Microenvironment-Programmed siRNA-Based Hydrogel for Spatiotemporal Gene Silencing in Wound Healing.

Excessive inflammation and overexpressed matrix metalloproteinases (MMPs) are significant factors in the prolonged healing of chronic diabetic wounds. Here, a precise gene therapy strategy is proposed utilizing siRNA and employing intelligent responsive materials for controlled release to mechanistically intervene in the pathological process of chronic non-healing wounds. The system employs a cationic hyperbranched aminoglycoside with disulfide bonds (SS-HPT) as its core delivery mechanism.

Lactococcus formosensis and its metabolite 4-acetamidobutanoic acid induced caspase-11 dependent myenteric neuronal pyroptosis in intractable functional constipation.

Intractable functional constipation (IFC), a severe form of chronic constipation characterized by slow transit and resistance to conventional treatments, posed a significant clinical challenge. Here, we identified Lactococcus formosensis (Lf), a Gram-positive bacterium prevalent in IFC patients, as a novel contributor to intestinal motility impairment. Clinically, IFC patients exhibited increased colonic mucosal colonization of Lf and significant myenteric neuronal loss and pyroptosis, particularly in excitatory choline acetyltransferase (ChAT) neurons, but not inhibitory neuronal nitric oxide synthase (nNOS) neurons.

The structure of a thermostable phage's portal vertex and neck complex illuminates the headful maturation mechanism.

Viruses assemble from component parts inside their host cells, but the mechanisms coordinating this complex process are not completely understood. In tailed bacteriophages, the genome is packaged into its capsid shell through the portal complex. The portal complex then closes to retain DNA and connects to the tail, which is required for host recognition and infection.

Biomass and valve length of diatoms living on tree bark as novel indicators of atmospheric environment changes.

Diatoms, an important group of primary producers, are valuable indicators of environment changes in aquatic ecosystems. Presently, limited knowledge is available on diatoms living on tree bark and their responses to atmospheric environmental changes. Mosses such as Hypnum callichroum living on the bark of Cinnamomum camphora were collected monthly in a 9-year period to explore temporal changes in composition, biomass and valve length of diatoms in a subtropical evergreen broadleaf forest.

Efficacy and Safety of Jingling Oral Liquid for Children With ADHD: A Multicenter, Randomized, Double-Blind, Placebo-Controlled Clinical Trial.

Objective: This study aims to verify the therapeutic efficacy and safety of a traditional Chinese medicine, Jingling Oral Liquid, in treating ADHD through a multicenter, randomized, double-blind, placebo-controlled trial (ChiCTR-IPR-17012293).

Method: Eighty-five children with ADHD underwent 8 weeks of strictly monitored treatment. Forty children (8.

Stage-Resolved Phosphoproteomic Landscape of Mouse Spermiogenesis Reveals Key Kinase Signaling in Sperm Morphogenesis.

Spermiogenesis is the committed step of sperm production, during which spermatid cells undergo dramatic morphological transformations and transcriptional silencing. Post-translational modifications (PTMs), including phosphorylation, provide a level of protein function flexibility and play important roles in spermiogenesis. Dynamic protein phosphorylation profiles of spermatids are characterized across four different developing steps, and identified phosphorylation regulation of key proteins in spermiogenesis.

[Expert consensus on local anesthesia application in pediatric dental therapies].

Dental treatments for children and adolescents have unique clinical characteristics that differ from dental care for adults in terms of children's physiology, psychology, and behavior. These differences impose specific requirements on the application of local anesthesia in pediatric dental procedures. This article presents expert consensus on the principles of local anesthesia techniques in pediatric dental therapies, including the use of common anesthetic drugs and dosage control, safety and efficacy evaluation, and prevention and management of complications.

Punicalin ameliorates vascular endothelial cell pyroptosis and dysfunction induced by hyperlipidemia and hyperglycemia through activation of PPARγ.

