Green ultrasound‑assisted deep eutectic solvent extraction of flavonoid enzyme inhibitors from : process optimization, characterization, and mechanistic insights into ‑glucosidase and tyrosinase inhibition.

A green ultrasound-assisted deep eutectic solvent (UAEDES) method was optimised for extracting flavonoid enzyme inhibitors from . Optimal conditions (choline chloride-1,4-butanediol 1:3 molar ratio, 43% water content, 50 mL/g liquid-to-solid ratio, 80 °C ultrasound for 48 min) yielded 3.15% total flavonoids, 45.

Climate-adaptive energy forecasting in green buildings via attention-enhanced Seq2Seq transfer learning.

Energy consumption forecasting in green buildings remains challenging due to complex climate-building interactions and temporal dependencies in energy usage patterns. Existing prediction models often fail to capture long-term dependencies and adapt to diverse climatic conditions, limiting their practical applicability. This study presents an integrated forecasting framework that combines sequence-to-sequence (Seq2Seq) architecture with reinforcement learning and transfer learning techniques.

An Analysis of the Different Salt-Tolerance Mechanisms in Rice Cultivars Induced by Cerium Oxide Nanoparticles.

Cerium oxide nanoparticles (CeONPs) can boost crops' salt tolerance, yet their regulatory mechanisms in rice cultivars with contrasting salt tolerance remain unclear. This study investigated the regulatory differences in poly (acrylic acid)-coated nanoceria (PNC)-primed in salt-sensitive (Huanghuazhan, H) and salt-tolerant (Xiangliangyou900, X) rice. The results showed that PNC priming improved salt tolerance in two cultivars, but the underlying mechanisms differed.

Age-Stratified Efficacy of Upadacitinib in Refractory Pediatric Crohn's Disease and Geriatric Ulcerative Colitis: An Asian Cohort Study.

Background And Aims: Inflammatory Bowel Disease presents significant management challenges, particularly in pediatric and elderly populations with refractory conditions. Despite advances in biologic therapies, current treatments remain limited with inconsistent response rates. This study investigated Upadacitinib efficacy in Asian adolescent and elderly populations with refractory IBD.

Regional citrate anticoagulation for intermittent renal replacement therapy in critically ill patients: a retrospective case-control study.

Background: Regional citrate anticoagulation (RCA) is gradually adopted for intermittent kidney replacement therapy (IRRT) in critically ill patients to mitigate circuit clotting. However, evidence comparing its efficacy and safety remains limited. This study aimed to (1) validate the safety and efficacy of regional citrate anticoagulation (RCA) compared to conventional anticoagulation avoidance during intermittent renal replacement therapy (IRRT) in a critical care nephrology cohort, and (2) establish practical criteria for selecting RCA protocols based on individualized patient bleeding and clotting risk assessments.

Glycosylation in kidney diseases.

Protein glycosylation is a critical post-translational modification that influences protein folding, localization, stability, and functional interactions by attaching glycans to specific sites. This process is crucial for biological functions of glycoproteins, and aberrant glycosylation can lead to genetic disorders, immune system issues, and multi-organ pathologies. Recent advancements in glycoproteomic technologies have made the study of protein glycosylation a key focus for understanding the pathogenesis of kidney diseases.

The LacI-Type Transcriptional Activator NCgl2689 Directly Regulates an Operon Involved in l-Cysteine Synthesis by the Reverse Transsulfuration Pathway in .

l-Cysteine is widely used in food, pharmaceutical, animal feed, and cosmetic industries. Microbial fermentation is an economically effective method for producing l-cysteine. However, research on the synthesis and regulation mechanism of l-cysteine through the reverse transsulfuration pathway in important amino acid producing cell factory is still limited.

Brevilin A, a novel LRRC15 inhibitor, exerts potent anti-rheumatoid arthritis effects by inhibiting the LRRC15/STAT3 signaling pathway.

Purpose: Leucine-rich repeat-containing 15 (LRRC15) is a transmembrane protein that is highly expressed in the synovium of patients with rheumatoid arthritis (RA). Brevilin A (BrA), an active compound isolated from , exerts potent anti-inflammatory effects. However, the anti-RA effect of BrA and its underlying mechanism of action of BrA have not been fully elucidated.

