteoclastogenesis in postmenopausal osteoporosis.

Increased differentiation or activity of osteoclasts is the key pathogenic factor of postmenopausal osteoporosis (PMOP). N4-acetylcytidine (ac4C) modification, catalyzed by Nat10, is a novel posttranscriptional mRNA modification related to many diseases. However, its impact on regulating osteoclast activation in PMOP remains uncertain.

TiC/CuWO/Pt nanozyme: photothermal-enhanced chemodynamic antibacterial effects induced by NIR.

With the growing issue of antibiotic resistance, it has become increasingly crucial to develop highly efficient antimicrobial materials. While the single-component nanozyme systems exhibited some catalytic activity, their efficiency remains suboptimal. This study presents a TiC/CuWO/Pt hybrid nanozyme composed of photothermal agents and nanozymes, which leverages the photothermal effect to enhance nanozyme activity and achieve efficient antimicrobial effects.

BpMAPK3-mediated BpWRKY53 phosphorylation enhances Betula platyphylla drought stress tolerance by increasing flavonoid content.

The increasing intensity, frequency, and duration of drought pose a threat to the survival of some tree species worldwide, directly damaging the carbon sequestration capacity of forest ecosystems. Understanding the response mechanisms of trees to drought stress is particularly important. In this study, a drought stress regulatory network in Betula platyphylla (birch) was established by observing the changes in the root transcriptome at different drought stress time points (0, 3, 6, 24, 48, and 72 h), and a potential drought-resistant WRKY53 transcription factor was identified.

Utilization of layer-by-layer deposition to improve the stability of astaxanthin emulsions: Triple-layer coatings formed using sodium caseinate-pectin-chitosan.

This study aimed to develop a good multilayered emulsion delivery system to improve the stability of astaxanthin. The layer-by-layer (LBL) electrostatic deposition technique was utilized to prepare sodium caseinate-pectin-chitosan astaxanthin multilayered emulsions. The stabilities of the emulsions and astaxanthin under different environmental stresses were investigated.