Vascular endothelial dysfunction, a form of chronic endothelial inflammation, frequently occurs in patients with diabetes, hyperlipidemia, and hyperglycemia, which is a key trigger of cardiovascular diseases. Emerging evidence has indicated that pyroptosis plays a crucial role in endothelial dysfunction and potentially represents a promising therapeutic strategy. Punicalin (PUN), a bioactive compound derived from ellagitannins, has demonstrated great potential of anti-inflammatory and anti-pyroptosis.

Correction: Evaluating multiple large language models on orbital diseases.

[This corrects the article DOI: 10.3389/fcell.2025.

Construction of risk prediction model for dysphagia in hospitalized elderly patients with frailty.

Background: Dysphagia is a common complication in elderly patients with frailty, affecting their prognosis and quality of life. Constructing a risk prediction model can help with early screening and intervention.

Objective: To investigate the current status of dysphagia in hospitalized elderly patients with frailty, analyze its influencing factors, and construct a risk prediction model for dysphagia in hospitalized elderly patients with frailty.

Deficiency of Kif15 impairing synaptic development leads to mood disorder in mice.

The harmony of neuronal excitation and inhibition is essential for precise neuronal circuitry in the developmental brain, and thus affects the human emotion. Abnormalities of synaptic morphology directly affect neuronal function and contribute to a variety of psychiatric disorders. Previous studies have shown that Kif15 (Kinesin-12), a microtubule-associated motor protein, affects neurite growth, navigation, and branching during neuronal development, revealing the potential of Kif15 to influence neuronal dendritic morphology.

Hyperbaric oxygen augments Doxil antitumor efficacy by reducing tumor-induced lactate.

Compared to healthy individuals, cancer patients exhibit significantly elevated lactate. While numerous studies have revealed the critical role of lactate in fostering an immunosuppressive tumor microenvironment, the effects of lactate on cancer nanomedicine (e.g.

Construction effect of fertile cultivated layer in albic soil. Ⅱ. Effects of deep tillage with organic materials on nutrient contents and pH in tillage layer of albic soil.

White slurry layer of albic soil has low fertility and poor nutrient availability. Organic material return to fields is a primary agricultural management practice for enhancing soil fertility. We examined the variations of soil nitrogen, phosphorus and potassium nutrient contents and pH of the topsoil layer (0-15 cm) and subsoil (15-35 cm) layers of albic soil under different treatments, including deep tillage at 35 cm without organic materials (T), straw deep mixing at 35 cm (T+S), organic fertilizer deep mixing at 35 cm (T+M), combined straw and organic fertilizer deep mixing at 35 cm (T+M+S), and conventional tillage at 15 cm without organic materials as the control (CK).

Expert consensus on the treatment of oral diseases in pregnant women and infants.

With the growing emphasis on maternal and child oral health, the significance of managing oral health across preconception, pregnancy, and infancy stages has become increasingly apparent. Oral health challenges extend beyond affecting maternal well-being, exerting profound influences on fetal and neonatal oral development as well as immune system maturation. This expert consensus paper, developed using a modified Delphi method, reviews current research and provides recommendations on maternal and child oral health management.

Tailor-Made Host-Guest Organic-Inorganic Hybrid Antimony Halide Enables Efficient Light-Emitting Diodes.

Organic-inorganic antimony (Sb) halides are garnering increasing interest for lead-free perovskite light-emitting diodes (LEDs), but the non-radiative recombination and poor charge transport are hard-treat case to restrict their electroluminescent performance. Here we developed efficient Sb halide LEDs based on the tailor-made host-guest (PhP)SbCl (PhP = tetraphenylphosphonium) emitters that enable good luminescence and charge transport properties simultaneously. Experimental and theoretical studies reveal that the self-trapped excitons triggered by excited-state structural deformation were localized in spatial-confined [SbCl] polyhedrons, generating a high photoluminescence quantum yield (96.

The cream-skimming behaviors of tertiary hospitals under medical alliances: evidence from China.

Background: While previous studies have delved into the formation and development of medical alliances in China, there has been limited focus on investigating inequity in the referral rates and the quality of care received provided to patients with experience of referral and those without under the introduction of medical alliances. This study explored: (1) inequity in the odds of being referred to healthcare institutions within medical alliances; and (2) inequity in the quality of care received between the referred and non-referred patients.