Contrast-enhanced ultrasound and elastography predict histopathology and recovery in acute kidney injury.

Purpose: Acute kidney injury (AKI) progression involves complex microcirculatory and structural changes poorly captured by conventional metrics. This study investigates contrast-enhanced ultrasound (CEUS) and shear wave elastography (SWE) to evaluate hemodynamic redistribution and fibrosis in AKI patients.

Methods: Prospective cohort study of 16 biopsy-confirmed tubulointerstitial (TI) nephritis patients and matched controls.

Function and Regulatory Mechanism of a Two-Component Signal Transduction System cgtRS5 in Corynebacterium glutamicum.

The putative two-component system (TCS) CgtRS5, encoded by the ncgl2572-ncgl2573 gene cluster, plays a critical role in environmental adaptation in Corynebacterium glutamicum. Comparative transcriptomic analysis between the wild-type strain and a ΔcgtRS5 mutant revealed three upregulated and 107 downregulated genes in the mutant. Among these, genes involved in iron metabolism (ncgl1959), aromatic compound degradation (ncgl2320), and drug resistance (cssR) were significantly downregulated.

Casticin activates Rheb GTPase by binding to residue Trp141 and induces tumor senescence.

Senescence is a state of irreversible growth arrest that can be induced in colorectal cancer (CRC). Developing drugs that can induce senescent CRC cells without promoting stemness can significantly improve treatment outcomes. Our study reveals a unique mechanism by which casticin (CAS) induces senescence in CRC.

Proteomics in pancreatic cancer.

Pancreatic cancer (PC), one of the most aggressive malignancies, is characterized by a dismal prognosis owing to its low early detection rates, rapid progression, frequent postoperative complications, and limited efficacy of conventional oncological therapies. The fact that most patients are diagnosed at advanced stages underscores the critical importance of early detection for the formulation of effective treatment strategies. Despite substantial research efforts, the medical community still lacks consistent and dependable biomarkers for the diagnosis, classification, and prognosis of PC, highlighting the urgent need for innovative and more efficient approaches to identify pancreatic abnormalities at early stages.

Sandwich-Model Cathode Electrolyte Interphase Facilitating All-Climate High-Voltage Nickel-Rich Cathode-Based Lithium Metal Batteries with LiBF-Based Electrolyte.

All-climate lithium metal batteries are highly needed, but remains a huge challenge in cycling life due to the existence of unstable electrode electrolyte interphases, especially with nickel-rich layered oxide cathode at high cut-off voltage. To address this question, a functional and robust sandwich-model cathode electrolyte interphase (CEI) is proposed, derived from a LiBF-based electrolyte modified with para-fluorobenzeneacetonitrile (P-FBCN) additive, to realize the stability of 4.8 V Li||LiNiCoMnO (NCM94) battery operated from -60 to 60 °C.

Pop Culture in the Classroom: Associations with Student Learning Outcomes and the Underlying Psychological Mechanisms.

As an emerging pedagogical strategy, the integration of pop culture into university classrooms has gained increasing attention. This study employs a quasi-experimental design to investigate the potential benefits and psychological mechanisms of incorporating pop culture into higher education curricula. Data were collected from 511 students across two courses and four class sections through both survey responses and in-class essay evaluations.

Wide-Temperature, High-Voltage, Flame-Retardant Electrolyte for 4.8 V Li||NCM94 Batteries.

All-climate lithium metal batteries attract great interest, but their poor safety and cycling performance hinder their applications, especially with high cut-off voltage nickel-rich layered oxide cathode. Herein, we develop a high-voltage, wide-temperature, and flame-retardant electrolyte for Li|| LiNiCoMnO (NCM94) batteries with excellent cycling performance through constructing highly Li-conductive, lithophilic gradient cathode electrolyte interphase (CEI). Such CEI exhibits a gradual decrease in C─F and a gradient increase in LiF and LiBO from the outside to the inside, which effectively inhibits structural degradation of the NCM94 cathode.

Disruption of DMD gene leads to altered calcium homeostasis and metabolic shift impacting gastric Cancer cell proliferation and migration.

Background: gene has been implicated in the progression and development of several tumors, but its specific contribution to gastric cancer (GC) has not been fully elucidated.

Methods: We validated gene expression levels in the TIMER2.0 database and human gastric cancer tissue microarrays and constructed gastric precancerous lesion mouse models and gene knockout and overexpression cell lines to investigate the role of gene in gastric cancer development.

Materialists perceive their high socioeconomic status as justice: Associations with increased political participation.

Existing research has not reached a consensus on the relationship between subjective socioeconomic status (SSS) and political participation. Thus, this study investigated the psychological variables influencing the relationship between SSS and political participation. Specifically, it explored the mediating role of perceived social justice and the moderating role of materialism value.

Impact of Sulfoxaflor on Brown Marmorated Stink Bug: Developmental and Reproductive Effects.

The brown marmorated stink bug, , has become a severe threat to agriculture worldwide with continuous invasion and spread. Sulfoxaflor is the pioneering sulfoximine insecticide and has been widely employed for managing sap-feeding insect pests across diverse crops. However, the sublethal effects of sulfoxaflor on the growth and reproduction of remain unexplored.

Foliar Spray of Cerium Oxide Nanoparticles (CeO NPs) Improves Lead (Pb) Resistance in Rice.

The widespread use of lead (Pb) has led to serious environmental and human health problems worldwide. The application of oxide nanoparticles (CeO NPs) in alleviating abiotic stress in plants has received extensive attention. In this study, 50 mg·L CeO NPs can improve Pb resistance and promote rice growth.

Human-Derived Proteome Characterization of Tongue Coating in Colorectal Cancer.

Most colorectal cancer (CRC) patients experience changes in tongue coating morphology, but the underlying mechanisms remain unclear. While the human-derived proteome of tongue coatings in gastric cancer and healthy individuals has been characterized, research on CRC patients remains lacking. The tongue coating collection process is painless and noninvasive, providing a more favorable examination experience.

Prediction of endoscopic restenosis after endoscopic balloon dilation in patients with Crohn's disease: a machine learning approach.

Background: Endoscopic balloon dilation (EBD) is recognized as a minimally invasive and effective procedure for managing intestinal stenosis in patients with Crohn's disease (CD). It offers an alternative to surgery and has been shown to improve the quality of life for these patients by reducing the need for more aggressive interventions. This study aimed to evaluate factors associated with endoscopic restenosis after EBD and construct a prognostic model.

Multivalent assembly of nucleolin-targeted F3 peptide potentiates TRAIL's tumor penetration and antitumor effects.

Tumor-targeting drug delivery holds great promise for cancer treatment but faces significant challenges in penetrating solid tumors to achieve optimal therapeutic efficacy. By harnessing the natural tissue-penetration effect conferred by the CendR motif, we identified that the nucleolin (NCL)-targeted peptide F3 possesses tumor-penetrating capabilities. Co-administration of F3 with doxorubicin and the apoptosis-inducing protein TRAIL enhanced effective tumor penetration and improved antitumor activity.

Tibetan Tea Alleviates the Intestinal Dysfunction in Sleep-Deprived Mice Through Regulating Oxidative Stress and Inflammation-Related Intestinal Metabolisms.

Sleep deprivation (SD) disrupts intestinal homeostasis through excessive reactive oxygen species (ROS) accumulation. Tibetan tea is a potential dietary intervention for inflammation, it's effect on SD-induced intestinal inflammation remains unclear. This study investigates the alleviating effects of Tibetan tea water-soluble extract (TTE) on intestinal dysfunction in SD mice.

Early Miocene ventricose bamboo from south Asia with implications for evolutionary ecology and biogeography.

The evolutionary history of bamboo has remained elusive, primarily due to the scarcity of fossils that exhibit varied morphological traits, often lacking detailed features. In this study, we introduce a remarkable fossil find, a bamboo culm from the early Miocene sediments of the Neyveli lignite mine in India. This fossil is distinguished by its nodal buds and notably ventricose (swollen) nodes-features rarely preserved in the fossil record.