Methods: This study employed a dataset comprising 440,950 individuals who had at least one outpatient visit at healthcare facilities in Hangzhou city, Zhejiang province, China from January 1, 2020 to September 24, 2021.

Global, regional, and national burden of caries in primary teeth from 1990 to 2021: results from the global burden of disease study 2021.

Objective: To investigate the disease burden of caries in primary teeth across global, 21 regions, and 204 countries and territories from 1990 to 2021.

Methods: Data on caries in primary teeth from 1990 to 2021 were obtained from the Global Burden of Disease (GBD) 2021 Study. The global incidence, prevalence of caries in primary teeth associated disability-adjusted life years (DALYs) were analyzed.

Differential Responses of Rice Genotypes to Nitrogen Supply: Impacts on Nitrogen Metabolism and Chlorophyll Fluorescence Kinetics.

Nitrogen (N) availability significantly influences plant metabolism and productivity. The aim of this study was to assess the effects of low N stress and subsequent N supplementation on key enzymes of nitrogen metabolism, nitrogen metabolism-related substances, and chlorophyll a fluorescence kinetic parameters in rice genotypes with different nitrogen utilization efficiencies. We used the Jijing 88 (low-N tolerant) and Xinong 999 (low-N sensitive) as test materials.

Hybrid EEG Feature Learning Method for Cross-Session Human Mental Attention State Classification.

Background: Decoding mental attention states from electroencephalogram (EEG) signals is crucial for numerous applications such as cognitive monitoring, adaptive human-computer interaction, and brain-computer interfaces (BCIs). However, conventional EEG-based approaches often focus on channel-wise processing and are limited to intra-session or subject-specific scenarios, lacking robustness in cross-session or inter-subject conditions.

Methods: In this study, we propose a hybrid feature learning framework for robust classification of mental attention states, including focused, unfocused, and drowsy conditions, across both sessions and individuals.

The predictive role of peripheral serum inflammatory markers NLR, PLR, and LMR in ulcerative colitis and Crohn's disease: a systematic review and meta-analysis.

Background: The neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and lymphocyte-to-monocyte ratio (LMR) are peripheral serum markers commonly utilized as cost-effective indicators of inflammation. However, their efficacy as predictors of clinical disease activity in inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), remains uncertain. To address this ambiguity, we conducted a meta-analysis to evaluate the clinical significance of NLR, PLR, and LMR in patients with IBD.

Correction: The predictive role of peripheral serum inflammatory markers NLR, PLR, and LMR in ulcerative colitis and Crohn's disease: a systematic review and meta-analysis.

[This corrects the article DOI: 10.3389/fimmu.2025.

Reprogramming the tumor microenvironment with c-MYC-based gene circuit platform to enhance specific cancer immunotherapy.

Intratumor heterogeneity (ITH) is associated with anti-tumoral immune response and with the efficiency of cancer immunotherapy, yet overcoming ITH remains a significant challenge. Notably, cellular MYC (c-MYC) has been shown to be a pivotal orchestrator of this ITH progression. Here, we develop a c-MYC-based sensing circuit (cMSC) that is activated exclusively by aberrant c-MYC levels, along with an exosome-based cell-to-cell (CtC) system that augments communication among tumor cells, effectively targeting all cells in tumors circumventing the limitations imposed by ITH.

Highly Bright and Stable Chiral CsPbBr Perovskite Nanocrystal Scintillators for High-Resolution X-ray Imaging.

Chiral perovskite nanocrystals (PeNCs) scintillators hold great potential for reducing optical crosstalk in X-ray imaging, due to their circularly polarized radioluminescence (CPRL) properties. However, due to the weak binding of chiral ligands and inefficient chirality transfer, achieving chiral PeNCs with high radioluminescence dissymmetry factors () remains a challenge. Here, we introduce polydimethylsiloxane (PDMS) as a CPRL-enhancing modifier that anchors chiral ligands to PeNCs surfaces through σ-π interactions.